Prevention Of Damage Research Articles

Nonintrusive thermal contact conductance estimation in double-layered pipelines: A reciprocity functional method perspective

Thermal contact conductance (TCC) is a critical factor in various engineering applications involving heat transfer between solid surfaces. Accurate knowledge of TCC enables a more precise thermal system analysis, leading to improved efficiency, reduced energy consumption, and prevention of thermal damages. Moreover, TCC estimation can help to identify material discontinuities or failures. A recent tool for TCC estimation is the Reciprocity Functional Method (RFM) coupled with the Classical Integral Transform Technique (CITT). This method allows the solution of inverse boundary value problems without the need of iterative methods or intrusive measurements, making it valuable for nondestructive testing of interfacial flaws between solid materials. Furthermore, it enables the computation of TCCs using analytical expressions, resulting in a computationally fast procedure. The technique involves the solution of two auxiliary problems: one for the temperature discontinuity estimation, and the other for the interfacial heat flux estimation, both at the inaccessible interface between the two contacting materials. In this study, we applied the RFM and the CITT to estimate different types of TCCs, using a three-dimensional double-layer hollow cylinder geometry as a representative model, like double-layer pipelines used in the oil industry. Temperature measurements for solving the inverse problem were considered available on the outer surface of the pipe which, in the case of a real experiment, could be obtained via an infrared camera. The results demonstrated a good estimate for various analyzed test-cases, even when the measurements were contaminated with Gaussian noises.

Study of Hydrofoil Unstable Partial Cavitation Emphasizing Unstable Sheet Cavitation Phenomenon

Abstract Unstable partial cavitation phenomenon often occurs during the operation of axial flow pumps. In this paper, the hydrofoil of an axial flow pump is taken as the research object, and the cavitation morphology at the design angle of attack is obtained by high-speed photography. And the flow characteristics under the transformation cavitation number of sheet cloud cavitation are studied by SBES turbulence model. The hydrofoil has hydrodynamic phenomena such as unstable sheet cavitation and multistage cloud cavitation. The high pressure propagating upstream from the trailing edge may be the cause of secondary cloud shedding, and the closer to the hydrofoil wall surface, the greater the intensity of this pressure. The frequency of unstable sheet cavitation shedding is 166.6 Hz. The instable closure of cavitation and the hydrofoil wall may be the main cause of unstable sheet cavitation formation. The generated quasi-periodic re-entrant jet will have a greater impact on the flow in the rear of the hydrofoil. This study provides reference for the design and operation of axial flow pumps and prevention of cavitation damage.

The NYMERIA Study: A Real-World, Multicentre Contemporary Assessment of Disease- and Patient-Related Burden and Treatment Strategies in Patients with Systemic Lupus Erythematosus.

Systemic lupus erythematosus (SLE) has a spectrum of phenotypes. Its management should target remission or low disease activity, prevention of relapses and organ damage, minimisation of drug-related harms, and optimisation of health-related quality of life. Advances in our understanding of the disease pathophysiology have expanded the treatment armamentarium with targeted biologics that demonstrate superiority over conventional drugs in controlling activity, reducing flares and glucocorticoid exposure, and improving patient-related outcomes. In view of this, there is a critical need for real-world evidence providing insight into the spectrum of activity, the treatment landscape, and unmet needs among SLE patients. Such information can support regulatory and reimbursement decision-making. The primary objective of the NYMERIA multicentre study is to generate real-world evidence on the activity state of SLE patients treated in routine care settings in Greece. The overarching aim is to capture the disease burden based on both clinical aspects and the patient perspective.

Nanomaterials Enhance Pyroptosis-Based Tumor Immunotherapy.

Pyroptosis, a pro-inflammatory and lytic programmed cell death pathway, possesses great potential for antitumor immunotherapy. By releasing cellular contents and a large number of pro-inflammatory factors, tumor cell pyroptosis can promote dendritic cell maturation, increase the intratumoral infiltration of cytotoxic T cells and natural killer cells, and reduce the number of immunosuppressive cells within the tumor. However, the efficient induction of pyroptosis and prevention of damage to normal tissues or cells is an urgent concern to be addressed. Recently, a wide variety of nanoplatforms have been designed to precisely trigger pyroptosis and activate the antitumor immune responses. This review provides an update on the progress in nanotechnology for enhancing pyroptosis-based tumor immunotherapy. Nanomaterials have shown great advantages in triggering pyroptosis by delivering pyroptosis initiators to tumors, increasing oxidative stress in tumor cells, and inducing intracellular osmotic pressure changes or ion imbalances. In addition, the challenges and future perspectives in this field are proposed to advance the clinical translation of pyroptosis-inducing nanomedicines.

