Considering the new reality in Russia, research needs to identify and use additional factors to ensure economic growth. The spatial factor plays an important role in enhancing the sustainability of economic development and increasing macroeconomic dynamics. The study examines the formation and assesses the development potential of Russian preferential territories, of which there are currently more than 860. The research methodology is based on the principles of spatial development, synergetic economics and institutional theories. Methods of comparative, statistical, structural, and SWOT analysis are used. It is hypothesised that the use of the development potential of preferential territories (in this case, priority development areas (PDAs) of single-industry towns) will increase their technological advancement. As of 2024, 110 Russian territories, including 84 single-industry towns, have the priority development area status. The opportunities and development risks of Russian preferential territories were analysed using SWOT. An institutional-synergetic approach to the formation of PDAs was utilised to create a methodology for determining development potentials. The performed assessment of the potential of 9 priority development areas in single-industry towns revealed the presence of technologically advanced institutions only in three of them (Naberezhnye Chelny, Tolyatti and Nevinnomyssk). Six PDAs have resources, development institutions, and infrastructure corresponding to the current technological development level of the territory. Three PDAs can rely on the strategic potential of their regions (characterised by a high or average quality of life and investment attractiveness). The study offered recommendations for adjusting territorial development strategies, taking into account the socio-economic potential of priority areas for their transition to the next technological level. The findings can be used for updating the Spatial Development Strategy of the Russian Federation and adjusting the performance indicators of preferential territories.