Principle Of Compatibility Research Articles

Development of amphiphilic hypercrosslinked porous polymers for magnetic extraction of multiple environmental pollutants in water

Under the principle of similar compatibility, researchers have developed various polarity extractants corresponding to a class of chemicals. Separating different polarities chemicals with one extractant effectively has become a novel research trend in separation science. Given the complexity of environmental sample matrices and the significant differences in polarity and solubility of various compounds, the introduction of hydrophilic groups to hydrophobic material skeletons can lead to sorbents with hydrophilic-lipophilic balance (HLB) property and thus improve their extraction performance for substances with different polarities. In this work, a hypercrosslinked polymer (HCPPz-TPB), designated as HLB, was synthesized by incorporating polar pyrazine and nonpolar triphenylbenzene molecules within each other. Subsequently, a core-shell magnetic composite material was obtained by encapsulating magnetic Fe3O4 nanoparticles in HCPPz-TPB. The material was applied as an adsorbent for magnetic solid phase extraction (MSPE) and combined with a high-performance liquid chromatography-photodiode array detector (HPLC-PDA) to enrich, separate, and detect seven polar contaminants in environmental water samples. The proposed approach, Fe3O4@SiO2@HCPPz-TPB−MSPE-HPLC-PDA, is characterized by its outstanding high sensitivity, low detection limits, wide linear range, and good reproducibility. The method demonstrated satisfactory linearity in the range of 0.05–2 μg mL-1 with R2 values between 0.9969 and 0.9997; the limits of detection (LOD) were observed to be within the range of 0.0019–0.016 μg L-1, and limits of quantification (LOQ) was observed to be within the range of 0.0064–0.054 μg L-1 range with good precision. The recoveries of the different contaminants in the environmental samples ranged from 83.61 to 116.46% (RSD≤10.56, n = 5). The new hydrophilic-lipophilic balance extractant is highly efficient, sensitive, and precise for extracting different polar pollutants. The findings demonstrate that the Fe3O4@SiO2@HCPPz-TPB display a remarkable affinity for multiple targets, driven by complex interactions including multi-stackings and hydrogen bonding as a sorbent. The synthesized Fe3O4@SiO2@HCPPz-TPB may be employed in diverse applications, including extraction, removal, and determination of diverse trace multi-target analytes in complex media.

Examining the effectiveness of interventions to reduce discriminatory behavior at work: An attitude dimension consistency perspective.

Academic interest in reducing discrimination has produced substantial research testing interventions to mitigate biased outcomes. However, disparate findings and a scarcity of studies examining work-related behavioral measures make it challenging to determine which interventions are better suited to reduce workplace discrimination. Derived from the tripartite theory of attitudes and the principle of compatibility, I develop a conceptual model mapping the attitude focus of interventions and code studies in this literature from the past two decades for these common properties. Based on a meta-analysis of 70 articles totaling 208 effect sizes, I test this conceptual model, finding that it helps explain why some interventions to reduce discrimination yield superior outcomes relative to others. In particular, results indicate that passive interventions, such as short-term education or reminders of bias processes, are largely ineffective in shifting behavior. Conversely, the class of interventions that targets behavior directly by attempting to inhibit the manifestation of bias (e.g., making individuals accountable for their decisions or changing social norms) emerged as the most helpful category of interventions in this area. Overall, results support a key prediction of the attitude dimension consistency perspective, demonstrating that aligning the attitude dimension primarily targeted by an intervention and the outcome measured could lead to improved results in this area. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Research on the adhesion mechanism and compatibility of acrylate composite polyurethane binder − aggregate based on surface free energy theory

