Primary Food Source Research Articles

Ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological profile of Morus alba L.: A comprehensive review

Morus alba L. is a member of family Moraceae that have been traditionally used to cure various ailments in many Asian, South African and European countries. The plant has significant economic value due to its sweet and nutritious fruits which are used in various culinary applications. In addition, the leaves of this plant serve as the primary food source for silkworms, making it a crucial component of the global silk industry. Furthermore, the plant has a long history of use in traditional medicine, and its leaves, bark, and fruit have been utilized for their various therapeutic properties. Morus alba has been extensively studied for its phytochemistry, ethnopharmacology and toxicological profile, with many studies showing promising results. However, despite the growing interest in this plant, there are very few extensive reviews on this topic. The current review provides a comprehensive summary of the distribution, traditional and ethnomedicinal uses, phytochemistry, pharmacological activities and active chemical constituents of M. alba. This study includes distribution and ethnomedicinal use of this plant in different regions of India, explored the phytochemicals, their structure and pharmacological significance. Furthermore, a comparative analysis and investigation revealed the potential of this plant for treating cancer, diabetes, inflammation, hepatoprotection, immunomodulation, antimicrobial properties, and potential for fighting coronavirus. Though, detailed studies are required on the clinical use of M. alba against human diseases. Additionally, the present study revealed that a thorough toxicological investigation must be carried out to ensure its safe and effective use as a health-promoting medicinal plant.

Enhancing Household Food Security and Dietary Diversity through Homegardens: A Case Study in Selected Districts of Sri Lanka

Homegardens are resourceful ecosystems that provide essential services to the environment and mankind. Their contribution to secure household food requirement is among the provisional services of contemporary relevance. A study was conducted in 429 homegardens in Jaffna, Matale, Ratnapura, and Hambantota districts to assess the scope of utilizing homegarden resources on household food security. A questionnaire survey was conducted to gather information on the consumption frequency and the number of food categories that were purchased, produced, and shared. The mean food consumption score (FCS) was 60±23, where 89% of the study population had an acceptable level of FCS (FCS>35). As per the dietary diversity score (HDDS), an average household consumed at least eight food categories per day. Coconut was the most common food commodity produced in homegardens, followed by jackfruit and other fruits (mango, guava, soursop and pomegranate). Households rely on the market-available foods as their primary food source. The study revealed that utilizing at least one food category as a homestead-resource produced, could improve FCS and assist to fulfil the household food demand significantly. However, the majority of the homestead-resources were identified as plant-based, except in Jaffna where the presence of livestock and poultry in homegarden was common. Many of the plant-based resources were from perennial fruit-bearing trees. Therefore, modifying and utilizing the tree composition and viable crops can be considered a timely option to avert household food insecurity. Further, incorporating livestock components (dairy, poultry) as a part of homegardens is beneficial in fulfilling the animal protein demand of the household.

Production and Growth Performance of Nannochloropsis oculata (Droop) Hibberd in the Helical Photobioreactor

Phytoplankton species preferred in marine fish farming are the primary food source in the larval and young stages of bivalve mollusks, along with some fish and shellfish larvae. For this reason, selecting the appropriate phytoplankton and production technique for feeding marine fish larvae is crucial. Nannochloripsis oculata microalgae species, widely used in rearing sea bream and sea bass larvae and feeding rotifers, has an essential place in our country. N. oculata microalgae species, which is used in rearing sea bream and sea bass larvae and feeding rotifers, was produced in a helical photobioreactor by applying a continuous production technique at 30 ‰ salinity, and its growth performance was aimed to be investigated. In the study, N. oculata was grown in a helical photobioreactor at 30‰ salinity by applying the continuous production technique for two weeks, and the daily cell number and specific growth rate were determined. The study was repeated three times. The supply of live feed for marine fish fry and hatcheries established for this purpose is one of the most critical problems. The latest technology in the production of microalgae, which is the primary link of the live feed chain in this sector, is continuous algae production in helical tubular reactors. The helical tubular reactor used in the study consists of a transparent hose system consisting of a completely tubular helical reactor designed for microalgae production. The helical tubular continuous production system offers more efficient and economically more convenient alternatives than existing production systems. One of the most essential advantages of this system is that the system can be designed without requiring significant capital. The continuous culture technique allows the application of long-term and safe algae cultures. With this system, besides providing continuity in production, the negativities in batch production can be eliminated with adequate light. Since the algae production with the continuous method in the spiral photobioreactor remains in the maximum growth phase for a longer time than the batch production, it is a system with high production efficiency and quality, and production capacity is guaranteed. In addition, the algae product harvesting in the continuous system ensures the daily harvesting of algae as much as half of the system volume. A helical reactor enables automation in algae production and high-quality production over long periods. In the study, the sowing density of N. oculata was determined as 1.068x106 ±0.006 cells/mL. Algin cell growth increased logarithmically during the first five days. From the 5th day to the 15th day, the increase in the number of cells was determined as 12.35x106±0.165 cells/mL at the highest and 8.76x106±0.709 cells/mL at the lowest. However, on the 13th day, it was determined that the increase in algin cells was 12.35x106±0.165 cells/mL, and the specific growth rate decreased by 0.189±0.016 divisions/day. As a result of the study, the number of cells of N. oculata algae 5-15 increased. It was determined that it increased 10 times between days. In this context, it was possible to harvest half of the reactor volume of algae daily at maximum cell density for long periods in the helical reactor

