Primary Protein Source Research Articles

Prevalence of Abomasum Nematode infection in Sheep from North of Iran

Abstract
 In many countries, small ruminants provide the primary source of human protein and play an important role in livestock production. Parasitic infections, especially gastrointestinal parasites, cause economic losses such as reduced fertility and milk production, damage to the leather industry, weight loss, treatment costs, and losses in severe infections. Thus, identifying and classifying these parasites, including the above cases, is essential for scientific research in any country. This study aimed to Prevalence of Abomasum Nematode infection in Sheep from North of Iran. In the present cross-sectional descriptive study, we have studied the prevalence of infection with abomasum nematodes in sheep in northern Iran from April 2020 to September 2021 in 2400 abomasum sheep slaughtered in industrial slaughterhouses in Gilan and Mazandaran provinces (1200 sheep from each province). With three age groups of below two years, two to four years, and above four years and gender segregation of hosts, after necropsy and preparation of sheep abomasal mucosa chip and isolation and segregation of samples, we studied the morphologic and morphometric characteristics of the samples. The actions were performed according to the taxonomic keys and using SPSS software V.16 and Paired Sample T-Test statistical test to determine the level of significance of the data (p <0.05) to classify and compare the obtained data and examine the ratio. The sex of the host was determined by the age of the animal and the prevalence of the parasite at the mentioned ages. The present study results and comparison of the prevalence of infection in Gilan and Mazandaran provinces showed that Marshallagia marshalli had the highest prevalence of 94.6% and 91.9% in Mazandaran and Gilan province, respectively. The prevalence of Ostertagia circumcincta was 87.2% and 77.2% in Gilan and Mazandaran provinces. The prevalence of Ostertagia occidentalis was 86.9% in Gilan province and 78% in Mazandaran province, while the prevalence of Parabronema skrjabini was 86.6% in Gilan province and 61.2% in Mazandaran province. The prevalence of Haemonchus contortus was 66.8% and 60.5% in Gilan and Mazandaran, the lowest prevalence in these two provinces. The present study results showed the importance of combating the spread of parasitic infections with strategic treatment methods and biological control of these parasites. Also, various reports of drug resistance in abomasal nematodes should be emphasized more and more by competent bodies.
 Keywords: Abomasum, Nematodes, North of Iran, Parasitic infections, Sheep.

Fish Fillet: White Versus Red, Structure and Nutritional Composition

Fish is one of the primary sources of protein and other nutrients globally. There are thousands of food fish species with different chemical compositions and nutritional characteristics of fillets. Therefore, this EDIS publication has provided information on fish muscle structure and differences between red muscles and white muscles in fish. In addition, discussions on fish muscle composition and the effect of the heat on the muscle are provided.

Watershed Development-Present Status and Fisheries Perspectives in Indian Context

India encompasses a rich aquatic biodiversity, hosting vast arrays of freshwater, brackish water, and marine ecosystems. These unique landscapes harbor abundant fisheries resources, constituting primary protein sources and income channels for millions. Fisheries are an essential source of food security, livelihoods, employment generation, recreation, trade, and foreign exchange earnings worldwide. However, over exploitation, pollution, habitat destruction, climate change, invasive species, and other anthropogenic activities threaten fisheries resources’ sustainability. Therefore, integrated approaches like watershed management can help restore and maintain aquatic ecosystem health, ensuring sustainable fish production while enhancing social welfare. This article explores the significance of watershed development in conserving fisheries resources and promoting their long-term viability.Watershed development plays a pivotal role in nurturing sustainable aquatic ecosystems and enhancing fisheries production in India. Amid intensified exploitative pressures coupled with changing climatic scenarios, watershed development emerges as an integral intervention toward sustainable fisheries conservation and management. Despite considerable progress made, certain constraints persist, necessitating targeted strategies for optimal outcomes. This article evaluates the current scenario, future possibilities, and viewpoints concerning watershed development vis-a-vis fisheries in India.

