Prevent Hair Loss Research Articles

Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction.

Coffee has been a common ingredient in many traditional hair loss remedies, but limited scientific evidence supports its use, particularly in coffee pulp. Androgenetic alopecia (AGA) is caused by androgens, inflammation, and oxidative stress. In the present study, supercritical fluid extraction (SFE) was used under various conditions to obtain six coffee pulp extracts. The SFE-4 extract, using 50% (v/v) ethanol as a co-solvent at conditions of 100 °C and 500 bars for 30 min, exhibited the highest phenolic, flavonoid, and caffeine contents. Additionally, the SFE-4 extract increased the migration and cell proliferation of HFDPCs (human hair follicle dermal papilla cells), which control hair cycle regulation, and had scavenging effects on ABTS and DPPH radicals. Additionally, the SFE-4 extract showed potassium ion channel opener activity in HFDPCs, as well as a stimulation effect on the enzyme matrix metalloproteinase-2 (MMP-2) (28.53 ± 1.08% of control), which may be related to the vascular endothelial growth factor (VEGF) gene upregulation. In human prostate cancer cells (DU-145) and HFDPC cells, the SFE-4 extract significantly decreased the expression of SRD5A1, SRD5A2, and SRD5A3, an essential pathway involved in AGA. Hair growth factor genes in the Wnt/-catenin (CTNNB1) and Sonic Hedgehog (SHH, SMO, and GLI1) pathways could be significantly activated by the SFE-4 extract. These results imply that employing SFE in coffee pulp extraction could help AGA treatment by preventing hair loss and promoting hair growth pathways. This would help small coffee producers gain economic empowerment and ensure the long-term sustainability of agricultural waste utilization.

Bioactive Compound-Loaded Nanocarriers for Hair Growth Promotion: Current Status and Future Perspectives.

Hair loss (alopecia) has a multitude of causes, and the problem is still poorly defined. For curing alopecia, therapies are available in both natural and synthetic forms; however, natural remedies are gaining popularity due to the multiple effects of complex phytoconstituents on the scalp with fewer side effects. Evidence-based hair growth promotion by some plants has been reported for both traditional and advanced treatment approaches. Nanoarchitectonics may have the ability to evolve in the field of hair- and scalp-altering products and treatments, giving new qualities to hair that can be an effective protective layer or a technique to recover lost hair. This review will provide insights into several plant and herbal formulations that have been reported for the prevention of hair loss and stimulation of new hair growth. This review also focuses on the molecular mechanisms of hair growth/loss, several isolated phytoconstituents with hair growth-promoting properties, patents, in vivo evaluation of hair growth-promoting activity, and recent nanoarchitectonic technologies that have been explored for hair growth.

Ethnopharmacology Study of Traditional Herbs as Anti-dandruff and Anti-baldness in Amarasi District, Kupang Regency

Backgrounds: Hot weather in Indonesia can cause problems with hair and scalp health, including dandruff (seborrheic) and cause hair roots to become weak, making it easier for hair loss or alopecia to occur. The Amarasi people still do hair and scalp treatments with traditional plants. Objectives: This study aims to determine the ethnopharmacology of herbs as anti-dandruff and anti-baldness in Amarasi sub-district, Kupang district, including plant names, plant properties, plant parts, processing methods, usage methods and usage rules. Methods: This research is a qualitative descriptive study with an exploratory observational design. The data was obtained in the form of a survey by interviewing respondents with the help of a questionnaire. The sample in this study was a traditional healer (Hatra) who understood the use and use of anti-dandruff and anti-baldness drugs. Sampling was carried out using a purposive sampling techniq namely Hatra who were over 20 years old and had experience as a hatred for more than 3 years and above. The sample in this study was 17 traditional healers (Hatra). Results: result research shows that there are 18 plants consisting of 8 single plants and 16 herbs that are used and efficacious as anti-dandruff and anti-baldness. The plant parts used are leaves, fruit, seeds, sap/mucus, tube and oots. the method of processing plants for hair and scalp care by burning, splitting, fryinsqueezing and g, mashing, then, how to use it is washed, smearing, washedmassagingsaged. Rules for using plants 1-2 times a week. Conclusion: The Amarasi community has traditional plants and herbs that can be used as a treatment for scalp problems, including anti-dandruff, preventing hair loss and nourishing hair (anti-baldness). These plants based on research literacy studies conducted by previous researchers proved to have potential in the treatment of anti-dandruff and antibody.

