Prevention Methods Research Articles

Proteomic and lipidomic analysis of the mechanism underlying astragaloside IV in mitigating ferroptosis through hypoxia-inducible factor 1α/heme oxygenase 1 pathway in renal tubular epithelial cells in diabetic kidney disease

Ethnopharmacological relevanceThe limitations of modern medicine in mitigating the pathological process of diabetic kidney disease (DKD) necessitate novel, precise, and effective prevention and treatment methods. Huangqi, the root of Astragalus membranaceus Fisch. ex Bunge has been used in traditional Chinese medicine for various kidney ailments. Astragaloside IV (AS-IV), the primary pharmacologically active compound in A. membranaceus, is involved in lipid metabolism regulation; however, its potential in ameliorating renal damage in DKD remains unexplored. Aim of the studyTo elucidate the specific mechanism by which AS-IV moderates DKD progression. Materials and methodsA murine model of DKD and high glucose-induced HK-2 cells were treated with AS-IV. Furthermore, multiomics analysis, molecular docking, and molecular dynamics simulations were performed to elucidate the mechanism of action of AS-IV in DKD, which was validated using molecular biological methods. ResultsAS-IV regulated glucose and lipid metabolism in DKD, thereby mitigating lipid deposition in the kidneys. Proteomic analysis identified 12 proteins associated with lipid metabolism regulated by AS-IV in the DKD renal tissue. Additionally, lipid metabolomic analysis revealed that AS-IV upregulated and downregulated 4 beneficial and 79 harmful lipid metabolites, respectively. Multiomics analysis further indicated a positive correlation between the top-ranked differential protein heme oxygenase (HMOX)1 and the levels of various harmful lipid metabolites and a negative correlation with the levels of beneficial lipid metabolites. Furthermore, enrichment of both ferroptosis and hypoxia-inducible factor (HIF)-1 signaling pathways during the AS-IV treatment of DKD was observed using proteomic analysis. Validation results showed that AS-IV effectively reduced ferroptosis in DKD-affected renal tubular epithelial cells by inhibiting HIF-1α/HMOX1 pathway activity, upregulating glutathione peroxidase-4 and ferritin heavy chain-1 expression, and downregulating acyl-CoA synthetase long-chain family member-4 and transferrin receptor-1 expression. Our findings demonstrate the potential of AS-IV in mitigating DKD pathology by downregulating the HIF-1α/HMOX1 signaling pathway, thereby averting ferroptosis in renal tubular epithelial cells. ConclusionsAS-IV is a promising treatment strategy for DKD via the inhibition of ferroptosis in renal tubular epithelial cells. The findings of this study may help facilitate the development of novel therapeutic strategies.

Malaria in Pregnancy, Current Challenges, and Emerging Prevention Strategies in a Warming Climate.

Malaria still presents a grave threat to the health of pregnancies worldwide with prevention currently stalling as traditional control and prevention strategies are limited by both insecticide and drug resistance. Furthermore, climate change is bringing malaria to locations where it was once eradicated and intensifying malaria in other areas. Even where malaria is not currently common, obstetricians will need to understand the pathogenesis of the disease, how it is transmitted, methods for prevention and treatment in pregnancy, and promising emerging strategies such as vaccines. A renewed global response is needed for this age-old disease in which pregnancy poses specific susceptibility.

Study on methane degradation by microbial agents based on chelating wetting agent carriers

Due to the low permeability characteristics of the deep gas-containing coal seam, the conventional prevention and control measures that cannot solve the problems of gas outbursts are unsatisfactory for the prevention and control of the coal and gas outbursts disaster. Therefore, in this study, a strain of methane-oxidizing bacteria M07 with high-pressure resistance, strong resistance, and high methane degradation rate was selected from coal mines. The growth and degradation abilities of M07 in chelating wetting agent solutions to assess its adaptability and find the optimal agent-to-M07 ratio. It provides a new method for integrating the reduction of impact tendency and gas pressure in deep coal mines. The experimental results show that M07 is a Gram-positive bacterium of the genus Bacillus, which has strong resistance and adaptability to high-pressure water injection. By degrading 70 mol of methane, M07 produces 1 mol of carbon dioxide, which can reduce gas pressure and reduce the risk of gas outbursts in coal mines. As the experiment proves, the best effect was achieved when the M07 concentration of the chelating wetting agent was 0.05%. The methane-oxidizing bacteria based on the chelating wetting agent as carriers prove a new prevention and control method for the integrated prevention and control of coal and gas outbursts in coal mines and also provide a new idea for microbial application in coal mine disaster control.

