Primary Regulator Research Articles

The invertase gene PWIN1 confers chilling tolerance of rice at the booting stage via mediating pollen development.

Pollen fertility is a primary regulator of grain yield and is highly susceptible to cold and other environmental stress. We revealed the roles of rice cell wall invertase gene PWIN1 in pollen development and chilling tolerance. We uncovered its preferential expression in microspores and bicellular pollen and identified its knock-down and knock-out mutants. pwin1 mutants produced a higher proportion of abnormal pollen than wild-type plants. The contents of sucrose, glucose, and fructose were increased, while ATP content and primary metabolism activity were reduced in the mutant pollen. Furthermore, the loss of function of PWIN1 coincided with an increase in SnRK1 activity and a decrease in TOR activity. Under chilling conditions, pwin1 mutants displayed significantly reduced pollen viability and seed-setting rate, while overexpressing PWIN1 notably increased pollen viability and seed-setting rate as compared with the wild-type, indicating that PWIN1 is essential for rice pollen development and grain yield under cold stress. This study provides insights into the molecular mechanisms underlying rice pollen fertility during chilling stress, and a new module to improve chilling tolerance of rice at the booting stage by molecular design.

Application of Grid Forming Control in Energy Storage Systems for Primary Frequency Regulation Case Study: Galapagos Islands

Application of Grid Forming Control in Energy Storage Systems for Primary Frequency Regulation Case Study: Galapagos Islands

An optimizated additional damping control for suppressing ultra‐low frequency oscillation suppression based on SVC

AbstractThe ultra‐low frequency oscillation (ULFO) imposes an emerging stability challenge to the high proportion of hydropower grids. To suppress ULFO without reducing the primary frequency regulation of the hydro governor, a novel idea to exploit the voltage regulation effect of load is implemented. First, the additional damping controller is designed and configured in static var compensator (SVC), and the frequency analysis model considering the configuration of SVC as well as damping controller is established. Based on the model, the specific influence of SVC and controller on ULFO is analysed through eigenvalue calculation. In addition, the influence of various load models and SVC location on the damping level are further studied. Consequently, a parameter optimization design method for SVC additional damping control is proposed, it is modelled as the optimization problem of damping ratio in ULFO mode under multi‐operation conditions, which is solved by a particle swarm optimization algorithm. Finally, the effectiveness of the designed SVC additional damping controller is verified in the improved four‐machine two‐area power system and the actual power grid in China.

Joint Scheduling Method for Pumped Storage Units and Renewable Energy Sources

With the integration of renewable energy sources, how we can improve the stability of the new energy power system has become an urgent issue pursued by scholars. In this paper, a joint scheduling method for pumped storage units (PSUs) and renewable energy sources (RESs) considering frequency deviation and voltage stiffness constraints is proposed. First, the analytical expression of the frequency deviation constraint is derived based on the primary frequency regulation model. Then, the voltage stiffness constraint is constructed to describe the system’s capability of maintaining the voltage magnitude. Finally, to minimize the renewable energy abandonment caused by stability requirements, the joint scheduling model for PSUs and RESs is established. The application of the method ensures that the frequency deviation of the modified IEEE 39 bus system is less than 0.5 Hz, while the voltage stiffness remains above 0.9.

Wireless Power Transfer System with Constant Voltage/Constant Current Output Performance

Aiming at the problem of unstable output voltage and output current caused by load fluctuation, this paper starts from the perspective of system topology and control strategy for analysis. First, the output characteristics of LCL-S (inductor-capacitor-inductor and series), LCL-P (inductor-capacitor-inductor and parallel), LCL-LCL (inductor-capacitor-inductor and inductor-capacitor-inductor), and LCL-LCC (inductor-capacitor-inductor and inductor-capacitor-capacitor) compensation topologies are analyzed. Combining the advantages of LCL-S and LCL-LCL compensation topology output characteristics, an LCL-LCL/S compensation topology is constructed, and its topology is optimized. Then the influence of parasitic resistance on the output characteristics of LCL-LCL/S compensation topology is derived. A control strategy of primary side regulation is proposed to address the issue of unstable output voltage and current caused by parasitic resistance in the system under variable loads. This method can effectively improve the stability of the output voltage and output current of the wireless power transfer system in the LCL-LCL/S compensation topology under load fluctuations. Finally, a set of experimental prototypes is built to verify the correctness of the theoretical analysis.

