Previous Assessments Research Articles

Implementing Adaptive Neuro-Fuzzy Inference Systems (ANFIS) for Risk Assessment of Drug Interactions

Novel drug development is time-consuming, difficult, and often unsuccessful. This has made combining therapies increasingly common and profitable in recent years. Healthcare professionals in the pharmaceutical sector are interested in the combination, but we must instantly solve drug-drug interactions. In few cases, single-perspective DDI evaluations are insufficient. Pharmacological therapy and patient safety depend on accurate drug interaction risk assessments. This work uses an Adaptive Neuro-Fuzzy Inference System (ANFIS) to assess and predict medication interaction hazards. The proposed method uses neural networks' generative skills and fuzzy logic to construct a robust decision-making framework that can handle drug interaction scenarios and their unpredictability. Fuzzy rules provide all the necessary risk assessment components. This includes dose, patient health issues, drug interaction profiles, and pharmacological properties. Accuracy, specificity, and Mean Squared Error evaluate the model after training with known drug interactions. These metrics evaluate model performance. These measures are essential for machine learning evaluation. The ANFIS-based model outperforms previous risk assessment methods in risk classification and prediction. This study found that the ANFIS architecture may improve medication management security and prevent dangerous drug interactions.

Exploring and Modeling Directional Effects on Steering Behavior in Virtual Reality.

Steering is a fundamental task in interactive Virtual Reality (VR) systems. Prior work has demonstrated that movement direction can significantly influence user behavior in the steering task, and different interactive environments (VEs) can lead to various behavioral patterns, such as tablets and PCs. However, its impact on VR environments remains unexplored. Given the widespread use of steering tasks in VEs, including menu adjustment and object manipulation, this work seeks to understand and model the directional effect with a focus on barehand interaction, which is typical in VEs. This paper presents the results of two studies. The first study was conducted to collect behavioral data with four categories: movement time, average movement speed, success rate, and reenter times. According to the results, we examined the effect of movement direction and built the SθModel. We then empirically evaluated the model through the data collected from the first study. The results proved that our proposed model achieved the best performance across all the metrics (r2 > 0.95), with more than 15% improvement over the original Steering Law in terms of prediction accuracy. Next, we further validated the SθModel by another study with the change of device and steering direction. Consistent with previous assessments, the model continues to exhibit optimal performance in both predicting movement time and speed. Finally, based on the results, we formulated design recommendations for steering tasks in VEs to enhance user experience and interaction efficiency.

Quantitative biomechanical analysis in validating a video-based model to remotely assess physical frailty: a potential solution to telehealth and globalized remote-patient monitoring.

Assessing physical frailty (PF) is vital for early risk detection, tailored interventions, preventive care, and efficient healthcare planning. However, traditional PF assessments are often impractical, requiring clinic visits and significant resources. We introduce a video-based frailty meter (vFM) that utilizes machine learning (ML) to assess PF indicators from a 20s exercise, facilitating remote and efficient healthcare planning. This study validates the vFM against a sensor-based frailty meter (sFM) through elbow flexion and extension exercises recorded via webcam and video conferencing app. We developed the vFM using Google's MediaPipe ML model to track elbow motion during a 20s elbow flexion and extension exercise, recorded via a standard webcam. To validate vFM, 65 participants aged 20-85 performed the exercise under single-task and dual-task conditions, the latter including counting backward from a random two-digit number. We analyzed elbow angular velocity to extract frailty indicators-slowness, weakness, rigidity, exhaustion, and unsteadiness-and compared these with sFM results using intraclass correlation coefficient analysis and Bland-Altman plots. The vFM results demonstrated high precision (0.00-7.14%) and low bias (0.00-0.09%), showing excellent agreement with sFM outcomes (ICC(2,1): 0.973-0.999), unaffected by clothing color or environmental factors. The vFM offers a quick, accurate method for remote PF assessment, surpassing previous video-based frailty assessments in accuracy and environmental robustness, particularly in estimating elbow motion as a surrogate for the 'rigidity' phenotype. This innovation simplifies PF assessments for telehealth applications, promising advancements in preventive care and healthcare planning without the need for sensors or specialized infrastructure.