Reliability analysis and preventive maintenance policy for consecutive k$k$‐out‐of‐n:F$n: F$ balanced system under failure criterion operating in shock environment

AbstractThis paper presents a consecutive ‐out‐of‐: balance system in a shock environment, where the state transition of component is induced by external shocks. If a predetermined threshold number of effective shocks are applied to component in a critical state, the component will fail. The state of the system is defined by the number of consecutive failing component groups in the system, which leads to system failure when a critical number of consecutive failing components is reached. To minimize maintenance costs, we propose a preventive maintenance method with an optimization model. We use finite Markov chain imbedding and Phase‐type distribution to calculate component group failure rates and associated probability functions in discrete and continuous time, respectively. The validity and accuracy of the model are confirmed by numerical examples and Monte Carlo simulations.

Contamination in Heat Exchangers: Types, Energy Effects and Prevention Methods

Exchangers are thoroughly used equipment for heat transfer. These equipment play a climacteric role in variegated business administration and buildings by providing heat exchange between two fluids. However, over time, exchangers can be subject to variegated problems such as contamination and sediment build-up. This can diminish heat transfer efficiency, leading to energy waste and equipment malfunctions. Calcification is a problem that comes off when water becomes saturated with hard minerals and exceeds the solubility of these minerals. These minerals precipitate as a consequence of water evaporation or chemical reactions and form a solid layer called limestone. Limescale can bring variegated problems in homes, business administration, and water transportation systems. Lime accumulates on heat transfer superficies, reducing the superficies area of ​​these superficies. This reduces the superficies area available for heat transfer and inhibits heat transfer. The thermal conductivity of lime is lower than water. Scale, which is the formation of a scale layer on heat transfer superficies, reduces the thermal conductivity of these superficies and prevents heat transfer. This study focuses on the types of contamination in heat exchangers, the effect of contamination on heat transfer and other factors, and methods of clogging.

Reviving health mediation during the COVID-19 crisis and beyond: an implementation study in deprived neighbourhoods of Marseille, France.

In 2020, during France's COVID-19 response, healthcare professionals from a hospital and an association initiated health mediation interventions in Marseille's vulnerable neighbourhoods, funded by the regional health authorities. This mixed method research evaluates the CORHESAN program that lasted until June 2022. We examined CORHESAN documents and reports, conducted interviews, and analysed activity data, comparing it to the COVID-19 hotspots identified on a weekly basis at the neighbourhood level, using generalised linear mixed models (GLMMs). CORHESAN was implemented by a team of up to nine health mediators, six private nurses hired on an ad hoc basis, supervised by a general coordinator and two part-time medical and nursing coordinators. Multiple partnerships were established with shelters, associations, social-housing landlords and local institutions. The team accompanied 6,253 people affected by COVID-19 or contact in the practical implementation of their isolation and contact tracing. Of the 5,180 nasopharyngeal samples for RT-PCR and 1,875 for antigenic testing: 12% were taken at home and 27% in partner facilities in the targeted neighbourhoods; 32% were taken from symptomatic patients and 30% in the context of contact tracing; and 40% were positive. Multiple awareness sessions on prevention methods and distributions of personal protection kits and self-diagnostic tests were conducted in the streets, in shelters, in associations or at home. A total of 5,929 doses of COVID-19 vaccine were administered in a walk-in vaccination centre, at temporary street vaccination posts, during operations at partner facilities, or during home-visits to patients with limited autonomy. GLMMs showed that the intervention significantly targeted its testing interventions in neighbourhoods with socioeconomic disadvantage and/or past under-testing (adjusted odds ratio (aOR), 2.75 [1.50-5.00]) and those with high hotspot level (aOR for level-3 versus level-0, 1.83 [1.24-2.71]). The pandemic emphasised the potential of health mediation interventions to address health disparities. Building on this, a new program began in July 2022, aiming at enhancing cancer screening and vaccinations in deprived areas of Marseille. Evaluations are ongoing to assess its activities and impact, and provide evidence to future implementation initiatives.