Practical Guideline for Prevention of Patchy Hair Loss following CyberKnife Stereotactic Radiosurgery for Calvarial or Scalp Tumors: Retrospective Analysis of a Single Institution Experience

Introduction: Patchy alopecia is a common adverse effect of stereotactic radiosurgery (SRS) on the calvarium and/or scalp, yet no guidelines exist for its prevention. This study aims to investigate the incidence and outcomes of patchy alopecia following SRS for patients with calvarial or scalp lesions and establish preventive guidelines. Methods: The study included 20 patients who underwent CyberKnife SRS for calvarial or scalp lesions, resulting in a total of 30 treated lesions. SRS was administered as a single fraction for 8 lesions and hypofractionated for 22 lesions. The median SRS target volume was 9.85 cc (range: 0.81–110.7 cc), and the median prescription dose was 27 Gy (range: 16–40 Gy), delivered in 1–5 fractions (median: 3). The median follow-up was 15 months. Results: Among the 30 treated lesions, 11 led to patchy alopecia, while 19 did not. All cases of alopecia resolved within 12 months, and no patients experienced other adverse radiation effects. Lesions resulting in alopecia exhibited significantly higher biologically effective dose (BED) and single-fraction equivalent dose (SFED) on the overlying scalp compared to those without alopecia. Patients with BED and SFED exceeding 60 Gy and 20 Gy, respectively, were 9.3 times more likely to experience patchy alopecia than those with lower doses. The 1-year local tumor control rate for the treated lesions was 93.3%. Chemotherapy was administered for 26 lesions, with 11 lesions receiving radiosensitizing agents. However, no statistically significant difference was found. Conclusion: In summary, SRS is a safe and effective treatment for patients with calvarial/scalp masses regarding patchy alopecia near the treated area. Limiting the BED under 60 Gy and SFED under 20 Gy for the overlying scalp can help prevent patchy alopecia during SRS treatment of the calvarial/scalp mass. Clinicians can use this information to inform patients about the risk of alopecia and the contributing factors.

Simultaneous Determination of Three Flavonoids in Rat Plasma of Masson Pinus Needles (Pinus massoniana L.) Extracts by UHPLC-MS/MS and Application to Pharmacokinetics Studies after Oral and Transdermal Administration.

Pinus massoniana needles, a traditional herb, were applied to prevent hair loss in China. Studies available mainly focused on pine needle flavonoids with various biological activities. However, there has been no pharmacokinetics study of the flavonoids from Pinus needles extract. A selective and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously quantify taxifolin, quercetin and catechin in rat plasma. To separate the three constituents, an Agilent Extend-C18 column (2.1mm × 100mm, 1.8μm) was used with a mobile phrase of (A) 0.1% formic acid and (B) acetonitrile. The analytes were measured by multiple reaction monitoring in the negative ionization mode. There was good linearity in the established UHPLC-MS/MS method, with a coefficient of determination (r2) of >0.99. The accuracy, intra-day and inter-day precision and recovery were all satisfactory and these 3 compounds were stable under the tested conditions. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral and transdermal administration of Pinus needles extract. The results could provide further research foundation for pine needle extract as external preparations.

Formulation and evaluation of herbal hair serum-a review

In the mammalian system the hair follicle is known to the most significant organ that determine, appearance, gender distinction, provides intense temperature protection and plays a role in self-defence. The younger generations have begun to suffer extreme hair loss problem due to many reasons. The hair loss is not temporary in most cases. but it results in alopecia. Many people suffering from hair loss is in search of multiple treatments due to extreme anxiety and tension. To improve hair growth and to prevent hair loss, hair root activation is required. Citrus sinensis is used to antidandruff protection hair care. It’s had antibacterial and anti-inflammatory properties. Nigella sativa improves the shine, strength, volume, and texture of hair. Flax seed is full of fatty acids and antioxidants that tend to remove pollutants and dead cells from the scalp. Coconut oil may help to moisturise and seal hair. They can help to prevent dry, flaky scalp and dandruff. This review article focuses on the materials required for the formulation of hair serum and various evaluation tests to evaluate hair serum.