A smooth method for primary frequency of wind turbine based on variable power point tracking and energy storage cooperative control

AbstractThe conventional deloading control has certain problems, such as low power generation efficiency and a small speed adjustment range. To improve the system's frequency quality and enhance the power grid's stability, this study comprehensively considers the effect of random source‐load power fluctuations on the system frequency. Moreover, this study proposes a smooth primary frequency control strategy for wind turbine based on the coordinated control of the variable power point tracking and supercapacitor energy storage. The impact of wind power fluctuations on the system frequency at different timescales for wind turbine is studied based on the historical data of wind power fluctuations in a strong wind meteorological cycle of a wind farm. The method determines the capacity of the energy storage device required for frequency smoothing at the optimal timescale. In combination with the required capacity of the wind turbine to participate in the system's primary frequency regulation, the supercapacitor energy storage device is optimally configured, and a set of supercapacitor energy storage device with the lowest cost under the highest charging/discharging efficiency is designed. Simulation and experimental results show that the primary frequency adjustment capability of the control strategy proposed in this study is significantly improved compared with the conventional primary frequency modulation control.

Day-ahead and hour-ahead optimal scheduling for battery storage of renewable energy power stations participating in primary frequency regulation

Day-ahead and hour-ahead optimal scheduling for battery storage of renewable energy power stations participating in primary frequency regulation

Frequency regulation feasible region assessment and optimization of wind farm based on data-driven model predictive control

With the integration of large-scale wind turbines (WTs) into grids via electronic interfaces, power system operators have necessitated frequency support from wind farms. Due to the large number of WTs and their complex dynamic characteristics, it is necessary to assess the primary frequency regulation (PFR) capability and construct feasible region of wind farms. In order to cope with the problems of incomplete parameters, analytical solving complexity and the coupling influence of power system regulation characteristics, this paper develops a data-driven state space mapping linear model predictive control (MPC) to assess the maximum PFR capability of wind farms and reasonably distribute coefficients to WTs. Besides, a coordinated iteration framework between dispatching center and wind farms is proposed to further optimize the wind farm regulation feasible region. The simulation results verify that the proposed method has the advantages of independence from physical parameters, fast analytical solution, and lower requirements of training samples on limited scenarios.

P-571 Mutations in FOXO3 mutation cause human premature ovarian insufficiency

Abstract Study question Do variants in front fork transcription factor 3 (FOXO3) account for premature ovarian insufficiency (POI) in humans? Is there is a potential treatment for POI caused by FOXO3 mutations? Summary answer Heterozygous variants in FOXO3 cause human female infertility owing to POI by inducing oocyte apoptosis. What is known already Front fork transcription factor 3 (FOXO3) is a member of the forkhead box family of transcription factors. Previous study had reported that FOXO3 encodes an evolutionarily conserved primary regulator and an effective inhibitor of primordial follicle activation. The null mutants of Foxo3 or its ortholog in multiple organisms displayed POI owing to deficiencies in maintaining the ovarian reserve. However, whether FOXO3 variants can underline POI phenotype in humans were unclear, and the causal relationship has not yet been established with functional evidence. Study design, size, duration 107 primary infertility families with POI were recruited for pathogenic variants screening. The proband with and her parents were verified for FOXO3 variants. Participants/materials, setting, methods All the patients were diagnosed with POI were confirmed by clinical analyses. Exome sequencing and subsequent bioinformatic analyses were performed to screen for candidate pathogenic variants. The pathogenicity of identified variants was assessed and studied in vivo in mice carrying the equivalent mutations. Main results and the role of chance Novel heterozygous variant in FOXO3 was identified in the patient with POI. Mice modeling the Foxo3 variant identified in patients recapitulated the ovarian reserve defects of patients, confirming the pathogenicity of the identified variants. The mutation caused constitutive activation of FOXO3 lead to POI by inducing oocyte apoptosis, which will facilitate the genetic diagnosis of POI in patients and provide a potential therapeutic target for female fertility. Limitations, reasons for caution A limitation of the current study is the small sample size. More primary infertility patients with POI should be screened to enrich the pathogenetic mutations of FOXO3. Wider implications of the findings FOXO3 was detected as a novel gene underlie human POI, thus providing new targets for the molecular diagnosis and treatment. Trial registration number the National Natural Science Foundation of China (NSFC) (Grant number:82302083)