Real-life experiments in supermarkets to encourage healthy dietary-related behaviours: opportunities, challenges and lessons learned

BackgroundSupermarkets are the primary source of food for many people yet their full potential as a setting to encourage healthy dietary-related behaviours remains underutilised. Sharing the experiences from research groups who have worked with supermarket chains to evaluate strategies that promote healthy eating could improve the efficiency of building such relationships and enhance the design quality of future research studies.MethodsA collective case study approach was used to synthesise experiences of engaging and sustaining research collaborations with national supermarket chains to test the effectiveness of health-focused in-store interventions. The collective narrative covers studies conducted in three high-income countries: Australia, the Netherlands and the United Kingdom.ResultsWe have distilled our experiences and lessons learned into six recommendations for conducting high quality public health research with commercial supermarket chains. These include: (i) using personal contacts, knowledge of supermarket activities and engaging executive management to establish a partnership and allowing time to build trust; (ii) using scientifically robust study designs with appropriate sample size calculations; (iii) formalising data exchange arrangements and allocating adequate resource for data extraction and re-categorisation; (iv) assessing effects at individual/households level where possible; (v) designing a mixed-methods process evaluation to measure intervention fidelity, dose and unintended consequences; and (vi) ensuring scientific independence through formal contract agreements.ConclusionsOur collective experiences of working in non-financial partnerships with national supermarket chains could be useful for other research groups looking to develop and implement supermarket studies in an efficient manner. Further evidence from real-life supermarket interventions is necessary to identify sustainable strategies that can improve population diet and maintain necessary commercial outcomes.

Crop Disease Detection using Machine Learning

Agriculture is the primary source of food for the world's population, despite the rapid increase in population. Early detection of plant diseases in the field would be beneficial to improve crop production efficiency. Technology has become increasingly important in agriculture in recent years, as it is used to improve efficiency, reduce costs, and increase yields. The emergence of accurate techniques in the field of leaf-based image classification has shown impressive results. Our proposed work includes various phases of implementation of image classification, namely dataset creation, feature extraction, training the classifier, and classification. The work also included hardware design and implementation, as well as software programming for the microcontroller unit of the detector. The system utilized the microcontroller to receive and send data from the various sensors to an online database.

Soybean Collect Recommender Based on Distance and Productivity Cluster Using K-means Clustering and Simple Addictive Weighting Method

Soybeans are an essential agricultural product that is one of the primary food sources in Indonesia, such as tempeh, tofu, soy milk, soy sauce, and other preparations. However, production yields, harvested land area, and soybean productivity in each district or city in Central Java Province vary widely. Differences in soybean productivity in each area are due to production factors such as area, use of fertilizers, seeds, and labor. This study tries to provide recommendations for soybean harvesting based on the distance and productivity of an area using K-means clustering and the simple addictive weighting method. In the Central Java Province, 35 regions will be divided into four clusters: the first with high productivity, the second with medium productivity; the third with low productivity; and the fourth with very low productivity. Additionally, based on the fourth cluster clustering results, it will be advised to take soybeans from other clusters by taking the closest distance and cluster members into account. According to the research, four clusters have formed: the first has five members, the second has fourteen, the third has nine, and the fourth has seven. The fourth cluster, which consists of seven members who do not grow soybeans, is advised to buy soybeans from the following regions: Kendal Regency, Klaten Regency, Magelang Regency, Batang Regency, and Brebes Regency.