Rosmarinus officinalis Essential Oil Increases Hair Length and Follicle Diameter of Ultraviolet B-exposed Mice Through VEGF

BACKGROUND: Ultraviolet (UVB) radiation induces hair photoaging by triggering oxidative stress, causing alterations in hair diameter and length, which contribute to the development of senile alopecia. Development of alternative anti-photoaging agents to prevent hair loss from various materials, including natural products, is currently being explored. Rosmarinus officinalis essential oil (ROEO) has been known to have antioxidant and vasodilation properties. However, the effect and mechanism of ROEO on UVB-exposed hair are still unclear. Therefore, this study was conducted to evaluate the effects of ROEO on the hair length and follicle diameter in UVB-exposed mice as well as the skin vascular endothelial growth factor (VEGF) level.METHODS: Thirty male Swiss mice were treated topically with/without paraffin oil, 2% minoxidil, or various concentrations of ROEO, every day for 21 days. Meanwhile, UVB exposure was performed 3×/week. On day 21, the hair length was measured, the skin tissue was collected for hair follicle diameter and VEGF measurements.RESULTS: ROEO contained phenolic, including flavonoids and tannins, as well as non-phenolic antioxidants, including 1.8-cineole, α-pinene, and camphor. The IC50 value of ROEO was 15.977 ppm. Significant higher hair length, follicle diameter, and VEGF level of 10% ROEO+UVB-treated mice were observed, compared with the ones of mice exposed with UVB merely (LSD test, p<0.05).CONCLUSION: Since 10% ROEO could significantly increase hair length, follicle diameter and VEGF level, and contained antioxidant compounds, it can be suggested that ROEO might increase hair length, follicle diameter, and VEGF level through its antioxidant component.KEYWORDS: Rosmarinus officinalis, hair follicle diameter, hair length, VEGF, ultraviolet B, antioxidant

The development of non-scarring alopecia in women who wear the hijab.

Little is known about hair loss associated with wearing the hijab, a religious head covering worn by Muslim women. We performed a single-center analysis to investigate the association between various forms of non-scarring alopecia and wearing the hijab. This study included 125 patients who wore the hijab and 40 race/ethnicity-matched women who did not wear the hijab. Among the 165 total patients diagnosed between January 2015 and March 2022, 71 had telogen effluvium, 78 had female pattern hair loss, and 16 had traction alopecia. We found patients who wore the hijab had a younger mean age of alopecia onset than patients who did not wear the hijab (31.5 vs. 37.3years; P = 0.02). Our study suggests that vitamin D deficiency (OR 4.1; 95% CI 1.2-14.1; P = 0.02) and seborrheic dermatitis (OR 2.9; 95% CI 1.1-8.1; P = 0.03) may significantly impact the development of telogen effluvium in patients who wear the hijab. Targeting these risk factors among patients who wear the hijab may be considered to prevent hair loss.

LB1680 Effects of ganoderma lucidum extract and its active component, ganoderic acid A on hair loss prevention

LB1680 Effects of ganoderma lucidum extract and its active component, ganoderic acid A on hair loss prevention

Potential Natural Products Regulation of Molecular Signaling Pathway in Dermal Papilla Stem Cells.