Tapping Chlorella vulgaris potential for enhanced growth, immunity, digestion, microbiota, and immunometabolism in Litopenaeus vannamei feeding across varied salinities

This study explored the use of Chlorella vulgaris (CHL) in Litopenaeus vannamei under varying salinity conditions. Juvenile L. vannamei (initial weight 0.38 ± 0.01 g) were fed experimental diets for 8 weeks at different salinity levels: low (15‰), moderate (30‰), and high (45‰). The control diet contained 589 g/kg fishmeal (FM) as the sole protein source, while experimental diets included 150 g/kg FM and 492 g/kg CHL as protein sources. Results revealed that shrimp fed with CHL showed a robust survival rate even at higher salinities. Following a Vibrio parahaemolyticus infection challenge, shrimp fed with CHL exhibited higher survival rates than those fed with FM at equivalent salinities. Enzyme activity and gene expression analyses indicated that CHL enhanced immune response and digestion in L. vannamei compared to the FM, even under similar salinity conditions. Additionally, shrimp fed with CHL outperformed those fed with FM under varying salinity stress. Microbiota analysis revealed increased diversity and beneficial bacteria in the intestines of shrimp fed with CHL at the same salinity levels. Transcriptomic sequencing identified genes with differential expression (DEGs) related to immune and metabolic pathways. Furthermore, a combined analysis of microbiota and DEGs revealed that CHL also had a beneficial impact on the composition of the intestinal microbiota and controlled the expression of genes related to immune and metabolic pathways. In summary, CHL as a primary protein source improved survival rate, disease resistance, immune response, and digestion in L. vannamei, positively impacting intestinal microbiota and gene expression, and enhancing adaptability to salinity changes.

Organic Ingredients as Alternative Protein Sources in the Diet of Juvenile Organic Seabass (Dicentrarchus labrax).

The use of organic ingredients as a source of protein in aquaculture diets has gained significant attention due to the growing demand for organic seafood products. This study aimed to evaluate the potential for the use of organic ingredients as protein sources in the diet of juvenile organic seabass (Dicentrarchus labrax). A total of 486 juvenile seabass with an average weight of 90 g were fed six diets containing varied organic proteins. The control group (CON) was fed a diet with conventional fishmeal from sustainable fisheries as the primary protein source. The other five groups were fed diets with different compositions: organic Iberian pig meal byproduct (IB diet), a combination of organic Iberian pig meal byproduct and insect meal (IB-IN diet), a mix of organic Iberian pig meal byproduct and organic rainbow trout meal byproduct (IB-TR diet), a blend of organic rainbow trout meal byproduct and insect meal (TR-IN), and a mixed diet containing all of these protein sources (MIX diet). Over a 125-day feeding trial, growth performance, feed utilisation, feed digestibility, and histological parameters were assessed. The results showed that the fish fed the control diet had the highest final weight and specific growth rate, followed by the fish fed the TR-IN and IB-TR diets. The IB-TR diet had the highest apparent digestibility coefficients (ADCs) for protein, while the TR-IN diet had the lowest. Histological analysis revealed that fish fed the control diet had the largest nucleus diameter and hepatocyte diameter. Use of IN seems to penalise performance in several ways. Fish fed diets containing insect meal grew less, and those diets had lower digestibility. Fish fed the TR and IB diets grew at rates near that of the control, and the feed had acceptable digestibility.

The potential of young vegetative quinoa (Chenopodium quinoa) as a new sustainable protein-rich winter leafy crop under Mediterranean climate

The demand for protein products has significantly risen in the last few years. In western countries, animals are the primary source of protein; however, plants could take a share of this market due to lower production costs, among other advantages such as a lower environmental footprint. Quinoa (Chenopodium quinoa Willd.) is a well-known but under-utilized protein-rich crop, commonly cultivated for grain production. These plants were recently evaluated for their use as a non-traditional, green leafy crop. Here we assessed the potential of young vegetative quinoa as a new sustainable winter leafy crop in Israel—serving as a model for Mediterranean semi-arid regions, by evaluating yield, protein content and quality. Five quinoa accessions were sown on three winter sowing dates over two consecutive years. Plants were harvested when they reached 10% dry matter (DM). DM yield ranged between 574 and 1,982 kg ha-1 and was generally higher in the second year. Protein content ranged from 14.4–34% and was generally higher in the first year. Protein yield ranged from 111–471 kg ha-1 and was greatest on the December sowing date. DM and protein yields were positively correlated with plant density. Protein content was negatively correlated with plant density and DM yield. Our findings show that 200 g DM of young vegetative quinoa can meet the protein and most essential amino acid requirements for a 70 kg human adult. Prospects for cultivating young vegetative quinoa in Mediterranean countries as a new sustainable, protein-rich winter leafy crop are therefore high, as supported by its high protein yields and quality, and its requirement for only scant irrigation. Further studies should examine economic and other agrotechnical parameters toward the geographical distribution and expansion of young vegetative quinoa cultivation.

