Priming Experiments Research Articles

The head or the verb: Is the lexical boost restricted to the head verb?

Four structural priming experiments investigated whether the lexical boost is due to the repeated head verb of the primed structure or due to the repetition of any verb, testing structural priming of ditransitive structures (The hotel owner decided to loan the tourist a tent/a tent to the tourist). In Experiments 1–3, we manipulated the repetition of the matrix verb (decided) that is not the syntactic head in the primed structure. The results showed abstract structural priming of the embedded ditransitive structure but the repetition of the matrix verb did not boost the priming. In addition to manipulating the repetition of the matrix verb, we also manipulated the head verb of the primed structure (loan) in Experiment 4. It showed a lexical boost with the repetition of the head verb but no boost with the repetition of the matrix verb. These results are consistent with the residual activation model, which only predicts a boost from the verb that is the head of the primed structure. They do not support models which predict that the repetition of any lexical material in a sentence boosts priming.

Limited Potential for Mineralization of Permafrost Peatland Soil Carbon Following Thermokarst: Evidence From Anoxic Incubation and Priming Experiments

AbstractPermafrost thaw in peatlands risks emitting vast stores of soil organic carbon (SOC) as greenhouse gases to the atmosphere, yet anoxic conditions and low peat quality may prevent rapid SOC loss. To assess differences in anaerobic SOC mineralization following thaw and vulnerability of previously‐frozen peat, we incubated peat (5° and 14°C) from 15 depths of ⁓6 m cores from different thaw sites including an intact permafrost peat plateau and thermokarst bogs that thawed ∼30 and ∼200 years ago. Furthermore, a glucose‐addition experiment after >700 days assessed whether labile C inputs occurring following thermokarst could accelerate SOC mineralization (priming). We found a common pattern of SOC mineralization rate decreasing with depth and peat age. However, we found no differences in peat mineralization rates among sites for samples of similar age and of corresponding peatland developmental stage. Priming effects were minor and short‐term, and did not vary among sites or with peat developmental stage. We also found mineralization rates from anoxic incubations more than an order of magnitude higher than rates implied from field observations, suggesting mineralization rates from anaerobic incubations should be used mainly to interpret relative differences. Peat humification and peat nitrogen and phosphorous content explained more than 85% of the variability in mineralization rates. Peat quality did not influence the temperature sensitivity of peat mineralization. Overall, the absence of substantial priming effects and the lack of differences in mineralization rates between permafrost peat and peat thawed 200 years ago suggests that rapid peatland SOC loss following thaw is unlikely.

Grammatical encoding of double-object alternations in Zhuang: Evidence for the one-stage model.

Production research has yielded mixed findings regarding whether grammatical encoding specifies grammatical functions and linear word order simultaneously or separately, supporting either one-stage or two-stage models. Here, we focused on the double object (DO) and shifted double object (SDO) constructions in Zhuang, an ethnic minority language in China because they differ only in linear ordering of the two nouns whose grammatical functions are direct object and indirect object, assuming the roles of Theme and Recipient, respectively. Using two structural priming experiments, we found that both DO and SDO constructions induced within-structure priming effects, but they did not prime each other. Such structural priming effects persisted, regardless of whether semantic features (i.e., animacy of the Theme) were repeated across primes and targets. Taken together, these priming patterns support the one-stage model of grammatical encoding, where a conceptual representation is converted into a structure specifying both grammatical functions and linear word order.

"Ladetes"-A novel device to test deformation behaviors of hydrate-bearing sediments.

Natural gas hydrate (NGH) exploitation is severely restricted by geotechnical problems. Deformation behaviors of the hydrate-bearing strata (HBS) control the occurrence and evolution of geotechnical problems during extracting natural gas from HBS. In this paper, a novel approach named Ladetes is introduced to evaluate the lateral deformation behaviors of the near-wellbore and fracture-filling regions of the HBS. The pressuremeter test and the flat dilatometer test are designed to simulate the inner boundaries of an NGH-producing well and an artificial stimulation fracture for the first time. The device can realize the in situ hydrate formation prior to the experiment and axial loading application throughout the experiment. Both the strain control mode and the stress control mode can be achieved to estimate the deformation characteristics of HBS under different downhole conditions. Prime experiments proved their adaptability and reliability. The Ladetes provides an effective and alternative way of obtaining geotechnical parameters for HBS.