The raw materials selection and design of waterproof polymer concrete for steel bridge deck paving lack clarity regarding the adhesion mechanism and optimal compatibility between acrylate composite polyurethane binder (APUB) and various aggregates. This study systematically investigates these issues based on surface free energy (SFE) theory. Initially, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) techniques are utilized to characterize chemical structure of APUB. Subsequently, obtaining the SFE parameters of APUB and aggregates through contact angle tests, and establishing the evaluation indices compatibility ratios ERs (ER1 and ER2) for the most compatible APUB-aggregate pairs. Finally, the correlation between ERs and the water stability of the APUB-aggregate pair is verified through shear tests conducted on sandwich specimens immersed in water. The findings reveal that APUB exhibits both acidic and non-polar surface properties. The SFE values of all aggregates surpasses that of APUB, indicating APUB's ability to wet the surfaces of all aggregates. Under dry conditions, non-polar basalt and limestone aggregates exhibit strong adhesion to APUB. This is primarily due to the combined effects of acid-base chemical reactions and the principle of compatibility, with the former predominating. Under wet conditions, APUB on non-polar basalt and limestone surfaces exhibits reduced susceptibility to displacement and detachment by water. The closer the polarity component of polar aggregates is to that of water, the easier water can displace APUB from the polar aggregate surfaces. Correlation analysis between the maximum shear force of the immersed specimens and the ER2 indicates that the APUB-basalt pair has the best compatibility. The cohesive work of APUB is crucial in establishing compatibility indicators for evaluating APUB-aggregate pairs. This study provides a theoretical basis for the raw material selection and design of polymer concrete for steel bridge deck paving.

THE GEOGRAPHICAL INTERPRETATION OF THE REGION «CENTRAL EUROPE»

Today on the political map of the world, Europe occupies a special position, plays an important role in defining and shaping the modern global world order. The full-scale war taking place in the heart of Europe indicates the relevance of the problem of civilizational confrontation in the 21st century and the difficulty in geographically defining those territories and natural regions that occupy a buffer position. Such geography not only determines the degree and rationality of the use of natural conditions and resources, but also the intensity and direction of the reconstruction of the landscape structure of the region, the transformation of natural landscapes into various groups of man-made ones, their subsequent changes and anthropogenic layering. Central Europe is a special region of Europe. This territory has always been at the forefront of scientific and political knowledge during the most difficult stages of the historical formation and development of Europe. In the 20s of the 21st century, it did not lose its importance, and the transformational processes shaping the geopolitical situation in the Northern Hemisphere and on the continent of Eurasia confirm this. A.H. Donska notes that the concept of Central Europe characterizes one of the most unstable and uneven parts of Europe. Without a clear geographical designation, Central Europe can be seen as a response to global political challenges. Scientists claim that today there is a need to rethink the regionalization of Europe, the place and role of Central Europe in the processes of dynamic interaction between the Asia-Pacific region and the European Union. The concept of Central Europe, as a component of the concept of Europe, is the spread of the European integration process, Europeanness and civilizational choice. The aim of the article: to explore geographical interpretations of the Central Europe as a scientific category. The study was based on the principle of natural anthropogenic compatibility, which is thoroughly revealed in the works of G. I. Denysyk. General and specific scientific research methods have been used. The article scientifically substantiates and proves that Central Europe is a natural-geographical, socio-geographical, geopolitical and historical-geographical region, which today should be interpreted as a spiritual concept, state of mind, geographical bastion, appropriate institutional basis, as a struggle for "its western" and understanding of Europeanness. The novelty of the study is that Central Europe was considered not only as a geographical materialist territorial entity (natural, social-geographical), but also from the point of view of humanistic geography as a natural-socio-historical-cultural territory, which is constantly changing in the space and time. Key words: Central Europe, natural-geographical region, socio-geographical region, natural-socio-historical-cultural territory.

Discrimination of anti-tumor and cardioprotective effect quality markers from Aidi Injection based on "spider web" mode