Stem cells have demonstrated significant potential for tissue engineering and repair, anti-aging, and rejuvenation. Hair follicle stem cells can be found in the dermal papilla at the base of the follicle and the bulge region, and they have garnered increased attention because of their potential to regenerate hair as well as their application for tissue repair. In recent years, these cells have been shown to affect hair restoration and prevent hair loss. These stem cells are endowed with mesenchymal characteristics and exhibit self-renewal and can differentiate into diverse cell types. As research in this field continues, it is probable that insights regarding stem cell maintenance, as well as their self-renewal and differentiation abilities, will benefit the application of these cells. In addition, an in-depth discussion is required regarding the molecular basis of cellular signaling and the influence of nature-derived compounds in stimulating the stemness properties of dermal papilla stem cells. This review summarizes (i) the potential of the mesenchymal cells component of the hair follicle as a target for drug action; (ii) the molecular mechanism of dermal papilla stem cells for maintenance of their stem cell function; and (iii) the positive effects of the natural product compounds in stimulating stemness in dermal papilla stem cells. Together, these insights may help facilitate the development of novel effective hair loss prevention and treatment.

Follicular Delivery of Caffeine from a Shampoo for Hair Retention

A key factor in the prevention of hair loss is the provision of optimal conditions on the scalp. In this regard, reduction of oxidative stress on the scalp is one critical requirement to support the hair follicles to function optimally. Recently, a novel shampoo formulation technology containing anti-oxidants such as piroctone olamine has been demonstrated to improve hair retention based on micellar degradation and coacervation effects. Caffeine has also been shown to exhibit anti-oxidant activity including the ability to inhibit lipid peroxidation. As with piroctone olamine, it is expected that follicular delivery of caffeine will enhance its anti-oxidant activity in a region that will be beneficial for hair retention. In this study, two shampoo formulations as well as a control formulation were applied to the calf area of n = 9 male participants. The technique of differential tape stripping was applied to obtain the caffeine penetrated to the stratum corneum and to the hair follicles. Isotope-dilution liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was performed to demonstrate caffeine follicular delivery from the shampoo formulas. The results showed that the percentage of caffeine recovered in the hair follicles was 8–9% of the caffeine absorbed into the skin and matched an existing caffeine-based shampoo. In conclusion, a novel shampoo formulation technology has been developed that effectively delivers beneficial anti-oxidants to improve hair retention. This new shampoo is expected to be especially useful in the goal of retaining hair during aging.

Enhancing Hairfall Prediction: A Comparative Analysis of Individual Algorithms and An Ensemble Method

Hair fall, a prevalent issue affecting many individuals globally, necessitates early detection for preventive measures and hair health maintenance. Machine learning algorithms have gained attention in predicting hair fall by analysing genetic predisposition, lifestyle habits, and environmental factors. However, the performance of individual algorithms can be improved through ensemble models that combine their strengths. This research paper proposes an ensemble machine learning approach tailored for hair fall prediction. Comparative evaluations with individual algorithms reveal the ensemble models consistently outperform in accuracy, precision, and recall. Leveraging diverse algorithms, the ensemble approach captures a wider range of hair fall patterns, enhancing prediction accuracy. The ensemble models also exhibit higher precision and recall rates, correctly identifying both hair fall and non-hair fall instances. The ensemble models' superiority stems from mitigating the limitations of individual algorithms, resulting in a comprehensive and robust prediction framework. Overall, this research showcases the efficacy of ensemble machine learning models in hair fall prediction, enabling early detection and intervention for hair loss prevention. These findings provide valuable insights for researchers, practitioners, and individuals concerned about hair health.

Ethnopharmacology Study of Traditional Herbs as Anti-dandruff and Anti-baldness in Amarasi District, Kupang Regency

Backgrounds: Hot weather in Indonesia can cause problems with hair and scalp health, including dandruff (seborrheic) and cause hair roots to become weak, making it easier for hair loss or alopecia to occur. The Amarasi people still do hair and scalp treatments with traditional plants. Objectives: This study aims to determine the ethnopharmacology of herbs as anti-dandruff and anti-baldness in Amarasi sub-district, Kupang district, including plant names, plant properties, plant parts, processing methods, usage methods and usage rules. Methods: This research is a qualitative descriptive study with an exploratory observational design. The data was obtained in the form of a survey by interviewing respondents with the help of a questionnaire. The sample in this study was a traditional healer (Hatra) who understood the use and use of anti-dandruff and anti-baldness drugs. Sampling was carried out using a purposive sampling techniq namely Hatra who were over 20 years old and had experience as a hatred for more than 3 years and above. The sample in this study was 17 traditional healers (Hatra). Results: result research shows that there are 18 plants consisting of 8 single plants and 16 herbs that are used and efficacious as anti-dandruff and anti-baldness. The plant parts used are leaves, fruit, seeds, sap/mucus, tube and oots. the method of processing plants for hair and scalp care by burning, splitting, fryinsqueezing and g, mashing, then, how to use it is washed, smearing, washedmassagingsaged. Rules for using plants 1-2 times a week. Conclusion: The Amarasi community has traditional plants and herbs that can be used as a treatment for scalp problems, including anti-dandruff, preventing hair loss and nourishing hair (anti-baldness). These plants based on research literacy studies conducted by previous researchers proved to have potential in the treatment of anti-dandruff and antibody.

