A mechanism of Sijunzi decoction on improving intestinal injury with spleen deficiency syndrome and the rationality of its compatibility.

Multi-omics integrated analyses indicated that non-polysaccharides of Sijunzi decoction ameliorated spleen deficiency syndrome via regulating microbiota-gut-metabolites axis and exerted synergistic compatibility

Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms

In order to clarify the traditional Chinese medicine(TCM) syndrome distribution and pathogenesis of irritable bowel syndrome(IBS), the patients in the first affiliated hospital of Guangzhou university of Chinese medicine were enrolled for the cross-sectional study. The data of 12 sociological variables, 13 risk factors, 84 symptoms and signs variables(in 9 aspects), and 19 neuroendocrine indices were extracted for group-between analysis with one-way ANOVA, chi-square test and nonparametric test, and the relationship analysis between clinical symptoms and diseases sub-types was done with binary Logistic regression. In addition, the patterns of TCM syndromes were divided by several syndrome factors to analyze the difference in neuroendocrine indices between various patterns and syndrome factors. A total of 383 IBS patients were enrolled, including 353(92.2%) cases of diarrhea, 14(3.7%) cases of constipation and 16(4.1%) cases of mixed types. In IBS-diarrhea patients, there were 291(76.0%), 18(4.7%), 48(12.5%) and 26(6.8%)cases of syndrome of liver depression and spleen deficiency (sLDSD), syndrome of liver depression and qi stagnation (sLDQS), syndrome of dampness-heat in the spleen and stomach (sDHSS), and syndrome of spleen deficiency with dampness encumbrance (sSDDE) respectively. There was significant differences in blood groups between IBS-diarrhea patients, IBS-constipation patients and IBS-mixed types patients; their disease classification was significantly correlated with the allergies, drinking, irregular meals habits, no or less vacations, and other causes of morbidity (P<0.05, f<0.3). A total of 15 symptoms and signs variables (e.g., chills, facial abnormalities, epigastric fullness, etc.) had significant differences between different groups (P<0.05), and 5, 8, 5 variables were respective independent factors for IBS-diarrhea, constipation and mixed type. There was no significant difference in neuroendocrine indices between various groups. The sLDSD, sLDQS, sDHSS, sSDDE patients had significant differences in genders, living conditions and occupations, and the TCM syndrome type was significantly correlated with the drinking, smoking, no or less breakfast, less than 8 sleeping hours(P<0.05, f<0.3). Meanwhile, a total of 14 symptoms and signs variables (e.g., dysphoria heat, fatigue, stretching, etc.) had significant differences between various groups(P<0.05) and 3, 4, 6, 3 variables were respective independent factors for sLDSD, sLDQS, sDHSS, and sSDDE. There were significant differences in acetylcholine(Ach) and angiotensin Ⅱ(AT-Ⅱ) between the sLDSD group and sSDDE group. There were significant difference in Ach, AT-Ⅱ, adrenotrophin(ACTH) and estradiol (E2) in comparison between several pattern factors. This study preliminary identified the sociological characters, risk factors, syndromes distribution, diseases and subgroup mechanisms of this disease. More samples and multi-centers are required for future study to improve the scientificity and representativeness.

Label-free mass spectrometry-based proteomics for investigating the therapeutic mechanism of Sijunzi decoction on spleen deficiency syndrome

Metabolomics analysis reveals the mechanisms of the effect of Sijunzi decoction on spleen deficiency syndrome in a rat model

The compatibility rationality of Sijunzi decoction based on integrated analysis of tissue distribution and excretion characteristics in spleen deficiency syndrome rats

Appraisal of treatment outcomes in integrative medicine using metabonomics: Taking non-alcoholic fatty liver disease with spleen deficiency syndrome as an example

Purpose: To explore the therapeutic effect and mechanism of action of Si–jun–zi decoction (SJZD) in rat spleen deficiency syndrome.Methods: Spleen deficiency syndrome was induced in a rat model with reserpine. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) and partial least squaresdiscriminate models (PLS-DA) were used to analyze the difference in global metabolite profile within all groups. Variable importance projection (VIP &gt; 1) and Student’s t–test (p &lt; 0.05) were used for biomarker selection.Results: Enzymatic results showed that the level of amylase (AMY), pepsin (LPS) and pepsase were significantly lower in the untreated rats than in normal control rats and SJZD group rats (P &lt; 0.05). Histopathological analysis showed there were significant pathological changes in the untreated rats compared with the normal control group and SJZD group rats. PLS-DA score plots of the untreated group rats clustered separately from control rats, whereas SJZD group rats clustered closely to normal control rats. Twenty potential biomarkers, including phospholipid and fatty acids, were screened. The relative content of 20 metabolites showed significant differences in the untreated group compared with control and SJZD groups (p &lt; 0.05). Six potentially perturbed metabolic pathways were found, which might be potential targets pathways for SJZD intervention in spleen deficiency syndrome.Conclusion: Metabolomics approach is an effective tool for understanding the therapeutic mechanisms of SJZD.Keywords: Si–jun–zi decoction, Spleen deficiency syndrome, Metabolomics, LC-Q-TOF/MS

Drug-polysaccharide/herb interactions and compatibility rationality of Sijunzi decoction based on comprehensive pharmacokinetic screening for multi-components in rats with spleen deficiency syndrome

Analysis of plasma proteome from cases of the different traditional Chinese medicine syndromes in patients with chronic hepatitis B