340 Investigating the Effectiveness of Folic Acid-Fortified Iodized Salt on Serum Folate Concentration- a Primary Prevention Strategy for Spina Bifida in India

Abstract Aim Investigate the effectiveness of folic acid-fortified iodized salt in increasing serum folate concentrations among non-pregnant and non-lactating women of reproductive age Method All households in four rural Indian villages agreed to consume FA-fortified iodized salt (study salt) in a pre- and post-intervention study. Pre- and post-intervention serum folate levels were analysed from 83 eligible participants. A cohort study was done after consumption of approximately 300 mcg of FA daily using double fortified salt (FA + Iodine). We assessed the differences in median serum folate concentrations at baseline and 4 months. Results The median serum folate concentration increased from 14.6 nmol/L at baseline to 54.4 nmol/L at end of study. Two-tailed Wilcoxon Signed Rank Test showed the difference to be highly significant (p-value = 1.8 × 10-14). The villagers found the salt acceptable in color, taste and cooking properties. No untoward effects related to the study salt were noted. Conclusions Folic Acid for the prevention of Spina Bifida has over 3 decades of evidence to support it. In 2023, the World Health Assembly unanimously recommended FA to prevent incidence of Spina Bifida. India, currently estimated to bear a third of the global Spina Bifida burden, poses a unique challenge to mandatory fortification due to the heterogeneity in population, culture, and dietary patterns. FA-fortified salt presents an equitable opportunity to reach the entire population of India and avert a significant burden of Spina Bifida and its associated morbidity and mortality. Moreover, salt fortification infrastructure currently exists so FA-fortified salt poses a cost-effective primary prevention method.

Intrusion Detection and Prevention Systems for Ad-Hoc Networks

Ad-hoc networks are vulnerable to various types of attacks due to their decentralized and dynamic nature. Intrusion detection and prevention systems (IDPSs) have been proposed to secure these networks by detecting and preventing attacks. This paper presents a comprehensive survey of IDPSs for ad-hoc networks. The survey covers the recent advancements in IDPSs for ad- hoc networks, including the detection techniques, prevention methods, and deployment strategies. The paper also discusses the challenges and open issues in developing effective IDPSs for ad-hoc networks. The survey concludes with a discussion on the future research directions for IDPSs in ad-hoc networks. The survey provides a valuable reference for researchers and practitioners working on securing ad-hoc networks.

Boundarics in Biomedicine.

"Boundarics in Biomedicine" is a cutting-edge interdisciplinary discipline, which is of great significance for understanding the origin of life, the interaction between internal and external environments, and the mechanism of disease occurrence and evolution. Here, the definition of Boundarics in Biomedicine is first described, including its connotation, research object, research method, challenges, and future perspectives. "Boundarics in Biomedicine" is a cutting-edge interdisciplinary discipline involving multiple fields (e.g., bioscience, medicine, chemistry, materials science, and information science) dedicated to investigating and solving key scientific questions in the formation, identification, and evolution of living organism boundaries. Specifically, it encompasses 3 levels: (a) the boundary between the living organism and the external environment, (b) internal boundary within living organism, and (c) the boundary related to disease in living organism. The advancement of research in Boundarics in Biomedicine is of great scientific significance for understanding the origin of life, the interaction between internal and external environments, and the mechanism of disease occurrence and evolution, thus providing novel principles, technologies, and methods for early diagnosis and prevention of major diseases, personalized drug development, and prognosis assessment (Fig. 1).

Effects of Luteolin in an In Vitro Model of Porcine Intestinal Infections.

