Prevalence Of Cryptosporidium Research Articles

Molecular Epidemiology of Cryptosporidium parvum and Giardia lamblia in Different Water Bodies, Soil, and Vegetables in Pakistan.

There is a dearth of knowledge regarding transmission of Cryptosporidium (C) and Giardia (G) species through water and water-related sources in Pakistan. To this end, we conducted a study to evaluate the prevalence of these parasites in different water bodies, soil, and mixed raw vegetables in Pakistan. Researchers collected 200 samples from each reservoir including municipal water, sewage water, canal water, raw vegetables, soil of public parks, and soil of grazing areas. Researchers amplified the 18S ribosomal RNA gene of parasites using newly designed genus-specific primers through polymerase chain reaction testing. The sequencing analysis revealed that the obtained sequences belonged to C parvum and G lamblia. Phylogenetic clustering and sequence analysis of C parvum showed that the C parvum Pak1 (OM540369) and C parvum Pak2 (OM540370) as well as C parvum Pak3 (OM510450) and C parvum Pak6 (OM510445) were closely similar to each other. In the case of G lamblia, all the sequences appeared in the same clade. The epidemiological data showed lower prevalence of C parvum (11.5%) in all reservoirs, compared with G lamblia (20.5%). Among different reservoirs, prevalence of Cryptosporidium and Giardia was observed in sewage water (C = 13%, G = 26.5%), municipal water (C = 10%, G = 35%), canal water (C = 9.5%, G = 18.5%), raw vegetables (C = 5.5%, G = 8%), soil of public parks (C = 13%, G = 14%), and soil of grazing areas (C = 18.5%, G = 21.5%). Among targeted risk factors, poor hygienic conditions significantly affected the prevalence of parasites in sewage water (C = 20%, G = 41.2%), municipal water (C = 14.7%, G = 48.8.5%), raw vegetables (C = 11.3%, G = 15.1%), and soil of public parks (C = 19.5%, G = 21.9%). Similarly, contamination with sewage waste significantly affected (P < .05) the prevalence of these parasites in municipal water (C = 13.6%, G = 41.1%), canal water (C = 13.3%, G = 25%), raw vegetables (C = 12.1%, G = 15.1%), and soil of public parks (C = 23.3%, G = 15%). Results of this study illustrated a high risk of parasitic zoonosis through water bodies, soil, and vegetables.

Genotyping and subtyping of Cryptosporidium spp. and Giardia duodenalis isolates from two wild rodent species in Gansu Province, China

Cryptosporidium spp. and Giardia duodenalis are commonly detected intestinal protozoa species in humans and animals, contributing to global gastroenteritis spread. The present study examined the prevalence and zoonotic potential of Cryptosporidium spp. and G. duodenalis in Himalayan marmots and Alashan ground squirrels in China's Qinghai-Tibetan Plateau area (QTPA) for the first time. Four hundred ninety-eight intestinal content samples were collected from five counties of QTPA of Gansu province, China.All samples were examined for Cryptosporidium spp. and G. duodenalis by PCR amplification. The resultant data were statistically analyzed by chi-square, Fisher's test and Bonferroni correction using SPSS software 25. 0. Cryptosporidium positive samples were further subtyped through analysis of the 60-kDa glycoprotein (gp60) gene sequence. A total of 11 and 8 samples were positive for Cryptosporidium spp. and G. duodenalis, respectively. Prevalence of Cryptosporidium spp. and G. duodenalis were 2.5% (10/399) and 1.5% (6/399) in Himalayan marmots, 1.0% (1/99) and 2.0% (2/99) in Alashan ground squirrels, respectively. Sequence analysis confirmed the presence of C. rubeyi (n = 2), ground squirrel genotype II (n = 7), chipmunk genotype V (n = 1) and horse genotype (n = 1). The horse genotype was further subtyped as novel subtype VIbA10. G. duodenalis zoonotic assemblages A (n = 1), B (n = 6), E (n = 1) were identified in the present study. This is the first study to identify Cryptosporidium spp. and G. duodenalis in Himalayan marmots and Alashan ground squirrels, suggesting the potential zoonotic transmission of the two pathogens in QTPA.

Decline in Cryptosporidium Infection in Free-Ranging Rhesus Monkeys in a Park After Public Health Interventions.