Synchrony in whitefish stock dynamics: disentangling the effects of local drivers and climate

Synchronic variations in abundance in populations of the same species are common phenomena encountered in various environments, including lakes, and different taxa of freshwater fishes. This phenomenon can be caused by similar environmental conditions across physically separated populations. In the context of the ongoing climate change, it is essential to test this hypothesis, identify the factors driving the synchrony and elucidate the mechanisms, in the attempt to improve fisheries management. This study investigates synchronic variations in European whitefish (Coregonus spp.) populations in five peri-alpine lakes. The hypothesis suggests that shared biotic or abiotic factors contribute to similar trends in whitefish landings. Environmental and seasonal variables impacting the early life stages of the species were analyzed, and the Euclidean distances between the multivariate time series were calculated to identify similarities or dissimilarities in lake environmental parameters. We found that regional winter and spring temperatures were consistent across the lakes, but these factors did not fully account for variations in landings statistics. Wind intensity, water level and zooplankton abundance showed lake-specific patterns that could better explain local conditions and dynamics. Linear models did not reveal a coherent correlation with a common environmental variable across all lakes. However, distinct relationships were found in four of the lakes, with local factors significantly contributing to abundance variations. The spring abundance of Daphnia spp., a primary food source for whitefish larvae, was the main factor correlated with fish landing trends in Lake Geneva and Lake Bourget. Higher availability of Daphnia spp. may decrease intraspecific competition and density-dependent mortality. In Lake Neuchâtel, winter water temperature was negatively correlated with fish abundance proxies, suggesting that warmer winters may compromise reproduction success. Lake Annecy saw an increase in whitefish landings following a substantial reduction in fishing efforts during the late 2000s. A significant negative correlation was found between whitefish landings and fishing efforts. No relationship was found for Lake Aiguebelette, maybe due to a lack of zooplankton data. In conclusion, the observed synchrony in the European whitefish population is likely driven by a combination of interacting environmental and anthropogenic factors rather than a single common variable. Further research and a more detailed dataset are needed to better understand these complex relationships. Cover image: Whitefish (courtesy of Rémi Masson)

Indonesian market demand patterns for food commodity sources of carbohydrates in facing the global food crisis

Global food consumption tends to rise more quickly than supply. This has to do with important global issues like population growth. Additionally, global conflicts are going to hinder the distribution of food. Indonesia has an enormous opportunity of anticipating these circumstances considering its promise as one of the largest supplies of food worldwide. Rice is still the staple food in Indonesia, but the dynamics of society are under threat from wheat food. It is possible to create strategy plans to deal with potential food scarcity by understanding the behavior of food demand trends for big carbohydrate sources like corn, cassava, sweet potatoes (as a substitution), and the development of wheat as a beneficial food. The results of the study indicate that rice, corn, cassava, and sweet potatoes—food commodities that are major sources of carbohydrates—are inelastic, which means that their prices are not affected by variations in demand. The community still relies on rice as the primary food source. Cross elasticity >0 in these non-wheat food commodities indicates mutually beneficial replacement among the foods that are sources of carbohydrates. That is, with the dynamics of an increase in income, for example, it will also increase consumption. The results of the study also demonstrate that wheat food items are only a complementary, not a staple food needed, thus concerns about wheat's dominance as a food component in industrial products actually have no impact on local food. The availability of high-yielding varieties of rice, corn, cassava, and sweet potatoes, the implementation of food reserves by the Indonesian National Logistics Agency (Bulog) from the government center to the regions, food diversification, changing preferences, and creating an awareness of local food pride with massive education are some of the anticipatory steps taken in response to the global food crisis.