Millet seed oil activates β-catenin signaling and promotes hair growth.

Alopecia, regardless of gender, exacerbates psychological stress in those affected. The rising prevalence of alopecia has fueled a research interest in preventing hair loss. This study investigates the potential of millet seed oil (MSO) in promoting the proliferation of hair follicle dermal papilla cells (HFDPC) and stimulating hair growth in animals with testosterone-dependent hair growth inhibition as part of a study on dietary treatments to improve hair growth. MSO-treated HFDPC significantly increased cell proliferation and phosphorylation of AKT, S6K1, and GSK3β proteins. This induces β-catenin, a downstream transcription factor, to translocate to the nucleus and increase the expression of factors related to cell growth. In a C57BL/6 mice model in which hair growth was inhibited by subcutaneous testosterone injection after shaving the dorsal skin, oral administration of MSO stimulated hair growth in the subject mice by increasing the size and number of hair follicles. These results suggest that MSO is a potent agent that may help prevent or treat androgenetic alopecia by promoting hair growth.

Innovative Strategies for Hair Regrowth and Skin Visualization.

Today, about 50% of men and 15-30% of women are estimated to face hair-related problems, which create a significant psychological burden. Conventional treatments, including drug therapy and transplantation, remain the main strategies for the clinical management of these problems. However, these treatments are hindered by challenges such as drug-induced adverse effects and poor drug penetration due to the skin's barrier. Therefore, various efforts have been undertaken to enhance drug permeation based on the mechanisms of hair regrowth. Notably, understanding the delivery and diffusion of topically administered drugs is essential in hair loss research. This review focuses on the advancement of transdermal strategies for hair regrowth, particularly those involving external stimulation and regeneration (topical administration) as well as microneedles (transdermal delivery). Furthermore, it also describes the natural products that have become alternative agents to prevent hair loss. In addition, given that skin visualization is necessary for hair regrowth as it provides information on drug localization within the skin's structure, this review also discusses skin visualization strategies. Finally, it details the relevant patents and clinical trials in these areas. Together, this review highlights the innovative strategies for skin visualization and hair regrowth, aiming to provide novel ideas to researchers studying hair regrowth in the future.

OPTIMASI FORMULA HAIR TONIC MIKROEMULSI BIOTIN (VITAMIN B7) DENGAN METODE FACTORIAL DESIGN

Loss becomes a problem in the hair that causes baldness. Biotin is a vitamin that nourishes hair and prevents hair loss. Biotin which is formulated in microemulsion tends to be transparent, has low viscosity and high solubility. Formula optimization was carried out to determine the concentration of span 80, tween 80, and PEG 400 in biotin microemulsion hair tonic. The research was conducted on an experimental basis using a factorial design method with 3 factors, namely span 80, tween 80, and PEG 400. The optimum microemulsion formula was determined using Design Expert 13 software using the Factorial Design method. Testing the physical quality of the preparation is carried out at the optimum formula. Optimization of biotin microemulsion hair tonic showed results of span 80 (20%), tween 80 (9.466%) and PEG 400 (5%) with a predicted viscosity of 46.75 cPs and globule size of 2.98 µm. Prediction testing and test results compared to the One Sample T-test showed results that were not significantly different with a significance value of >0.05. The optimum formula has a viscosity value of 47.67 cPs and a globule size of 2.4 µm. The results obtained concluded that the optimal composition composition of the biotin microemulsion formula from the combination of span 80, tween 80 and PEG 400 which produces hair tonic with good effects and physical quality is span 80 20%, tween 80 9.47% and PEG 400 5%.