Investigating the expression of fertility-regulating LncRNAs in multiparous and uniparous Shal ewe's ovaries.

Sheep is the primary source of animal protein in Iran. Birth type is one of the significant features that determine total meat output. Little is known about how long non-coding RNAs (LncRNAs) affect litter size. The purpose of this research is to investigate the DE-LncRNAs in ovarian tissue between multiparous and uniparous Shal ewes. Through bioinformatics analyses, LncRNAs with variable expression levels between ewes were discovered. Target genes were annotated using the DAVID database, and STRING and Cytoscape software were used to evaluate their interactions. The expression levels of 148 LncRNAs were different in the multiparous and uniparous ewe groups (false discovery rate (FDR)<0.05). Eight biological process terms, nine cellular component terms, 10 molecular function terms, and 38 KEGG pathways were significant (FDR<0.05) in the GO analysis. One of the most significant processes impacting fertility is mitogen-activated protein kinase (MAPK) signaling pathway, followed by oocyte meiosis, gonadotropin-releasing hormone signaling pathway, progesterone-mediated oocyte maturation, oxytocin signaling pathway, and cAMP signaling pathway. ENSOARG00000025710, ENSOARG00000025667, ENSOARG00000026034, and ENSOARG00000026632 are LncRNAs that may affect litter size and fertility. The most crucial hub genes include MAPK1, BRD2, GAK, RAP1B, FGF2, RAP1B, and RAP1B. We hope that this study will encourage researchers to further investigate the effect of LncRNAs on fertility.

Adapting the marine stewardship council's risk-based framework to assess the impact of towed bottom fishing gear on blue carbon habitats.

Wild capture fisheries are of economic and social importance, providing a primary source of protein to people globally. There is a broad research base on the environmental impacts of fishing gears and processing methods yet, the impact on the global CO2 budget is less well studied. Evaluating the risk that wild capture fisheries pose to ecosystem health is vital to sustainably managing fishing practices to meet increasing global nutritional needs and reverse declines in marine biodiversity. At the same time meeting net-zero ambitions by reducing direct and indirect GHG emissions is vital. Ecological risk assessments, trait-based assessments, and vulnerability assessments have long supported fisheries management systems globally but do not yet provide any representation regarding the impacts that fishing gears have on the ability of the habitat to capture and store carbon. Considering the importance of accessibility and transparency in approaches necessary for fisheries sustainability certifications, this paper describes a method to integrate habitat carbon capacity attributes into the Marine Stewardship Council (MSC) Consequence and Spatial Analysis (CSA) framework. Applying the CSA carbon extension developed herein produces different CSA risk scores compared to the MSC CSA that does not account for carbon. This has potential consequences for certification schemes as carbon becomes more important in the fisheries sustainability conversation. The CSA carbon extension tool developed here is an important first step in incorporating carbon indicators into evaluations of fisheries that consider fishery carbon impacts.

Higher Muscle Protein Synthesis Rates Following Ingestion of an Omnivorous Meal Compared with an Isocaloric and Isonitrogenous Vegan Meal in Healthy, Older Adults

BackgroundPlant-derived proteins are considered to have fewer anabolic properties when compared with animal-derived proteins. The anabolic properties of isolated proteins do not necessarily reflect the anabolic response to the ingestion of whole foods. The presence or absence of the various components that constitute the whole-food matrix can strongly impact protein digestion and amino acid absorption and, as such, modulate postprandial muscle protein synthesis rates. So far, no study has compared the anabolic response following ingestion of an omnivorous compared with a vegan meal. ObjectivesThis study aimed to compare postprandial muscle protein synthesis rates following ingestion of a whole-food omnivorous meal providing 100 g lean ground beef with an isonitrogenous, isocaloric whole-food vegan meal in healthy, older adults. MethodsIn a randomized, counter-balanced, cross-over design, 16 older (65–85 y) adults (8 males, 8 females) underwent 2 test days. On one day, participants consumed a whole-food omnivorous meal containing beef as the primary source of protein (0.45 g protein/kg body mass; MEAT). On the other day, participants consumed an isonitrogenous and isocaloric whole-food vegan meal (PLANT). Primed continuous L-[ring-13C6]-phenylalanine infusions were applied with blood and muscle biopsies being collected frequently for 6 h to assess postprandial plasma amino acid profiles and muscle protein synthesis rates. Data are presented as means ± standard deviations and were analyzed by 2 way-repeated measures analysis of variance and paired-samples t tests. ResultsMEAT increased plasma essential amino acid concentrations more than PLANT over the 6-h postprandial period (incremental area under curve 87 ± 37 compared with 38 ± 54 mmol·6 h/L, respectively; P-interaction < 0.01). Ingestion of MEAT resulted in ∼47% higher postprandial muscle protein synthesis rates when compared with the ingestion of PLANT (0.052 ± 0.023 and 0.035 ± 0.021 %/h, respectively; paired-samples t test: P = 0.037). ConclusionsIngestion of a whole-food omnivorous meal containing beef results in greater postprandial muscle protein synthesis rates when compared with the ingestion of an isonitrogenous whole-food vegan meal in healthy, older adults.This study was registered at clinicaltrials.gov as NCT05151887.