Principal causes of water damage in mining roofs under giant thick topsoil–lilou coal mine

The roof water inrush disaster induced by coal mining is becoming a vital bottleneck restricting mine safety. To accurately predict the water inrush position of the coal seam roof sandstone aquifer and accurately prevent and control it, this paper takes Lilou Coal Mine in Juye Coalfield as an example. Based on the comprehensive analysis of hydrogeological data, drilling data, and geophysiological data, this paper examines the water richness of the roof aquifer, the water insulation and geological structure, and the fracture development characteristics of the roof aquifer. Starting from the general side, nine factors, including fault strength index, tectonic intersection point, tip extinguishing point, development height of water conduction crack zone, roof sandstone aquifer thickness, aquifer drilling unit inrush, geophysical prospecting water-rich anomaly area, roof key layer thickness, roof aquifer thickness, brittle plastic rock thickness ratio, etc., form the roof plate roof. The main control factors for water inrush are deeply discussed. Through the spatial analysis function of GIS, unique drawings of different evaluation indicators are drawn, and the data are normalized. Combined with the AHP hierarchical analysis method, the corresponding weight is determined. Finally, a comprehensive water inrush risk assessment map of the roof of coal seam 3 in Lilou Coal Mine is obtained. Through the verification of the coal mine water inrush survey ledger over the years, it has been found that the evaluation results of the coal seam roof water inrush model are consistent with the actual situation. The evaluation results are reasonable and accurate, which can provide a reference basis for coal seam mining and water damage prevention and control in the future.

The efficacy of selected local pesticides in prevention of leaf damage and improvement of yield in <i>Brassica rapa subsp. pekinensis L.</i> (Chinese cabbage)

The efficacy of selected local pesticides in prevention of leaf damage and improvement of yield in <i>Brassica rapa subsp. pekinensis L.</i> (Chinese cabbage)

Study on the Characteristics and Evolution Laws of Seepage Damage in Red Mud Tailings Dams

Seepage damage is a significant factor leading to red mud tailings dam failures. Laboratory tests on seepage damage were conducted to investigate the damage characteristics and distribution laws of red mud tailings dams, including soil pressure, infiltration line, pore water pressure, dam displacement, and crack evolution. The findings revealed the seepage damage mechanisms of red mud slopes, offering insights for the safe operation and seepage damage prevention of red mud tailings dams. The results showed that the higher the water level is in the red mud tailings dam, the higher position the infiltration line is when it reaches the slope face. At the highest infiltration line point of the slope surface, the increase of pore water pressure is the highest and the change of horizontal soil pressure is the highest. Consequently, increased pore water pressure leads to decreased effective stress and shear strength, increasing the susceptibility to damage. Cracks resulting from seepage damage predominantly form below the infiltration line; the higher the infiltration lines is on the slope surface, the higher the position of the main crack formations is. The displacement of the dam body primarily occurs due to the continuous expansion of major cracks; the higher the infiltration lines are on the slope surface, the larger the displacement of the dam body is.

Investigating the effects of rare ginsenosides on hyperuricemia and associated sperm damage via nontargeted metabolomics and gut microbiota

Ethnopharmacological relevanceIn ancient times, ginseng was used for hyperuricemia treatment as described in the classic traditional Chinese medical text Shang Han Lun. Recent studies have shown that common ginsenosides and rare ginsenosides (RGS) are the main active compounds in ginseng. RGS have higher activity and are less studied in the treatment of hyperuricemia. Aim of the studyTo determine whether RGS prevents and ameliorates potassium oxonate(PO)-induced hyperuricemia and concomitant spermatozoa damage in mice and the possible underlying mechanisms. Materials and methodsPotassium oxonate (PO, 300 mg/kg) induced hyperuricemia in mice via the oral administration of RGS (50, 100, or 200 mg/kg) or allopurinol (ALL, 5 mg/kg) for 35 days. Uric acid (UA) and xanthine oxidase (XO) levels were measured to assess the degree of histopathological damage in the liver, kidney, and testis, and renal creatinine (CRE), urea nitrogen (BUN), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and inflammatory factor (IL-1β) levels were measured to calculate the sperm density. Mechanisms were also explored based on blood and urine metabolomics and the gut microbiota. ResultsIn this study, we demonstrated that RGS containing Rg3, Rk1, Rg6, and Rg5 could reduce serum UA levels, inhibit serum and hepatic XO activity, reduce renal CRE and BUN levels, further restore renal SOD and GSH activities, reduce the accumulation of MDA in the kidneys, and attenuate the production of renal IL-1β. RGS was able to restore sperm density. Metabolomic analysis revealed that RGS improved sphingolipid metabolism, pyrimidine metabolism, and other metabolic pathways. 16S rDNA sequencing revealed that RGS could increase gut microbial diversity, restore the Firmicutes/Bacteroidetes (F/B) ratio, and adjust the intestinal microbial balance. Spearman's correlation analysis revealed a correlation between differentially metabolites and the gut microbiota. Lactobacillus and Akkermansia are the core genera. ConclusionRGS can be a candidate for the prevention and amelioration of hyperuricemia and concomitant sperm damage. Its mechanism of action is closely related to sphingolipid metabolism, pyrimidine metabolism, and the modulation of gut microbiota, such as Lactobacillus and Akkermansia.