Chinese medicinal preparations play an equally important role in reducing toxicity and treating tumors. Few studies discriminate the quality markers(Q-markers) conferring different therapeutic effects of traditional Chinese medicine preparations. Therefore, we take Aidi Injection(AD) as an example to comprehensively identify the Q-markers of anti-tumor and cardioprotective effects based on the "spider web" mode. Firstly, based on the principle of measurability, the chemical components in the prescription were qualitatively analyzed, and then the components with high content and capable to be measured were quantitatively analyzed as measurable evaluation indexes. Based on the principle of stability, the effects of light and temperature on the content of each component of AD were investigated as indicators of stability. Based on the principle of compatibility, the compounds were classified according to the law of compatibility of sovereign, minister, assistant, and guide medicinal materials in the prescription. Based on the principle of efficacy, the anti-tumor and antiangiogenic activities of the Q-markers were evaluated, and their synergistic effects with doxorubicin(DOX) in inhibiting tumorigenesis and angiogenesis and lowering cardiotoxicity were evaluated as the evaluation indexes of effectiveness. The seven-dimensional spider web of "compatibility-content-stability-antitumor activity-synergistic anti-tumor activity with DOX-antiangiogenic activity-synergistic anti-angiogenic activity with DOX" and the four-dimensional spider web of "compatibility-content-stability-protective effects against DOX-induced myocardial toxicity" were established, on the basis of which the Q-markers of anti-tumor and cardioprotective effects of AD were comprehensively analyzed. The results showed that 12 components were selected as the Q-markers of AD, among which cantharidin, ginsenoside Re, ginsenoside Rb_1, astragaloside Ⅱ, cryptochlorogenic acid, and ginsenoside Rg_2 were the anti-tumor Q-markers of AD. Ginsenoside Rd, isofraxidin, syringin, eleutheroside E, calycosin-7-O-β-D-glucoside, and azelaic acid were the cardioprotective Q-markers of AD. Taking into account both the anti-tumor and cardioprotective effects, these Q-markers could cover the four herbs constituting the prescription. The findings provides a scientific basis for the quality control of AD and an effective method for identifying comprehensive and reasonable Q-markers for the two effects of Chinese medicinal preparations.

Experimental and molecular simulation analysis of the dissolution behavior of Musk ambrette in organic solvents

The solubility of Musk ambrette in i-propanol (IPA), i-butanol (IBA), n-heptane (HPT), n-hexane (HX), cyclohexane (CYH), methyl acetate (MAC), ethyl acetate (EAC), acetone (ACE), IPA + ACE at 278.15 K to 318.15 K was determined. The mole fraction solubility of Musk ambrette in pure IPA at the temperature of 298.15 K were found to be 0.9492 × 10-2 mol·mol−1. The difference is that the solubility of Musk ambrette in ACE reaches 0.2257 mol·mol−1, which is much higher than the solvent mentioned above. The strong intermolecular hydrogen bonding formed by IPA resulted in its lowest solvation capacity for Musk ambrette. In addition, to further investigate the solubility pattern of Musk ambrette, we used 8 models (Van’t Hoff, modified Apelblat, Buchowski-Ksiazczak, Yaws, NRTL, Wilson, Jouyban-Acree and Sun model) to fit and obtain a general equation for solubility versus temperature. These results yielded a large amount of statistically significant data. Comparisons revealed that the modified Apelblat and Yaws equation showed the least deviation in forecasting solubility variations with temperature. Meanwhile, we proved that the dissolution of Musk ambrette is a heat-absorbing entropy increasing process by Van't Hoff equation calculation. ΔsolGo for the 12 solvents is in the following order: ACE < MAC < EAC < w = 0.2007 < w = 0.4025 < w = 0.6076 < CYH < w = 0.8070 < HPT < HX < IBA < IPA. ΔsolGo is smaller the easier it dissolves, which is consistent with the law of dissolution. Secondly, we performed thermal analysis and X-ray diffraction tests on Musk ambrette crystals prepared in different solvents, which showed that none of the several systems investigated led to the creation of new crystalline forms or solvatoids, further confirming the suitability of the solvents. Besides, we discuss the correspondence between solubility and the HSP, π, α, β, and CED of the solvents and thus tentatively confirm the significant influence of the solvent properties on solubility. The principle of similarity and compatibility is satisfied for most of the solvents. In particular, the CED values of the larger solvents were not favorable for the solubilization of Musk ambrette. We also analyzed the molecular surface electrostatic potential of Musk ambrette by visualization techniques. Finally, IPA and ACE and their binary solution systems were analyzed by molecular dynamics simulations. The effect of solvent intermolecular interactions on the solubilization pattern was discussed. It was further confirmed that weak interactions of solvent molecules contribute more to the dissolution of Musk ambrette. These findings suggest that the prediction of solvent solubility can be initially achieved by molecular simulation techniques. Undoubtedly, this study will furnish a valuable information for the purification of Musk ambrette.