PSVI-6 Effects of Partial or Total Replacement of Soybean Meal with Urea on Feed Efficiency in Nellore × Red Angus Growing Bulls

Abstract Soybean meal (SBM) is the primary protein source used worldwide for livestock. but is a high-cost protein source than other alternative protein sources such as urea. This study aimed to evaluate the effects of partial or total replacement of SBM with urea on dry matter intake (DMI), average daily gain (ADG) and feed efficiency of finishing Nellore × Red Angus crossbreed bulls. Nellore × Red Angus crossbred bulls (n = 30; average initial BW = 383.5 ± 8 kg; age = 9 ± 1 mo) were utilized in a completely randomized design for 93 days (9 d adaptation period + 84 d finishing period). Bulls were randomly assigned to receive 1 of 3 dietary treatments containing different replacement levels of soybean meal with urea (diet DM basis): 0% replacement (R-0%); 50% replacement (R-50%); 100% replacement (R-100%). Diets were formulated according to the BR-CORTE system to achieve an ADG of 1.3 kg and to contain 130 g CP/kg DM. The diets consisted of 20% corn silage and 80% concentrate that was formulated with ground corn, soybean meal and/or urea, ammonium sulfate, mineral mix, and virginiamycin. All animals were fed for ad libitum intake using an electronic feeder (INTERGADO, Intergado Ltd., Contagem, Minas Gerais, Brazil) and individual daily feed intake was recorded. The bulls were weighed at the beginning and end and every 28 d throughout the finishing period. Data were analyzed using the MIXED procedure of SAS (SAS Inst. Inc., Cary, NC) assuming the significance level of 0.05. There was no influence on final BW (P = 0.39), DMI (P = 0.22), ADG (P = 0.47), or feed efficiency (P = 0.98; Table 1). These results suggest that SBM can be replaced with urea and may be used as a strategy to reduce diet costs for Nellore × Red Angus crossbred bulls without affecting DMI, ADG, and feed efficiency.

Transcriptome analysis of the allometric growth of golden pompano (Trachinotus ovatus) following soybean meal feed

Golden pompano (Trachinotus ovatus) is a marine fish of great commercial value. It was selected for a study on allometric growth using fermented soybean meal (SBM) as the primary protein source during an 8-week culture period. By comparing the gene expression levels of different individuals in the fermented soybean meal group, we discovered that 1026 genes exhibited significant changes in slow and fast-growth individuals. Among these genes, 358 showed high expression levels, while 668 showed low ones. Subsequently, we conducted go function annotation and KEGG pathway enrichment analysis on all the significantly differentially expressed genes. This analysis revealed that many of these genes were associated with oxidative phosphorylation, steroid biosynthesis, glyceride metabolism, alanine, aspartic acid, and glutamate metabolism. Identifying these differentially expressed genes is a foundation for unraveling the molecular mechanisms behind growth and development. Additionally, it provides valuable gene data for future studies on the cloning and expression of growth-related genes and offers insights for subsequent biological research.

Exploiting Indian landraces to develop biofortified grain sorghum with high protein and minerals.