Plasma metabolic profiling on postoperative colorectal cancer patients with different traditional Chinese medicine syndromes

Background: The recurrence of colorectal adenomas (CRAs) after endoscopy predisposes patients to a risk of colorectal cancer. Guided by the traditional Chinese medicine (TCM), patients with colorectal diseases usually manifest with spleen deficiency syndrome (SDS) and are treated with Sijunzi decoction (SJZD). Therefore, this trial aims to explore the efficacy and safety of SJZD in the prevention and treatment of CRAs recurrence. Methods: SJZD on prevention and treatment of CRAs recurrence after resection: a multicenter, randomized, double-blind, placebo-controlled trial was designed. Patients who undergo polypectomy of CRAs will be recruited and randomized into a SJZD group and a placebo group in a 1:1 ratio. The intervention phase will be 12months. The follow-up period will last 24months. The primary outcome is the CRA recurrence rate after intervention. The secondary outcomes include the CRA recurrence rate at the second year post-polypectomy, the pathological type of adenoma and the alterations in SDS scores after intervention. Discussion: Previous clinical practice has observed the sound effect of SJZD in the context of gastrointestinal diseases. A number of experiments have also validated the active components in SJZD. This trial aims to provide tangible evidence for the usage of SJZD, hoping to reduce the recurrence of CRAs.

Sijunzi decoction, a classical Chinese herbal formula, improves fatigue symptoms with changes in gut microbiota in chronic fatigue syndrome: A randomized, double-blind, placebo-controlled, multi-center clinical trial

Objectives The objective of this study was to explore the correlation between the distribution of traditional Chinese medicine (TCM) syndromes and molecular types of breast cancer in the perichemotherapy period. Methods A total of 325 cases with perichemotherapy breast cancer was classified according to syndrome differentiation in TCM , and R × C table χ2 test was used to examine and analyze the relationship between TCM syndromes and molecular types of breast cancer in the perichemotherapy period. Results (1) In the early stage of chemotherapy, there was no significant difference in the distribution of different TCM syndromes among molecular types, mainly liver depression syndrome and liver depression and phlegm coagulation syndrome (p &gt; 0.05). (2) In the middle stage of chemotherapy, there were significant differences in the distribution of spleen deficiency and phlegm-dampness syndrome among HER-2 positive (HR positive), HER-2 positive (HR negative), and Luminal A type, Luminal B type (HER-2 negative), and triple-negative type (p &lt; 0.01). (3) After chemotherapy, there were significant differences in the distribution of spleen and kidney yang deficiency syndrome and marrow sea insufficiency syndrome among HER-2 positive (HR negative), triple-negative type, and HER-2 positive (HR positive), Luminal A type, Luminal B type (HER-2 negative), and triple-negative type (p &lt; 0.01). Conclusion (1) In the middle stage of chemotherapy, HER-2 positive (HR positive) and HER-2 positive (HR negative) are more likely to show spleen deficiency and phlegm-dampness syndrome than other molecular types. (2) In the late stage of chemotherapy, the HER-2 positive (HR negative) and triple-negative type is more likely to show spleen-kidney yang deficiency syndrome than other molecular types, and the triple-negative type is more likely to show marrow sea insufficiency syndrome than other molecular types.

Objective: To offer reference data for standardized study the clinical syndrome differentiation for the senile patients of post lumbar surgery, we study on the feature of Traditional Chinese Medicine(TCM) syndrome differentiation for the senile patients of post lumbar surgery. Methods: Adopting clinical epidemiological method, clinical investigation on 359 senile patients of post lumbar surgery was carried out. We designed the clinical syndrome questionnaire and filled in, acquired the data, then analyzed the data with descriptive statistics. Results: On the first day of post lumbar surgery, according to constituent ratio, the syndromes were mainly: syndrome of spleen deficiency and blood stasis(20.7%), syndrome of qi stagnation and blood stasis(19.8%), syndrome of qi deficiency with blood stasis, syndrome of spleen-stomach weakness, syndrome of damp abundance due to spleen asthenia, syndrome of dual deficiency of qi and blood. On the third day of post lumbar surgery, according to constituent ratio, the syndromes were mainly: syndrome of qi deficiency with blood stasis(35.6%), syndrome of spleen deficiency and blood stasis(21.9%), syndrome of damp abundance due to spleen asthenia, syndrome of qi stagnation and blood stasis, syndrome of spleenstomach weakness, syndrome of kidney deficiency and blood stasis. On the seventh day of post lumbar surgery, according to constituent ratio, the syndromes were mainly: syndrome of qi deficiency with blood stasis(36.6%), syndrome of qi stagnation and blood stasis(18.8%), syndrome of damp abundance due to spleen asthenia, syndrome of blood deficiency, syndrome of kidney deficiency and blood stasis, syndrome of spleen deficiency and blood stasis. On the fourteen day of post lumbar surgery, according to constituent ratio, the syndromes were mainly: syndrome of qi stagnation and blood stasis(29.2%), syndrome of dual deficiency of qi and blood(28.2%), syndrome of qi deficiency with blood stasis, syndrome of kidney deficiency and blood stasis, syndrome of spleen deficiency and blood stasis, syndrome of liver-kidney yin deficiency. Conclusions: The dynamic change law of early syndrome for the senile patients of post lumbar surgery was from deficiency syndrome to deficiency with excess complication syndrome, then to the excess syndrome.