The purpose of this study was to investigate the relationships between gender, prior academic performance, beliefs and student attitudes toward biology laboratory experiences. The sample consisted of 294 students from 10th, 11th and 12th grades enrolled in a Catholic high school in a major metropolitan area in the Southeast. Two 11‐item scales were created; one to measure student attitudes toward biology laboratory experiences, and the other to measure student beliefs about the benefits of biology laboratory. A three‐way analysis of variance (gender × prior academic performance × beliefs) was conducted with the attitudes toward biology used as the dependent variable. Gender had a significant effect on attitudes, with females reporting more positive attitudes toward biology laboratory than males. Prior academic experience was also a significant predictor of attitudes; students who received lower GPAs in previous science courses reported more positive attitudes toward biology laboratory than students with higher GPAs. Based on previous research this finding was surprising; however, it appears that lower achieving students may perceive that there is a higher benefit from “hands on” laboratory experiences than high achieving students. The data also indicated that beliefs had the strongest correlations with attitudes; students who believed laboratory experiences were beneficial had more positive attitudes. The implications for research, theory and practice are also presented.
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