Experimental study of ultra-low IFT foam flooding for low permeability reservoirs

Foam flooding is an effective oil displacement technique to improve fluid sweep efficiency and increase oil recovery. The objective of this paper is to investigate the applicability of ultra-low IFT foam flooding for low permeability oil recovery under the conditions of WX reservoir block in Tuha oilfield (84°C, 86,072 mg/L). A modified amphoteric hydroxy sulfobetaine surfactant (Cn-HSB) was formulated as a foaming agent based on the principle of compatibility with crude oil that could achieve the ultra-low interfacial tension (IFT) and strong foamability simultaneously. This is shown to reduce the oil/water dynamic IFT (DIFT) to an ultra-low value of the order of 10−5 mN/m (0.10wt%), as well as produce a foam volume and half-life of 448 mL and 2567 s, respectively. In addition, results of IFT and foam quality tests indicate that the modified Cn-HSB has good thermal stability and excellent salinity-resistance. Moreover, the ultra-low IFT foam flooding has an ideal injectivity and oil displacement efficiency in low permeability porous media that can mobilize the trapped oil in k = 1.50 × 10−3μm2 with a threshold pressure gradient of 0.115 MPa/m. This improves oil displacement efficiency about 15%OOIP. Fundamental knowledge gained from this research gives insight into the application of ultra-low IFT foam flooding in low-permeability reservoirs under a combination of specific reservoir conditions.

Laser-welded butt joints of Ti2AlNb/42CrMo steel with addition of V interlayer

Welding Ti2AlNb and 42CrMo steel is important in the field of materials engineering. The formation of Ti–Fe intermetallic compounds upon melting makes it a challenging task due to the significant disparities in the physical properties and chemical compositions of these two materials. An orthogonal experiment was designed and conducted, and crack-free laser-welded butt joints of Ti2AlNb/42CrMo steel were achieved under optimized parameters. The joints exhibited a tensile strength of approximately 162 MPa. Fracture surface analysis revealed substantial amounts of Fe2Ti and FeTi intermetallic compounds within the weld zone. According to the principle of element compatibility, a method is proposed for incorporating a partially melted V interlayer for laser butt welding of Ti2AlNb/V/42CrMo steel. The laser beam was vertically incident on the interface between Ti2AlNb and V, as well as the interface between V and steel. No visible microcracks were observed after welding. Elemental analysis results indicated that the V interlayer prevented the formation of the Fe–Ti mixed zone. Furthermore, the weld zone primarily consisted of (Fe, V) solid solution and (β-Ti, V) solid solution; thus, Ti–Fe intermetallic compounds were not formed. The tensile strength of the joint reached 572 MPa, and the joint fractured within the unmelted V layer, exhibiting a typical ductile fracture morphology.

Equilibrium solubility and thermodynamic study of daminozide in twelve neat solvents from (283.15 to 323.15) K

In the present study, solubility of daminozide was determined in twelve neat solvents (N,N-Dimethylformamide (DMF), 2-methoxyethanol, N-Methyl-2-pyrrolidone (NMP), 2-ethoxyethanol, Acetonitrile (MeCN), 2-propoxyethanol, methanol, ethanol, n-propanol, i-propanol, n-pentanol, 2-butoxyethanol) by using the gravimetrical method under the pressure of 0.1 MPa and temperature from 283.15 to 323.15 K. The maximum solubility value (102x1) of daminozide was found in NMP at 323.15 K (10.04), while the minimum solubility value (102x1) of daminozide was found in n-pentanol at 283.15 K (0.0149). At the temperature of 298.15 K, the order of the experimental solubility values (100x1) of daminozide in twelve neat solvents was: NMP (5.523) > DMF (3.294) > methanol (1.395) > 2-methoxyethanol (0.7839) > ethanol (0.5515) > 2-ethoxyethanol (0.3816) > n-propanol (0.2175) > i-propanol (0.1099) > 2-propoxyethanol (0.1083) > 2-butoxyethanol (0.0632) > MeCN (0.0386) > n-pentanol (0.0344). Five models including van't Hoff model, Buchowski-Ksiazczak λh model, NRTL model, UNIQUAC model and Jouyban model were employed to correlate and analyze the experimental solubility of daminozide. Based on the principle of similar compatibility, the miscibility of daminozide in the selected solvents was discussed with the help of Hansen solubility parameters (HSPs). Finally, the apparent thermodynamic properties were investigated and results indicated that the dissolution mechanism of daminozide in all selected solvents was an entropy-driven and endothermic process.

