Primiparous Buffaloes Research Articles

Percentage of primiparous water buffalo cows becoming pregnant in the first 90 days postpartum

Reproductive performance is a critical factor affecting the profitability of buffalo systems, and parity is a factor affecting reproductive performance. This study aimed to evaluate the effect of parity on the percentage of pregnant buffaloes for day 90 postpartum and determine if the postpartum treatment with GnRH increases the reproductive efficiency of primiparous water buffalo cows. In the first experiment, water buffalo cows from one commercial farm located in Coloncito, Tachira, Venezuela, were grouped into primiparous (n=77) or multiparous (n=341), and the percentage of pregnant buffaloes at day 90 postpartum was compared with Chi-square. In a second experiment, the effect of the treatment with GnRH intramuscular (21 μg of Buserelin acetate, Gestar®, OVER, Argentina) on the rate of pregnant primiparous buffaloes for day 90 postpartum was evaluated. For this, 47 primiparous buffaloes from a commercial farm (La Fria, Tachira, Venezuela) calving between October- December, with a BCS of at least 3 (scale of 1 to 5) and without follicular or luteal structures at day 30 postpartum, were divided into three groups: Control (n=11), no treatment; G30 (n=18), treated with 21μg of GnRH at day 30 postpartum; and G45 (n=18), treated with 21 μg of GnRH at day 45 postpartum. The association between the treatment and the proportion of water buffaloes pregnant at day 90 postpartum was determined with the Chi-square test. In both experiments, animals grazed in pastures consisting of creeping river grass (Echinochloa polystachya), tanner (Bachiaria arrecta), and humidicola (Brachiaria humidicola) in one-day paddock rotations. The bull was present continuously with the herd of buffaloes, and the pregnancy diagnosis was carried out by rectal palpation at day 90 postpartum. In experiment 1, the percentage of buffaloes becoming pregnant in the first 90 days postpartum was lower in primiparous (25.97% vs. 43.98%, Chi-square = 6.573, p<0.05). The odds of pregnancy for day 90 in primiparous buffaloes was only 0.4468 (95% CI:0.2571-0.7763) and was statistically significant (p=0.0.0043). In experiment 2, the overall rate of water buffaloes becoming pregnant during the first 90 days postpartum was 59.57% (28/47). In the control group, it was 45.45%, while in the groups treated with GnRH, it was 77.77% for G30 and 50% for G45. The numerical difference in the percentage of pregnant buffaloes between treatment at day 30 versus treatment at day 45 or the control group did not reach statistical significance (p>0.05). Similarly, no significant differences were observed between the control group and the buffaloes treated at day 45 (Chi-square = 0.055, p=0.8152). In a separate experience, on a farm located in Tres Islas, Tachira, Venezuela, 31/34 of primiparous buffaloes treated with 21 μg of GnRH at day 30 postpartum became pregnant (a control group was not included, and therefore no statistical analysis was made). In conclusion, given the lower percentage of primiparous buffaloes becoming pregnant during the first 90 days postpartum, this category must be considered a group with a high risk of reproductive failure, and differential management must be implemented to avoid this situation. Using 21 μg of GnRH at day 30 postpartum resulted only in a numerical increase in pregnancy rate at day 90 postpartum in primiparous buffaloes. Therefore, this treatment could be an economical alternative to improve the reproductive efficiency of primiparous buffaloes calving in the short photoperiod season, managed with natural service, and having a good body condition score. Still, the results of this treatment must be validated with a larger population.

Are the acute phase proteins related to anestrus in primiparous water buffaloes? Preliminary results