Nonhuman primates (NHPs) are considered an important source of parasitic zoonoses. A study in 2010 revealed high prevalence of Cryptosporidium spp. in free-ranging rhesus monkeys (Macaca mulatta) in a public park in Guiyang, southwestern China, which called for the control of disease in animals and long-term epidemiological tracking of Cryptosporidium spp. After the initiation of a series of public health interventions, we collected 2,402 fecal samples from monkeys and 123 water samples from lakes in the park on six occasions during 2013-2019. They were analyzed and genotyped for Cryptosporidium spp. using PCR and sequence analyses of the small subunit rRNA gene. The C. hominis and C. parvum identified were further subtyped by sequence analysis of the 60 kDa glycoprotein gene. Compared with the high prevalence of Cryptosporidium spp. in fecal samples (10.9% or 45/411) and water samples (47.8% or 11/23) in 2010, only 18 (0.7%) fecal samples and 3 (2.4%) water samples collected in the present study were positive for Cryptosporidium spp., including C. hominis (n = 9) and C. parvum (n = 12). The former belonged to the NHP-adapted IfA17G2R3 subtype, while the latter mostly belonged to rodent-adapted IIpA9. Therefore, the detection rate and genetic diversity of Cryptosporidium spp. during this study period were much lower than those before the public health interventions, and there was a switch from common occurrence of anthroponotic C. hominis subtypes to sporadic occurrence of NHP-adapted C. hominis and rodent-adapted C. parvum subtypes.

Prevalence of Cryptosporı̇dı̇um spp. in Dogs in The Aegean Region

Cryptosporidium spp. is a protozoal parasite that can cause significant gastrointestinal diseases in humans and animals. Those parasites cause infection, especially in young and immunosuppressed animals in many mammals, poultry, reptiles, and humans, Cryptosporidium spp. Localised in the digestive system, epithelial cells threaten human and animal health with their zoonotic properties. The study's animal material consisted of 200 dogs of different breeds, ages and genders, including healthy (n=50) and diarrhoea (n=150). Stool samples of the dogs used in the study were stained with modified Ziehl-Neelsen technique and examined under a microscope. It was concluded that the prevalence of Cryptosporidium spp. was 15.5% in faecal samples taken from dogs and that the prevalence of Cryptosporidium spp. was 14% and 16%, respectively, in healthy and diarrheal dogs, and it was concluded that it could be used as a reference to future studies in dogs.

Prevalence of Protozoan Parasites Cryptosporidium sp. and Callimastix equi in the Edible Oyster Saccostrea cucullata and Its Relationship with Selected Water Quality Parameters in Sundarbans Region of West Bengal, India.

Prevalence of protozoan parasites Cryptosporidium sp. and Callimastix equi in the edible oyster resources Saccostrea cucullata, and its relationship with selected water quality parameters such as temperature, pH and salinity in Sundarbans region of West Bengal, India, have been assessed and discussed elaborately. Edible oysters S. cucullata were collected randomly from the selected study site Frasergunj of Sundarbans region and protozoan parasites such as Cryptosporidium sp. and C. equi were isolated, stained and determined its prevalence. Relationship of water temperature, pH and salinity with the prevalence of protozoan parasite had been assessed and analyzed. Prevalence of infection of Cryptosporidium sp. is much more than the C. equi found in S. cucullata. The infections of the parasites are higher during monsoon then pre-monsoon season. Significant negative correlation (P < 0.05) occurs between pH of water and prevalence of Cryptosporidium sp. and C. equi in the edible oyster S. cucullata collected from Frasergunj of Sundarbans region, South 24 Parganas of West Bengal. The C. equi parasite follows a seasonal pattern of infection varying significantly with salinity (P < 0.01) of the water in the edible oyster, S. cucullata collected from Frasergunj of South 24 Parganas of West Bengal. These results indicate that the Cryptosporidium sp. and C. equi contamination is accompanying with public health risks if edible oysters are consumed uncooked of undercooked. Extremes and/or rapid fluctuations of important environmental factors such as water temperature, salinity, pH can seriously compromise oyster health and outcome of aquaculture.

Dairy Farm Management Practices as Risk Factors Linked to Cryptosporidium spp. Infection in Dairy Calves