Lipid-induced lipotoxic damage in liver and hepatocytes of black seabream Acanthopagrus schlegelii is mediated by endoplasmic reticulum stress

Liver is considered the critical tissue for metabolic processing of dietary nutrients including lipids. High-fat diets (HFD) can induce hepatic lipotoxic injury, but the mechanism is unclear. This study aimed to elucidate the underlying role of endoplasmic reticulum stress (ERS) in mediating HFD-induced hepatic lipotoxic injury in black seabream (Acanthopagrus schlegelii) via in vitro and in vivo experiments. For the in vitro experiment, isolated hepatocytes from black seabream were incubated for 24 h in medium (Control), or medium supplemented with 200 μM oleic acid (OA) alone or in combination with 20 μM 4-phenyl butyric acid (OA + 4-PBA). In the in vivo experiment, black seabream juveniles (3.30 ± 0.10 g) were fed diets containing lipid at 12.4% (Control) or 18.6% (HFD) for 8 weeks, after which the HFD-fed fish were divided into three groups and, on alternate days over a period of 8 days, subjected to intraperitoneal injections of 50 mg/kg 4-PBA in hydroxypropyl-β-cyclodextrin (HBC) (treatment 4-PBA) or carrier alone (treatment HFD + HBC) versus no injection (HFD). The contents of triglyceride and non-esterified fatty acid, as well as expression levels of genes and proteins involved in lipid metabolism, ERS, apoptosis and inflammation were determined in liver and isolated hepatocytes, and oil-red O staining, ultrastructural observation, flow cytometry assay of apoptosis rate, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) staining were performed on hepatocytes. The in vitro experiment showed that 4-PBA treatment significantly decreased lipid accumulation, and the OA-induced expression levels of genes/proteins linked to ERS, lipogenesis, inflammation and apoptosis in hepatocytes. Transmission Electron Microscope (TEM) imaging showed that 4-PBA treatment mitigated the ER swelling induced by OA. These results were confirmed in the in vivo experiment, where 4-PBA injection reduced lipid deposition and expression levels of genes/proteins related to ERS, lipogenesis, inflammation and apoptosis in liver compared to the HFD treatment. In conclusion, these results confirmed that ERS plays a contributing regulatory role in HFD- and OA-induced lipotoxic damage including lipid accumulation, apoptosis and inflammation in liver and isolated hepatocytes of black seabream, which supply a new perspective in the cross-talk of ERS and hepatic lipotoxic damage in hepatocytes/liver of marine fish and provide theoretical guidance for the prevention and treatment of hepatic lipotoxic damage.

Anthracycline-induced cardiotoxicity – primary preventive options. An overview

Anthracycline-induced cardiotoxicity (AIC) has been known since the 1970s but remains relevant, mainly due to the widespread use of anthracyclines in oncological practice. Currently, AIC is viewed as a prolonged and continuous process that starts with the initial dose at molecular and cellular levels and advances, ultimately resulting in cardiomyopathy and heart failure. Many contemporary studies aim to discover methods for early detection of anthracycline-related myocardial injury. Additionally, ongoing research focuses on agents for primary prevention of AIC, examining their effectiveness, place, and timing of administration. This review aims to present the available preventive options, interconnecting them with their mechanisms of action and the mechanisms of AIC. According to current recommendations, primary prevention is indicated for high- and very high-risk patients and includes general measures to lower cardiovascular risk, neurohormonal therapy, and statins. Regular cardiology assessments are a crucial component of heart damage prevention. Evidence of mild myocardial injury (deteriorated Global Longitudinal Strain and troponin release) justifi es cardioprotective measures. Some aspects remain unanswered, including whether early cardioprotection is required, as well as the role and effi cacy of sacubitril/valsartan and sodium-glucose cotransporter 2 inhibitors (SGLT2 inhibitors) for AIC prevention. Physical activity is a promising method to reduce the cardiac damaging effects of anthracyclines. 

