Prevention Of Oral Cancer Research Articles

Awareness of oral cancer and potentially malignant oral disorder among final year medical and dental undergraduate students of Chitwan Medical College, Nepal

Background: Oral Cancer (OCA) is a common malignancy in South East Asia and the burden of the disease is increasing. OCA is usually preceded by Potentially Malignant Oral Disorder (PMOD). The early detection of these diseases may limit the high mortality and morbidity. The objectives of this study were to assess the awareness of burden, associated risk fac­tors, early detection and prognosis of OCA and OPMD among final year medical and dental students..
 Methods: This cross sectional study was conducted among final year Medical and Dental undergraduate students of Chitwan Medical College, Nepal in April 2017 using a pre tested self-administered questionnaires. All the final year students present at their respective classes (Dental= 50 and Medical=75 students) responded to the questionnaires after giving informed consent. Data entered in SPPS 20 software was analyzed using Pearson Chi Square test and Fisher exact test.
 Results: Dental students had better awareness concerning PMOD. Yet, the dental students did not differ significantly from their medical coun­terpart in most of the responses related to OCA. Surprisingly, more Dental students had misconception that OCA is not preventable and early detec­tion is difficult. Few of both groups were confident in having sufficient knowledge in OCA prevention and detection. Only 18.6% of Medical stu­dents had examined PMOD and few students of both groups had exam­ined OCA.
 Conclusions: This study revealed the deficient aspect in the knowledge concerning OCA and OPMD among Medical and Dental students indicat­ing the need to emphasize on these aspects of medical education.

Risk Assessment of Smokeless Tobacco among Oral Precancer and Cancer Patients in Eastern Developmental Region of Nepal

Background:Oral potentially malignant disorders (OPMDs) and oral cancer (OC) are preventable oral mucosal diseases prevalent in Asian region. This epidemiological study aims to identify oral potentially malignant disorders (OPMDs) and oral cancer (OC), confirm histopathologically, and treat or refer these cases among the population of Eastern Development Region (EDR) of Nepal. It also attempts to assess the risk factors associated in order to compare dose–response measurements of oral habits in these patients.Methods:Cross-sectional epidemiological study was conducted over a period of 2 years in 16 districts of EDR. A total of 3,200 people were screened. A brief history was taken and visual screening examination was conducted in two phases as described by British Columbia Oral Cancer Prevention Program. Suspicious oral lesions were biopsied either by punch or scalpel after toluidine blue staining. Tissue specimen was transported to the institutional lab for histopathological processing. The reports were sent to the patients through the local leaders or organizations. Results:More than 40% of the study population either chewed areca nut and/or tobacco. Eighteen percent were smokers. OPMDs were prevalent among 468 study population with male-female ratio of 3:1. Tobacco pouch keratosis (50.4%) was the most prevalent OPMD, followed by OSF (29.1%). Fifty-two had squamous cell carcinoma and 8 had verrucous carcinoma. Conclusion:Chewing areca nut, tobacco, commercial areca nut/tobacco preparation and smoking being the major risk factors, there is high prevalence of oral cancer and OPMDs in the EDR of Nepal.

Oral cancer awareness among university students from Karachi, Pakistan

Background: Oral cancers (OC) are malignant lesions occurring in the oral cavity that include squamous cell carcinomas (SCC), salivary gland and odontogenic neoplasms. Even though it is the eighth most common malignancy globally but in Pakistan it is the second commonest type of cancer. Lack of awareness about ill-effects of preventable risk factors of oral cancer increases the burden of disease due to the associated high cost of treatment, permanent impairment and high mortality. Hence, awareness can be very helpful in prevention, control and early diagnosis of oral cancer. Methods: A cross-sectional study was carried out among university students from Karachi, Pakistan during April to May 2018. Three hundred students were approached to participate in the study of which 277 agreed to participate. Pretested questionnaire was distributed and collected data was analysed using IBM SPSS version 23. Results: There were 125 (45%) males and 152 (55%) females in the study and response rate was 94%. Sixty one percent (154/250) respondents correctly identified smoking, and tobacco chewing as possible causes of oral cancer. Almost one third (74%; 184/250) respondents correctly responded that oral cancer does not spread from person to person through touch or speaking. Sixty six percent (164/250) respondents believed that oral cancer is curable. Mean score of knowledge was higher in females (61%) than males (53%). Significantly higher number of females compared to male participants answered correctly to questions regarding cause of oral cancer, spread of disease and occurrence of oral cancer in AIDS patients. Conclusions: Participants showed poor knowledge about oral cancer. Female participants showed better knowledge compared to male counterparts. Details about oral cancer should be incorporated in the university curriculum and periodic awareness programs should be organized for students.