Biases in BCI experiments: Do we really need to balance stimulus properties across categories?

Brain Computer Interfaces (BCIs) consist of an interaction between humans and computers with a specific mean of communication, such as voice, gestures, or even brain signals that are usually recorded by an Electroencephalogram (EEG). To ensure an optimal interaction, the BCI algorithm typically involves the classification of the input signals into predefined task-specific categories. However, a recurrent problem is that the classifier can easily be biased by uncontrolled experimental conditions, namely covariates, that are unbalanced across the categories. This issue led to the current solution of forcing the balance of these covariates across the different categories which is time consuming and drastically decreases the dataset diversity. The purpose of this research is to evaluate the need for this forced balance in BCI experiments involving EEG data. A typical design of neural BCIs involves repeated experimental trials using visual stimuli to trigger the so-called Event-Related Potential (ERP). The classifier is expected to learn spatio-temporal patterns specific to categories rather than patterns related to uncontrolled stimulus properties, such as psycho-linguistic variables (e.g., phoneme number, familiarity, and age of acquisition) and image properties (e.g., contrast, compactness, and homogeneity). The challenges are then to know how biased the decision is, which features affect the classification the most, which part of the signal is impacted, and what is the probability to perform neural categorization per se. To address these problems, this research has two main objectives: (1) modeling and quantifying the covariate effects to identify spatio-temporal regions of the EEG allowing maximal classification performance while minimizing the biasing effect, and (2) evaluating the need to balance the covariates across categories when studying brain mechanisms. To solve the modeling problem, we propose using a linear parametric analysis applied to some observable and commonly studied covariates to them. The biasing effect is quantified by comparing the regions highly influenced by the covariates with the regions of high categorical contrast, i.e., parts of the ERP allowing a reliable classification. The need to balance the stimulus's inner properties across categories is evaluated by assessing the separability between category-related and covariate-related evoked responses. The procedure is applied to a visual priming experiment where the images represent items belonging to living or non-living entities. The observed covariates are the commonly controlled psycho-linguistic variables and some visual features of the images. As a result, we identified that the category of the stimulus mostly affects the late evoked response. The covariates, when not modeled, have a biasing effect on the classification, essentially in the early evoked response. This effect increases with the diversity of the dataset and the complexity of the algorithm used. As the effects of both psycho-linguistic variables and image features appear outside of the spatio-temporal regions of significant categorical contrast, the proper selection of the region of interest makes the classification reliable. Having proved that the covariate effects can be separated from the categorical effect, our framework can be further used to isolate the category-dependent evoked response from the rest of the EEG to study neural processes involved when seeing living vs. non-living entities.

Expertise-dependent perceptual performance in chess tasks with varying complexity.

Perceptual performance, anticipating opponents' strategies, and judging chess positions especially in subliminal processing is related to expertise level and dependent on chunking processes. It becomes obvious that chess expertise is a multidimensional phenomenon related predominantly to experience. Under consideration of chess expertise categorization, we conducted two priming experiments expanding existing designs by gradually increasing the target and task complexity. The main aim was the evaluation of potential visuocognitive limitations. The results reveal experts' perceptual superiority manifested by their faster reaction times in settings with increased stimulus and task complexity. Further, experts' priming effects seem to be affected by the target content and/ or priming duration. For short prime duration, experts show priming effects only for less complex prime-target content. Interestingly, for longer prime duration and more complex prime-target content, all participants reveal priming effects. In summary, we argue that experts' visuocognitive processing (i.e., detecting or anticipating potential threats to the king) is rooted in a more efficient visuocognition due to stored chunks of checking and mating constellations. We suggest that visuocognitive limitations are related also to the prime-target complexity as well as to the task. Further investigations must be conducted in order to elucidate the factors with an increased impact on chess players' performance.