Primiparous water buffaloes are a group with more riskfor low reproductive performance, mainly due to a long periodof postpartum anestrus, which could have a multifactorial or-igin. It is unknown if inflammatory diseases may promote thelong anestrus period in primiparous buffaloes. Therefore, this preliminary analysis aimed to compare C reactive protein andfibrinogen levels, two acute phase proteins, and body condi-tion score and cholesterol levels in primiparous water buffaloesduring the early postpartum and after a long anestrus period. Tothis, ten primiparous water buffaloes from a commercial farm inLa Fria, Tachira, Venezuela, were submitted to a reproductiveultrasonographic evaluation; no signs of uterine disease or cor-pora lutea were observed in any buffalo; and therefore, thesewere considered in anestrus and grouped as early postpartum(n=4, mean of postpartum days 15±2.70) or prolonged anestrus(n=6, mean of postpartum days 124.5±36.55, p< 0.05). More-over, the body condition score was evaluated using a 1 to 5scale with 0.25 increments (1 = thin, 5 = fat), and blood sam-ples were collected to measure the levels of C-reactive protein(by ELISA), fibrinogen (by Clauss assay), and cholesterol (byColorimetric method). All the means were compared by T-testusing R studio; additionally, a correlation analysis was carriedout. No differences were observed for any of the studied vari-ables. Body condition score was lower than 3 points in bothgroups (2.31±0.51 and 2.33±0.37 for early and late postpartum,respectively, p=0.947). The cholesterol level was 142.75±9.74mg/dL and 156±12.26 mg/dL, respectively (p=0.133). C-reac-tive protein averaged 60±24 mg/dL and 33±18 mg/dL for earlyand late postpartum buffaloes, respectively (p=0.137). At thesame time, fibrinogen levels were 318.5±161.77 mg/dL forearly postpartum and 318.83±69.78 mg/dL, respectively (p =0.914). C-reactive protein and cholesterol levels were negative-ly correlated (r = -0.71, p=0.048). Because no differences wereobserved in the variables studied in both postpartum moments,it is possible to suggest that a body condition score lower than3.0 and persistent levels of C-reactive protein, fibrinogen, andcholesterol could be related to a prolonged period of anestrusin primiparous water buffaloes. However, given the reducednumber of animals included and the absence of cyclic females,the results reported should be taken cautiously and verified ina larger population.

Evaluation of Parity Effect on Characteristics and Minerals in Buffalo (Bubalus Bubalis) Colostrum and Mature Milk.

Colostrum is a vital performance for buffaloes and potentially functional foods in the future. Therefore, this study aimed to evaluate the difference between the parity of buffalo colostrum and mature milk. Twenty pregnant buffaloes (primiparous = 10; multiparous = 10) were assigned to the same diet prepartum and milking routine postpartum. Calves were separated from the dams immediately after birth and colostrum was harvested within 2 h, whilst mature milk was harvested at 7 days postpartum. The colostrum was analyzed for immunoglobulin G and milk composition as the mature milk. The results showed that there was a higher level of protein, solid not fat, and milk urea nitrogen (p < 0.05), with a tendency for higher total solids (p = 0.08) in primiparous buffaloes' colostrum compared with multiparous. No parity effect was observed in colostrum immunoglobulin G, fat, lactose, and yields of colostrum and composition (p > 0.05). There was no difference in mature milk composition and yield by parity affected (p > 0.05). Compared with mature milk composition, colostrum had a higher content protein, total solids, solid not fat, and milk urea nitrogen (p < 0.05); however, fat and lactose were lower than that of mature milk (p < 0.05). For minerals, multiparous buffaloes' colostrum had a higher concentration of Fe (p = 0.05), while the mature milk had higher concentrations of K and P compared with primiparous. Buffalo colostrum had higher concentrations of Na, Mg, Co, Fe, and K with a lower concentration of Ca relative to mature milk (p < 0.05). It was observed that parity affected colostrum characteristics rather than mature milk and caused subtle variations in minerals in colostrum and mature milk of buffaloes. As lactation proceeded, both milk composition and minerals in the milk changed drastically.

Pregnancies following Protocols for Repetitive Synchronization of Ovulation in Primiparous Buffaloes in Different Seasons.

Primiparous buffaloes were tested in two periods of the year characterized, by either low or high reproductive efficiency. They were subjected to two protocols for synchronization of ovulation: (i) Ovsynch (OV) and (ii) progesterone based (P4) treatment. After calving, the animals underwent a series of four cycles of re-synchronization protocols. The season did not affect pregnancy rates when the results of the two treatments were pooled together with regard to the first synchronization protocol, followed by AI. Pregnancy rates were similar during the low breeding season (50.3% vs. 57.4% in OV and P4, respectively), but different during the high breeding season (50.4% vs. 67.7% in OV and P4, respectively; p = 0.000). Logistic regression confirmed a significant effect of treatment and season interaction on pregnancy (p = 0.003). Following re-synchronization, a treatment by season interaction was detected during the low breeding season (odds ratio = 2.233), in favor of P4. Finally, a survival analysis showed a better response of animals subjected to P4 treatment from the second AI onward. In conclusion, the pooled data of pregnancy rates from both treatments between seasons are not different following AIs. Better results, though, were obtained from the implementation of P4 treatment, and are recorded in a season-fashioned mode when the comparison is made following first or cumulative AIs.