Hybrid Framework using Data Mining Techniques for Early Detection and Prevention of Oral Cancer

This paper presents the usage of classification and association data mining techniques for early detection and prevention of oral cancer. The indigenous dataset of 1,025 patients who visited a tertiary care centre during 2004 to 2009 was used for the research. Ten classification data mining models are designed using varied types of data mining techniques like regression analysis, classification trees and neural networks. Regression analysis models are linear regression model and logistic regression model; classification tree models are decision tree model, decision tree forest model and TreeBoost model and artificial neural networks are multilayer perceptron model, radial basis function model, group method of data handling model, cascade correlation model and probabilistic - general regression neural network model. Association rules are generated using apriori algorithm. The classification models and association rules are evaluated using various estimation parameters. Finally, a hybrid oral cancer management system is designed using the classification model and the association rules.

Advances in oral cancer detection.

High incidence of oral carcinoma and its late-stage presentation are the major global healthcare issues. The World Health Organization (WHO) has set early diagnosis and prevention of oral cancer as their primary objective. It is important to consider the time of oral screening, as it plays a pivotal role in understanding the disease prognosis. Critical signs and symptoms that can be identified during initial oral screening can improve the chances of patient's survival. Reports suggest that socio-economic factors, lack of public awareness and delays from primary health care centers are few of the major parameters that contribute to patient's mortality and morbidity. Conventional technique of visual examination of the oral lesion can effectively monitor patient mortality when exposed to risk factors. However, several disadvantages limit the clinical utility of this technique. Thus, screening aids that efficiently differentiate between a benign and malignant lesion as well as deliver information about early OSCC can ameliorate the complications associated with oral cancer diagnosis. Recent advances in optical imaging systems, such as tissue-fluorescence imaging and optical coherence tomography have been proved to be considerably efficient. Additionally, extensive research has been directed towards nanoparticle-based immunosensors, DNA analysis, and salivary proteomics. However, lack of proper clinical trials and correlation with biopsy result hinder the usage of these screening techniques in clinics. In this review, we highlight the importance of early diagnosis of oral cancer as well as discuss about the effectiveness and limitations of the recent diagnostic aids. It can be stated that public awareness regarding routine oral examination and employing screening methods that are non-invasive, robust, and economic, would enhance early stage diagnosis of oral cancer and have a positive impact on patient's survival.

Digital possibilities in the prevention and early detection of oral cancer in the WHO South-East Asia Region.

Cancers of the lip and oral cavity are the most common cancers among men in the World Health Organization (WHO) South-East Asia Region. Most cancers of the oral cavity are attributable to tobacco smoking, smokeless tobacco use and areca-nut product use, alone or in combination, and excessive consumption of alcohol. These risk factors are highly prevalent in parts of the region. This paper outlines an integrated framework for oral cancer prevention, which includes a strengthened primary health-care workforce, enhanced community engagement and a positive policy environment. Operationalizing this framework could be greatly facilitated by the application of digital technologies. Robust evidence exists for the effectiveness of using appropriately trained primary health-care workers to screen for oral cancer by oral visual examination; this can be combined with counselling for riskbehaviour modification as part of an overall strategy on noncommunicable diseases. This needs to be supported by greater overall community engagement, for example to tackle low levels of awareness of the harmful effects of smokeless tobacco and areca-nut products. A strong policy environment that supports and promotes these efforts is essential, along with the enforcement of the measures required by the WHO Framework Convention on Tobacco Control. Despite the burden of disease, oral cancer has been a neglected area of public health. This paper considers how the positively disruptive effects of digital technology may enable much-needed acceleration in prevention and control efforts.