“It’s like Hawai’i”: Making a tourist utopia in Jeju Island, 1963-1985

This paper examines the trajectory, ambitions, and practices involved in the official national and provincial planning for Jeju Island from 1963 to 1985 as it became reimagined as the so-called ‘Hawai'i of East Asia’. Jeju Island has been constantly built, left unfinished, demolished, and rebuilt at each wave and ebb in regional tourism trends. Jeju has thus become a complicated geography of heavy contradictions as South Korea’s prime tourism experiment. Before the 2002 ‘Free International City’ project, the larger region of Jeju Island was identified as a ‘specified region’ from 1963 for experimentation in tourism. By virtue of its historic marginality, Jeju has been portrayed as a pristine internal frontier ripe for tourism and utopian transformation ‘like Hawai’i’. Surprisingly, however, ‘Hawai’i’ does not actually appear in official planning documentation, even while it is a frequent talking point in public discourse. In this paper, I discuss the specter of ‘Hawai’i’ in Jeju tourism development and address the discrepancy between official development planning strategies and colloquial references to Hawai’i, observing that reference to ‘Hawai’i’ was not from initial design but followed the late 1950s to 1960s zeitgeist in which tourism itself became a mark of distinction for modernity.

Learning about the meanings of ambiguous words: evidence from a word-meaning priming paradigm with short narratives.

Fluent language comprehension requires people to rapidly activate and integrate context-appropriate word meanings. This process is challenging for meanings of ambiguous words that are comparatively lower in frequency (e.g., the “bird” meaning of “crane”). Priming experiments have shown that recent experience makes such subordinate (less frequent) word meanings more readily available at the next encounter. These experiments used lists of unconnected sentences in which each ambiguity was disambiguated locally by neighbouring words. In natural language, however, disambiguation may occur via more distant contextual cues, embedded in longer, connected communicative contexts. In the present experiment, participants (N = 51) listened to 3-sentence narratives that ended in an ambiguous prime. Cues to disambiguation were relatively distant from the prime; the first sentence of each narrative established a situational context congruent with the subordinate meaning of the prime, but the remainder of the narrative did not provide disambiguating information. Following a short delay, primed subordinate meanings were more readily available (compared with an unprimed control), as assessed by responses in a word association task related to the primed meaning. This work confirms that listeners reliably disambiguate spoken ambiguous words on the basis of cues from wider narrative contexts, and that they retain information about the outcome of these disambiguation processes to inform subsequent encounters of the same word form.

Discrimination, Inclusion, and Anti‐System Attitudes among Muslims in Germany

AbstractMuslims in Europe and North America face high rates of discrimination and hostility. Less clear are the consequences of this prejudice on Muslims’ political attitudes. Leveraging a survey of 1,330 Muslims in Germany, we show that Muslims who have personally experienced discrimination exhibit higher anti‐system tendencies: more supportive of violence, more supportive of Islamism, and less supportive of democracy and secularism. We also find that these patterns are concentrated among Muslims who believe they “suffer alone,” not believing other Muslims experience similar hostility. Finally, through a priming experiment, we find causal evidence that German Chancellor Angela Merkel's inclusive rhetoric and policies toward Muslims may help mitigate these dynamics, reducing perceptions of discrimination and in turn producing pro‐system sentiments.

Orthographic and semantic priming effects in neighbour cognates: Experiments and simulations

AbstractTo investigate how orthography and semantics interact during bilingual visual word recognition, Dutch–English bilinguals made lexical decisions in two masked priming experiments. Dutch primes and English targets were presented that were either neighbour cognates (boek – BOOK), noncognate translations (kooi – CAGE), orthographically related neighbours (neus – NEWS), or unrelated words (huid - COAT). Prime durations of 50 ms (Experiment 1) and 83 ms (Experiment 2) led to similar result patterns. Both experiments reported a large cognate facilitation effect, a smaller facilitatory noncognate translation effect, and the absence of inhibitory orthographic neighbour effects. These results indicate that cognate facilitation is in large part due to orthographic-semantic resonance. Priming results for each condition were simulated well (all r's >.50) by Multilink+, a recent computational model for word retrieval. Limitations to the role of lateral inhibition in bilingual word recognition are discussed.