Судьба ученого: Башир Искандарович Рамеев

The article is dedicated to the life and work of Bashir Iskandarovich Rameev, one of the founders of domestic computer technology in the USSR, whose professional activities were closely connected with the city of Penza. In the 1950s, the largest research institute for electronic computing machines was established based on the SAM plant in Penza. The article focuses on the scientist's life path, which reflects the history of the country – the revolution of 1917, repressions, the Great Patriotic War, the achievements of Soviet science and technology in the post-war years in the setting of the Cold War. The formation of B.I. Rameev as a scientist coincided with the development of the first information technologies – the development of the first domestic computer “Strela”. The creation of a galaxy of electronic computing machines “Ural” falls on the peak of his career, when the scientist fullfilled the “principle of programmatic and constructive compatibility”.

Transformational leadership and organizational citizenship behavior: new mediating roles for trustworthiness and trust in team leaders

This study investigates the pivotal role of trust in bridging the effects of transformational leadership on organizational citizenship behavior (OCB). The study was conducted using a multilevel longitudinal approach with 276 employees in 71 teams from private medium-sized organizations in Kuala Lumpur, Malaysia. Transformational leadership was found to be positively related to: (1) three facets of trustworthiness (ability, benevolence, and integrity); (2) trust in the leader; and (3) OCB. All three facets of trustworthiness mediated the relationship between transformational leadership and trust in leaders. In addition, trust in the leader mediated only the relationship between the benevolence facet of trustworthiness and OCB. As OCB is inherently benevolent, these findings not only are consistent with the principle of compatibility, but they also contribute to theorizing about ‘how’ trust plays an important role in the influence of transformational leadership on employees.

Sodium alginate coating of Ginkgo biloba leaves extract containing phenylpropanoids as an ecofriendly preserving agent to maintain the quality of peach fruit.

Plant constituents are of great interest in the food processing industry as potential natural preservative agents for controlling foodborne pathogens. In this study, the 95% EtOH/H2 O extract of Ginkgo biloba leaves was separated using polarity extraction solvents with petroleum ether (PE), ethyl acetate (EA), n-butanol (nB), and water (W) by the principle of similarity and compatibility. Through TLC and NMR analysis of these extracts, it can be concluded that the main component of PE extract were organic acids, for EA extract were flavonoids, for nB extract were phenylpropanoids, and water extract were oligosaccharides. Twelve monomer compounds were separated from the extracts to verify the composition of each extraction stage. Results of morphological and molecular identification revealed that Monilinia fructicola and Rhizopus stolonifer were the main fungi causing peach rot. After evaluating the antifungal activity and peach quality of the four extract/sodium alginate coatings, it was found that the n-butanol extract/sodium alginate coating containing phenylpropanoids had the lowest decay index and the best preservation effect, providing a sustainable alternative to reduce the harm to the environment of synthetic preservatives. PRACTICAL APPLICATION: The abuse of synthetic preservatives poses a threat to the ecological environment and physical health. Therefore, this study developed sodium alginate coating of Ginkgo biloba leaves extract containing phenylpropanoids, which has good effects on the preservation of peaches. The agent is a promising environmentally friendly alternative for synthetic preservatives.