Laboratory exploration of a novel method to protect silicate relics against salt efflorescence by directional induction of water

Salt efflorescence is one of the critical problems for the preservation of immovable silicate relics. Salt efflorescence mainly comes from continuous cycles of crystallization/dissolution or hydration/dehydration of salts in confined pores in silicate relics. Many protocols have been developed in attempts to alleviate possible salt damages with minor success because of endless water and salt feed from underground. In this study, we propose and design a novel technique for salt damage prevention and protection of immovable relics. Materials with higher water-absorbing ability than matrix are applied to control the water and salt migration direction in simulated sand samples. The distribution of moisture content on the surface of sand is followed by hyperspectral imaging. It appears that water and salt molecules will preferentially transport towards positions containing higher water-absorbing material. Both organic and inorganic high water-absorbing materials show effective in controlling the water and salt migration direction, which provides a new approach for the prevention and protection of salt efflorescence in silicate cultural relics.

Research progress on the prevention and treatment of chemotherapy-induced ovarian damage.

Chemotherapy is a main treatment option for malignant tumors, but it may cause various adverse effects, including dysfunction of female endocrine system and fertility. Chemotherapy-induced ovarian damage has been concerned with ovarian preservation but also the prevention and treatment of ovarian dysfunction. In this article, the mechanisms of ovarian injury caused by chemotherapy, including apoptosis of the follicle and supporting cells, follicle "burn out", ovarian stromal and microvascular damage; and influencing factors, including age at diagnosis, initial low pre-treatment anti-Müllerian hormone levels, toxicity, dose and regimen of chemotherapy drugs are reviewed based on the latest research results and clinical practice. The article also discusses measures and frontier therapies for the prevention and treatment of ovarian injury, including the application of gonadotropin releasing hormone agonists or antagonists, tyrosine kinase inhibitors, antioxidants, sphingosine-1-phosphate, ceramide-1-phosphate, mammalian target of rapamycin inhibitors, granulocyte-colony stimulating factor, stem cell therapy and artificial ovaries.

Cuproptosis is involved in decabromodiphenyl ether-induced ovarian dysfunction and the protective effect of melatonin

Decabromodiphenyl ether (BDE-209) has been universally detected in environmental media and animals, but its damage to ovarian function and mechanism is still unclear, and melatonin has been shown to improve mammalian ovarian function. This study aimed to investigate the toxic effects of BDE-209 on the ovary and tried to improve ovarian function with melatonin. Herein, BDE-209 was administered orally to female SD rats for 60 days. Enzyme-linked immunosorbent assay, HE staining, transcriptome analysis, qPCR and immunohistochemical staining were used to explore and verify the potential mechanism. We found that BDE-209 exposure had effects on the ovary, as shown by abnormal changes in the estrous cycle, hormone levels and ovarian reserve function in rats, while increasing the proportion of collagen fibres in ovarian tissue. In terms of mechanism, cuproptosis, a form of cell death, was identified to play a crucial role in BDE-209-induced ovarian dysfunction, with the phenotype manifested as copper salt accumulation in ovary, downregulation of glutathione pathway metabolism and copper transfer molecule (ATP7A/B), and upregulation of FDX1, lipoic acid pathway (LIAS, LIPT1), pyruvate dehydrogenase complex components (DLAT, PDHB, PDHA1), and copper transfer molecule (SLC31A1). Furthermore, possible interventions were explored. Notably, a supplement with melatonin has a repair effect on the damage to ovarian function by reversing the gene expression of cuproptosis-involved molecules. Overall, this study revealed that cuproptosis is involved in BDE-209-induced ovarian damage and the beneficial effect of melatonin on ovarian copper damage, providing evidence for the prevention and control of female reproductive damage induced by BDE-209.