Guest Editors’ Introduction: Opening Conversations that Matter in Public Music Studies

Guest Editors’ Introduction: Opening Conversations that Matter in Public Music Studies

Visual word recognition among oldest old people: The effect of age and cognitive load.

During the fourth age, a marked physiological deterioration and critical points of dysfunction are observed, during which cognitive performance exhibits a marked decline in certain skills (fluid intelligence) but good performance of others (crystallized intelligence). Experimental evidence describes important constraints on word production during old age, accompanied by a relative stabilization of speech comprehension. However, cognitive changes associated with advanced aging could also affect comprehension, particularly word recognition. The present study examines how the visual recognition of words is affected during the fourth age when tasks involving different cognitive loads are applied. Through linear regression models, performance was compared between two third-age groups and a fourth-age group on reaction time (RT) and accuracy in naming, priming and lexical decision experiments. The fourth-age group showed a significant RT increase in all experiments. In contrast, accuracy was good when the task involved a low cognitive demand (Experiments 1 and 2); however, when a decisional cognitive factor was included (Experiment 3), the fourth-age group performed significantly worse than the younger third-age group. We argue that the behavior observed among fourth-age individuals is consistent with an unbalanced cognitive configuration, in which the fluid intelligence deficit significantly reduces the speed necessary to recognize words, independent of the cognitive load associated with the test. In contrast, the maintenance in crystallized intelligence improves the accuracy of the process, strengthening linguistic functionality in the advanced stages of old age.

Phonetic and Lexical Encoding of Tone in Cantonese Heritage Speakers.

Heritage speakers contend with at least two languages: the less dominant first language (L1), that is, the heritage language, and the more dominant second language (L2). In some cases, their L1 and L2 bear striking phonological differences. In the current study, we investigate Toronto-born Cantonese heritage speakers and their maintenance of Cantonese lexical tone, a linguistic feature that is absent from English, the more dominant L2. Across two experiments, Cantonese heritage speakers were tested on their phonetic/phonological and lexical encoding of tone in Cantonese. Experiment 1 was an AX discrimination task with varying inter-stimulus intervals (ISIs), which revealed that heritage speakers discriminated tone pairs with disparate pitch contours better than those with shared pitch contours. Experiment 2 was a medium-term repetition priming experiment, designed to extend the findings of Experiment 1 by examining tone representations at the lexical level. We observed a positive correlation between English dominance and priming in tone minimal pairs that shared contours. Thus, while increased English dominance does not affect heritage speakers' phonological-level representations, tasks that require lexical access suggest that heritage Cantonese speakers may not robustly and fully distinctively encode Cantonese tone in lexical memory.

Comparative Assessment of Different Pre-Treatment Bonding Strategies to Improve the Adhesion of Self-Adhesive Composites to Dentin.