Relationship among production traits, somatic cell score and temperature–humidity index in the Italian Mediterranean Buffalo

The temperature–humidity index (THI) has been commonly used to analyse heat stress in dairy cattle, but little is known about its effects on buffaloes. In this study, daily milk yield (MY), fat percentage (FP), protein percentage (PP) and somatic cell count (SCC) data from 808 buffalo cows plus environmental temperature and relative humidity were used to investigate the consequence of heat stress. Two mixed models were used to evaluate the impact of THI on MY, FP, PP and log transformed SCC (SCS). The effect of THI was significant for PP, FP and SCS, whereas its interaction with parity was statistically significant for PP and SCS. The relationship between PP and FP and THI was positive but of different magnitude according to the parity. When THI was below 62, an unfavourable effect was observed, especially in primiparous buffalo cows. A significant interaction between SCS and THI across parities was also observed. The effect of THI on MY across parities was not definite but overall a favourable relationship was observed. Our findings depict a susceptibility of buffaloes to low values of THI, suggesting an optimal THI range for water buffaloes between 59 and 63, although some deleterious effects were observed in primiparous buffaloes at THI values lower than 62. Additional investigations are needed to better elucidate the influence of THI on buffalo species. HIGHLIGHTS The overall effect of THI on buffalo diverges from what commonly observed in dairy cattle Cold stress affects milk and udder health in buffaloes The effect of THI on buffaloes’ performance depends on parity, with a larger susceptibility in primiparous than pluriparous buffalo cows Udder health in buffaloes, evaluated using somatic cell count, is also affected by THI

Influence of Days after Calving and Thermal Stress on the Efficacy of a Progesterone-Based Treatment in Acyclic Italian Mediterranean Buffalo

Simple Summary“Mozzarella di Bufala Campana DOP” (mozzarella cheese) is mainly produced and marketed during the spring and summer months. The buffalo is a seasonal species that increases its reproductive activity when daylight hours decrease. Therefore, to increase milk production in the favourable period, the so-called “Out of Breeding Season Mating” technique is applied. It consists of the interruption of sexual promiscuity during the naturally occurring breeding season and concentrating calving and milk production during periods of increasing daylight length. However, the application of this technique increases the incidence of anoestrus, as animals are forced to breed outside of their natural and favoured period of the year, although other factors can also increase the incidence of anoestrus, such as climate. A reduction of seasonal anoestrus can be achieved by using some hormonal treatments. In this study, primiparous acyclic buffaloes were selected and divided into three classes according to their days in milk. Animals were synchronized using P4 vaginal implants, and artificial insemination (AI) was performed according to protocol. The temperature–humidity index (THI) was recorded to evaluate its influence on anoestrus. Statistical analysis showed that the implemented P4-based treatments were highly effective in removing the anoestrus condition in buffaloes. On the contrary, no influence of the THI on the efficacy of the P4 synchronization treatment was observed.The aim of this study was to evaluate the efficacy of a progesterone-based treatment on anoestrus in buffaloes. Primiparous acyclic buffaloes (n = 276), were divided into three classes according to their days in milk (DIM): from 50 to 90 (Class I; n = 86), from 91 to 150 (Class II; n = 102) and from 150 to 200 (Class III; n = 88). Animals were synchronized using P4 vaginal implants, followed by timed artificial insemination (TAI). They were then allowed to enter into a larger group of buffaloes for natural mating 15 days after AI was performed, and pregnancy status was monitored from then on at 15-day intervals. Finally, the temperature–humidity index (THI) was calculated. Statistical analysis was performed by ANOVA by means and both multiple and linear regression. The total pregnancy rate (PR) was 87.7%, with no differences among DIM classes (88.0, 92.4, and 80.0% in Classes I, II, and III, respectively). However, the PR at TAI tended to be higher (p = 0.07) in buffaloes in Class II. The follicle (FL) area in Class II buffaloes was larger (p < 0.01) than that of the other classes. No influence of the THI on the total PR was recorded. The pregnancy outcome at TAI was affected by the FL area (odds ratio = 2.237; p < 0.05) and body condition score (BCS) (odds ratio = 1.256; p < 0.05). In conclusion, treatment with vaginal P4 optimizes pregnancy rates in anoestrus buffaloes, particularly when the animals are in mid-lactation and show an optimal BCS. Furthermore, the THI does not seem to affect the efficiency of the progesterone treatment.