Evaluation of primary frequency regulation in Dominican Republic mine using scooptrams and electric trucks as battery storage systems

The use of electric vehicles in the mining sector aligns with the growing demand for tools that reduce energy consumption in productive processes. This study investigates how electric mining vehicles with vehicle-to-grid (V2G) technology can be integrated into power grids to improve system stability and frequency regulation. In the context of mining operations and the provision of auxiliary services (such as primary frequency regulation) to an interconnected electrical system, a regulatory framework that enables V2G integration should be established. This issue is addressed through a case study involving DIgSILENT PowerFactory simulations. The goal is to evaluate the productive process of a mine and the contribution of V2G technology to the primary frequency regulation of the electrical system under different scooptram integration parameters and battery electric trucks. This study also explores the challenges of V2G implementation in the absence of a regulatory framework. Different configurations of electric scooptrams and shovel that can affect frequency regulation are assessed. The obtained optimal configuration involves two truck loaders and three shovel loaders; the capacity of the current electrical infrastructure can support this configuration without affecting the capacity of the mine's electrical system. The simulations verify that applying V2G technology to mining vehicles helps stabilise the frequency of electrical systems and reduces the probability of component failure.

Aerial signaling by plant-associated Streptomyces setonii WY228 regulates plant growth and enhances salt stress tolerance

Plant-associated streptomycetes play important roles in plant growth and development. However, knowledge of volatile-mediated crosstalk between Streptomyces spp. and plants remains limited. In this study, we investigated the impact of volatiles from nine endophytic Streptomyces strains on the growth and development of plants. One versatile strain, Streptomyces setonii WY228, was found to significantly promote the growth of Arabidopsis thaliana and tomato seedlings, confer salt tolerance, and induce early flowering and increased fruit yield following volatile treatment. Analysis of plant growth-promoting traits revealed that S. setonii WY228 could produce indole-3-acetic acid, siderophores, ACC deaminase, fix nitrogen, and solubilize inorganic phosphate. These capabilities were further confirmed through genome sequencing and analysis. Volatilome analysis indicated that the volatile organic compounds emitted from ISP-2 medium predominantly comprised sesquiterpenes and 2-ethyl-5-methylpyrazine. Further investigations showed that 2-ethyl-5-methylpyrazine and sesquiterpenoid volatiles were the primary regulators promoting growth, as confirmed by experiments using the terpene synthesis inhibitor phosphomycin, pure compounds, and comparisons of volatile components. Transcriptome analysis, combined with mutant and inhibitor studies, demonstrated that WY228 volatiles promoted root growth by activating Arabidopsis auxin signaling and polar transport, and enhanced root hair development through ethylene signaling activation. Additionally, it was confirmed that volatiles can stimulate plant abscisic acid signaling and activate the MYB75 transcription factor, thereby promoting anthocyanin synthesis and enhancing plant salt stress tolerance. Our findings suggest that aerial signaling-mediated plant growth promotion and abiotic stress tolerance represent potentially overlooked mechanisms of Streptomyces-plant interactions. This study also provides an exciting strategy for the regulation of plant growth and the improvement of horticultural crop yields within sustainable agricultural practices.