Background: Cryptosporidiosis is the disease of zoonotic importance and is considered to be third major cause of diarrhoeal disease of calves worldwide causing significant economic losses. The present study aimed to link Cryptosporidium spp. infections in dairy calves with the management practices as a major risk factor. Methods: Total 401 faecal samples from dairy calves were collected from eight large (100-1000 animals) and eight medium size (30-100 animals) dairy farms located in Jabalpur (Madhya Pradesh, India). Cryptosporidium oocysts were identified in faeces by modified Ziehl-Neelsen staining (mZN) and formal ether concentration techniques. Result: The prevalence of Cryptosporidium spp. was 28.7% in dairy calves. A higher prevalence and risk of cryptosporidiosis was associated with large farms (24.9% vs. 3.74%, OR=1.96), intensive farming system (27.7% vs. 0.99%, OR=1.86), drinking ground water (27.7% vs. 0.99%, OR=1.86), dirty farms (16.7% vs. 11.6%, OR=1.90), presence of other animals at farm (15.9% vs. 12.7%, OR=1.36), absence of deworming (14.7% vs.13.9%, OR=5.09) and diarrhoea treatment practice (16.2% vs. 12.5%, OR=1.07). Multi-animal calving area, dirty calving pen and cemented floor were also identified as risk factors. Practices like feeding colostrum within one hour of birth, @ 10% b.wt./day, weaning the calves within 24 hrs and using detergent to wash feeding utensils decreased the risk of infection from 1.4 to 2 times. Calves kept confined with their dam had greater prevalence and risk of infection (17.2% vs. 11.5%, OR=5.20). The findings highlight that proactive managemental practices are beneficial for decreasing the environmental contamination and improving calf health.

Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission

BackgroundCryptosporidium is one of the most prevalent parasites infecting both birds and mammals. To examine the prevalence of Cryptosporidium species and evaluate the public health significance of domestic chickens in Guangdong Province, southern China, we analyzed 1001 fecal samples from 43 intensive broiler chicken farms across six distinct geographical regions.MethodsIndividual DNA samples were subjected to nested PCR-based amplification and sequencing of the small subunit of the nuclear ribosomal RNA gene (SSU rRNA). Analysis of the 60 kDa glycoprotein gene (gp60) was performed to characterize the subtypes of C. meleagridis.ResultsThe overall prevalence of Cryptosporidium was 13.2% (95% CI 11.1–15.3) (24 of 43 farms), with C. meleagridis (7.8%), C. baileyi (4.8%) and mixed infections (0.6%). Using the gp60 gene, three subtype families, IIIb, IIIe and IIIg, were identified, including six subtypes: one novel (IIIgA25G3R1a) and five previously reported (IIIbA23G1R1c, IIIbA24G1R1, IIIbA21G1R1a, IIIeA17G2R1 and IIIeA26G2R1). Within these subtypes, five known subtypes were genetically identical to those identified in humans.ConclusionsThis is the first report of C. meleagridis in chickens from Guangdong. The frequent occurrence of C. meleagridis in domestic chickens and the common C. meleagridis subtypes identified in both humans and chickens is of public health significance. Our study indicates that broiler chickens represent a potential zoonotic risk for the transmission of Cryptosporidium in this region.Graphical

Prevalence and genotypes of Cryptosporidium in livestock in Hualien Country, Eastern Taiwan.

Cryptosporidium spp. is a group of protozoans that cause diarrheal disease in both humans and animals. In Taiwan, very little information is available about the epidemiology of Cryptosporidium spp. in domesticated animals, especially in Eastern Taiwan where agriculture is one of the main industries. Therefore, this study aimed to investigate the occurrence of Cryptosporidium spp. in livestock in Hualien Country of Eastern Taiwan and identify their genotypes. Excrements from dogs (n=81), cattle (n=156), and pigs (n=142) were randomly collected from different pastures or farm in Hualien Country. Microscopic examination and nested PCR were performed on all samples and both showed identical results, with 4.94% (4/81) of dogs, 24.36% (38/156) of cattle, and 16.20% (23/142) of pigs being infected with Cryptosporidium species. Positive samples were then sequenced and analyzed. DNA sequencing revealed that all four positive samples isolated from dogs were Cryptosporidium canis (C. canis); 38 positive samples from cattle were identified as C. bovis (8/38), C. canis (1/38), C. ryanae (4/38), and C. scrofarum (25/38); and 22 positive samples isolated from pigs were identified as C. scrofarum while one was identified as C. suis. In addition, the infective rates of animals from indoor farms (57.14% of all positive samples) are much higher than the rates from pastures. This study provided evidence of the occurrence of Cryptosporidium spp. in Hualien country, and farming conditions largely affect their infection rates. Therefore, precautions should be made to control Cryptosporidium spp. transmission.

