Background: Low back pain is a common musculoskeletal disorder among healthcare specialty nurses. Operating room staff are particularly susceptible to experiencing low back pain due to the nature of their work. Objectives: We conducted a study to investigate the prevalence of low back pain and the factors associated with it. Methods: A cross-sectional study was conducted on 323 operating room staff from five hospitals affiliated with Ahvaz Jundishapur University of Medical Sciences. Data was collected using a questionnaire developed by Tavakol et al. This questionnaire included demographic information and assessed the prevalence and dimensions of low back pain. The data was analyzed using IBM SPSS v.26. Results: The results showed that the prevalence of low back pain (LBP) was 74.3%. In this study, 64.8% of the personnel were women, 63.5% were married, and 72.4% had a bachelor's degree. Additionally, 87% had no history of smoking. The average age was 33.83 ± 7.02 years. There was a significant relationship between the prevalence of LBP and gender (P = 0.044), marital status (P = 0.0001), BMI (P = 0.008), age (P = 0.0001), shift work (P = 0.001), work experience (P = 0.0001), and the average number of shifts (P = 0.037). Conclusions: We found a high prevalence of LBP among operating room personnel, which is related to both individual and occupational factors. Our recommendations for nursing managers include modifying work shifts, allocating less physically demanding tasks to senior personnel and females, and implementing educational programs that focus on improving ergonomics and reducing physical strain.