ErbB3 is required for hyperaminoacidemia-induced pancreatic α cell hyperplasia

Blood amino acid levels are maintained in a narrow physiological range. The pancreatic α cells have emerged as the primary aminoacidemia regulator through glucagon secretion to promote hepatic amino acid catabolism. Interruption of glucagon signaling disrupts the liver - α cells axis leading to hyperaminoacidemia, which triggers a compensatory rise in glucagon secretion and α cell hyperplasia. The mechanisms of hyperaminoacidemia-induced α cell hyperplasia remain incompletely understood. Using a mouse α cell line and in vivo studies in zebrafish and mice, we found that hyperaminoacidemia-induced α cell hyperplasia requires ErbB3 signaling. In addition to mTORC1, another ErbB3 downstream effector STAT3 also plays a role in α cell hyperplasia. Mechanistically, ErbB3 may partner with ErbB2 to stimulate cyclin D2 and suppress p27 via mTORC1 and STAT3. Our study identifies ErbB3 as a new regulator for hyperaminoacidemia-induced α cell proliferation and a critical component of the liver-α cells axis that regulates aminoacidemia.

Sexual Violence Crime Reform in Indonesia: Political and Legal Characteristics of Its Formation

Handling sexual violence and gender-based violence remains a significant issue in Indonesia. The state plays a crucial role in ensuring the enforcement of laws against sexual violence by enacting Law Number 12 of 2022 concerning Sexual Violence Crimes (UU TPKS). This study aims to identify the legal characteristics of UU TPKS. The study employs the literature review method. This research is descriptive-analytic, focusing on normative juridical legal research. The results show that the state is obliged to provide human rights protection, particularly in the context of eliminating sexual violence. UU TPKS is characterized as a responsive legal product. It fulfils the state's duty to provide comprehensive legal protection, encompassing prevention, resolution, and mitigation of both psychological and material losses suffered by victims of sexual violence due to the introduction of new formulations regarding sexual violence crimes that were not present in previous legislation. The criminal law reform in UU TPKS aims to change the existing legal substance to enhance the effectiveness of law enforcement, address crime, and tackle social and humanitarian issues. UU TPKS will become the primary and foremost special regulation in combating Sexual Violence Crimes, complementing the existing legislation that previously regulated the substance of sexual violence crimes.

DBF4, not DRF1, is the crucial regulator of CDC7 kinase at replication forks.

CDC7 kinase is crucial for DNA replication initiation and is involved in fork processing and replication stress response. Human CDC7 requires the binding of either DBF4 or DRF1 for its activity. However, it is unclear whether the two regulatory subunits target CDC7 to a specific set of substrates, thus having different biological functions, or if they act redundantly. Using genome editing technology, we generated isogenic cell lines deficient in either DBF4 or DRF1: these cells are viable but present signs of genomic instability, indicating that both can independently support CDC7 for bulk DNA replication. Nonetheless, DBF4-deficient cells show altered replication efficiency, partial deficiency in MCM helicase phosphorylation, and alterations in the replication timing of discrete genomic regions. Notably, we find that CDC7 function at replication forks is entirely dependent on DBF4 and not on DRF1. Thus, DBF4 is the primary regulator of CDC7 activity, mediating most of its functions in unperturbed DNA replication and upon replication interference.

Heat stress induces calcium dyshomeostasis to subsequent cognitive impairment through ERS-mediated apoptosis via SERCA/PERK/eIF2α pathway

Heat exposure is an environmental stressor that has been associated with cognitive impairment. However, the neural mechanisms that underlie this phenomenon have yet to be extensively investigated. The Morris water maze test was utilized to assess cognitive performance. RNA sequencing was employed to discover the primary regulators and pathological pathways involved in cognitive impairment caused by heat. Before heat exposure in vivo and in vitro, activation of the sarco/endoplasmic reticulum (SR/ER) calcium (Ca2+)-ATPase (SERCA) was achieved by CDN1163. Hematoxylin-Eosin, Nissl staining, calcium imaging, transmission electron microscopy, western blot, and immunofluorescence were utilized to visualize histological changes, intracellular calcium levels, endoplasmic reticulum stress (ERS) markers, apoptosis, and synaptic proteins alterations. Heat stress (HS) significantly induced cognitive decline and neuronal damage in mice. By the transcriptome sequencing between control (n = 5) and heat stress (n = 5) mice in hippocampal tissues, we identified a reduction in the expression of the atp2a gene encoding SERCA, accompanied by a corresponding decrease in its protein level. Consequently, this dysregulation resulted in an excessive accumulation of intracellular calcium ions. Furthermore, HS exposure also activated ERS and apoptosis, as evidenced by the upregulation of p-PERK, p-eIF2α, CHOP, and caspase-3. Consistently, a reduction in postsynaptic density protein 95 (PSD95) and synaptophysin (SYN) expressions indicated modifications in synaptic function. Notably, the impacts on neurons caused by HS were found to be mitigated by CDN1163 treatment both in vivo and in vitro. Additionally, SERCA-mediated ERS-induced apoptosis was attenuated by GSK2606414 treatment via inhibiting PERK-eIF2α-CHOP axis that not only curtailed the level of caspase-3 but also elevated the levels of PSD95 and SYN. These findings highlight the significant impact of heat stress on cognitive impairment, and further elucidate the underlying mechanism involving SERCA/PERK/eIF2α pathway.