Anti-interference Performance of the Temperature Acquisition System for the Vacuum Thermal Test

The thermal test temperature acquisition system is one of the core systems in the thermal test of the spacecraft. It uses thermocouple sensors for temperature data acquisition, and its data stability directly affects the success or failure of the thermal test task. The equipment types in the original thermal test environment were single, and the electromagnetic compatibility design was weak. With the increase of the types of test equipment in the thermal test, the electromagnetic interference in the test environment is becoming more and more serious and even directly affects the stability of temperature data collection. Based on the principle of thermocouple temperature measurement and electromagnetic compatibility, this paper establishes relevant models for analysis and simulation and obtains methods to improve system electromagnetic compatibility under different interference conditions. And in a certain thermal test, using the simulation results of the model, the corresponding circuit improvement was carried out, which finally reduced the influence of electromagnetic interference on the system and ensured the smooth progress of the task.

"Rater training" re-imagined for work-based assessment in medical education.

In this perspective, the authors critically examine "rater training" as it has been conceptualized and used in medical education. By "rater training," they mean the educational events intended to improve rater performance and contributions during assessment events. Historically, rater training programs have focused on modifying faculty behaviours to achieve psychometric ideals (e.g., reliability, inter-rater reliability, accuracy). The authors argue these ideals may now be poorly aligned with contemporary research informing work-based assessment, introducing a compatibility threat, with no clear direction on how to proceed. To address this issue, the authors provide a brief historical review of "rater training" and provide an analysis of the literature examining the effectiveness of rater training programs. They focus mainly on what has served to define effectiveness or improvements. They then draw on philosophical and conceptual shifts in assessment to demonstrate why the function, effectiveness aims, and structure of rater training requires reimagining. These include shifting competencies for assessors, viewing assessment as a complex cognitive task enacted in a social context, evolving views on biases, and reprioritizing which validity evidence should be most sought in medical education. The authors aim to advance the discussion on rater training by challenging implicit incompatibility issues and stimulating ways to overcome them. They propose that "rater training" (a moniker they suggest be reserved for strong psychometric aims) be augmented with "assessor readiness" programs that link to contemporary assessment science and enact the principle of compatibility between that science and ways of engaging with advances in real-world faculty-learner contexts.

The equilibrium analysis and potential modifications on the China pilot electricity spot market

The rapid development of the electricity industry reform in China has been witnessed in recent years, and one of the iconic achievements could be the establishment and trial operation of the electricity spot markets. However, even though the market clearing model for electric energy is identical to that adopted in the mature markets in the U.S., which has been widely confirmed to respect the principle of incentive compatibility, some special features are instead observed regarding the offering behaviors of generators, indicating the inconsistency with the principle of incentive compatibility. Therefore, an in-depth study on the causes for the observed features is of urgent significance, and corresponding potential modifications on the market mechanism are anticipated. This paper first establishes an equilibrium analysis model for the electric energy and frequency regulation ancillary service (FRAS) markets, based on the real-world practice in China. By providing the optimal offering strategies of the generators at the equilibrium point, the equilibrium analysis could shed light on the causes for the observed features of the offering behaviors. Then, the paper proposes potential modifications on the market mechanism considering the real institutional and operational characteristics in China, with proposing a multi-objective optimization model and a decentralized algorithm for market clearing, as well as giving full consideration to the availability of FRAS. Finally, the feasibility and effectiveness of the proposed model and algorithm are justified by case studies. The results demonstrate that the proposed modifications could achieve the identical objective of realizing the minimum system costs as the existing market mechanism adopted in the U.S., and be more adaptive to the real institutional and operational characteristics in China. Policy recommendations are also presented for the establishment and operation of the electricity spot markets in China.