Preliminary results of screening for pathological lesions in oral mucosa and incidence of oral cancer risk factors in adult population

Oral cancer is a significant problem among global population. In Poland a few thousands of new incidents are reported annually. Prophylaxis and early detection help decreasing the incidence rate. Screening programmes are a tool that enable to achieve that goal. The aim of this study was to evaluate prevalence of selected risk factors and pathological lesions of oral mucosa in adult population. The study group consisted of 253 patients. After undertaking a medical history in a form of a questionnaire, a conventional intraoral soft tissue examination was performed. This was followed by use of cool white light (Microlux DL Addent). Among patients following risk factors were frequently found: tobacco smoking - 20.9% patients, alcohol consumption - 2/3 of patients drink occasionally, 8.7% drink on regular basis. Over 80% patients were sexually active. 3 patients suffered from oral cancer, 21 patients gave a history of cancer in other locations, 54.2% reported a family history of cancer. Screened patients declare quite healthy diet, although level of grilled and fried meat consumption is fairly high. 37.8% patients were alarmed by the condition of the oral mucosa but only half of them reported it to their dentist. Most of the lesions were found on the buccal mucosa (88 patients, 34.8%), retromandibular triangle and palate and most of lesions were white lesions, categorized as traumatic. This preliminary study showed, that the incidence of oral cancer risk factors is high in this population. The incidence of pathological lesions of oral mucosa was also high. The study showed low awareness of oral cancer prevention, risk factors and symptoms.

Knowledge of Oral Cancer among the Fourth and Fifth Year Dental Students.

BackgroundThe literature data show insufficient knowledge and awareness about oral cancer among dental and medical students.ObjectiveTo assess the knowledge of graduating dental students regarding oral cancer and to assess their awareness regarding the prevention and early detection of oral cancer. Also student’s smoking status was examined aimed to investigate their attitude towards smoking as a risk factor for development of oral cancer and to assess the prevalence of smoking among dental students.Subjects and MethodsThe study group consisted of 83 students of the fourth and fifth years of dental medicine study who answered the questions written in the original 16-item questionnaire.ResultsMore than half of the students considered their knowledge to be good, a quarter of them regarded their knowledge as being poor. Almost 16% of students considered their knowledge to be very good, whereas only two students rated their knowledge as ‘excellent’. Students of the fifth year were more likely to be smokers compared to fourth year students (39.5% vs. 22.5%). Higher percentage of non-smokers than smokers had marked 'stop smoking' as an important preventive measure (non-smokers 91.2%, smokers 84.3%, p = 0.01).ConclusionMajority of the students of fourth and fifth year of study rate their knowledge about oral cancer as good. A large number of students of both years considered hairy leukoplakia a potentially malignant disorder. Students non smokers recognize “stop smoking” as an important preventive measure unlike students smokers who show a more realistic attitude towards smoking as a risk factor for oral cancer.

Oral health of older people: tracking soft tissue injuries for the prevention of oral cancer.

To detect oral soft tissue injuries in older people. A quantitative analytical cross-sectional study conducted in Fortaleza, Ceará. Individual interviews addressed age, gender, marital status, income, and education. The community oral health indicator was used to detect oral soft tissue injuries and their location. Most of the 821 participants aged 60-100 years were women (580; 70.6%), attended school for up to 5 years (401; 48.8%), were illiterate (201; 24.5%), were retired (608; 74.1%), and received up to 2 wages (701; 85.4%). A total of 604 participants (73.6%) wore dentures. Injuries included red patches (152; 55.9%), blisters (58; 21.3%), lesions and/or wounds (39; 14.3%), and white patches (30; 11%). Locations of injuries were the roof of the mouth (167; 61.4%), gums (62; 22.8%), cheeks (39; 14.3%), tongue (15; 5.5%), lips (15; 5.5%), and the floor of the mouth (12; 4.4%). Injuries were associated with age (p<0.001), retirement (p=0.005), education (p=0.010), dentures (p<0.001) and red patches (p<0.001). Tracking soft tissue injuries and referring older adults with suspected malignant lesions to the health team should be included as oral cancer identification and prevention measures. Furthermore, health care providers should raise older adults' awareness of the importance of regular preventive examinations.

Global Scenario of Research in Oral Cancer.

With 300,000 new cases diagnosed worldwide, oral cancer continues to be a major global public threat. In Indian subcontinent, oral cancer accounts for 30-40% of all cancer types. Early detection of oral cancer is still considered as the most effective way to improve survival. In this regard, early detection of oral premalignant lesions and conditions is quite important as it may help in prevention of oral cancer. Scientific evidence is available which clearly indicates that transition from normal epithelium to premalignancy to oral carcinoma is the result of accumulation of genetic and epigenetic alterations in a multi-step process. Proper oral screening and understanding of the genetic and environmental factors involved in oral carcinogenesis will allow the emphasis of cancer medicine to shift from the therapy of established oral cancer to the prevention of oral carcinogenesis.

Exploratory Research Focusing on Oral Cancer Prevention: Challenges of Dealing With Informational and Cognitive Barriers.