Maternal and Neonatal Behaviour in Italian Mediterranean Buffaloes.

Simple SummaryA clear understanding of species-specific natural behaviours is crucial for assessing animal welfare. In this regard, one of the most sensitive and critical moments for dairy farming animals is the post-partum period. Currently, very little information is available on this period in buffaloes. Therefore, the aim of the study was to investigate the maternal and neonatal behavioural patterns of Italian Mediterranean buffaloes during the early post-partum period and the relationship of both dam and calf welfare indicators (e.g., behaviours and growth rate). Behavioural patterns (postural, grooming, maternal rejection, and feeding) are described in detail for both dams and calves. Maternal rejection behaviours (pawing the calf, pushing the calf, and avoidance behaviour) are found to be negatively correlated with calf growth. In this regard, monitoring buffaloes’ maternal behaviours seems to be crucial, especially for primiparous mothers, in order to prevent welfare consequences for newborns when raised together.The aim of this study was to describe the neonatal and maternal behaviour of Italian Mediterranean buffaloes. Thirty primiparous buffaloes were moved into individual pens 12.5 (±2.5) days before calving. Maternal and neonatal behaviours were recorded for 48 h after calving and the analysis was performed in continuous sampling with the software BORIS. Calves’ clinical evaluations (temperature, weight, and heart and respiratory rates) were performed at different time intervals and correlated with behavioural data from the dam. Data were analysed with parametric and non-parametric methods after controlling their distribution. The maternal behavioural pattern found highlighted buffaloes’ priorities during the post-partum period: firstly, they stand and start grooming to ensure proper care for the calf; it is only after this that they dedicate time to maintenance behaviours (feeding and lying). The dams mainly groomed the calf during the first six hours after calving (average time in the 1–6-h interval: 7.7 ± 2.5 min., F = (2.5, 60.2) = 75.0; p < 0.001) to ensure the formation of the mother–infant bond; thereafter, the behaviour decreased over time. As reported in the literature, inexperienced mothers could sometimes delay the calf’s first suckling with aggressive or rejection behaviours. In this regard, 16 buffalo dams showed at least one maternal rejection behaviour, which was found to negatively correlate with calves’ daily weight gain (DWG) at 14 (rs = −0.5, p = 0.02) and 21 days (rs = −0.7, p < 0.001). The calves took on average 212.0 ± 110.0 min to suckle, and this behaviour was mainly shown during the first six hours. Overall, suckling behaviour was correlated with standing: (rs = 0.6, p < 0.001) and walking (rs = 0.9, p < 0.001). The calves’ live weight and DWG were consistently higher than the values reported in the literature. Our results present a detailed description of maternal and neonatal behaviour in the early post-partum period in Italian Mediterranean buffaloes. We also found that maternal rejection behaviours can negatively influence the calves’ growth. Finally, we think that such results can improve the management of buffaloes during the period around parturition.

Improvement of Reproductive Parameters in Postpartum Buffalo Cows

The aim of this study was to evaluate the effect of the hormonal treatment during early postpartum period on the days open, serum progesterone hormone levels at 28 and 42 days post injection and pregnancy percentage following different hormone protocols in multiparous and primiparous buffaloe- cows. The experiment was carried out on 65 (40 multiparous -25 primiparous) clinically healthy animals, randomly allotted into five groups: each (n=8) multiparous and (n=5) of primiparous. The first group; received saline 0.9% intramuscular at day 14 postpartum (control). The second group; injected by 5 ml Receptal (20 µg Buserelin ) at day 14 post-partum. The third group; injected by 5 ml Receptal at day 14 post-partum and day 21 then 3ml Estrumate (750 µg cloprostenol) at day 28. The fourth group received 2 vials of Gonaser (1000 IU eCG) at day 14 post-partum. The fifth group injected by 1000 IU eCG at day 14 post-partum, and at day 21 receive 5 ml Receptal then 7 days later at day 28 receive 3ml Estrumate. Fertile bulls were ensured for all buffaloes. Blood samples collection were performed at the days 28 and 42 day from treatment for serum progesterone levels determination. Ultrasound pregnancy check was done at day 40-45 days later. The present study demonstrated that group 4 has significant (P>0.05) decrease of the current days open than other groups of multiparous buffalo and have lower significant (P < 0.01) difference from previous days open and Primiparous buffaloes. Meanwhile, control group showed the highest current days open (113.3 ± 4.4) compared to other groups of multiparous buffalo cows and have significant (P < 0.05) difference from primiparous buffalo cows. Highly significant (P < 0.05) differences in serum progesterone concentration at 28 and 42 day among groups of multiparous buffalo cows where, group 4 at 28 day had greater (0.56±0.11 ng/mL) concentration than both group 2 (0.31±0.07 ng/mL) and group 3 (0.29±0.06 ng/mL). No significant differences in percentages of pregnancy rates within multiparous and primiparous buffaloes but group 1 and group 3 of primiparous buffaloes had the lowest pregnancy rate percentage compared to other groups. In conclusion, the application of hormonal treatment during early postpartum period at day 14 postpartum especially group 4 resulted in decreased days open and could be used for improvement of the reproduction in postpartum buffaloe-cows.