Extraction and separation characteristic stilbene compounds from pigeon pea leaves using V-type thymol-based natural deep eutectic solvent systems

In this study, a V-type deep eutectic miniature solvent composed of thymol/coumarin was designed based on the principle of similitude compatibility and π-π conjugate effect for the extraction of stilbene structure compounds from pigeon pea leaves. According to Hansen solubility parameter theory, the optimal DES type and composition molar ratio were screened out. The optimal extraction conditions were thymol/coumarin ratio 2:1, ultrasonic power 200 W, ultrasonic time 20 min, liquid-solid ratio 50:1 (mL/g). According to the hydrophobic DES form hydrogen bond position, we found that add a small amount of ammonia solution can effectively destroy DES hydrogen bonding structure, which reduces the target product solubility in the solvent, combined with macroporous resin adsorption process can be quickly and efficiently recycling target component and separation solvent. Through the rotary evaporator to remove water, HBAs and HBDs are recombined to form DES, which can be directly used for secondary extraction. After three cycles of DES, the rate of recovery of DES was over 80%. Moreover, the FT-IR, UV–vis and NMR were applied to investigate physicochemical properties of DES and extraction mechanism. This study provides a new solvent screening idea for the extraction and separation of natural products, and designs a green and sustainable extraction and separation process of natural products, and provides a new idea for the recycling of hydrophobic DES.

A mechanism of Sijunzi decoction on improving intestinal injury with spleen deficiency syndrome and the rationality of its compatibility.

Sijunzi Decoction (SJZD) is a renowned formula for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM). Its non-polysaccharides (NPS) component, dominated by various compounds of SJZD, has shown the remarkable efficacy in SDS, especially in gastrointestinal injury. However, the principle of compatibility of SJZD and the micro-mechanism of effect on SDS are still unclear. To elucidate the scientific implications of SJZD compatibility and its micro-mechanism in the treatment of SDS-induced intestinal injury. First, the chemical composition of NPS in SJZD and incomplete SJZD (iSJZD, including SJZD-R, SJZD-A, SJZD-P, SJZD-G) were comprehensively analyzed by UPLC-QTOF-MS, and comparing their chemical composition by multivariate statistical analysis to reveal the effect of a single herb on SJZD compatibility. Second, network pharmacology and molecular docking were used to uncover the micro-mechanisms of potential active compounds in SJZD for the treatment of SDS, and develop an active component combination (ACC) by accurate quantification. Subsequently, the action of the potential active compounds and ACC was verified through in vivo and in vitro. A total of 112, 77, 93, 87, and 67 compounds were detected in NPS of SJZD, SJZD-R, SJZD-A, SJZD-P, and SJZD-G, respectively. Changes in the chemical components of SJZD_NPS and iSJZD_NPS revealed that RG and RAM, as well as RAM and Poria significantly affected the dissolution of each other's chemical components, and the co-decoction of four herbs promoted the dissolution of the active compounds and inhibited toxic compounds. Furthermore, network pharmacology showed that 274 compounds of 15 categories in SJZD_NPS acted on the 186 key targets to treat SDS by inhibiting inflammation, enhancing immunity, and regulating gastrointestinal function and metabolism. Finally, through in vitro experiments, six compounds among 18 potential compounds were verified to markedly repair intestinal epithelium injury by modulating the FAK/PI3K/Akt or LCK/Ras/PI3K/Akt signaling pathway. It is worth mentioning that ACC, composed of 11 compounds accurately quantified, demonstrated significant in vivo treatment effects on intestinal damage with SDS similar to NPS or SJZD. This study elucidates the scientific evidence of the "Jun-Chen-Zuo-Shi" and "detoxification and synergistic" in the decocting process of SJZD. An ACC, the active component of SJZD, ameliorate SDS-induced intestinal injury by the FAK/PI3K/Akt signaling pathway, which provides a strategy for screening alternatives to effective combinations of TCMs.

Online pharmaceutical process analysis of Chinese medicine using a miniature mass spectrometer: Extraction of active ingredients as an example

The automation of traditional Chinese medicine (TCM) pharmaceuticals has driven the development of process analysis from offline to online. Most of common online process analytical technologies are based on spectroscopy, making the identification and quantification of specific ingredients still a challenge. Herein, we developed a quality control (QC) system for monitoring TCM pharmaceuticals based on paper spray ionization miniature mass spectrometry (mini-MS). It enabled real-time online qualitative and quantitative detection of target ingredients in herbal extracts using mini-MS without chromatographic separation for the first time. Dynamic changes of alkaloids in Aconiti Lateralis Radix Praeparata (Fuzi) during decoction were used as examples, and the scientific principle of Fuzi compatibility was also investigated. Finally, the system was verified to work stably at the hourly level for pilot-scale extraction. This mini-MS based online analytical system is expected to be further developed for QC applications in a wider range of pharmaceutical processes.