Advanced Primary Frequency Regulation Optimization in Wind Storage Systems with DC Integration Using Double Deep Q-Networks

With the gradual increase in wind power installation capacity, the proportion of traditional synchronous generators driven by fossil fuel is gradually declining. Due to the fact that wind turbines are connected to the grid through power electronic converters, which decouple rotor speeds from the system frequency and reduce system inertia levels, inadequate inertia levels can pose a threat to frequency stability when disturbances occur. To address this issue, this paper proposes a frequency regulation optimization strategy for the direct current (DC) transmission of a wind storage system. This strategy incorporates virtual inertia control and virtual droop control to adjust wind power output based on frequency deviation and rate of change. Fuzzy logic control is employed for energy storage, adaptively adjusting active power based on frequency deviation and the rate of change. Additionally, under the context of multi-DC transmission in renewable energy systems, an optimization strategy for proportion and integration (PI) parameters of the frequency limit controller (FLC) is proposed. Considering frequency deviation and DC regulation power simultaneously, the double deep Q-network (DDQN) algorithm is adopted in the simulation model to attain the optimal parameters of FLC. Simulation results conducted using MATLAB/Simulink 2022a indicate that this strategy increases the lowest frequency by 0.28 Hz and decreases the response time by 1.04 s compared with the non-optimized strategy.

Can the Presence of Big 4 Auditors in IPO Prospectus Reduce Failure Risk?

This paper addresses a void in the research on auditing and initial public offering (IPO) failure by investigating the impact of the Big 4 auditing firms on the likelihood of an IPO failure. This research is the first comprehensive analysis of more than 33,000 global IPOs that either failed or were successful between 1995 and 2019 across a wide range of nations with vastly different regulatory, cultural, and economic settings. A cross-sectional probit regression model is utilized to investigate the influence of hiring the Big 4 auditing firms on IPO failure, building upon prior studies on IPO failure. We found strong evidence that IPO failure rates were diminished by up to 67% when one of the Big 4 auditing firms was involved in auditing the IPO prospectus. For IPO founders, hiring Big 4 auditors before an IPO is a quality signaling strategy that minimizes the risk of a failed IPO by reducing information asymmetry among IPO participants. Our findings provide useful policy implications. Hiring one of the Big 4 auditing firms before an IPO is a reassuring signaling strategy for founders, since it decreases information asymmetry among IPO investors and so lowers the risk of the IPO failing. Primary market investors now have access to credible evidence indicating that backing IPOs from companies that use the Big 4 auditing firms increases the likelihood of such IPOs being listed on stock exchanges and yields positive returns. This is the first time, as far as the academicians are aware, that conclusive evidence has been found of a strong inverse association between the presence of Big 4 audits and failure risk for IPO firms. Our research could be helpful to primary market regulators since it shows how crucial it is to encourage Big 4 audits in IPO companies. The quality work of the Big 4 auditors does lower the risk of failure in the IPO market, which might help owners of small private equities to list their firms on the IPO market, boosting economic growth.

