Fatty acid composition was determined in various developmental stages of two strains of Anopheles freeborni Aitken, one from the State of Washington (47° N lat.) and one from California (37° N). Tentative identification was based on similar emission peaks from gas chromatograms of test materials and authentic samples. Palmitic, palmitoleic, and oleic acids collectively comprised at least 70% of total fatty acid. In both strains, aged females with identical temperature experience on short photoperiod had 11%–15% greater levels of principal unsaturated fatty acids than did females on long photoperiod conditions. Though differences in level were noted between sexes and various growth stages of the 2 strains, there was marked similarity between short photoperiod females of both strains maintained on sucrose for 10 days.
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