Abstract Few lake-wide, seasonal primary production measurements have been made in Lake Erie. None more recent than 1997 have been reported in the literature. In 2019 we used 13C uptake to measure production at 11 stations across the lake during three surveys in May, July, and September and at four of these stations during two additional surveys in April and August. Samples were collected at two depths (2 m and 6 m) and incubated on deck using eight levels of irradiance. We fit the resultant photosynthesis-irradiance curves with models that included the initial slope at low light levels, the maximum photosynthesis rate at light saturation, and when warranted, the negative slope at high irradiance. During May, there was little variation in chlorophyll-normalized volumetric productivity with sample depth, and productivity generally was higher nearshore and in the western basin than it was offshore. The distinction between nearshore and offshore stations was less pronounced in July, but nearshore productivity was higher than offshore in September. Using the photosynthesis data from all five surveys, chlorophyll concentration profiles, and estimated clear-sky solar insolation, we calculated time series of vertically integrated daily production at a western basin station, two central basin stations, and an eastern basin station. Central basin production was highest during August, estimated to be 748 mg C m-2 d-1 and 779 mg C m-2 d-1 at our two stations. Production peaked in July in the western basin (510 mg C m-2 d-1) and September in the eastern basin (421 mg C m-2 d-1). Whole-lake seasonal areal phytoplankton photosynthesis was estimated as 1.661E6 tonnes, about half of estimates made in the 1990s using 14C methods. These results inform our understanding of spatial-temporal trends in Lake Erie productivity and highlight the need for continued measurements of Lake Erie primary production.