Sorghum (Sorghum bicolor L. Moench) is the staple cereal and is the primary source of protein for millions of people in Asia and sub-Saharan Africa. Sorghum grain value has been increasing in tropical countries including India owing to its gluten-free nature, anti-oxidant properties and low glycemic index. However, the nutrient composition of modern cultivars is declining thus necessitating genetic biofortification of sorghum to combat malnutrition and improve nutritional balance in the human diet. Keeping this in view, efforts were made to utilize valuable alleles, associated with nutrient composition, that might have been left behind in the varietal development in sorghum. The study aimed to determine the genetic improvement for nine nutritional and quality parameters (crude protein, in vitro protein digestibility (IVPD), total iron (Fe), total zinc (Zn), bioavailable Fe (%), bioavailable Zn (%), total phenolics, tannins and antioxidant activity) in the grains of 19 sorghum genotypes (high yield, drought and grain mold tolerant) developed from 11 superior India's landraces. After selection and advancement made from 2017 to 2022 through single seed descent method, the improvement in the nine nutritional and quality parameters was assessed. Significant variation was observed for all the nine parameters among the landraces and the genotypes. Sorghum genotypes PYPS 2 and PYPS 13 recorded the highest crude protein (13.21 and 12.80% respectively) and IVPD (18.68 and 19.56% respectively). Majority of the sorghum genotypes recorded high Fe (14.21-28.41 mg/100 g) and Zn (4.81-8.16 mg/100 g). High phenolics and antioxidant activity were recorded in sorghum genotypes PYPS 18 (85.65 mg/g gallic acid equivalents) and PYPS 19 (89.78%) respectively. Selections through SSD method revealed highest improvement in genotype PYPS 10 for crude protein (32.25%), total phenolics (18.48%) and antioxidant activity (15.43%). High improvements in genotypes PYPS 12 (23.50%), PYPS 3 (26.79%), PYPS 15 (21.18%) were recorded for total Fe, available Fe and high tannins, respectively. The study demonstrated that landraces could be effectively utilized as a potential, low-cost and eco-friendly approach in sorghum genetic biofortification to improved sorghum productivity and nutritional supply in semi-arid tropics.

Genome-wide association study and high-quality gene mining related to soybean protein and fat

BackgroundSoybean is one of the most important oil crops in the world, and its protein and fat are the primary sources of edible oil and vegetable protein. The effective components in soybean protein and fat have positive effects on improving human immunity, anti-tumor, and regulating blood lipids and metabolism. Therefore, increasing the contents of protein and fat in soybeans is essential for improving the quality of soybeans.ResultsThis study selected 292 soybean lines from different regions as experimental materials, based on SLAF-seq sequencing technology, and performed genome-wide association study (GWAS) on the phenotype data from 2019–2021 Planted at the experimental base of Jilin Agricultural University, such as the contents of protein and fat of soybeans. Through the GLM model and MLM model, four SNP sites (Gm09_39012959, Gm12_35492373, Gm16_9297124, and Gm20_24678362) that were significantly related to soybean fat content were associated for three consecutive years, and two SNP sites (Gm09_39012959 and Gm20_24678362) that were significantly related to soybean protein content were associated. By the annotation and enrichment of genes within the 100 Kb region of SNP loci flanking, two genes (Glyma.09G158100 and Glyma.09G158200) related to soybean protein synthesis and one gene (Glyma.12G180200) related to lipid metabolism were selected. By the preliminary verification of expression levels of genes with qPCR, it is found that during the periods of R6 and R7 of the accumulation of soybean protein and fat, Glyma.09G158100 and Glyma.09G158200 are positive regulatory genes that promote protein synthesis and accumulation, while Glyma.12G180200 is the negative regulatory gene that inhibits fat accumulation.ConclusionsThese results lay the basis for further verifying the gene function and studying the molecular mechanisms regulating the accumulation of protein and fat in soybean seeds.

A Comparison of Fish Ponds in the Lailan, Daqoq, Yayji, and Sarkaran Areas in Terms of Temperature and Ph

Food security and increased fish production are critical issues for many countries, including Iraq, where many rely on fish as a primary source of protein and nutrition. To achieve this goal efficiently and sustainably, it is essential to understand and monitor the environmental factors that affect the success of fish farming. This study analyzed water temperature and pH levels in several fish ponds in the Lailan, Daqoq, Yayji, and Sarkaran regions of Iraq. Monthly and spatial variations in these environmental factors were studied over several months in the year, allowing an understanding of their impact on the aquatic environment and fish health. The results showed monthly and spatial variations in water temperature and pH levels among the studied regions. These results were compared with international standards for fish water quality, and it was concluded that most of the ponds met these standards, suggesting the potential for improving fish farming practices and increasing production. Furthermore, the study highlighted the importance of achieving a balance in water temperature and pH levels to maintain fish health and enhance the success of farming operations. These findings can contribute to agricultural sustainability, provide food resources for local communities, and achieve food security in the region. Understanding the relationship between water temperature and pH levels and fish health and productivity is a crucial step towards enhancing the fish farming sector in Iraq and meeting the increasing food needs of the population [1].