Deep CNN-Based Planthopper Classification Using a High-Density Image Dataset

Rice serves as the primary food source for nearly half of the global population, with Asia accounting for approximately 90% of rice production worldwide. However, rice farming faces significant losses due to pest attacks. To prevent pest infestations, it is crucial to apply appropriate pesticides specific to the type of pest in the field. Traditionally, pest identification and counting have been performed manually using sticky light traps, but this process is time-consuming. In this study, a machine vision system was developed using a dataset of 7328 high-density images (1229 pixels per centimetre) of planthoppers collected in the field using sticky light traps. The dataset included four planthopper classes: brown planthopper (BPH), green leafhopper (GLH), white-backed planthopper (WBPH), and zigzag leafhopper (ZIGZAG). Five deep CNN models—ResNet-50, ResNet-101, ResNet-152, VGG-16, and VGG-19—were applied and tuned to classify the planthopper species. The experimental results indicated that the ResNet-50 model performed the best overall, achieving average values of 97.28% for accuracy, 92.05% for precision, 94.47% for recall, and 93.07% for the F1-score. In conclusion, this study successfully classified planthopper classes with excellent performance by utilising deep CNN architectures on a high-density image dataset. This capability has the potential to serve as a tool for classifying and counting planthopper samples collected using light traps.

Fish on Fire: Shifts in Amazonian fish communities after floodplain forest fires

Abstract Severe droughts can lead to fires that cause massive tree mortality in even the wettest and most isolated Amazonian forests. After repeated fires, blackwater floodplain forests can remain in an open vegetation state of arrested succession that facilitates a transition towards a white‐sand ‘savanna‐like’ vegetation. These vegetation shifts, from closed‐canopy floodplain forests to open fire scars and eventually white‐sand savannas, may have profound implications for fish communities that depend on floodplain forests for food and recruitment. In turn, changes in fish communities may contribute to the arrested forest recovery after fires as fish are important seed dispersal agents for many floodplain tree species. To explore the impacts of floodplain forest fires on fish communities, we sampled fish in unburnt forests, fire scars, and white‐sand savannas in the middle Rio Negro basin (Brazil) during two consecutive flooding seasons. We compared the abundance, species richness, and the taxonomic and trophic composition of fish assemblages across the three habitat types. We found significant shifts in fish assemblages in fire scars compared to unburnt floodplain forests. Also, as fire scars increased in size, total fish biomass decreased strongly. Fish communities in unburnt floodplain forests seem to be characterized by a higher proportion of smaller and omnivorous fish species than fish communities in burned floodplain forests and white sand savannas. Fish assemblages in fire scars and white‐sand savanna were not significantly different. Synthesis and Applications. Amazonian fish communities change after floodplain forest fires. Unburnt forests have diverse fish communities, with a large proportion of unique and small omnivorous species. In contrast, carnivores and detritivores become more common in fire scars and white‐sand savannas formed after forest fires. Less omnivore fish after forest fires can reduce tree seed dispersal and forest regeneration, affecting ecosystem functioning and the services provided by Amazonian forests. Fish are also a primary source of food and income for people living in Amazonian floodplains. Preventing forest fires is therefore of fundamental importance for the conservation of Amazonian aquatic and terrestrial ecological communities as well as for the livelihood of people.

Identification of qPSR7-2 as a Novel Cold Tolerance-Related QTL in Rice Seedlings on the Basis of a GWAS

Rice is the primary source of food for more than half of the global population. Accordingly, improving the cold tolerance of rice is vital for ensuring food security. In this study, a new cold tolerance-related QTL in rice (qPSR7-2) was detected on chromosome 7 following a genome-wide association study involving 173 japonica rice germplasm resources. The fine mapping of this locus identified Os07g0541800 as a candidate gene associated with qPSR7-2. This gene encodes a cysteine-rich receptor-like kinase. The functional verification of Os07g0541800 involving transgenic plants indicated that qPSR7-2 positively regulates rice cold tolerance at the seedling stage. The examination of the cold tolerance of 984 germplasm resources from the 3000 Rice Genomes Project at the seedling stage and their respective haplotypes at qPSR7-2 revealed that the proportion of favorable haplotypes in germplasm resources increased as the latitude increased. More than 90% of the rice varieties cultivated in Europe and Japan appear to carry qPSR7-2, implying that qPSR7-2 may mediate the acclimation of rice to low-temperature stress. The findings of this study will further clarify the molecular networks regulating rice cold tolerance, while also providing researchers and breeders with new genetic resources and information relevant for developing cold-tolerant rice varieties.