Milk yield and composition, blood, and urinary parameters of Murrah buffaloes in different maturity stages during the transition period and early lactation

ABSTRACT This study aimed to evaluate differences in productive performance between primiparous and multiparous Murrah buffaloes and the interrelationships between metabolic traits during the transition period and early lactation. Thirty pregnant buffaloes were monitored during the transition period and at the beginning of the lactation. Animals were randomly assigned within the two experimental groups considering the calving number and the estimated calving date: primiparous (n = 15) and multiparous buffaloes (n = 15). The buffaloes were monitored every week during the last 30 days of pregnancy, and the first 63 days postpartum. Buffaloes were kept in the same environment condition, and management practices. Multiparous buffaloes, at the postpartum period, showed higher milk fat, protein, lactose, total dry extract production, non-fat dry extract contents, and higher milk urea nitrogen and casein contents than primiparous buffaloes. Primiparous buffaloes showed higher urine pH and hematocrit concentration than the multiparous group at the prepartum period and higher leukocyte and lymphocytes concentrations at the postpartum. During the transition period, primiparous buffaloes exhibited negative interrelationships between metabolic traits and productive performance related to variations in their metabolic status. These results may indicate that multiparous buffaloes fewer sensitive to variations of metabolic status during the transition period.

Factors affecting calving ease in Egyptian buffalo.

Calving ease (CE) is a trait of economic importance that affects animal welfare and farm profitability. The objective of present study was to investigate genetic and environmental factors affecting CE among Primiparous (PP) and multiparous (MP) buffaloes. A total of 9,627 records from 1999 MP and 2,110 PP recorded during the period from 1988 to 2018 were considered. Herd, season of calving, year of calving, birth weight, parity order and gestation length significantly affected CE rate, while age at first calving and sex of calf had no significant effects. Direct and maternal heritabilities of CE in PP and MP were 0.06 and 0.01, respectively. The low heritability of CE indicated that direct selection may not be an effective method to improve CE trait in Egyptian buffalo.

Effects of Chickpea in Substitution of Soybean Meal on Milk Production, Blood Profile and Reproductive Response of Primiparous Buffaloes in Early Lactation.

Simple SummaryCurrently, the protein needs of lactating buffaloes are mainly covered by soybean derivatives produced predominantly overseas. In order to promote the use of locally produced protein sources, in this study we tested the effects of total replacement of soybean meal by using chickpea meal, a protein-rich legume well adapted to and traditionally grown in the Mediterranean area. We evaluated the effects of these two alternative protein sources on blood profile, reproductive response and milk traits in primiparous buffaloes in early lactation. Based on our findings, chickpea meal does not impair the productive and reproductive performances of primiparous dairy buffaloes. In addition, chickpeas may represent a good alternative protein source for organic farms as it is not at risk of contamination by genetically modified cultivars.This study aimed to evaluate the effects of the use of chickpea meal in substitution of soybean meal on plasma metabolites, reproductive response, milk yield and composition and milk coagulation traits of primiparous buffaloes in early lactation. Eighteen primiparous buffaloes were blocked by age, body weight and days in milk and equally allotted to two experimental groups from 10 to 100 days of lactation. The experimental diets consisted of the same forage integrated with two different isonitrogenous and isoenergetic concentrates containing either 210 g/kg of soybean meal or 371 g/kg chickpea. The use of chickpea meal had no negative effects on dry matter intake (p = 0.69), body condition score (p = 0.33) and milk yield (p = 0.15). Neither milk composition nor blood metabolites were influenced by dietary treatments (p > 0.05), but an increment of urea concentrations in milk (p < 0.05) and blood plasma (p < 0.001) were observed in buffaloes fed chickpeas. Moreover, no effect (p > 0.05) of the dietary treatment was highlighted on milk coagulation traits as well as buffalo reproductive responses. We concluded that soybean meal can be replaced by chickpea meal in the diet for primiparous dairy buffaloes in the early lactation period without impairing their productive and reproductive performance.