The aim of this study is to compare the adhesive interface formed in dentin, using self-adhesive composites applied with different bonding strategies, by testing the microtensile bond strength (μTBS) and ultramorphology through the use of light microscopy. Permanent, sound human molars were randomly allocated to six experimental groups. The groups included a negative control group, where only etching was performed via EtchOnly; a positive control group where an adhesive was applied, OptiBondFL (OBFL); and an experimental group where a primer was applied using a co-curing strategy together with a composite (Primer_CoCuring). The samples were sectioned into microspecimens for μTBS (n = 8) and into 1-mm thick slabs for light microscopy using Masson’s trichrome staining protocol (n = 3). The statistical analysis included a two-way ANOVA for μTBS data and Tukey’s HSD was used as a post-hoc test (significance level of 5%; SPSS v. 26.0). The results of the μTBS revealed that the self-adhesive composite (F = 6.0, p < 0.018) and the bonding strategy (F = 444.1, p < 0.001) significantly affected the bond strength to dentin. However, their interactions were not significant (F = 1.2, p = 0.29). Etching dentin with no additional treatment revealed the lowest μTBS (VF_EtchOnly = 2.4 ± 0.8 MPa; CC_EtchOnly = 2.0 ± 0.4 MPa), which was significantly different from using a primer (VF_CoCu = 8.8 ± 0.8 MPa; CC_CoCu = 6.3 ± 1.0 MPa) or using the full adhesive (VF_OptiBondFL = 22.4 ± 0.3 MPa; CC_OptibondFL = 21.2 ± 0.4 MPa). Microscopy images revealed that the experimental Primer_CoCuring was the only group with no collagen fibers exposed to the dentin–composite interface. Overall, the use of a primer, within the limitations of this study, increased the bonding of the self-adhesive composite and provided sufficient infiltration of the collagen based on light-microscopic imaging.

Where is mirror invariance? Masked priming effects by mirrored and rotated transformations of reversible and nonreversible letters

The cognitive mechanism underpinning mirror priming effects for letters (e.g., IDEA primed by ibea) is still unclear: it could be about mirror invariance (perceptual equivalence of mirror images - d and b - which does not extend to other orientation contrasts like plane rotations - d and p) or about visual similarity (b, d, and p share the same visual features and shape). To enlighten it and investigate the modulatory role of task and orthographic context (words vs single letters), we manipulated letter (nonreversible; reversible) and prime condition (identity, mirrored, rotated, control) in four masked priming experiments: lexical decision with conventional and sandwich paradigms (Experiments 1 and 2), same-different task on words and single letters (Experiments 3 and 4). All experiments replicated the differential mirror priming effect: inhibitory for reversible letters, facilitatory for nonreversible letters. Notably, similar priming effects were found for plane rotations. Regardless of task or orthographic context, a reliably larger orientation cost was found for reversible than nonreversible letters for both mirror images and plane rotations. These results suggest that the underlying mechanism is not mirror invariance, but rather visual similarity allied with prelexical dynamics.

Effectiveness of experimental adhesive systems containing carbodiimide and solvated in different solvents

ObjectivesTo evaluate the influence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide – EDC (C) on the degree of conversion (DC%), resin-dentin bonding stability, collagen degradation, and MMP inactivation produced by experimental adhesive systems solvated in acetone (A), ethanol (E) or tetrahydrofuran (T). MethodsEDC was added to experimental primers consisting of HEMA, PMGDM, and water, solvated in A, E, or T, performing six groups (A, AC, E, EC, T, TC). The adhesive consisted of BISGMA, HEMA, camphorquinone, and EDMAB. DC% was evaluated by FT-IR spectroscopy. The μTBS was evaluated after 24 h or 1 yr of artificial saliva storage at 37°C. The degradation of the dentin collagen was evaluated using FT-IR spectroscopy at three periods: after primer application, 15, and 30 days, and the enzymatic activity within the hybrid layer using in situ zymography. Data was analyzed using ANOVA and Tukey's HSD test (α = 0.05). ResultsNo statistical significance in DC% was found among all adhesive systems (p > 0.05). EDC-containing primers maintained μTBS stability after 1 yr of artificial saliva storage (p > 0.05). The intensity of the Amide I and II bands from the dentin collagen was preserved for AC and EC after immersion in collagenase solution. The dentin treated with EDC-containing primers presented reduced fluorescein in the hybrid layer. ConclusionsThe incorporation of EDC into primers contributed to resin-dentin bonding stability and reduced the enzymatic activity and collagen degradation.

Designing and executing prime editing experiments in mammalian cells

Designing and executing prime editing experiments in mammalian cells

Designing and executing prime editing experiments in mammalian cells.