Negative Impacts of Trace Metal Contamination on the Macrobenthic Communities along the Santos Port Complex—Brazil

Port sites represent one of the most impacted coastal areas; this impact is due to intensive anthropogenic pressures. In addition to the port complex itself, associated activities, such as indiscriminate disposal of pollutants, including trace metals, affect the local ecosystem. Macroinvertebrate benthic communities are one of the most effective bioindicators of environmental health because of their importance as a primary food source for many fish, birds, and mammals, as well as their influence on sediment stability and geochemical composition. This article evaluates the benthic macrofauna in the Santos Estuarine System (SES), the location of the Santos Port Complex (SPC), linking trace metal levels to differences in microbenthic community structure and pollutant bioavailability. The distribution of Cd, Ni, and Pb was directly related to organic matter deposits, while Cu and Zn appeared to result from port activities. The SES contained a poor benthic macroinvertebrate community, resulting from the contaminated muddy sediments. A significant negative correlation was found between the macrobenthic diversity and concentrations of Cu in the soluble phase; this implied the pollution-induced degradation of the macrobenthos in SES.

Innova 2020: A Follow-Up Study of the Fecal Microbiota of Infants Using a Novel Infant Formula between 6 Months and 12 Months of Age.

The World Health Organization recommends exclusive breastfeeding on demand until at least the sixth month of life. Breast milk or infant formula is the infant's primary food source until the age of one year, followed by the gradual introduction of other foods. During weaning, the intestinal microbiota evolves to a profile close to that of the adult, and its disruption can result in an increased incidence of acute infectious diseases. We aimed to determine whether a novel starting formula (INN) provides gut microbiota compositions more similar to those of breastfed (BF) infants from 6 to 12 months of age compared to a standard formula (STD). This study included 210 infants (70 per group) who completed the intervention until they reached the age of 12 months. In the intervention period, infants were divided into three groups. Group 1 received an INN formula with a lower protein content, a casein to whey protein ratio of approximately 70/30, twice as much docosahexaenoic acid as the STD formula, a thermally inactivated postbiotic (Bifidobacterium animalis subsp. lactis, BPL1TM HT), and twice as much arachidonic acid as the STD formula contained. The second group received the STD formula, while the third group was exclusively BF for exploratory purposes. In the course of the study, visits were conducted at 6 months and 12 months of age. Compared to the BF and STD groups, the Bacillota phylum levels in the INN group were significantly reduced after 6 months. At the end of 6 months, the alpha diversity indices of the BF and INN groups differed significantly from those of the STD group. At 12 months, the Verrucomicrobiota phylum levels in the STD group were significantly lower than those in the BF and INN groups. Based on the comparison between 6 and 12 months, the Bacteroidota phylum levels in the BF group were significantly higher than those in the INN and STD groups. When comparing the INN group with the BF and STD groups, Clostridium sensu stricto 1 was significantly higher in the INN group. The STD group had higher levels of calprotectin than the INN and BF groups at 6 months. The immunoglobulin A levels in the STD group were significantly lower than those in the INN and BF groups after 6 months. Both formulas had significantly higher levels of propionic acid than the BF group at 6 months. At 6 months, the STD group showed a higher quantification of all metabolic pathways than the BF group. The INN formula group exhibited similar behavior to the BF group, except for the superpathway of phospholipid biosynthesis (E. coli). We hypothesize that the novel INN formula may promote an intestinal microbiota that is more similar to the microbiota of an infant who consumes only human milk before the weaning period.

Characterization of pearl millet [Pennisetumglaucum (l.) R. Br.] drought tolerant lines developed for A1 zone using SSR markers

Pearl millet [Pennisetum glaucum (L.) R. Br] is the fifth most important cereal in the world and is a primary food source of arid and semi-arid tropical regions of Asia and Africa. It is mainly grown over 30 million ha worldwide with majority of the crop grown in Africa and Asia. It is very useful for combating the adverse effects of changing climate and can also ensure livelihood security among farming communities. It has climate-resilient attributes and can grow in less fertile soil even under adverse and harsh climatic conditions. It can show better growth and productivity in low nutrient input conditions and needs lesser synthetic fertilizers and irrigations. Screening and selecting germplasm for drought tolerance and developing drought tolerant hybrids and varieties of pearl millet is very important in the present scenario of global warming and climatic change. In this study, 24 drought tolerant lines of pearl millet developed specifically for A1 zone were characterized using 15 drought specific SSR primers. All the 15 SSRs amplified products of varying sizes ranging from 100-550 bp. A total of 38 alleles were obtained in this study and the number of alleles per locus varied from 2 to 4 with an average of 2.53 alleles. Polymorphic Information Content (PIC) varied from 0.41 to 0.69 with an average of 0.51 PIC value. This study can be highly useful for developing high yielding drought tolerant cultivars for low rainfall areas i.e. A1 zone and increasing pearl millet productivity.&nbsp