Analysis of the role of local government in the prevention of environmental damage in Kuala Tanjung Industrial Park, North Sumatra, Indonesia

The development of active industrial areas in the production process of course produces waste that can damage the surrounding environment. Kuala Tanjung Village is a Priority Industrial Area based on Presidential Regulation Number 56 of 2018. A review of environmental impacts needs to be carried out. Active local government participation is needed, of course through regulation. This research aims to examine the efforts made by the Government of Batu Bara Regency to prevent environmental damage to the industrial area in Kuala Tanjung village. The research method used is a qualitative method with a descriptive type of research. While the data used are primary data and secondary data. The result showed that the Regional Government of Batubara Regency has Regional Regulation Number 2 of 2022 concerning the implementation of environmental protection and management. However, it is a pity that the regulation has just been issued, even though several companies have been operating for a long time. Furthermore, there are also programs and activities carried out, namely monitoring river water, routine monitoring of companies, and planting mangrove forests. The entire program is still limited to liquid waste, while air quality is far from being a concern. Coupled with the lack of budget allocations, the programs that are run are still very limited.

Analysis of the role of local government in the prevention of environmental damage of Lake Toba, North Sumatra, Indonesia

This study aims to investigate the evaluation of the Lake Toba Priority Tourism Master Plan (LTPTMP) for the year 2022. Employing a qualitative research design, the research utilizes a thematic approach. Data collection involves interviews, observations, focus group discussions (FGDs), and document studies. The selection of informants is done through purposive sampling. Thematic analysis is employed for data analysis. Findings indicate that key stakeholders, such as local governments and ministries, exhibit a good understanding and implementation of the LTPTMP. However, the community and Micro, Small, and Medium Enterprises (MSMEs) in the Lake Toba region lack awareness and knowledge regarding the LTPTMP. Challenges in implementing the plan include issues like lake water pollution, deforestation, the presence of waste, especially in tourist-frequented areas, low public understanding and awareness of resource conservation, and inadequate management by the Regional Government. Furthermore, the incomplete infrastructure network hampers visitors’ ability to explore various sites.

Using a frequency vibration approach, examine the impact of screw rotor clearance on the screw housing in an 11kW compressor

A screw compressor is one of the industrial machines needed for productivity and use in today's production. Industrial machines generally use pneumatic systems with the help of compressed air supplied by a compressor machine. The compressor working system must receive very special attention. Maintenance and prevention of damage to air screw compressor machines is very necessary, especially maintenance and prevention of damage to rotary screw air compressors. Problems that often occur with the shaft will result in reduced air supply flow or unstable air pressure resulting in disruption of the production line. The aim of this research was to discuss the vibration values produced against the rotor clearance from the ideal output on male and female screw compressor rotors using the frequency analysis method, taking the vibration values at the screw compressor housing point using an Accelerometer sensor and a Fast Fourier Transform (FFT) tool. The analyzer rotates at 1500 rpm in the X, Y and Z axes at each predetermined point. Research shows that the clearance is set at 0.03, 0.05 and 0.08. In this research, the high frequency sample values of 1x 25 Hz, 2x 50 Hz, and 3x 75 Hz often appear in each vibration data of 0.03 and 0.05, at the vibration frequency range value of 0.08 none appear. This shows the results that the ideal rotor clearance value is at a distance of 0.08 because there is no frequency graph of 1x, 2x, and 3x rpm at each point and axis on the end housing of the screw rotor compressor

Determination of Critical Damage Size of Inclined Waterproof Coal Pillar under Asymmetric Load

Quantitative determination of the critical size of an inclined coal pillar in an old goaf water-affected area is of great significance for water damage prevention and safe mining. The critical size of the inclined waterproof coal pillar is derived by using mechanical analyses, numerical calculations, and field engineering practices to determine the stability of the waterproof coal pillar in the old goaf water-affected area of the 1303 working face of Dananhu No. 1 Mine in the Xinjiang region. Firstly, a force model of the inclined waterproof coal pillar was established to reveal the law that the critical size of the coal pillar increases with the increase in coal seam inclination under the action of asymmetric load. Then, numerical simulation was applied to reveal the dynamic evolution processes of plastic deformation–destabilization of the coal pillar under the influence of mining and single-side water pressure, and the critical size of the coal pillar in the study area was determined to be 19.09 m. Finally, measures such as pumping pressure relief and slurry reinforcement were adopted to reduce the deformation rate of the roadway on the side of the coal pillar, which ensured the stability of the waterproof coal pillar and the safe mining of the working face.