Intestinal infections caused by Escherichia coli and Salmonella enterica pose a huge economic burden on the swine industry that is exacerbated by the development of antimicrobial resistance in these pathogens, thus raising the need for alternative prevention and treatment methods. Our aim was to test the beneficial effects of the flavonoid luteolin in an in vitro model of porcine intestinal infections. We infected the porcine intestinal epithelial cell line IPEC-J2 with E. coli and S. enterica subsp. enterica serovar Typhimurium (106 CFU/mL) with or without previous, concurrent, or subsequent treatment with luteolin (25 or 50 µg/mL), and measured the changes in the reactive oxygen species and interleukin-6 and -8 levels of cells. We also tested the ability of luteolin to inhibit the adhesion of bacteria to the cell layer, and to counteract the barrier integrity damage caused by the pathogens. Luteolin was able to alleviate oxidative stress, inflammation, and barrier integrity damage, but it could not inhibit the adhesion of bacteria to IPEC-J2 cells. Luteolin is a promising candidate to be used in intestinal infections of pigs, however, further studies are needed to confirm its efficacy. The use of luteolin in the future could ultimately lead to a reduced need for antibiotics in pig production.

ОПЫТ РЕАЛИЗАЦИИ ПИЛОТНОГО ПРОЕКТА «ГРОДНЕНСКАЯ УНИВЕРСИТЕТСКАЯ КЛИНИКА» В АСПЕКТЕ ЭФФЕКТИВНОСТИ НАУЧНОЙ ДЕЯТЕЛЬНОСТИ

The development and prompt implementation of advanced medical methods of diagnosis, treatment and prevention, based on the latest achievements of science and technology is a healthcare focus area for any state. The purpose of this publication is to summarize the experience of the pilot project “Grodno University Clinic” implementation regarding the efficiency of scientific activities. 12 indices of the University clinic innovative development were included in the model for final results activities of the clinic’s departments. Significant growth in innovation has been achieved, 25 employees of the clinic participate in the educational process of the medical university, 15 theses are being carried out by the clinic staff and teaching staff, and 6 patents have been jointly received. 11 initiative research projects are planned and carried out at the clinic. In addition, funded projects are being implemented, the implementation of which made it possible to attract 209,850 BYN in 2022. Scientific and practical conference “Innovative achievements in the work of Grodno University Clinic” is held annually as a part of the “Perspective plan for the development of the health care institution “Grodno University Clinic” implementation regarding the increase of the efficiency of scientific activities and personnel of the highest scientific qualifications. The clinic conducts clinical trials of medicines and medical products; income for 2022 amounted to 523.88K BYN. The experience of this project implementation makes it possible to draw the following conclusions. The presented interim results of pilot project “Grodno University Clinic” implementation regarding the efficiency of scientific activities allow us to assess positively this initiative transformation of the model for organizing the functioning of a multidisciplinary medical healthcare institution. The marked tendencies in the dynamics of the main indices reflect the efficiency of scientific research in the applied healthcare areas of high priority. The positive influence on innovation impacted by this favorable environment under a new integration project of science and practice for national healthcare is shown. Further research on the role and place of university clinics in the Republic of Belarus under their contribution and influence on the training of highly qualified scientific staff for healthcare, the development and implementation of import-substituting and export-oriented innovative methods of diagnosis, treatment and prevention in practical medicine are considered to be relevant.

The Effect of a Pelvic Clock Tool Exercise on Central Muscle Endurance, Trunk Range of Motion, and Pain Intensity in Middle-aged Women With Nonspecific Chronic Low Back Pain

