To determine the prevalence and clinical pattern of juvenile idiopathic arthritis (JIA) in a semi-urban area of Bangladesh. A cross-sectional study was carried out among 16270 children who were selected by using multistage sampling technique from a community of approximately 105986 children in the Narayanganj district, Bangladesh. Duration of the study was from November 2008 to December 2009. Examinations of the suspected JIA patients were done by the authors in the community as well as in the pediatric rheumatology follow-up clinic at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. The estimated point prevalence of JIA was 60.5 per 100000 children. In total 10 patients were identified among 16270 children. Girl:boy ratio was 2.3:1.0. The subgroup distribution showed oligoarticular JIA in the majority of patients (60%). Prevalence of JIA in this study in a semi-urban area of Bangladesh was consistent with established population-based studies in developed countries. Clinical pattern of JIA patients also had similarities with reports from Western countries.