Targeted treatments for immune dysregulation in inborn errors of immunity

Primary immune regulatory disorders (PIRDs) constitute a subset of inborn errors of immunity and are characterized by lymphoproliferation, autoimmunity, malignancy, and infection. Unlike classical primary immune deficiencies, initial symptoms of PIRDs can manifest as autoimmunity such as cytopenias or enteropathy, which can often prove resistant to conventional treatments and occur years prior to the onset of infectious complications. Raising awareness about PIRDs among specialists and adopting a multidisciplinary approach is crucial for early diagnosis, intervention, and potential prevention of severe organ damage. Significant progress has been made in identifying several PIRDs, which has contributed to a more comprehensive comprehension of their underlying immunological mechanisms. This knowledge has paved the way for targeted therapies focusing on specific molecules, which tend to offer superior disease control compared to traditional immunosuppressants. This review, informed by the latest literature, explores prevalent PIRDs, detailing their clinical manifestations and recent advancements in treatment modalities.

A quantitative and qualitative literature review of water damage in buildings occurring in building service systems, appliances and wet rooms

Water damage affects both residents and buildings. Issues include moisture, climate impact and repair costs that amount to immense resources. Because of these, immense resources research is important to undertake to reduce and limit the extent and effects of water damage. This study aimed to compile and review the literature on water damage in buildings. Literature distribution, the status, consequences, effects, and strategies and methods for prevention of water damage were assessed by compiling and reviewing literature using quantitative and qualitative methods. Two scientific databases were searched to identify the relevant literature. Key findings of this study are that research conducted on water damage is embedded in the study of building defects and building pathology, and more specific research on water damage is needed depending on different rooms and buildings, the effect of moisture and sustainability. Informed and guided decisions, management and organizational structures have been internationally suggested as a part of the solution to reducing the amount and effect of defects and could have the possibility to reduce the amount and effect of water damage. However, there is a gap in research on water damage consequences, occurrence and costs in Nordic conditions.

The legal framework of the European Union in the field of combating environmental crimes

The article focuses on the activities of the European Union regarding the development of an effective legal framework for combating environmental crimes considering modern realities. It is noted that the existing norms on combating environmental crime in the EU are based on the provisions of the Treaty on the Functioning of the EU which sets the competence of the EU to define criminal offenses and sanctions in the spheres of EU policy to which harmonization measures apply (Article 83(2)). Attention is paid to the provisions of the following directives: Directive of the European Parliament and of the Council on the protection of the environment through criminal law and replacing Directive 2008/99/EC, which supplemented the list of illegal acts with new categories of crimes, established a deterrent level of sanctions, which ensures a more consistent application of conditions for enterprises in the EU; Directive 2004/35/CE of the European Parliament and of the Council of 21 April 2004 on environmental liability with regard to the prevention and remedying of environmental damage, which sets rules based on the «polluter pays» principle and related directives 2000/60/EC, 2008/56/EC, 2009/147/EC and 92/43/EEC. It is noted that the EU constantly works on improving the provisions of the acts adopted by it in the specified area, as evidenced by the directives, on the basis of which changes and amendments were made to the above-mentioned directives, regarding safety issues, waste management, simplification of reporting obligations in the field of environmental legislation (Directives 2006/21/EC, 2009/31/EC, 2013/30/EC, Regulation (EC) 2019/101).
 Attention is focused on acts related to the protection of certain objects, including: 1) acts aimed at combating pollution from ships: Directive 2005/35/EC of the European Parliament and of the Council of 7 September 2005 on ship-source pollution and on the introduction of penalties, including criminal penalties, for pollution offence (as amended), Council Framework Decision 2005/667/JHA of 12 July 2005 to strengthen the criminal-law framework for the enforcement of the law against ship-source pollution; 2) acts on combating trade in wild animals: Regulation (EC) No 338/97 (as amended), Commission regulation (EC) No 865/2006 (as amended), Commission Implementing Regulation (EU) No 792/2012 97 (as amended), Revision of the EU action plan against wildlife trafficking of 2022.
 It is noted that EU legislation is based on international universal acts adopted within the UN in the relevant field (International Convention for the Prevention of Pollution from Ships (MARPOL) of 1973, United Nations Convention on the Law of the Sea (UNCLOS) of 1982, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) of 1975), which simplifies law enforcement and the introduction of measures to combat environmental crime.
 The importance of strategic acts adopted by the EU, including the Agenda and Action Plan on Drugs 2021–2025 and the Biodiversity strategy for 2030 is emphasized. Relevant conclusions have been drawn.