Background and Aims Despite the advancement of medicine in managing spinal diseases and the increasing development of treatment methods, back pain has remained one of the significant public health problems in industrial and non-industrial countries. Therefore, the present study aimed to investigate the effect of a course of exercise with a pelvic clock device on the endurance of central body muscles, torso range of motion, and pain intensity in middle-aged women with nonspecific chronic low back pain. Methods The present study was quasi-experimental with a pre-test-post-test design and a control group. The statistical population of the present study consisted of middle-aged women with nonspecific chronic low back pain in the age range of 35-50. The statistical sample of the study included 30 middle-aged women who were purposefully and accessibly selected from the study›s statistical population based on the inclusion and exclusion criteria. The exercise protocol was performed with a pelvic clock device for 8 weeks (3 sessions of 45 minutes per week) by a researcher in a sports club. Central body muscle endurance indices were measured using the McGill test (trunk flexural endurance test for abdominal muscle endurance) and modified Bearing Sorenson test. Also, the trunk range of motion and pain intensity were assessed using Schubert's modified test and visual analog scale. The measurement was made while the control group participated only in the pre-test and post-test stages. The paired t-test and independent t-test were used for intra-group and inter-group comparisons, respectively, at a significance level of 0.05. Results The results showed that 8 weeks of exercises with a pelvic clock device could affect the endurance of the muscles of the central body, the range of motion of the trunk, and the severity of pain in middle-aged women with nonspecific chronic low back pain. However, it did not affect the control group (P≤0.05). Conclusion According to the present study findings, it is suggested that exercises with pelvic clock device are used as a cheap and practical modality to increase endurance of central body muscles and torso range of motion but reduce pain in middle-aged women with nonspecific chronic low back pain. It could also be used as a method of prevention.

Security assessment and preventive control of combined electricity and heat systems with distributed electric thermal storage

Achieving carbon peak and neutrality targets can be accelerated by reducing wind power curtailment (WPC). However, WPC is constrained due to the electric thermal coupling characteristics of combined heat and power. To tackle this challenge, we propose an optimal scheduling framework for combined electricity and heat systems (CEHS) with distributed electric thermal storage (DETS) and perform security assessment and preventive control. Firstly, a general model is proposed to schedule DETS for different types of customers. The customer flexibility regulation variable (CFRV) for each customer is developed to fill this knowledge gap, and the excess thermal storage stored in DETS is used for auxiliary thermal network regulation (ATNR). Secondly, a two-stage security assessment and preventive control method for CEHS with DETS is proposed to correct overloaded branch flow. Thirdly, an evaluation index is developed to evaluate the severity of overloaded branches. The results show that the method of considering CFRV can reduce WPC and operation costs compared to the traditional method. Adopting ATNR enhances the flexibility and economics of grid scheduling compared to non-ATNR. Moreover, assessments of overloaded branches in various cases show that the preventive control method mitigates overload risks.

Shedding Light on Breast Cancer, its Development, And Methods of Diagnosis and Prevention

It is a complex task to find out a specific reason of breast cancer which are based on multiple factors. Nevertheless, hormones, especially estrogens, are the ones that are mostly responsible for hampering unrestricted division of cancerous cells in the mammary gland. Fully knowing their role is pivotal for matching preventive and therapeutic plans. Chromosomal anomalies are abundant in this inborn module but they are next to BRCA1 and BRCA2, the errors that increase the risk. The diagnostic technologies which include HER2/neu testing and gene expression profiling are made to personalize the therapy practice with the intention to enhance the outcomes. The amalgam of environmental, hormonal, and genetic influences results in the creation of an individuality. The effectiveness of rehabilitation can be drastically boosted through the early identification of a disease process. A mammogram, the top screening tool for breast cancer built-in, can spot a cancer even before it shows any symptoms. In the matter of particles the breast cancer treatment has been changed greatly. Distressed tablets like trastuzumab and aromatase inhibitors in the event when accompanied by traditional chemotherapy, which in return produce efficacy with lesser side effects. Preventative policies encompass the realization of lifestyle changes as well as medical intervention. Maintaining a healthy weight, doing some extra movements, and limiting alcohol intake are all good examples of what you can do to reduce your chances of complications. Successful chemoprevention among high-risk subjects, via drugs like raloxifene and tamoxifene, has been founded. Immunotherapy is a novel approach of curing cancer by triggering immune response of the body.A review of its mechanism of operation, and preventive measures. The precision of staging is improved because of other supplementary information that is available in additional techniques like MRI and ultrasound. Such individuals can be candidates for preventive procedures such as oophorectomy or mastectomy.