Effect of chloramine T and chlorhexidine in reducing gingivitis in patients admitted to a reference hospital for cardiopulmonary care: a randomized, controlled clinical trial.

The aim of this study is to evaluate the effect of Chloramine T in reducing gingivitis in hospitalized patients, in addition to providing an alternative to the use of Chlorhexidine. A randomized, triple-blind, placebo controlled clinical trial was performed with 45 volunteers presenting with gingivitis, using the Löe Gingival Index (LGI). Patients were randomly allocated into one of three groups: (G1): Chloramine T 0.2%; (G2): Chlorhexidine 0.12% and (G3): distilled water. After five days, the control group (G3) remained without relevant differences in relation to the first pre-treatment assessment, while the groups tested with chlorhexidine and chloramine T showed improvement in the LGI (p < 0.05). After ten days, G3 maintained the results of the previous assessments, G1 repeated the five-day assessment and G2 obtained a statistically significant difference, with a better result in relation to the previous assessment (p < 0.05). After fifteen days, G3 showed no differences in relation to the other assessments, G1 had a statistically significant difference, showing better performance in relation to the previous assessment and G2 continued the gradual improvement it had been showing in the LGI (p < 0.05). Chloramine T and Chlorhexidine present similar results in the treatment of gingivitis, when evaluated by probing by the LGI, and Chloramine T can be used effectively, without causing the adverse effects inherent to prolonged use of chlorhexidine. Importance of oral hygiene for patients admitted to hospitals with the use of adjuvant substances that do not present adverse effects such as chlorhexidine, which is considered the gold standard, seeking to provide better clinical protocols.

Variability of 7K and 11K SomaScan Plasma Proteomics Assays.

SomaScan is an aptamer-based proteomics assay designed for the simultaneous measurement of thousands of human proteins with a broad range of endogenous concentrations. The 7K SomaScan assay has recently been expanded into the new 11K version. Following up on our previous assessment of the 7K assay, here, we expand our work on technical replicates from donors enrolled in the Baltimore Longitudinal Study of Aging. By generating SomaScan data from a second batch of technical replicates in the 7K version as well as additional intra- and interplate replicate measurements in the new 11K version using the same donor samples, this work provides useful precision benchmarks for the SomaScan user community. Beyond updating our previous technical assessment of the 7K assay with increased statistics, here, we estimate interbatch variability, assess inter- and intraplate variability in the new 11K assay, compare the observed variability between the 7K and 11K assays (leveraging the use of overlapping pairs of technical replicates), and explore the potential effects of sample storage time (ranging from 2 to 30 years) in the assays' precision.

Assessment of digital elevation models accuracy for local geoid modeling

One of the critical factors influencing the accuracy of a local geoid model is the quality of the digital elevation model (DEM). A properly selected high-resolution DEM can significantly mitigate errors in geoid modeling, gravity anomaly processing, and topography and downward continuation correction. Purpose. Evaluating the accuracy of five global DEMs obtained from open sources to identify the most suitable model for creating a local geoid. Methodology. The vertical accuracy of the DEMs was assessed by comparing the heights between the DEM and control points across different types of terrain. The reference values are based on 344 ground benchmarks, where GNSS observations were performed with subsequent adjustment of coordinates and heights. The accuracy analysis involved calculating statistical indicators of the height differences between the GNSS data and the DEM data. Findings. The standard deviation assessment showed favorable values for the COPERNICUS and ALOS DEMs, followed by SRTM, ASTER, and ETOPO. In the mean absolute error calculations for mountainous areas, the ALOS model performed best, followed by COPERNICUS, SRTM, ASTER, and ETOPO. For other types of terrain, COPERNICUS demonstrated the best results in mean absolute error. Originality. This study distinguishes itself through the incorporation of advanced high-resolution DEMs, such as GLO30, providing a modern and thorough evaluation of DEM accuracy specifically for Kazakhstan. What is new is a detailed analysis of the impact of terrain features (plain, hilly, mountainous) on modeling accuracy. This approach advances beyond previous assessments, delivering new and significant insights into the performance of contemporary DEMs. Practice value. The practical value of the results obtained consists in issuing recommendations regarding the possibility of using the studied DEM for the regions of Kazakhstan which differ among themselves in terms of landscape characteristics. The findings indicate that COPERNICUS and ALOS DEMs are highly suitable for precise geoid modeling in southern Kazakhstan. These models can significantly improve the accuracy of local geoid models, benefiting applications in geospatial science and engineering.

