The magmatic-hydrothermal evolution of porphyry-style mineralization in the shallow crust that is linked to magmatic processes at depth has been extensively studied using bulk-sample isotopic analysis combined with relative timing constraints. However, a lack of evaluation of the fluid evolution process against an absolute time frame limits further understanding of the ore-forming process. Here, we quantify the fluid evolution process within an absolute time frame for the first time by integrating new in situ oxygen isotope data from the Qulong porphyry Cu-Mo deposit (Tibet) with existing fluid inclusion data and high-precision Re-Os dates of co-precipitated hydrothermal quartz and molybdenite, respectively. We demonstrate that vein quartz records primary oxygen isotopic compositions and reached oxygen isotope equilibrium with ore-forming fluids, and therefore is an archive of the isotopic composition and source of the ore-forming fluids. The δ18Oquartz and δ18Ofluid values, in absolute time, show periodic fluctuations that indicate the presence of three intermittent pulses of magmatic fluid flux, which have been balanced by meteoric water. As such, the flux of magmatic fluid during ore formation was pulsed, rather than being continuous. The overall highest δ18Ofluid in the first pulse of mineralization, with a gradual decrease to the second and third pulses, is suggestive of a progressive reduction in the magmatic component of the hydrothermal fluids and, by inference, the mineralizing potential of the hydrothermal fluids. This view is supported by a decrease in sulfide-bearing fluid inclusions and metal grade through time. Our findings favor multiple fluid-release events from a single cooling magmatic reservoir, although multiple fluid-melt recharge events remain a competitive alternative. An additional implication is that the magmatic reservoir may have a lifespan of hundreds of thousands of years, with fluid release events occurring over tens of thousands of years.