Observations in 2021–2022 showed that the zooplankton community of the Irtysh River was formed by 33 species and two forms from three systematic groups: rotifers — 19, Cladocerans were represented by 12, copepods by 2 species from the order Cyclopoida and 2 species from the orders Harpactiformes and Calaniformes. The planktofauna of the river is represented by species common to water bodies of Western Siberia. The main part of rotifers belonged to typically planktonic eurythermal species. Among the cladocerans, along with planktonic species, there were also phytophilic and benthic forms. Average zooplankton indicators for the growing season of 2021 reached 3646 specimens/m3 in numbers and 70.7 mg/m3 in biomass. In 2022, the quantitative values were slightly lower: 2237 specimens/m3 and 40.4 mg/m3. Asplanchna priodonta Gosse and Mesocyclops leuckarti Claus were dominating species in spring, Bosmina longirostris O.F. Müller in summer, Alona intermedia Sars and Ceriodaphnia affinis Lillijeborg in autumn of both years accordingly. Waters of the Irtysh River is characterized mainly by the values of indicators corresponding to the α-mesosaprobic zone. The saprobity index in spring was 1.5, in summer and autumn — 1.6, which corresponds to moderately polluted waters. In terms of a set of zooplankton indicators, the water of the Irtysh River at the present stage is somewhat different from the previous low-water period in the beginning of this century. Currently, the number of species has increased, the structure of the community has changed, in which the dominance of branchous crustaceans has been noted, the biomass has significantly increased and the saprobity index has decreased, that is, the purity of water has increased. Qualitative and quantitative indicators of zooplankton, as well as the level of saprobity, are more consistent with the period of the second half of the last century.