Abstract
Goal. To study the cytotoxicity of certain antibacterial preparations used in the treatment of infectious diseases of the urinary system, as well as some disinfectants for calf kidney cell culture. Methods. Cytological, toxic effects test on primary calf kidney cell culture. Determination of the maximum permissible concentrations of antibiotics, antiseptics, and disinfectants that do not yet lead to degeneration of the cell monolayer of calf kidney cells. Results. Tests of some drugs that are often used in veterinary medicine show that the least toxic to calf kidney cells of all tested antibacterial agents are Oxymicol 20 (active substance — oxytetracycline base), Norsulfazole sodium, and Farmazin 50 (active substance — tylosin). Conclusions. These drugs or similar with the same active substances should be used in the treatment of infectious diseases of the kidney and urinary system of cattle and other animals. The possibility of using the method of cultivation and use as test objects of primary cultures of eukaryotic cells in the selection of the optimal composition of therapeutic and prophylactic drugs or disinfectants for veterinary medicine. The obtained results of cytotoxicity can help veterinarians in choosing antimicrobials and disinfectants in terms of their wide range on the market.
Highlights
Norsulfazole sodium Action on the monolayerNo degenerative changes in the monolayer were observed at 96 hours at maximum allowable concentrations (MAC) and lower concentrations in cell cultures, and all cell lines retained the ability to be further cultured
An important issue for veterinary medicine is the choice of optimal and at the same time low-toxic drugs for the treatment of various bacterial infections
The 3rd International Congress on Alternative Methods in the Use of Animals in the Life Sciences initiated the Good Cell Culture Practice (GCCP), a project developed by the European Centre for the Validation of Alternative Methods [1]
Summary
No degenerative changes in the monolayer were observed at 96 hours at MAC and lower concentrations in cell cultures, and all cell lines retained the ability to be further cultured. Concentrations above the MAC after 2448 hours caused degeneration of the monolayer in the cell lines: the cells were rounded, separated from the surface of the wells, the pH of the medium was shifted to the acidic side. Antibiotics amoxicillin and tylosin belong to the moderately dangerous substances (Danger class 3 is in accordance with GOST 12.1.007); they are active substances in the drugs Amoxicillin and Pharmazin 50. Their MAC on calf kidney cell culture differed significantly. The results of cytotoxicity testing of individual disinfectants and antiseptics are given in table 2
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