Effects of Climate Change, Land Use and Land Cover Variability on Green and Blue Water in Wami/Ruvu Basin, Tanzania

Water basins are the primary food sources, giving green water and blue water worldwide. Despite the basins’ potential, information on the periodical variations in blue water and green water is sparse, particularly in developing countries. The study specifically evaluated the changes in land use and land cover variability (LULCV), effects of land use and land cover variability on green water and blue water variations, and effects of climatic changes on green water and blue water. The evaluation involved the Enhanced Thematic Mapper and Operational Land Imager satellite images of 1990, 2000, 2010 and 2020. Image processing utilized the Soil and Water Assessment Tool (SWAT) in ArcGIS software. The land use and land cover variabilities indicated that land use supporting social-economic activities increased, while natural land cover decreased. Proportionally, blue water per annum was decreasing due to declining natural vegetation, enhanced by the increased socio-economic activities. Whereas, the increase in green water per annum was due to the increased temperatures, boosted by climate changes. Since the temperature rise is mainly uncontrolled, greenhouse farming should be encouraged for making green water more productive in agriculture and communities should be encouraged to practice environmentally friendly anthropogenic activities for sustainable green water and blue water management.
 Keywords: Basin, Green water, Blue water, SWAT model, Climate change

Occurrence of the Red-bellied Snake (Storeria occipitomaculata) on the margins of a disjunct range

The Red-bellied Snake (Storeria occipitomaculata) can be found throughout much of eastern North America, though two notable disjunct populations exist in the Black Hills of southwestern South Dakota and the Central Platte River Valley in southcentral Nebraska, west of the species’ core range. While literature continues to be added on the Red-bellied Snake and its natural history, it remains largely understudied in Nebraska. Throughout much of this species’ range, it is considered associated with woodland and wetland habitat features. On 28 September 2021, we detected one Red-bellied Snake near a treeless playa wetland in the Rainwater Basins ecoregion of southcentral Nebraska. Using a Grubbs Test to determine significant outliers, we found that this record was further from the Platte River than other previous accounts of this species in the state. This observation suggests this species may be found in other portions of the Rainwater Basins and considerably further from the Platte River than previously believed. Our observation along with other recent work indicates that Red-bellied Snakes may persist in herbaceous habitats lacking established woodland that include sufficient wetland features to support their primary food sources, including gastropods and other soft-bodied invertebrates. Due to the fragmentation of suitable habitats in the Rainwater Basins, coupled with the low dispersal of Red-bellied Snakes, populations persisting in the region are likely to be isolated.

Mediterranean juvenile white seabream rely on phytal fauna as primary food source in coastal nursery areas

Nursery areas are essential fish habitats due to their relevance in the survival of early stages of fish populations. They are also considered as of high priority in marine conservation strategies. Here, we investigated the diet of white seabream [Diplodussargus (Linnaeus, 1758)] settlers in six nursery areas located in the shallow waters of coves in Minorca Island (Balearic Islands, Spain). Our aim was to characterize the food sources at different stages of juvenile development and their site-related variability in order to discern the importance of trophic links in cove selection during settling. The gut contents of 101 juveniles captured at different coves, three to the north of the island (N), and three in the southern coast (S), revealed a marked preference for feeding on crustaceans and, in particular, on harpacticoid copepods (>90% of gut contents). Copepods represented the main food source (80 ±4.4%; mean ±S.E.) in younger seabream individuals (10 - 15 mm length). A higher diversity in prey items was observed in the larger size-classes (s2: 16 - 23 and S3: 24 - 30 mm, respectively), which incorporated other prey items such as amphipods, isopods, foraminiferans or ostracods. Diet composition did not vary between the two surveyed locations (North vs. South of the island), but it did show significant differences among the six coves (p<0.001). Comparison between cove sediment infaunal composition and gut contents revealed that predation on sediment communities was scarce. Instead, diet was typically of phytal origin. Our results highlight the importance of the algal component of shallow coastal areas as a foraging habitat. In particular, harpacticoid copepods were key for survival during early development phases. The potential use of harpacticoid copepods to track ontogenic shifts in habitat use by juvenile fish is discussed.