Risk Assessment of Annular Pressure Caused by Tubing Leakage in Offshore Gas Wells with High CO2

CO2 sequestration is a kind of technology that can effectively mitigate the greenhouse effect, and the wellbore integrity of the storage system is the key issue to ensure successful CCUS. Failure to timely diagnose tubing leakage in offshore gas wells with high CO2 will lead to annular pressure risk and wellbore corrosion problems. The annular pressure caused by tubing leakage is characterized by high pressure and rapid rise rate, resulting in wellhead jacking, gas leakage, and wellbore structure corrosion, which has become the main cause of wellbore integrity failure in offshore gas wells. Therefore, a model of heat and mass transfer and distribution in offshore gas wells was established firstly, and the physical process of gas leakage and accumulation was described based on the pinhole model and the principle of gas PVT characteristics and volume compatibility. The results show that the depth and equivalent size of the leakage point are two important factors affecting the pressure rise process and leakage rate. Taking case well parameters as an example, after inversion calculation, the annulus pressure in case well was 23.0 MPa, the leakage point equivalent diameter was 2.3 mm, the maximum leakage rate was 0.30 m3/min, and the wellbore safety risk is relatively high. Mechanical repair and chemical plugging agent are recommended to seal the tubing leakage. These risk assessment technologies provide reference for wellbore integrity design and management to reduce CO2 leakage risk caused by wellbore integrity failure.

Compatibility between cold-natured medicine CP and hot-natured medicine AZ synergistically mitigates colitis mice through attenuating inflammation and restoring gut barrier

Ethnopharmacological relevanceUlcerative colitis (UC) is a nonspecific intestinal inflammation with complex pathogenesis. Traditional Chinese Medicine (TCM) formula consists of several TCM herbs following the principle of herbal property and compatibility. Our previous studies found that Huanglian Ganjiang decoction (HGD) exhibited anti-colitis capacity and the compatibility between hot-natured medicine and cold-natured medicine was main compatibility. However, the association between compatibility mechanism of HGD and its anti-colitis effect has not been fully illustrated yet. Aim of studyHere, we would explore whether cold-natured medicine Coptis chinensis Franch. plus Phellodendron chinense C.K.Schneid. (CP) and hot-natured medicine Angelica sinensis (Oliv.) Diels plus Zingiber officinale Roscoe (AZ) in HGD respectively produce different impacts on UC, and exert synergistic effect on UC together. Materials and methodsUPLC/MS-MS was used to qualitatively analyze chemical profiles of CP, AZ and CPAZ extracts. CPAZ-UC target network was constructed using network pharmacology. Colitis mice was induced by 3% DSS for 7 days and treated with CP, AZ and CPAZ for another 7 days. The levels of multiple cytokines and proportions of innate and adaptive immune cells were determined to assess inflammatory profiles. The leakage of FITC-dextran, expressions of tight junction proteins were detected for evaluation of gut barrier function. ResultsCP, AZ and CPAZ could improve symptoms of colitis mice. CP showed superiority in reducing proportions of pro-inflammatory immune cells M1 cells, neutrophils, Th1 and Th17 cells, and levels of pro-inflammatory cytokines IFN-γ, IL-6, IL-10, TNF-α. In the contrast, AZ had advantage of elevating ratios of anti-inflammatory immune cells M2 and Treg cells as well as the production of anti-inflammatory cytokines IL-10 and TGF-β. In addition, CP and AZ synergistically regulated M1/M2 macrophage polarization and the following IL-6, IL-10, TNF-α, IFN-γ production, thereby restoring intestinal mucosal barrier. ConclusionTaken together, our study first demonstrated that cold-natured medicine CP and hot-natured medicine AZ took on different functions in treatment of colitis mice. Meanwhile, they exhibited synergistic effect on the alleviation of intestinal inflammation and reinforcement of gut barrier function and integrity.