A Comparison of Fish Ponds in the Lailan, Daqoq, Yayji, and Sarkaran Areas in Terms of Temperature and Ph

Food security and increased fish production are critical issues for many countries, including Iraq, where many rely on fish as a primary source of protein and nutrition. To achieve this goal efficiently and sustainably, it is essential to understand and monitor the environmental factors that affect the success of fish farming. This study analyzed water temperature and pH levels in several fish ponds in the Lailan, Daqoq, Yayji, and Sarkaran regions of Iraq. Monthly and spatial variations in these environmental factors were studied over several months in the year, allowing an understanding of their impact on the aquatic environment and fish health. The results showed monthly and spatial variations in water temperature and pH levels among the studied regions. These results were compared with international standards for fish water quality, and it was concluded that most of the ponds met these standards, suggesting the potential for improving fish farming practices and increasing production. Furthermore, the study highlighted the importance of achieving a balance in water temperature and pH levels to maintain fish health and enhance the success of farming operations. These findings can contribute to agricultural sustainability, provide food resources for local communities, and achieve food security in the region. Understanding the relationship between water temperature and pH levels and fish health and productivity is a crucial step towards enhancing the fish farming sector in Iraq and meeting the increasing food needs of the population [1].

High efficient broad-spectrum Bradyrhizobium elkanii Y63-1

Soybean (Glycine max), the primary source of high-quality plant protein, plays a crucial role as a grain and oil crop in China. Harnessing the full potential of symbiotic nitrogen fixation in soybean production holds immense significance for agriculture and ecology alike. Zhongdou 63, a newly developed early-maturing summer soybean cultivar in 2021, exhibits remarkable traits such as high yield, superior quality, multi-resistance, and wide adaptability. In this study, eight distinct rhizobia strains from diverse regions were meticulously screened to identify highly effective strains specifically suited for Zhongdou 63. The aboveground biomass, plant height, chlorophyll content, root length, nodule number, and nodule dry weight of Zhongdou 63 were measured and the data were subjected to statistical analysis. The results demonstrated that Y63-1 is a predominant strain of Zhongdou 63. Subsequently, we conducted further investigations on the broad-spectrum nodulation characteristics of Y63-1. Ten representative soybean cultivars were individually inoculated with Y63-1 and subsequently analyzed for nodule numbers and nodule dry weight in their symbiotic systems with rhizobia. The findings revealed that Y63-1 effectively formed nodules with all ten soybean varieties tested. In summary, our current study identified highly efficient broad-spectrum Bradyrhizobium elkanii strain Y63-1 as the predominant strain in Zhongdou 63 and provided a theoretical foundation for enhancing yield potential not only in Zhongdou 63 but also in other varieties through inoculation with highly efficient rhizobia in production.

Protein Hydrolysate from Underutilized Legumes: Unleashing the Potential for Future Functional Foods.

Proteins play a vital role in human development, growth, and overall health. Traditionally, animal-derived proteins were considered the primary source of dietary protein. However, in recent years, there has been a remarkable shift in dietary consumption patterns, with a growing preference for plant-based protein sources. This shift has resulted in a significant increase in the production of plant proteins in the food sector. Consequently, there has been a surge in research exploring various plant sources, particularly wild, and underutilized legumes such as Canavalia, Psophocarpus, Cajanus, Lablab, Phaseolus, and Vigna, due to their exceptional nutraceutical value. This review presents the latest insights into innovative approaches used to extract proteins from underutilized legumes. Furthermore, it highlights the purification of protein hydrolysate using Fast Protein Liquid Chromatography. This review also covers the characterization of purified peptides, including their molecular weight, amino acid composition, and the creation of three-dimensional models based on amino acid sequences. The potential of underutilized legume protein hydrolysates as functional ingredients in the food industry is a key focus of this review. By incorporating these protein sources into food production, we can foster sustainable and healthy practices while minimizing environmental impact. The investigation of underutilized legumes offers exciting possibilities for future research and development in this area, further enhancing the utilization of plant-based protein sources.