Oral cancer is a considerable public health problem, and a low level of awareness and knowledge about this tumor and its risk factors is prevalent. To gain a profound understanding of risks groups and to identify suitable communication strategies for a prevention campaign in Northern Germany, an exploratory research was realized. In semistructured face-to-face interviews, the participants of the study (n = 28) described their individual oral cancer-related perceptions and information-seeking behaviors. A computer-assisted qualitative data analysis showed a vague but also deterring picture of cancer combined with restricted attitudes toward the topic and an inactive or even avoiding information behavior. Four underlying cognitive patterns of self-distancing were identified: (a) optimistic bias, (b) fatalism, (c) hedonism, and (d) pragmatism. The main challenge of oral cancer prevention is to deal with the target groups' informational and cognitive barriers.

Prevalence of Gul Use, Its Predictors and Association With Oral Potentially Malignant Disorders and Oral Cancer Development in the Users of Noida, India: A Cross-Sectional Study

Background: Oral cancer is the third most common cancer in India which is attributed to the high tobacco burden especially due to a myriad of smokeless tobacco (SLT) products available in the market. Gul/Gul manjan is a commercially-produced, tobacco-based dentifrice, indigenous to rural India and Bangladesh, and mainly used by women. It is both highly addictive and carcinogenic; yet its prevalence, reasons for use and association with oral potentially malignant disorders (OPMDs) and oral cancer are scarcely documented in literature, making this study the first of its kind. Aim: To determine the prevalence of Gul use, its predictors and resultant OPMDs and oral cancer in such consumers. Methods: A cross-sectional study was performed among 3452 individuals visiting the Institute's Oral Health Promotion Clinic between December 2016-March 2018. Their basic demographic details, tobacco use history and abnormalities found on oral examination, were documented in the clinical record form. Data pertaining to Gul users was subjected to further statistical analysis. Results: Of the total individuals screened, 2.9% (n=101, P &lt; 0.001) consumed Gul (89.1% current and 10.9% former users), with majority female consumers (86.1%) from peri-urban Noida. It was used (up to 5 times daily) mainly to alleviate dental pain due to caries/endodontic lesions, which were present in 35.6% users ( P = 0.004). A statistically significant positive association was seen between Gul use and development of OPMDs ( P &lt; 0.001) and oral cancer ( P = 0.001), with 7.9% (n=10; 70% Gul alone users) of the overall leukoplakias/preleukoplakias (n=127), and 7.1% (n=1, Gul alone user) of all the oral cancer cases (n=14), found in Gul users. The duration of Gul use ranged up to 25 years, indicating its high dependency. Conclusion: Gul use is relatively uncommon than other SLT products in India, even among males (the major group of tobacco consumers). Apart from teeth cleaning, Gul is a cheap, easily accessible antidote for acute dental pain relief (owing to nicotine-induced analgesia) among rural women who apply it multiple times daily onto the affected teeth and adjacent gum/mucosa, often leading to its dependency. Courtesy its significant carcinogenic potential which has been clearly reported here (OPMDs and oral cancer development among users), combating/preventing its production, easy availability (in spite of the complete ban on tobacco use in dentifrices) and consumption, is essential. Therefore, the public health challenge of prevention of the use of this form of SLT ie tobacco-based dentifrices, warrants greater interest and action than that has been received thus far, which in turn would contribute to the overall mission of oral cancer prevention.

Dental Students’ Awareness About Prevention, Early Diagnosis and Referral of Patients With Oral Cancers in Tabriz in 2018

Background: Oral cancers are the 11th leading cancers worldwide, with an annual mortality rate of 500 across the world. Therefore, the aim of the present study was to evaluate the awareness of dental students about prevention, early diagnosis and referral of patients with oral cancers in Tabriz Azad University in 2018. Methods: The present descriptive, cross-sectional study was carried out in 2018 in dental students from the third to the sixth year of their studies in Tabriz Azad University using their self-reports. To this end, 153 dental students were randomly selected based on their education levels and included in the study. Data were collected using a standard questionnaire and analyzed by chi-squared test. Statistical significance was considered to be P<0.05. Results: A total of 80.4% of the students examined the oral mucosa during routine clinical examinations and 83.7% reported that they would warn their patients about the risk factors for cancers after graduation. Only 51% of the students had ever had a chance to examine patients with oral lesions. A total of 67.1% of the subjects reported that they did not have adequate awareness of prevention of oral cancers and 91.5% were interested in gaining more knowledge about oral cancers. When the subjects were questioned about knowledge about the clinical manifestations of oral cancers, 31.4% exhibited very low level of knowledge. The majority of students (78.5%) believed that an ulcer with irregular margins that lasted for more than two weeks was due to cancer. Conclusions: The results showed a moderate level of knowledge in dental students about the clinical manifestations of oral cancers, the related risk factors and how to refer patients with such cancers. The majority of the students reported inadequate information and knowledge about prevention of oral cancers and needed to gain more knowledge.