Amniotic miracle: Investigating the unique development and applications of amniotic membrane in wound healing.

The perfect repair of damaged skin has always been a constant goal for scientists; however, the repair and reconstruction of skin is still a major problem and challenge in injury and burns medicine. Human amniotic membrane (hAM), with its good mechanical properties and anti-inflammatory, antioxidant and antimicrobial benefits, containing growth factors that promote wound healing, has evolved over the last few decades from simple skin sheets to high-tech dressings, such as being made into nanocomposites, hydrogels, powders, and electrostatically spun scaffolds. This paper aims to explore the historical development, applications, trends, and research hotspots of hAM in wound healing. We examined 2660 publications indexed in the Web of Science Core Collection (WoSCC) from January 1, 1975 to July 12, 2023. Utilizing bibliometric methods, we employed VOSviewer, CiteSpace, and R-bibliometrix to characterize general information, identify development trends, and highlight research hotspots. Subsequently, we identified a collection of high-quality English articles focusing on the roles of human amniotic epithelial stem cells (hAESCs), human amniotic mesenchymal stem cells (hAMSCs), and amniotic membrane (AM) scaffolds in regenerative medicine and tissue engineering. Bibliometric analysis identified Udice-French Research Universities as the most productive affiliation and Tseng S.C.G. as the most prolific author. Keyword analysis, historical direct quotations network, and thematic analysis helped us review the historical and major themes in this field. Our examination included the knowledge structure, global status, trends, and research hotspots regarding the application of hAM in wound healing. Our findings indicate that contemporary research emphasizes the preparation and application of products derived from hAM. Notably, both hAM and the cells isolated from it - hADSCs and hAESCs are prominent and promising areas of research in regenerative medicine and tissue engineering. This research delivers a comprehensive understanding of the knowledge frameworks, global dynamics, emerging patterns, and primary research foci in the realm of hAM applications for wound healing. The field is rapidly evolving, and our findings offer valuable insights for researchers. Future research outcomes are anticipated to be applied in clinical practice, enhancing methods for disease prevention, diagnosis, and treatment.

ВЛИЯНИЕ ДЕФИЦИТА ВИТАМИНА Д НА ФИЗИЧЕСКУЮ ФОРМУ И РАБОТОСПОСОБНОСТЬ СПОРТСМЕНОВ

Purpose: Study of the effect of vitamin D deficiency on the physical fitness and performance of athletes, as well as providing up-to-date information on the diagnosis and treatment of this hypovitaminosis. Material and methods: The data of domestic and foreign literature on the effect of vitamin D deficiency on the physical form and performance of athletes were analyzed. The search was carried out using electronic databases MEDLINE, Embase, Scopus, Web of Science, eLIBRARY for the period from 2007 to 2022. For the search, we used keywords and their combinations: “athletes”, “physical performance”, “stress fracture “, “vitamin D”. A total of 33 sources were analyzed. Results: Vitamin D plays an important role in the human body. With its deficiency in athletes, there is an increase in the risk of injury, a decrease in the functional abilities of muscles and an increase in the risk of stress fractures of bones. A prolonged and pronounced deficiency of this vitamin in athletes leads to a deterioration in general well-being, a disruption in the functioning of almost all body systems, a decrease in the level of working capacity and sports performance. The fundamental method of treatment and prevention of vitamin D deficiency in athletes is the correction of lifestyle, training schedule and the use of specialized supplements containing cholecalciferol. Discussion: The required amount of cholecalciferol intake varies from 400 to 2000 IU / day, depending on many factors, including the amount of sun exposure during the day, age, gender, severity of deficiency, ethnicity, etc. Athletes also need to consume sufficient amounts of micro- and macronutrients, especially calcium and magnesium. When taking cholecalciferol, one should take into account the possibility of intoxication, which occurs due to the consumption of excessive amounts of supplements of this vitamin. Conclusions: At the moment, due to the widespread prevalence of vitamin D deficiency in athletes and its negative impact on the health and performance of an athlete, there is a need for more active measures aimed at the timely diagnosis of this hypovitaminosis and its treatment.