Aligning the Western Balkans power sectors with the European Green Deal

Abstract Located in Southern Europe, the Drina River Basin is shared between Bosnia and Herzegovina, Montenegro, and Serbia. The power sectors of the three countries have an exceptionally high dependence on coal for power generation. In this paper, we analyse different development pathways for achieving climate neutrality in these countries and explore the potential of variable renewable energy (VRE) and its role in power sector decarbonization. We investigate whether hydro and non-hydro renewables can enable a net-zero transition by 2050 and how VRE might affect the hydropower cascade shared by the three countries. The Open-Source Energy Modelling System (OSeMOSYS) was used to develop a model representation of the countries' power sectors. Findings show that the renewable potential of the countries is a significant 94.4 GW. This potential is 68% higher than previous assessments have shown. Under an Emission Limit scenario assuming net zero by 2050, 17% of this VRE potential is utilized to support the decarbonization of the power sectors. Additional findings show a limited impact of VRE technologies on total power generation output from the hydropower cascade. However, increased solar deployment shifts the operation of the cascade to increased short-term balancing, moving from baseload to more responsive power generation patterns. Prolonged use of thermal power plants is observed under scenarios assuming high wholesale electricity prices, leading to increased emissions. Results from scenarios with low cost of electricity trade suggest power sector developments that lead to decreased energy security.

Calcium-Binding Protein and Polymorphism in Musa spp. Somaclones Resistant to Fusarium oxysporum

The fresh fruits of ‘Grande Naine’ (Cavendish AAA—Musa spp.) dominate the world market, especially in countries with a population in a situation of social vulnerability. However, Fusarium wilt, caused by the fungus Fusarium oxysporum f.sp. cubense race 4 Subtropical (Foc ST4), emerges as a serious threat to banana production, requiring the development of resistant cultivars based on biotechnological strategies, such as the induction of mutation in tissue culture. This study aimed to identify and characterize genetic variation in somaclones resistant to Fusarium oxysporum f.sp. cubense subtropical race 4 (Foc ST4), derived from ‘Grand Naine’ bananas, by molecular markers based on retrotransposons IRAP (Inter-retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism). Nine combinations of IRAP and six combinations of REMAP primers were used. The low number of polymorphic bands did not allow for genetic diversity studies; however, ten polymorphic bands between the somaclones and control were sequenced. Of these, three presented good base calling and were aligned, namely, 1AF, 2AF, and 3AF bands. Only the 1AF band presented function related to stress response with homology to a calcium-binding protein. These proteins act early in plant infection as secondary messengers activated by pathogen-associated molecular patterns (PAMPs), initiating the cascade of plant defense signals. The fact that this band is present in all somaclones reinforces previous assessments of their resistance to Foc ST4. The use of markers IRAP and REMAP produced polymorphic bands that can, through future primer design and field validations, accelerate the identification of resistant banana genotypes for use in banana genetic breeding programs.