Pork Consumption Patterns among Rural Residents in China: A Regional and Cultural Perspective (2000–2020)

Pork is a principal component of the food supply for residents in China, acting as a primary source of animal protein. Analyzing the factors affecting pork consumption among rural Chinese residents is critical for understanding trends in the pig market and the direction of price control. China encompasses an immense geographical area (ranked third in the world) and is home to a globally acknowledged cultural diversity. This results in China having a diverse range of regional and cultural characteristics. These factors may profoundly influence the meat consumption habits of rural residents in China in a real-world context. In econometric decision making, traditional fixed effects models are incapable of estimating regional and cultural factors as these come under individual effects. In this study, leveraging a distinct fixed-effect filter model recently developed, we estimate these regional and cultural variables to verify whether they are statistically significant. This approach adds a fresh perspective to traditional consumption theory. This study uses panel data from 31 provinces, municipalities, and autonomous regions registered in national data as the research object. The detailed findings include: (1) Rural residents in coastal provinces show a stronger inclination towards pork consumption compared to those in inland areas. (2) The ethnic feature of provinces also presents a significant impact, with provinces reflecting Mongolian, Zhuang, Tujia, and Yi ethnic characteristics pointing to higher pork consumption. This contrasts with provinces characterized by Yao, Li, and Muslim ethnicities, which tend towards the opposite. Based on these empirical findings, this paper provides policy suggestions for optimizing the layout of the pig industry, which will offer multi-dimensional regulatory directions for optimizing the pig industry layout in China.

Pre-illness dietary risk factors in dogs with chronic enteropathy.

Dietary factors have been extensively studied as potential triggers of inflammatory bowel disease in humans. Scant literature exists regarding diet as a pre-illness risk factor in dogs with chronic enteropathy (CE). To evaluate possible pre-illness dietary risk factors in dogs with CE. Ninety-five client-owned dogs; 48 with CE (25 presumptive and 23 confirmed) and 47 without a history of signs of gastrointestinal disease. Retrospective case-control questionnaire-based study at a veterinary referral teaching hospital in the United Kingdom. Diet history was obtained relating to the onset of initial presenting signs for all dogs. The main diet consumed underwent ingredient analysis and caloric distribution calculation using a guaranteed analysis convertor software. Length of time the main diet was fed and adherence to the World Small Animal Veterinary Association Global Nutrition Committee guidelines was also recorded. The frequency of the main diet containing no carbohydrate was greater for controls (5/47 dogs, 11%) vs the combined presumptive and confirmed CE dogs (0/48 dogs, 0%; P = .05). Fewer dogs with confirmed CE were fed a main diet containing red meat as the primary protein source (2/23 dogs, 9%) vs controls (15/47 dogs, 32%; P = .03). A main diet moisture percentage of ≤14% as fed was significantly associated with confirmed CE in logistic regression analysis (OR 5.71 [95% CI: 1.18-27.69]; P = .03). The presence of dietary carbohydrate, protein source, and dietary moisture content, or factors related to moisture content such as preservatives, might play a role as potential pre-illness dietary risk factors in dogs with CE.

Goats and their role in climate change

Goats are the primary source of protein for many rural communities worldwide, particularly those living in extreme geographical conditions. Goats have numerous advantages that enable them to maintain their production under extreme climate conditions. They have efficient browsing behavior as well as an efficient digestive system, allowing them to remain productive in difficult environmental circumstances. Strong heat resistance, outstanding feed conversion efficiency, survival in adverse climatic circumstances, and high resistance to many diseases are all traits that contribute to production. Because of these features, goat breeding is vital to minimizing and adapting to the negative consequences of climate change. Livestock production accounts for around 7–18% of anthropogenic greenhouse gas emissions. Of this, goats specifically account for 4% of overall greenhouse gas emissions in global animal farming. Enteric CH4 released during production is considered a nutritional energy loss in goats and it’s an important role in global warming and the greenhouse effect. These effects ensure that CH4 production is reduced to provide an optimum economic return from goat farming as well as reduce the impact on global warming. The capacity of goats to adapt to the negative impacts of climate change and their benefits in sustainable production compared to other ruminant species will be thoroughly examined in this paper. As a result, it will contribute to a better understanding of the benefits of goat breeding in the face of future climate change.