Национальные клинические рекомендации по диагностике и лечению Ph-негативных миелопролиферативных новообразований (истинной полицитемии, эссенциальной тромбоцитемии, первичного миелофиброза) (редакция 2024 г.)

The development of National clinical guidelines on diagnosis and treatment of Ph-negative myeloproliferative neoplasms provides the knowledge basis for decision making in diagnosis, therapy, prevention, and rehabilitation in compliance with evidence-based medicine principles in order to ensure appropriate medical care in a particular clinical setting. The clinical guidelines underly the choice of optimal methods of diagnosis, therapy, prevention, and rehabilitation as part of a patient’s medical care, the development of criteria for the assessment of medical care quality, medical care standards, medical care procedures, setting standards on equipment and management of diagnostic and therapeutic procedures, and provision of continuous medical education and advanced training for healthcare professionals. In 2013, a working group was formed to develop and formulate clinical guidelines on the treatment of myeloproliferative neoplasms. These guidelines were first published in 2014, afterwards they were revised and republished. The dynamic development of current hematology presupposes constant updating of knowledge and implementation of new diagnosis and treatment methods in clinical practice. In this context, clinical guidelines present a dynamic document to be continuously amended, expanded, and updated in accordance with scientific findings and new requirements of specialists who are directly involved in treatment activities. The present edition is an upgraded version of clinical guidelines with updated information on classification, diagnosis, prognosis, current methods of therapy, and their efficacy and tolerability assessment. The guidelines are intended for oncologists, hematologists, healthcare executives, and medical students.

Machine learning and 4D-LFQ quantitative proteomic analysis explore the molecular mechanism of kidney stone formation

BackgroundNephrolithiasis, a common and chronic urological condition, exerts significant pressure on both the general public and society as a whole. The precise mechanisms of nephrolith formation remain inadequately comprehended. Nevertheless, the utilization of proteomics methods has not been employed to examine the development of renal calculi in order to efficiently hinder and manage the creation and reappearance of nephrolith. Nowadays, with the rapid development of proteomics techniques, more efficient and more accurate proteomics technique is utilized to uncover the mechanisms underlying diseases. The objective of this study was to investigate the possible alterations of HK-2 cells when exposed to varying amounts of calcium oxalate (CaOx). The aim was to understand the precise development of stone formation and recurrence, in order to find effective preventive and treatment methods. MethodsTo provide a complete view of the proteins involved in the development of nephrolithiasis, we utilized an innovative proteomics method called 4D-LFQ proteomic quantitative techniques. HK-2 cells were selected as our experimental subjects. Three groups (n = 3) of HK-2 cells were treated with intervention solutions containing 0 (negative control, NC), 1 mM, and 2 mM CaOx, respectively. For the proteins that showed differential expression, various analyses were conducted including examination of Gene Ontology (GO), Clusters of Orthologous Groups of proteins (KOG), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, enrichment analysis of protein domains, and hierarchical clustering analysis. The STRING database was used to identify the interaction network of the chosen proteins. Candidate proteins were validated using parallel reaction monitoring (PRM) in the end. ResultsAll three groups verified the repeatability of samples. According to the results of 4D-LFQ proteomic quantitative analysis, there were 120, 262, and 81 differentially expressed proteins (DEPs) in the 1 mM-VS-NC, 2 mM-VS-NC, and 2 mM-VS-1mM conditions, respectively. According to GO annotation, the functional enrichment analysis indicates that the differentially expressed proteins (DEPs) were notably enriched in promoting cell migration and the extracellular matrix, among other functions. Analysis of enrichment, based on the KEGG pathway, revealed significant enrichment of DEPs in complement and coagulation cascades, as well as in ECM-receptor (extracellular matrix-receptor) interaction and other related pathways. 14 DEPs of great interest were selected as candidate proteins, including FN1, TFRC, ITGA3, FBN1, HYOU1, SPP1, HSPA5, COL6A1, MANF, HIP1R, JUP, AXL, CTNNB1 and DSG2.The data from PRM demonstrated the variation trend of 14 DEPs was identical as 4D-LFQ proteomic quantitative analysis. ConclusionProteomics studies of CaOx-induced HK-2 cells using 4D-LFQ proteomic quantitative analysis and PRM may help to provide crucial potential target proteins and signaling pathways for elucidating the mechanism of nephrolithiasis and better treating nephrolithiasis.