Quantifying Invasiveness Risk of Non‐Native Freshwater Fishes in South Korea Under a Changing Climate

ABSTRACTBiological invasions pose a serious threat to native biodiversity, and climate change intensifies the risk of non‐native species invasion in human‐altered environments, particularly in freshwater ecosystems. In South Korean freshwaters, the number of non‐native fish species increased significantly since the early 1950s. We screened risk of invasion by 43 freshwater fish species in South Korea using the Aquatic Species Invasiveness Screening Kit (AS‐ISK). Of these species, 26 (~ 61%) posed a high to very high risk of invasiveness. Black bullhead Ameiurus melas, brown bullhead Ameiurus nebulosus, North African catfish Clarias gariepinus, green sunfish Lepomis cyanellus, black carp Mylopharyngodon piceus, round goby Neogobius melanostomus, and Chinese sleeper Perccottus glenii were very high risk under current and predicted climate conditions. Striped snakehead Channa striata, longear sunfish Lepomis megalotis, smallmouth bass Micropterus dolomieu, white perch Morone americana, Eurasian perch Perca fluviatilis, and rudd Scardinius erythrophthalmus were high risk only under predicted climate conditions. Present risks were generally consistent with previous qualitative assessment, with some discrepancies, likely due to unobserved or under‐reported invasive traits of some species or a better ability of the present semi‐quantitative approach to estimate potential risks. Our results will inform South Korean decision‐making to potentially enhance regional cooperation across East Asia against transboundary introductions of aquatic invasive species.

Community Mobilisation for Human Sample Collection in Sensitive Communities: Experiences from Granular Mapping of Schistosomiasis and Soil-Transmitted Helminths in Ekiti State, South West, Nigeria

Community mobilisation is a vital process for raising awareness and increasing participation in healthcare interventions, research, and programmes that require human sample collection and mass management. In this report, we present the community mobilisation approach undertaken for the implementation of the operational mapping and assessment of granular schistosomiasis and soil-transmitted helminths in Ekiti State, Nigeria. The mobilisation was conducted in 177 communities/wards of the 16 local government areas. A total of 15,340 urine and stool samples were collected in 34 days. The efficacy and success of the strategy were evaluated through the following three performance metrics: community compliance rate, the participant response rate at the community level, and the overall compliance response rate of the four most sensitive LGAs. Community compliance was 93.7% as sample collection was denied in nine communities and two other communities demanded the return of the collected samples despite our mobilisation effort because of cultural bias and myths that connect the collection of stool and urine samples to ritual activities in the local context. The participant response rate at the community level was 86.7%. Three of the four sensitive LGAs (based on previous assessment programmes) demonstrated satisfactory compliance rates of 100%, while a response rate of 64.0% was computed for one of the LGAs. We believe our approach contributed to effective community mobilisation and awareness and that the developed model has the potential to improve participation rates in large healthcare assessments and intervention programmes.

Iron Age Fur Skin Tanning – a Sustainable Practice?

Tanning is among the most polluting industries in the world. Industrial-produced hides and skins are fully or pre-tanned with highly polluting chromium salts. The purpose of the study was to gain new knowledge about Iron Age tanning methods to clarify whether sustainable tanning methods can be developed based on this. Fur skin capes, uncovered in Jutland bogs, from Baunsø Mose (20-220 AD), Borremose I (365-116 BC), Huldremose I (1-174 AD) and Vindum Mose (386-203 BC) were analysed by Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR), Gas Chromatography-Mass Spectrometry (GC-MS) and morphological assessment of the skin fibres to identify tanning substances and material condition. Analyses were supplemented with source studies of previous visual assessment of the capes and measured shrinkage temperature of leather and skins excavated from bogs. Our results show that only the samples from Baunsø Mose, Borremose I and Huldremose I contain vegetable tannins. Furthermore, Baunsø Mose contains cow fat and Borremose I, Huldremose I and Vindum sheep fat. All contain indications of the presence of aluminum and iron compounds. The samples are decomposed to varying extents. Remnants from conservation were detected on Huldremose I, Baunsø Mose and Vindum Mose.

