Goal. To study the cytotoxicity of certain antibacterial preparations used in the treatment of infectious diseases of the urinary system, as well as some disinfectants for calf kidney cell culture. Methods. Cytological, toxic effects test on primary calf kidney cell culture. Determination of the maximum permissible concentrations of antibiotics, antiseptics, and disinfectants that do not yet lead to degeneration of the cell monolayer of calf kidney cells. Results. Tests of some drugs that are often used in veterinary medicine show that the least toxic to calf kidney cells of all tested antibacterial agents are Oxymicol 20 (active substance — oxytetracycline base), Norsulfazole sodium, and Farmazin 50 (active substance — tylosin). Conclusions. These drugs or similar with the same active substances should be used in the treatment of infectious diseases of the kidney and urinary system of cattle and other animals. The possibility of using the method of cultivation and use as test objects of primary cultures of eukaryotic cells in the selection of the optimal composition of therapeutic and prophylactic drugs or disinfectants for veterinary medicine. The obtained results of cytotoxicity can help veterinarians in choosing antimicrobials and disinfectants in terms of their wide range on the market.