Client experiences with "Dynamic Choice Prevention," a model for flexible patient-centred HIV prevention delivery in rural Eastern Africa.

Identifying the optimal approaches to offering HIV prevention to meet the needs of those at risk is a high priority, particularly given the expanding toolkit of biomedical HIV prevention options. An ongoing study in rural East African communities evaluated the uptake of choices in product, testing mode and location of care delivery through a structured patient-centred HIV prevention delivery model. In this qualitative study, we sought to understand clients' experiences of this "dynamic choice prevention model" (DCP) and highlight pathways of action to inform HIV prevention delivery models. In-depth semi-structured interviews were conducted from November 2021 through March 2022 with a purposively selected sample of n = 56 participants in DCP trials (across outpatient departments, antenatal clinics and community settings), and n = 21 healthcare providers (total n = 77). A seven-person multi-regional team translated and inductively coded transcript data. We used a framework analysis approach to identify emergent themes. Individuals taking up HIV pre-exposure prophylaxis (PrEP) reported feelings of relief, liberation from fears of acquiring HIV and satisfaction with being able to take action despite partners' behaviours. Couples used a range of approaches afforded by the study to persuade partners to get tested and opt for PrEP. Post-exposure prophylaxis (PEP) use was less common, although women welcomed it in the event of sexual coercion or assault. Participants discussed switching from PEP to PrEP after familiarizing themselves with usage and ascertaining ongoing risk. Participants felt respected by providers, trusted them and appreciated being able to contact them directly for telephone support. Prevention uptake was hindered by stigma, limited experience with and knowledge of prevention methods, gendered and generational power dynamics within intimate partnerships and families, and negative perceptions of methods due to the products themselves. Participants anticipated long-acting injectable PrEP could solve their challenges regarding pill size, daily pill burden and the likelihood of unwanted disclosure. Diverse preferences and barriers to uptake of prevention require a choice of HIV prevention options, locations and delivery modalities-but in addition, flexible, competent and friendly care provision is crucial to promote uptake. Helping clients feel valued, and addressing their unique needs and challenges, enables their agency to prioritize their health.

Liquefaction Potential Analysis of Irrigation Canals at Sidera Area-Sigi Regency

Abstract An earthquake measuring 7.5 on the Richter scale that occurred in Palu on 28 September 2018 resulted in liquefaction where the soil lost its bearing capacity due to increased pore water pressure. The liquefaction disaster caused great damage to the Gumbasa Irrigation channel, a large part of which is in the alluvial fan area. This study aims to analyze the potential of liquefaction in irrigation canals in the Sidera village area, Sigi Regency. Using SPT (Standard Penetration Test) data from 2 boreholes with a depth of ± 20 m, MASW data, and Earthquake Risk Map. Researchers analyzed with the Seed Simplified Procedure approach, The researchers analyzed the Simplified Procedure method proposed by Seed, which uses a stress-based approach that uses the ratio of soil shear strength (CRR) and earthquake-induced soil shear stress (CSR). The results of the analysis using Peak Ground Acceleration (PGA) of 0.43 and groundwater level variations of -2.85 m (borehole BM 53) and -12.5m (Borehole BM 49) show that liquefaction occurs at depths of 4-8 m (BM 53) and 14-17 m (BM 49). The value of the Liquefaction Potential Index (LPI) increases and indicates a high liquefaction potential below the water table with the highest value of 17.88. The analysis shows that liquefaction is closely related to the shallow water table, soil type, and low N-SPT values. The high liquefaction potential requires prevention methods as a form of treatment.