Phenotypic Characterization of Milk Yield and Quality Traits in a Large Population of Water Buffaloes.

Simple SummaryThe buffalo dairy industry has deep roots in Southern Italy, due to the traditional link with Mozzarella di Bufala, a Protected Designation of Origin cheese with high economic value and market demand. At farm level, strategies aiming to improve milk yield and quality are essential to maximize profitability and dairy chain efficiency. In this study, we analyzed a large data set of Italian Buffaloes (around 70 thousands animals) in order to detect the phenotypic sources of variation of milk yield and quality traits, disclose exploitable favorable correlations among milk traits, and provide useful information for dairy buffalo chain stakeholders.The buffalo milk industry has economic and social relevance in Italy, as linked to the manufacture of traditional dairy products. To provide an overview of the current status of buffaloes’ performances on a large scale, almost 1 million milk test-day records from 72,294 buffaloes were available to investigate milk yield, energy corrected milk, fat, protein, and lactose content, and somatic cell score (SCS). Phenotypic correlations between milk traits were calculated and analysis of variance was carried out through a mixed model approach including fixed effect of parity, stage of lactation, sampling time, month of calving, and all their interactions and random effects of buffalo, herd-test-date, and residual. Third-parity buffaloes were the most productive in terms of milk yield, while the lowest solid content was detected in sixth parity buffaloes. A considerable gap between primiparous and multiparous buffaloes was observed for milk yield, especially in early- and mid-lactation. Overall, SCS progressively increased with parity and showed a negative correlation with milk yield in both primiparous (−0.12) and multiparous (−0.14) buffaloes. Results suggested that, at the industrial level, milk of primiparous buffaloes may be preferred for transformation purposes, since it was characterized by greater solid content and lower SCS. Results of this study provide a picture of the Italian population of buffaloes under systematic performance records and might be beneficial to both dairy industry and breeding organizations.

Associations between MUC1 gene polymorphism and resistance to mastitis, milk production and fertility traits in Murrah water buffaloes

ABSTRACT MUC1 is a glycoprotein mucin expressed in apical cells of luminal tissues, such as mammary gland luminal cells, which the main function is protect the cell surface from the environment microorganisms. The aim of this study were to identify the polymorphism of MUC1 gene in Murrah breed and to analyze the association between molecular marker and somatic cell counting, milk yield, fat yield, fat percentage, protein yield, protein percentage, age at first calving and calving interval. A total of 179 primiparous Murrah buffaloes were genotyped by PCR technique. Milk-recording were performed periodically to determine fat and protein content and somatic cell count. Three alleles of different lengths were amplified and named allele 1, 2 and 3, respectively. The allele 2 was more frequent followed by allele 3. The MUC1 gene was highly associated with traits related to mastitis resistance and milk percentage. Also was demonstrated a mild association between MUC1 gene and milk protein synthesis and a moderate effect on reproductive trait. The MUC1 gene is polymorphic in water buffaloes of Murrah breed and might be a potential marker to marker-assisted selection for intramammary infection and some milk production traits.

Serum biochemical profile of neonatal buffalo calves

ABSTRACT Serum blood samples from 50 Murrah buffalo calves were examined in this study. The animals were allocated into three groups according to the number of parturitions of their mothers: G1 (n= 15) calves from primiparous buffaloes, G2 (n= 19) calves from buffaloes with two to four parturitions, and G3 (n= 16) calves from buffaloes with five or more parturitions. Blood samples were taken at birth, before colostrum ingestion, at 24h, 48h, and 72h after birth, and at 7, 14, 21, and 30 days after birth for determination of levels of gammaglutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatine kinase, total protein, albumin, globulins (including immunoglobulin G), iron, total calcium, ionized calcium, phosphorus, sodium, and potassium. The age of the calves was found to influence all of the biochemical parameters, with the exception of ionized calcium and potassium in the calves in groups G1 and G3. The calving order was found to influence AST, GGT, total protein, albumin, and globulins, including IgG. The high serum ALP activity in the first two days after birth indicates that measurement of the levels of this enzyme may be used as an indirect method of assessing passive immunity transfer.