High yielding mulberry production through controlled pollination for enhanced vegetative growth and early sprouting suitable for tropical agroclimatic regions

Mulberry is an important crop for the sericulture sector because they are the main source of nutrition for silkworms. Sericulture is the practice of raising silkworms to produce silk, which is a valuable and opulent textile in many industries. Mulberry is the primary food source for silkworms, hence the availability and quality of mulberry are crucial to the sericulture industry. Therefore, development of improved varieties is essential for enhancing silk production. Attempts were made to develop high yielding mulberry varieties with vigorous vegetative growth and early sprouting suitable for tropical agroclimatic regions through controlled pollination. The importance of developing a high-yielding variety with early sprouting is emphasized, as it can lead to increased silk production with improved profitability to farmers. The review also describes methods of controlled pollination, which are used to produce hybrid seeds with desired traits. The various factors that influence the success of controlled pollination, including timing, temperature, humidity, and nutrition, are discussed. Finally, the review summarizes the research on the development of high-yielding mulberry varieties with vigorous vegetative growth and early sprouting. Overall, the information provides valuable insights into the development of improved mulberry varieties for tropical regions and highlights the potential for increased silk production and profitability to farmers.

Real-Time Intracellular Analysis of Kanamycin Using Microaptasensors.

With the emergence of multidrug-resistant bacteria, infection-related death toll is on the rise. Overuse of antibiotics and their leakage into waterways have transformed the environment into a sink, resulting in bacterial resistance permeating through all tiers of the food cycle. As one of the primary sources of food, fish and fish products such as fish eggs must be studied for their ability to accumulate relevant antibiotics. While the accumulation of these pharmaceuticals has previously been studied, there remains a need to analyze these processes in real time. Electrochemical aptamer-based sensor technology allows for selective, real-time monitoring of small molecules. Herein, we report the first use of miniaturized electrochemical aptamer-based sensors for the analysis of the passive uptake of the aminoglycoside antibiotic, kanamycin, in single salmon eggs. We use pulled platinum microelectrodes and increase the surface area at the electrode tip through dendritic gold deposition. These electrodes showed a 100-fold increase in DNA immobilization on the electrode surface as compared to bare microelectrodes. Additionally, the sensors showed improved stability in complex biological media over an extended period of time when compared to the more widely used macrosensors (r = 1 mm). The sensor range was determined to extend from nanomolar to micromolar concentrations of kanamycin in fish egg lysate and when used in a single salmon egg the μ-aptasensors were able to monitor the passive uptake of kanamycin over time. The accumulation kinetics were simulated using COMSOL Multiphysics software. This research presents the first reported record of passive uptake of a small molecule in a single cell in real-time using electrochemistry.

Bioactive Oxylipins Profile in Marine Microalgae

Microalgae are photosynthetic microscopic organisms that serve as the primary food source in aquatic environments. Microalgae can synthesize a wide variety of molecules, such as polyunsaturated fatty acids (PUFAs) of the omega-3 and omega-6 series. Oxidative degradation of PUFA due to radical and/or enzymatic conversion leads to the formation of oxylipins, which are compounds known for their bioactive properties. In the present study, we aim to profile oxylipins from five microalgae species grown in 10-L photo-bioreactors under optimal conditions. During their exponential phase, microalgae were harvested, extracted and analyzed by LC-MS/MS to determine the qualitative and quantitative profile of oxylipins for each species. The five different selected microalgae revealed a high diversity of metabolites, up to 33 non-enzymatic and 24 enzymatic oxylipins present in different concentrations. Taken together, these findings highlight an interesting role of marine microalgae as a source of bioactive lipids mediators, which we hypothesize have an important function in preventive health measures such as amelioration of inflammation. The rich mixture of oxylipins may display advantages to biological organisms, especially by providing for human health benefits including antioxidant, anti-inflammatory, neuroprotective or immunomodulator activities. Some oxylipins are also well known for their cardiovascular properties.