Prevention and Treatment of Chemotherapy-Induced Alopecia: What Is Available and What Is Coming?

Millions of new cancer patients receive chemotherapy each year. In addition to killing cancer cells, chemotherapy is likely to damage rapidly proliferating healthy cells, including the hair follicle keratinocytes. Chemotherapy causes substantial thinning or loss of hair, termed chemotherapy-induced alopecia (CIA), in approximately 65% of patients. CIA is often ranked as one of the most distressing adverse effects of chemotherapy, but interventional options have been limited. To date, only scalp cooling has been cleared by the US Food and Drug Administration (FDA) to prevent CIA. However, several factors, including the high costs not always covered by insurance, preclude its broader use. Here we review the current options for CIA prevention and treatment and discuss new approaches being tested. CIA interventions include scalp cooling systems (both non-portable and portable) and topical agents to prevent hair loss, versus topical and oral minoxidil, photobiomodulation therapy (PBMT), and platelet-rich plasma (PRP) injections, among others, to stimulate hair regrowth after hair loss. Evidence-based studies are needed to develop and validate methods to prevent hair loss and/or accelerate hair regrowth in cancer patients receiving chemotherapy, which could significantly improve cancer patients' quality of life and may help improve compliance and consequently the outcome of cancer treatment.

Hair growth-promotion effects and antioxidant activity of the banana flower extract HappyAngel®: double-blind, placebo-controlled trial

Banana flowers contain various bioactive components, including several antioxidants with anti-inflammatory effects. However, it is unclear whether they can reduce and prevent hair loss. This study examines the effect of banana flower extracts on preventing hair loss and strengthening hair roots. The banana flower extract (HappyAngel®) was used to treat human hair follicle dermal papilla cells (HFDPCs) and the expression of reactive oxygen species (ROS), dihydrotestosterone (DHT), and hair-related genes (SRD5A1, SRD5A2, AR, and KROX20) were monitored. Fifty subjects were divided into a placebo group and a banana flower group. The experimental group consumed banana flower extract daily for twelve weeks and then underwent hair testing, hair-related genes analysis, collection of hair loss, and questionnaires. The results showed that the banana flower extract significantly increased hair cell growth and decreased the expression of ROS, DHT, and hair follicle growth inhibition-related SRD5A1, SRD5A2, and AR genes, and significantly increased the expression of hair growth-related KROX20 gene in HFDPCs. Consuming banana flower extract for twelve weeks increased the hair root diameter and reduced hair loss and scalp redness compared to the placebo group. Thus, banana flower extract (HappyAngel®) can stimulate hair growth and inhibit the activation of hair loss genes.

Comparative studies of the specific activity of soft dosage forms minoxidil for trichological practice