Xerophilic Aspergillaceae Dominate the Communities of Culturable Fungi in the Mound Nests of the Western Thatching Ant (Formica obscuripes)

The nests of mound-building ants are unexplored reservoirs of fungal diversity. A previous assessment of this diversity in the nests of Formica ulkei suggested that water availability may be a determinant of the composition of this mycota. To investigate this question, we recovered 3594 isolates of filamentous Ascomycota from the nests of Formica obscuripes and adjacent, non-nest sites, employing Dichloran Rose Bengal agar (DRBA), Dichloran Rose Bengal agar containing glycerol (DRBAG), and malt extract agar containing sucrose (MEA20S). Higher numbers of fungi were isolated from the tops of mounds than from within mounds and non-mound sites. Mound nest soils were dominated by members of the family Aspergillaceae, and up to 50% of the colonies isolated on DRBAG belonged to the genus Aspergillus. Pseudogymnoascus pannorum and species of Talaromyces were also present in higher numbers in mound soils. Species of Penicillium were more abundant in non-nest soils, where they accounted for over 66% of isolates on DRBA. All Aspergillaceae assessed for xerotolerance on a medium augmented with glycerol or sucrose were xerophilic. These results, and our observation that the nests of F. obscuripes are low-water environments, indicate that water availability influences the structure of the fungal communities in these animal-modified habitats.

Farmers’ willingness to introduce short-rotation tree plantations on agricultural land: A case study in southern Sweden

To meet climate targets, expanding Populus spp. tree cultivation is proposed as a potential biomass feedstock, especially on agricultural land that does not come into conflict with food production. However, biomass potential assessments typically overlook landowners' perspectives, risking a gap between theoretical potentials and realisation. Here, we test empirical consequences of two hypotheses based on a survey targeting southern Swedish farmers: 1) Relying exclusively on agricultural land cover data to identify abandoned agricultural land leads to an overestimation of the total agricultural land that can be utilised for future biomass production from Populus spp. feedstocks. 2) The absence of data on farmers' intentions to cultivate fast-growing tree species on agricultural land leads to overestimation of the potential biomass supply from Populus spp. in biomass assessments. Findings suggest that less than 50 % of farmers with unsubsidised arable land, which is often assumed to be abandoned, would consider cultivating these tree species on this type of land (26 % [7–48]). Furthermore, only 11 % [6–17] would consider cultivating Populus spp. on agricultural land overall during 2021–2030, indicating a generally low level of interest among farmers. However, higher rates were observed in forested areas. The projected near-future cultivation potential of 2.0 kha [1.1–3.0] suggests an at least threefold overestimation in previous theoretical assessments. This study highlights a disparity between biophysical land data and producer perspectives, showing that neglecting farmers’ perspectives risks overestimating the biomass supply, potentially leading to misguided expectations and inefficient policies. Our findings support targeted policy recommendations.

Five key issues of assessing nature’s contribution to people toward landscape sustainability

Landscape sustainability aims to consistently provide long-term, landscape-specific ecosystem services. The concept of nature’s contributions to people (NCP), broader than that of ecosystem services, has sparked methodological controversy regarding the new assessment framework of NCP. Accordingly, for further exploration of the essence of landscape sustainability, a way forward in better assessing NCP that differs from previous assessments of ecosystem service is necessary. Five key issues for assessing NCP are summarized in this paper, including: 1) how to determine generalized or landscape-specific parameters; 2) how to identify different people’s needs from a landscape; 3) how to deal with the complexity of non-material NCP at landscape scale; 4) how to evaluate the relationship between different NCP from a landscape; and 5) how to assess landscape multifunctionality based on NCP. The way to deal with these key issues is discussed, including: determining generalized or landscape-specific parameters based on research targets; tracking nature’s contributions to different people’s needs considering spatial flow; forming new spatial data for non-material NCP; evaluating the relationship between different NCP depending on threshold of benefit; and assessing landscape multifunctionality based on the relations of NCP.