Peas may be a candidate crop for integrating silvoarable systems and dairy buffalo farming in southern Italy

Integration of trees with agricultural crops and livestock systems may offer advantages in term of productivity, economic return, and sustainability. In order to identify a candidate crop for Mediterranean silvoarable systems to be used in livestock farming as a protein source locally produced, a study was undertaken to evaluate the effect of feeding peas (Pisum sativum L.) as main protein source on milk yield and in vivo digestibility of primiparous buffaloes. Two almost isonitrogenous concentrates (on average, crude protein 240 g/kg dry matter) were formulated to contain, as fed basis, either 350 g/kg of soybean cake (SoyC) or 450 g/kg of extruded peas (PeaC) as the main protein source. Twenty primiparous buffaloes were blocked by age and body weight into two dietary treatments (Soyand Pea) from 10 to 100 day in milk. All cows were fed in the barn a total mixed ration containing 3 kg of SoyC and in the milking parlour they were individually supplemented by either 3 kg of SoyC or PeaC according to the group assignment. The substitution rate of soybean protein by pea protein was approximately 30%. Milk yield was not affected by the dietary use of extruded peas, as well as, milk fat and protein percentages, and clotting properties. Moreover, in vivo digestibility did not differ between the two dietary groups. Results support the partial substitution of soybean cake with extruded peas in diets for lactating buffaloes.

Effects of an automatic milking system on milk yield and quality of Mediterranean buffaloes

A study was carried out to evaluate the effects of an automatic milking system (AMS) on milk yield and composition of buffalo (Mediterranean-type Bubalus bubalis) cows. Performed from January 2015 to December 2015 in an organic buffalo dairy farm equipped with both a traditional tandem milking parlor and an AMS, the study involved 90 primiparous buffaloes randomly allotted to a tandem or AMS group from 5 to 10 d of lactation onward. Number of milkings per day and daily milk yield of each cow were recorded, and individual milk sampling was carried out twice a month. Compared with the tandem, the AMS group showed significantly higher daily milk yield and persistence of lactation. Use of the AMS resulted in higher protein and casein contents, and lower somatic cell and total bacterial counts, whereas fat, freezing point, and pH were unaffected by the system. We conclude that, in terms of milk yield and quality, automatic milking may be a suitable alternative to conventional milking for buffaloes.

Survival analysis and seasonal patterns of pregnancy outcomes in Egyptian buffaloes

The objective of this study was to analyze the survival patterns of pregnancy outcomes in Egyptian buffaloes under subtropical environmental conditions. The overall reproductive and production indices were performed on a total of 1224 records. The means of days at first AI postpartum, days open and calving interval of Egyptian buffaloes are 62.29, 83.13 and 395.1 days, respectively. The survival analysis of pregnancy outcomes in the present study was restricted to the first AI (576 first AI records). Compared to the low temperature-humidity index (THI), the conception rate (P/AI at 30 days) was significantly decreased at high THI level (OR = 0.45; p = 0.029), however, no significant variations were recorded between the referent (low) THI level and the moderate one (OR = 0.86; p = 0.673). Additionally, the pregnancy loss rate was significantly increased at a high THI level (OR = 2.43; p = 0.008). In comparison with the referent (primiparous) group, the conception rates were significantly increased in the M1 (2–5 parities; OR = 2.84) and M2 (>5 parities; OR = 4.06) multiparous groups (p = 0.007 and 0.004, respectively). The Egyptian buffaloes produce a relatively higher daily milk yield (>9 kg) have significantly lower conception rate compared with the referent (<7 kg) group (OR = 0.46; p = 0.046). However, the daylight length (continuous variable) had no significant effects on the conception (OR = 0.88; p = 0.298) and pregnancy loss (OR = 0.92; p = 0.608) rates. The Cox Proportional-hazards model for the hazard of pregnancy outcome (P/AI at 30 days) revealed that multiparous buffaloes had a higher hazard of pregnancy than primiparous buffaloes (HR = 2.37, p = 0.001). Moreover, buffalo cows calving in the summer and spring have a lower hazard of pregnancy than buffaloes calve in the winter season (HR = 0.57 and 0.76, p = 0.001 and 0.002, respectively). The current study indicates that Egyptian buffaloes have the ability to tolerate the thermal stress up to 80 THI values without deterioration of pregnancy outcomes. Moreover, primiparous or high producing buffalo cows may require more effective reproductive strategies as modulation of breeding plans to get calving in the winter and autumn seasons.

