This paper characterized a new fixed rope media system to upgrade decentralized and small-scale wastewater treatment plants. A primary effluent of municipal wastewater was treated using two pilot-scale reactors equipped with full-scale sized fixed rope media technology, one of the reactors was aerated using a coarse bubble tube and the other using a custom fine bubble aeration system. The study examined the impact of the aeration scheme and intensities and the COD/NH 3 -N ratio on ammonia and COD removal rates, excessive biofilm growth, slough-off, and microbial communities’ composition. The average biofilm ammonia and COD removal rates ranged from 0.23 ± 0.15 to 0.38 ± 0.26 gNH 3 -N/m 2 .d and 1.35 ± 0.95 to 3.05 ± 1.21 gCOD/m 2 .d, respectively. The fine and coarse bubble reactors showed comparable carbon oxidation rates; however, the fine bubble reactor showed a higher nitrification rate than the coarse bubble reactor at lower aeration intensities despite the similar dissolved oxygen concentration. Correspondingly, an increase in COD/NH 3 -N and excessive biofilm growth decreased the NH 3 -N removal performance but did not affect the COD removal efficiency. Further analysis of the microbial communities composition revealed that the reactors supported a relatively substantial amount of AOB (55 and 63%) and denitrifying bacteria (36 and 21%) with a relatively lower NOB (7 and 8%) and anammox (1 and 8%) species in the fine and coarse bubble aeration reactors, respectively. Overall this study demonstrated the feasibility of one stage fixed rope media to treat COD and ammonia and meet treatment objectives, thus providing an alternative solution to decentralized and smaller plant upgrades. • Ammonia and COD removal efficiency of innovative fixed rope media technology was evaluated. • Different aeration schemes showed minimal impact on the DO concentration but impacted removal performances. • C/N ratio influenced NH 3 -N removal but not COD removal. • Slough off did not impact COD removal; however, it enhanced ammonia removal. • Reactor with the fine bubble aeration supported a relatively higher fraction of denitrifying bacteria.