Prime editing (PE) is a precision gene editing technology that enables the programmable installation of substitutions, insertions and deletions in cells and animals without requiring double-strand DNA breaks (DSBs). The mechanism of PE makes it less dependent on cellular replication and endogenous DNA repair than homology-directed repair-based approaches, and its ability to precisely install edits without creating DSBs minimizes indels and other undesired outcomes. The capabilities of PE have also expanded since its original publication. Enhanced PE systems, PE4 and PE5, manipulate DNA repair pathways to increase PE efficiency and reduce indels. Other advances that improve PE efficiency include engineered pegRNAs (epegRNAs), which include a structured RNA motif to stabilize and protect pegRNA 3' ends, and the PEmax architecture, which improves editor expression and nuclear localization. New applications such as twin PE (twinPE) can precisely insert or delete hundreds of base pairs of DNA and can be used in tandem with recombinases to achieve gene-sized (&gt;5 kb) insertions and inversions. Achieving optimal PE requires careful experimental design, and the large number of parameters that influence PE outcomes can be daunting. This protocol describes current best practices for conducting PE and twinPE experiments and describes the design and optimization of pegRNAs. We also offer guidelines for how to select the proper PE system (PE1 to PE5 and twinPE) for a given application. Finally, we provide detailed instructions on how to perform PE in mammalian cells. Compared with other procedures for editing human cells, PE offers greater precision and versatility, and can be completed within 2-4 weeks.

The relationship between verb meaning and argument realization: What we learn from the processing of agent-implying intransitive verbs in Japanese.

This study investigated whether some Japanese intransitive verbs, called agent-implying intransitive verbs, are processed differently from other ordinary intransitive verbs. These verbs are special in that they denote agentive events, but they are intransitive verbs, which only allow the patient/theme to be the only nominatively marked argument. The priming experiment was designed based on the situation model theory, assuming that verbs with an agentive semantic structure (e.g., ordinary transitive verbs) has a shorter causal inferential distance than those with a non-agentive semantic structure (e.g., ordinary intransitive verb). In the experiment, participants were instructed to read two sentences that formed a story, of which the second sentence was either a transitive or intransitive sentence. The participants then answered a related question about general knowledge, and their response times were measured. The results show that, whereas the mean response time in the ordinary intransitive condition was significantly longer than that in the ordinary transitive condition, the mean response time in the agent-implying intransitive condition was not significantly different from that of the corresponding transitive condition, suggesting that agent-implying intransitive verbs are interpreted as agentive. The findings suggest that agent-implying intransitive verbs instantly evoke agentivity, whereas ordinary intransitive verbs do not. The theoretical implications of the findings are discussed.

Morphological Priming Effects in L2 English Verbs for Japanese-English Bilinguals.

For native (L1) English readers, masked presentations of past-tense verb primes (e.g., fell and looked) produce faster lexical decision latencies to their present-tense targets (e.g., FALL and LOOK) than orthographically related (e.g., fill and loose) or unrelated (e.g., master and bank) primes. This facilitation observed with morphologically related prime-target pairs (morphological priming) is generally taken as evidence for strong connections based on morphological relationships in the L1 lexicon. It is unclear, however, if similar, morphologically based, connections develop in non-native (L2) lexicons. Several earlier studies with L2 English readers have reported mixed results. The present experiments examine whether past-tense verb primes (both regular and irregular verbs) significantly facilitate target lexical decisions for Japanese-English bilinguals beyond any facilitation provided by prime-target orthographic similarity. Overall, past-tense verb primes facilitated lexical decisions to their present-tense targets relative to both orthographically related and unrelated primes. Replicating previous masked priming experiments with L2 readers, orthographically related primes also facilitated target recognition relative to unrelated primes, confirming that orthographic similarity facilitates L2 target recognition. The additional facilitation from past-tense verb primes beyond that provided by orthographic primes suggests that, in the L2 English lexicon, connections based on morphological relationships develop in a way that is similar to how they develop in the L1 English lexicon even though the connections and processing of lower level, lexical/orthographic information may differ. Further analyses involving L2 proficiency revealed that as L2 proficiency increased, orthographic facilitation was reduced, indicating that there is a decrease in the fuzziness in orthographic representations in the L2 lexicon with increased proficiency.