The treatment and prevention of hair loss have been a serious aesthetic problem for the population for a long time due to the widespread occurrence of the condition and its undesirable medical and social consequences. The pharmacotherapeutic options for trichological practice are very limited, and the effectiveness of many of these drugs is small or questionable. The peripheral vasodilator minoxidil is one of the few medicinal substances that has been proven to stimulate hair growth. However, the number of minoxidil preparations allowed by the Ministry of Health for use in Ukraine is minimal and mainly represented by rare dosage forms for external use. Considering the above, the employees of the Department of Medicines Technology at Zaporizhzhia State Medical University have proposed rational formulations and technologies for two new soft dosage forms of minoxidil – a 2 % cream for external use based on an emulsion, and a shampoo in gel form – based on complex physical-chemical, pharmaco-technological, rheological, and biopharmaceutical research. These formulations are intended to stabilize hair growth and restore hair coverage in men and women suffering from androgenic alopecia. The aim of this research is to study the specific activity of the proposed topical dosage forms of minoxidil. Materials and methods. Experimental cream with 2 % minoxidil on an emulsion carrier and a gel-like shampoo containing 1 % minoxidil combined with phytoextracts of hops and burdock, as well as corresponding placebo compositions, were used as objects of preclinical studies. The compositions of the topical minoxidil pharmacotherapeutic agents are scientifically based, ensuring their optimal pharmacotechnological, biopharmaceutical, and consistent consumer properties. The study of the specific activity of soft medicinal forms of minoxidil for external use was carried out by evaluating their growth-stimulating and follicle-protective activity. “Pilfood Bosnalek” spray for external use (Bosnalijek, Bosnia and Herzegovina), containing 2 % minoxidil, was used as a reference drug when studying the growth-stimulating effect of 2 % cream for external use with minoxidil. The reference drug in the study of the similar activity of the gel-like shampoo “Trichlomine” was the shampoo against hair loss “Dove” (Unilever, Germany). Results. It has been found that a 2 % minoxidil cream for topical use, when used together with the reference drug “Pilfood Bosnalek” (Bosnalijek, Bosnia and Herzegovina), has a significant hair growth-stimulating effect, which statistically significantly increases hair growth in experimental animals from as early as 3–4 days of use. The shampoo “Trichlomine” also has a significant effect on hair growth in experimental animals, with results observed from 3–4 days of use. Furthermore, the strength of the hair growth-stimulating effect observed from the use of “Trichlomine” shampoo significantly exceeds that of the reference “Dove” shampoo (Unilever, Germany). These results demonstrate the presence of significant follicle-stimulating activity in the tested minoxidil preparations for treating experimental alopecia and their positive effects on the weight and structure of laboratory animal fur. The follicle-protective activity of experimental minoxidil dosage forms (2 % cream and gel-like shampoo) was also experimentally confirmed, due to the regulation of various metabolic pathways in the dermis. The use of 2 % minoxidil cream and comparison preparations (“Dove” shampoo and “Pilfood Bosnalek” spray) did not result in the normalization of the investigated indicators of dermal energy metabolism. However, a positive effect of “Trichlomine” shampoo was found, which is associated with the presence of a plant antioxidant complex in the pharmacotherapeutic agent and its potentiating effect with minoxidil. Conclusions. It was found that the application of soft dosage forms of minoxidil (2 % emulsion-based cream and 1 % gel-based shampoo) exhibited pronounced growth-stimulating and follicle-protective activity in the model of experimental alopecia in white rats. At the same time, it was established that the cream with minoxidil 2 % for external use and the reference spray for external use “Pilfood Bosnalek” (Bosnalijek, Bosnia and Herzegovina) have a significant statistically comparable growth-stimulating effect. The strength of the growth-stimulating effect recorded from the use of the “Trichlomine” shampoo significantly exceeded the effect of the reference shampoo “Dove” (Unilever, Germany). It was found that both experimental medicinal forms of minoxidil (2 % cream and gel-like shampoo) had a follicle-protective effect by normalizing various links of dermis metabolism. The most pronounced effect in this regard was recorded in the shampoo “Trichlomine”, which contained not only minoxidil but also plant-antioxidant complexes. Application of 2 % cream with minoxidil, as well as reference drugs (spray “Pilfood Bosnalek” and shampoo “Dove”), did not have a reliable effect on the indicators of energy exchange of the dermis studied.

A Study on Changes in Hair Condition of Shampoo Containing Aroma Essential Oil

In this study, we investigated changes in hair after using an aroma shampoo containing aroma essential oils. The aroma essential oils included are Lavender, Juniper Berry, Cypress, Peppermint and Cedarwood. After the experimental group (15 persons) used the aroma shampoo for 4 weeks, the hair condition of the control group (15 persons) using the general shampoo for 4 weeks was compared and analyzed before and after. The hair was measured at 4 sites (left, right, parietal, and occipital regions) of the scalp, and changes in condition were measured with Aram Huvis' ASG (AI-Scalp Grader). As a result of the study, it was confirmed that the experimental group using aroma shampoo showed a significant increase in density and number in the occipital area, while the control group using general shampoo showed a significant decrease in number and thickness in the parietal area. By providing quantitative data that proves the hair improvement effect of aroma essential oil, it is expected that hair loss prevention and hair growth will be used as cosmetic ingredients.