Psychometric adequacy of a survey attitude scale

As attitude toward surveys contributes to nonresponse, this exploratory study assesses the psychometric adequacy and predictive validity of a survey attitude scale among a target population in the United States. Findings suggest respondents hold a positive attitude toward surveys, in terms of value and enjoyment, and an assessment of the scale’s psychometric properties confirmed acceptable reliability and validity metrics that align with previous assessments. Overall, the results suggest a survey attitude scale can serve as a useful metric and accurate predictor of survey response, which contributes to ongoing discussions of nonresponse and the contemporary survey climate among survey practitioners.

Clinical characteristics and suicidal ideation as predictors of suicide: prospective study of 1000 referrals to general adult psychiatry.

Questions often follow the suicide of someone who presented to general adult psychiatry (GAP) when expressing suicidal thoughts: 'Why were they not admitted, or managed differently, when they said they were suicidal?' Answering these questions requires knowledge of the prevalence of suicidal ideation in patients presenting to GAP. Therefore, we determined the general clinical characteristics, including suicidal ideation, of a large sample of patients presenting to a GAP emergency assessment service or referred as non-emergencies to a GAP service. Suicidal ideation was very common, being present in 76.4% of emergency presentations and 33.4% of non-emergency referrals. It was very weakly associated with suicide, varied between different diagnostic categories, and previous assessment by GAP did not appear to affect it. The suicide rate during the contingent episode of care was estimated as 66 per 100 000 episodes. This, and other evidence, shows that suicide cannot be predicted with an accuracy that is useful for clinical decision-making. This is not widely appreciated but has serious consequences for patients and healthcare resources.

Co-governance of open pit mines across China based on exposure risk assessment of particulate matter

Particulate matters (PMs) generated by open-pit mines (OPMs) pose a serious threat to human health and has attracted significant attention from government agencies. Previous exposure risk assessments of OPM-generated PMs focused only on environmental factors associated with individual mines, lacking large-scale assessments that integrate environmental factors with human behavior. Thus, to effectively implement governance policies for OPMs, we used the D8 algorithm to assess the risk of exposure to OPM-generated PM2.5 and PM10 across 315 Chinese cities and employed spatial Durbin modeling to explore how to co-govern the exposure risk from the perspectives of governments, companies, and the public. The results showed that 99.3% of the cities were affected by PMs originating from OPMs, and 56.5% of these cities experience a high exposure risk, approximately two-thirds of which are nonresource-based cities. The government's focus on digitalization, extractive companies' access to investment, and public participation in environmental management are key factors in reducing exposure. Cross-regional cooperation with regards to public participation and company investment is essential for reducing the effect of exposure risks on the surrounding area. This study can provide reference for the green mining construction and urban habitat improvement.

Grassland ecosystem service value in the Tibetan Plateau has not recovered during 1995–2015

Grasslands in the Tibetan Plateau (TP) provide substantial ecosystem services (ESs) but face considerable challenges. Previous assessments of grassland ecosystem service value (GESV) have revealed substantial uncertainties due to inconsistencies in GES classifications, land use data, and assessment methods. Here, we propose a new classification system, present merged land use data, and develop integrated comprehensive assessment methods to reassess the GESV in the TP from 1995 to 2015, and then analyzed spatiotemporal changes and driving factors. Our findings indicate that: 1) grasslands dominate the TP, covering approximately 70 % of the land surface, but have been continuously declining, especially during 1995–2000. 2) The value equivalent factor method tends to overestimate GESV, and our reassessment further emphasized the critical role of grasslands. 3) Temporally, GESV exhibited a measured recovery post-2000, though it remained below initial levels. 4) Spatially, GESV declined from southeast to northwest, mainly influenced by precipitation, although this spatial gradient has gradually diminished. 5) Both climatic and anthropogenic factors significantly influenced changes in GESV, but even dual factors had limited explanatory power. Our reassessment sheds light on the spatiotemporal changes and drivers of GESV in the TP over two decades, which is important for grassland management in the region. However, methodological limitations remain, particularly with regard to data accuracy; and further exploration of spatial heterogeneity and multi-factor interactions are also required.