Polymethoxyflavones prevent benzo[a]pyrene/dextran sodium sulfate-induced colorectal carcinogenesis through modulating xenobiotic metabolism in ICR mice

Toona sinensis leaf extract (TSL) has been shown to have anti-tumor effects on cancer cell lines. This study aimed to investigate the chemopreventive potential and the underlying mechanism of TSL during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis.One hundred hamsters were divided into control (n = 30), carcinogenic (n = 20), preventive (n = 42), and therapeutic (n = 8) groups. The animals in carcinogenic and preventive groups were administered reverse osmosis water (carcinogenic group) or TSL (1 g/kg bw) (preventive group) by gavage daily for 4 weeks, and their bilateral pouches were painted with a 0.5% DMBA solution for 4, 9, and 12 weeks. The animals in the therapeutic group were treated with DMBA for 12 weeks prior to TSL administration for 4 weeks. Expression levels of survivin, X chromosome-linked inhibitor of apoptosis (XIAP), proliferating cell nuclear antigen (PCNA), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) proteins were analyzed by immunohistochemistry. Apoptotic activity was examined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method, cytochrome C, and poly (ADP-ribose) polymerase (PARP).In the preventive group, the results showed significant decreases not only in the incidences of squamous cell carcinoma (SCC) (50%) and epithelial dysplasia (62.5%) but also in the tumor number, tumor volume, tumor burden, and the severity of dysplastic lesions. The down-regulation of survivin, XIAP, PCNA, iNOS, and COX-2 proteins and the increased apoptotic activity indicated anti-proliferative and apoptosis-inducing abilities of TSL on DMBA-induced HBP carcinogenesis.The results suggested that TSL might be a promising candidate for the prevention of oral cancer.

Mouth Cancer Control Program in the State of Pernambuco, Brazil

Oral cancer persists as a public health problem. Thus, mechanisms that ensure active involvement of the population must be created. Within this context, the Mouth Cancer Control Program of the University of Pernambuco (UPE) has been updating and training professionals in the prevention, and differential and early diagnosis of oral cancer since 1998 by performing educational, preventive, and diagnostic activities without interruptions. CAAE: 0201.0.097.000-08. From January 2002 to January 2017, 1400 students from UPE and 300 students from other educational institutions participated in the development of the activities. A total of 8600 professionals were trained in 63 cities in Pernambuco and 59,600 individuals participated in the educational/preventive activities designed to improve self-care, prevention, and the early detection of oral cancer. A total of 2984 individuals were examined during the training. Among the oral lesions identified and investigated by biopsy, 33% were potentially malignant lesions and 12% were malignant lesions (cancer). Health education is an excellent tool for the popularization and diffusion of technical–scientific knowledge, demystifying the disease and bringing its description closer to the population, thus permitting the identification of risk factors and the search for prevention and early detection. Brazil still faces some challenges, but education and guaranteed access to and greater effectiveness of health services will provide better conditions for the prevention, early detection, and reduction of disabilities and death since oral cancer can be prevented and cured when diagnosed at an early stage.

Comparison of Oral Lesion Diagnosis with and Without Fluorescence Visualization in Population Screening

Objective: To compare oral lesion diagnosis with and without fluorescence visualization use during oral cancer prevention campaigns.

Prevention and early diagnosis of oral cancer – a literature review

INTRODUCTION: The present study identified and described scientific production on primary and secondary prevention of oral cancer worldwide. This is an integrative review of the literature, focusing on the description of oral cancer prevention experiences.METHODS: The search was performed in the Virtual Health Library (VHL), using the descriptors: early diagnosis, oral neoplasms, primary prevention and secondary prevention. We found 225 articles and selected 22 after evaluation, divided into three themes.RESULTS: Four articles awareness-raising and population-tracking campaigns with the valorization of the role of the dental surgeon and the involvement of health teams. The non-invasive diagnostic methods, considered in fifteen articles, addressed the implementation of new strategies for the early detection of this pathology by different diagnostic methods. The reorganization of the health system (3 articles) encompassed the permanent education of oral health professionals, epidemiological surveillance and reorganization of health services with integration of health care levels. The great majority of the reviewed experiences showed positive results in the prevention of oral cancer. An exception was the active search for suspected cases in the population, since the cost benefit did not compensate the expenditure with human resources for this purpose. The non-invasive methods reported are useful for screening but do not substitute the biopsia for diagnosis.