Kepemilikan Rumah Hunian Oleh Orang Asing Diatas Hak Milik

The need for residential housing for foreigners who reside is a basic right that should be considered. Foreigners who have competencies in the field of tourism, managers, and other expertise for the Indonesian state are very realistic because the knowledge of foreigners' work skills for progress is mostly owned by foreigners. Indonesia as a country that will lead to progress is in dire need of knowledge and skills in various fields of technology, management and increasing resources both human and natural resources. The research method is carried out with the type of normative legal research method, with the source of primary legal material Government Regulation of the Republic of Indonesia Number 103 of 2015 concerning Ownership of Residential Houses or Occupancy by Foreigners Residing in Indonesia, and other laws and regulations related to the issues discussed. While secondary legal materials are library books, journal articles on line journal system, scientific papers, and reviews in the mass media and others. The results of the study indicate that there is conformity between the Republic of Indonesia Government Regulation Number 103 and the Republic of Indonesia Government Regulation in Article 4 letter a and number 1 of Number 10 of 1996 concerning Cultivation Rights, Building Rights and Land Rights Article 39 letter e and land objects Article 40 letter c, as well as the Republic of Indonesia Government Regulation Number 18 of 2021 concerning Land Management Rights, Land Rights, Flat Housing Units and Land Registration Article 49 paragraph (1).

A wind farm planning method considering frequency security constraint based on wind farm participation in primary frequency regulation

Abstract Renewable energy sources such as wind power, connected to the grid on a large scale, reduce the rotational inertia and frequency regulation of the power system. Many studies have included frequency security constraints (FSC) in power system scheduling to ensure system frequency stability, but few studies have included FSC in grid planning. Therefore, this paper establishes a planning model for wind farms that considers the FSC based on the wind turbines’ participation in the primary frequency regulation (PFR) by obtaining the maximum deviation value constraint of the system frequency through the system frequency response (SFR) model. Tests were conducted on the IEE30-node system, and the results show that the proposed model enhances the system frequency response capability and ensures system security.

Lysophosphatidic acid promotes ESCC progression by increasing the level of CCL2 secreted by esophageal epithelial cells.

Lysophosphatidic acid (LPA) is a small bioactive lipid which acts as a potent regulator in various tumor progressions through six G-protein-coupled receptors (LPA1-LPA6). Our previous study demonstrated that the LPA-producing enzyme, autotaxin (ATX), was upregulated in esophageal squamous cell carcinoma (ESCC) and ATX high expression levels indicated a poor prognosis. Esophageal squamous cell carcinoma is a type of malignant tumor which originates from epithelial cells. Its progression can be affected by the interaction between cancer cells and normal cells. However, the impact of LPA on the interaction between esophageal epithelial cells and cancer cells in the development of ESCC remains uncertain. MTS and Edu assays were performed to determine ESCC cell proliferation in culture medium (CM) derived from LPA-stimulated esophageal epithelial cells (Het-1a). A wound healing assay, transwell migration and an invasion assay were performed to assess the metastatic ability of ESCC cells. Cytokine array analysis was conducted to detect the differentially secreted cytokines in CM. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were utilized to uncover the pathways and cytokines that are influenced by LPA in ESCC. Immunohistochemical staining was employed to measure the expression of ATX and CCL2 in early-stage ESCC. Quantitative real-time PCR, western blot, enzyme-linked immunosorbent assay and an antibody neutralization assay were employed to measure the mechanism of LPA-mediated communication between epithelial cells and cancer cells. Functional experiments showed that exposing ESCC cancer cells to CM from LPA-treated Het-1a results in promoting proliferation, migration, invasion and epithelial-mesenchymal transition processes. Using cytokine array analysis, we discovered that LPA triggers the release of multiple cytokines from epithelial cells. After screening of the TCGA and GEO databases, CCL2 was identified and found to be correlated with ATX expression in ESCC. Furthermore, CCL2 levels in both mRNA expression and secretion were observed to be upregulated in epithelial cells upon stimulation with LPA. Blocking CCL2 effectively reduced the pro-migration influence of CM derived from LPA-treated Het-1a. Mechanism studies have demonstrated that LPA activated the NF-κB signaling pathway through LPA1/3, ultimately causing an increase in CCL2 expression and secretion in Het-1a. Our findings, taken together, demonstrate that CM from LPA-treated esophageal epithelial cells plays a significant role in promoting the progression of ESCC, with CCL2 acting as the primary regulator.