The ‘glass route’ for improving the sustainability of photovoltaic technology

The ‘glass route’ for improving the sustainability of photovoltaic technology Building upon previous life cycle assessments of photovoltaic technologies, the sustainability of photovoltaic modules will be increased by improving the mechanical and optical properties of the glass and plastics (PET and EVA) used to manufacture the modules. The reduction of ion migration towards the active solar cell will extend the lifetime of the modules and facilitate its recyclability at the end of life. Bismuth oxide has been tested as a material to improve the properties of the glass cover.

Affective dynamics surrounding craving, non-heavy alcohol use and binge drinking in female patients with alcohol use disorder and controls: An experience sampling method study.

Studies show that higher levels of positive affect (PA) and lower levels of negative affect (NA) are related to craving and alcohol consumption at a daily level in men, but little is known on these associations at a momentary level, and whether they are present in women. This study measured the dynamics of within-person NA and PA surrounding craving, non-heavy alcohol use and binge drinking in women with alcohol use disorder (AUD) and female controls without AUD. 53 female patients with AUD and 75 female controls, all recruited in Belgium, were included in an experience sampling study where they reported on momentary NA, PA, craving and alcohol use in daily life over a period of 12 months. Assessments occurred eight times a day on Thursdays, Fridays and Saturdays in seven bursts of three weeks. Within-person NA at a previous assessment (t-1) predicted craving at the current assessment (t0) in patients with AUD in a positive linear [β = 0.043; 95% confidence interval (CI) = 0.002, 0.057; P = 0.041] and quadratic fashion (β = 0.034; CI = 0.011, 0.057; P = 0.004). Within-person PA at t-1 predicted craving at t0 in patients with AUD with a positive quadratic relation (β = 0.042; CI = 0.08, 0.065; P < 0.001). Within-person NA at t-1 negatively predicted non-heavy alcohol use at t0 in a linear fashion in controls (β = -0.495; CI = -0.677, -0.312; P < 0.001) and patients with AUD (β = -0.276; CI = -0.421, -0.132; P < 0.001). Within-person PA at t-1 significantly predicted non-heavy alcohol use at t0 with a positive linear term (β = 0.470; CI = 0.329, 0.610; P < 0.001) in controls, but with a positive linear term (β = 0.399; CI = 0.260, 0.454; P < 0.001) and a positive quadratic term (β = 0.203; CI = 0.060, 0.347; P = 0.003) in patients with AUD. Within-person NA at t-1 predicted binge drinking at t0 in patients with AUD with a significant quadratic term (β = 0.236; CI = 0.060, 0.412; P = 0.008), but not for controls. Within-person PA at t-1 predicted binge drinking at t0 in patients with AUD with a significant quadratic term (β = 0.378; CI = 0.215, 0.542; P < 0.001), and this was also the case for controls (β = 0.487; CI = 0.158, 0.770; P < 0.001). Non-heavy alcohol use at t0 predicted lower levels of NA at t+1 in both patients with AUD (β = -0.161; SE = 0.044; CI = -0.248, 0.074; P = 0.001) and controls (β = -0.114; CI = -0.198, -0.029; P = 0.010). Non-heavy alcohol use at t0 also predicted higher levels of PA at t+1 in both patients with AUD (β = 0.181; CI = 0.088, 0.274; P < 0.001) and controls (β = 0.189; CI = 0.101, 0.278; P < 0.001). The momentary relation between affect and craving or alcohol use seems to be non-linear in female patients with alcohol use disorder, whereby a worse mood predicts subsequent alcohol use, though more for binge drinking than for non-heavy alcohol use.