Qualitative study for betel quid cessation among oral cancer patients.

The psychoactive effects of using areca nut and its potential for dependence have been observed. However, the factors that create barriers to or promote chewing cessation are not well understood. This study aims to explore the behavioral changes of betel quid chewers who have been diagnosed with oral cancer within a transtheoretical model framework. Thirty oral cancer patients with betel quid chewing history were chosen for in-depth interviews. Qualitative content analysis was used to analyze the data and identify themes that described the behavioral changes of betel quid cessation. Our research showed that betel quid chewers with oral cancer typically experience four significant stages of behavior: pre-contemplation, contemplation, action, and maintenance. Each stage change was marked by specific characteristics. At first, chewers showed positive attitudes toward the psychoactive or social effects of betel quid. They then realized the negative effects of betel quid, such as dental or other physical problems. Some also realized that they were addicted to betel quid. When they decided to quit, most chewers reported going “cold turkey.” Some chewers successfully quit betel quid and attributed it to willpower. Those quitting because of the loss of oral functions were unable to chew anymore, though some chewers had experienced a relapse. In the maintenance stage, ex-chewers reported overcoming their addiction; however, relapse was possible. In this study, those who quit betel quid because of oral cancer usually quit tobacco and alcohol as well, with a lesser chance of recurrence. As the maintenance of chewing betel quid is multifactorial, this study provides information for betel quid cessation and oral cancer prevention.

Effectiveness of Health Belief Model on Oral Cancer Prevention in Smoker Men.

The purpose of the present study is investigating the effect of educational intervention based on health belief model (HBM) on oral cancer prevention in smoker men. This is a quasi-experimental study carried out on 200 smoker men with the age of 40 or older (100 subjects for the experimental group and 100 subjects for control group) resident in Fasa City, Fars Province, Iran, in 2017-2018. The educational intervention for the experimental group included seven educational sessions for 50 or 55min-based HBM. A questionnaire consisted of items about demographic information, knowledge, HBM constructs (perceived susceptibility, severity, benefits, barriers, self-efficacy, and cues to action) was used to measure the oral cancer prevention before and 6months after the intervention. The mean age of the men was 51.35±8.41years in the experimental group and 52.28±8.09years in the control group. Based on the obtained results, significant enhancement is observed in average scores of knowledge, perceived susceptibility, severity, benefits, self-efficacy, cues to action, and oral cancer prevention behaviors in experimental group; however, no significant changes are observed in average scores of knowledge, perceived susceptibility, severity, benefits, self-efficacy, cues to action, and oral cancer prevention behaviors of control group. Also, results indicated that, the educational program based on HBM model have positive effect on oral cancer prevention with the improvement of subject's knowledge, perceived susceptibility, severity, benefits, and self-efficacy.

Aryl hydrocarbon receptor, a tumor grade‑associated marker of oral cancer, is directly downregulated by polydatin: A pilot study.

Oral squamous cell carcinoma (OSCC) is one of the most aggressive and deadliest tumors worldwide. The aryl hydrocarbon receptor (AHR) is a nuclear transcription factor known as a dioxin receptor and mediates the toxic effects of industrial contaminants. In addition, AHR has been implicated in multiple cellular processes and its expression has been shown to play a critical role in tumorigenesis, including human oral cancer cell lines. In the present study, we evaluated the expression of AHR/HSP-90 in 25formalin‑fixed, paraffin-embedded human oral cancer specimens by IHC analysis. CYP1A1 expression was regarded as an AHR reporter gene. The data indicated a complete correlation between AHR expression and cancer grade enabling us to propose AHR as a prognostic marker of oral cancer. Moreover, in OSCC cell line CAL27, we observed the modulatory effect of polydatin, a widespread natural substance and direct precursor of resveratrol, on AHR expression. A computational approach was performed to predict the site of interaction of polydatin on the AHR surface. Our studies confirm the involvement of AHR signaling in the clinicopathological specimens of oral cancer and suggest the use of polydatin for oral cancer prevention.