Risk factors and genetic evaluation of stillbirth trait in buffalo

Abstract Stillbirth is a trait with negative effects on farm profitability and animal welfare. The objectives were to investigate risk factors for stillbirth in Egyptian buffaloes, and to estimate variance components using Bayesian threshold model. The data consisted of 7985 records for 1764 multiparous and 1752 records for 896 primiparous buffaloes born between 1980 and 2013. Risk factors were studied using GENMOD procedure of SAS, and genetic parameters were estimated using Bayesian threshold model. Year of calving, herd, parity, birth weight and gestation length effected significantly on stillbirth rate, but season of calving and age at first calving had no significant effect on stillbirth rate. The direct heritability for primiparous and multiparous were 0.03 and 0.02, respectively, the maternal heritability was 0.06 for primiparous and multiparous, and the repeatability was 0.114. The low heritability of stillbirth indicated that genetic improvement would not be effective tool of improving stillbirth trait in Egyptian buffaloes.

Response of primiparous and multiparous buffaloes to yeast culture supplementation during early and mid-lactation.

Strains of live Saccharomyces cerevisiae yeast have exhibited probiotic effects in ruminants. This study investigated the effects of the dietary yeast supplement, S. cerevisiae (Yea-Sacc1026), on primiparous (PP) and multiparous (MP) Egyptian buffaloes in early to mid-lactation. Lactating buffaloes were fed either a basal total mixed ration (TMR, control; 4 PP and 8 MP) or the basal TMR plus 10 g Yea-Sacc1026 per buffalo cow per day (yeast; 4 PP and 8 MP). The feeds were given from 15 days prepartum to 180 days postpartum. Feed intake, body weight, and milk yields (MY) were recorded, and milk and blood samples were collected for analyses. Feces were collected from days 45 to 47 during early lactation and from days 90 to 92 during mid-lactation to determine apparent digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Energy corrected milk yield (ECM), feed conversion, and energy and nitrogen conversion efficiency were calculated. Yeast treated MP buffaloes consumed more DM (P ≤ 0.041) and CP than the untreated control group. Apparent digestibility of DM and OM were significantly greater at mid-lactation for treated versus control group (P = 0.001). Crude fiber digestibility was greater in MP than in PP buffaloes (P = 0.049), and yeast supplemented MP cows had a greater CF digestibility than control MP buffaloes at mid-lactation (P = 0.010). Total blood lipids decreased after yeast supplementation (P = 0.029). Milk yields, ECM, fat and protein yields increased for yeast treated MP buffaloes (P ≤ 0.039). The study concluded that the response to yeast supplementation in buffalo cows is parity dependent. Multiparous buffaloes respond to yeast supplementation with an increased DM intake and CF digestibility without significant weight gains, allowing a greater ECM yield with less fat mobilization. Supplementing buffaloes with yeast culture may increase milk production in early lactation and results in a more persistent milk production during mid-lactation. Feed conversion and energy and nitrogen conversion efficiency may be increased with the use of yeast supplementation in Egyptian buffaloes.

Diurnal variation of milk somatic and differential leukocyte counts of Murrah buffaloes as influenced by different milk fractions, seasons and parities

Milk cell counts are good indicators of a mammary infection and milk quality. The present study was done to record diurnal rhythmicity in the milk somatic and differential cell counts during different seasons, milk strips and parity in Murrah buffaloes. Milk somatic cell counts (SCC) were measured by SCC counter and milk differential cell counts were measured microscopically after making a milk smear and staining it to identify neutrophils, lymphocytes and macrophages. Maximum milk SCC was observed in the summer season. Milk neutrophils were lowest during thermoneutral (TN), intermediate during the winter season and highest during the summer season. Milk lymphocytes were highest during the winter season, intermediate in the TN and lowest in the summer season. Diurnal rhythm in the milk SCC and neutrophil percentage is noticed in the summer season only. Maximum milk SCC values were observed in the late strip but neutrophils were highest in the early strip. Diurnal rhythm was observed in the late strip for neutrophils and in mid strip for the lymphocytes. Milk SCC and milk neutrophils were found to be the highest in the multiparous buffaloes and diurnal rhythm was observed only in the lymphocytes of primiparous buffaloes. Milk macrophages were higher in the morning samples of primiparous as compared to the multiparous buffaloes. In this pioneer study, diurnal rhythms in the milk cell counts of buffaloes have been studied extensively. This will help in maintaining low milk cell counts in buffalo and thus help in getting more milk per buffalo during stress periods.