Prevalence of Cryptosporidium infection in slaughtered cattle at Lafenwa Municipal Abattoir, Ogun State

The prevalence of Cryptosporidium in slaughtered cattle at a municipal abattoir in Ogun State was determined by acid-fast staining of stools. Using formalin ethyl acetate sedimentation followed by modified kinyoun’s acid fast staining of 280 faecal samples collected, 105 (37.5%) were positive for Cryptosporidium oocysts. Infection was higher in females and nondiarrhoeic cattle than males and diarrhoeic animals respectively. However, there were no statistical differences (p&gt;0.05) between the sexes and stool consistencies in Cryptosporidium infection. The highest infection rate, 51.2%, was recorded in the Red Bororo breed while the lowest rate, 17.0%, was recorded in the Sokoto Gudali breed. This study showed that cattle slaughtered at the abattoir were mostly infected with Cryptosporidium which may have serious economic, veterinary, and public health implications to beef consumers, abattoir, and farm workers within the state.

First Epidemiological Report on the Prevalence and Associated Risk Factors of Cryptosporidium spp. in Farmed Marine and Wild Freshwater Fish in Central and Eastern of Algeria.

The present study aimed to estimate the prevalence and molecular characterization of Cryptosporidium spp. in six different fish species both from marine and freshwater environments. During a period of 2 years (2018-2020), a total of 415 fecal samples and 565 intestinal scrapings were collected in seven provinces from the central and eastern Algeria. From those, 860 fish belonged to six different species, two of which are cultured marine and four are wild freshwater fish. All samples were screened for Cryptosporidium spp. presence using molecular techniques. Nested PCR approach was performed to amplify partial sequences of the small subunit ribosomal RNA (SSU rRNA) and 60-kDa glycoprotein (GP60) genes for Cryptosporidium genotyping and subtyping. Detailed statistical analysis was performed to assess the prevalence variation of Cryptosporidium infection according to different risk factors. Nested PCR analysis of SSU gene revealed 173 Cryptosporidium positive fish, giving an overall prevalence of 20.11% (17.5-23.0). Cryptosporidium spp. was detected in 8.93% (42/470) of culturedmarine fish and 33.58% (131/390) of wild freshwater fish. Overall, the prevalence was affected by all studied risk factors, except the gender. Molecular characterization and subtyping of Cryptosporidium isolates showed occurrence of IIaA16G2R1 and IIaA17G2R1 subtypes of C. parvum in the fish species Sparus aurata. The present study provides the first epidemiological data on the prevalence and associated risk factors of Cryptosporidium spp. in farmed marine and wild freshwater fish and the first molecular data on the occurrence of zoonotic C. parvum in fish from North Africa (Algeria).

Sponges (Porifera: Spongillida) as ecological indicators for parasitic protozoans Cryptosporidium and Giardia infective stages in freshwater ecosystems

Sponges filter large volumes of water by actively pumping it through their bodies to acquire nutrients, while Cryptosporidium and Giardia are aquatic ecosystems' primary faecal parasitic pollutants. Five aquatic matrices each of surface water (SW), water sediments (WS), substrate-associated biofilms (SAB), as well as five sponges collected from Lake Buhi were investigated for the presence of parasitic protozoan (oo) cysts. Modified Kinyoun's acid-fast stain (MK) was used to detect Cryptosporidium, while immunofluorescence (IFT) staining was employed to detect both Cryptosporidium and Giardia. Scanning Electron Microscopy (SEM) was used to visualize the skeletal sponge structures and simulated contamination with C. parvum oocysts. Aquatic matrices of WS and SAB demonstrated a 20% (3/15) positivity for Cryptosporidium spp. oocysts with MK and IFT. Overall, the study site prevalence of Cryptosporidium spp. oocysts in aquatic matrices were 60% (3/5). Freshwater sponges demonstrated 100% (5/5) Cryptosporidium and 20% (1/5) Giardia (oo) cysts positivity through IFT. Sponges were ascribed to Radiospongilla cfr. philippinensis (Annandale, 1909) based on diagnostic morphotraits of the siliceous skeleton, i.e., megascleres and gemmuloscleres identified under light microscopy (LM) and SEM. Sponges spiked with Cryptosporidium parvum oocysts simulated parasitic protozoan contamination and demonstrated that oocysts could fit through and be trapped within the sponge aquiferous canals. The presence of Cryptosporidium spp. oocysts in aquatic matrices and Cryptosporidium and Giardia spp. (oo) cysts in Radiospongilla cfr. philippinensis demonstrated the contamination of Porifera with infective stages of both parasites. This study submits a promising perspective on the role of freshwater sponges as ecological indicators, i.e. reservoirs and potential biomonitors for the contamination of infective stages of waterborne parasitic protozoans in aquatic ecosystems.

Genetic diversity of Cryptosporidium spp. in non-human primates in rural andurban areas of Ethiopia.

Non-Human Primates (NHPs) harbor Cryptosporidium genotypes that can infect humans and vice versa. NHPs Chlorocebus aethiops and Colobus guereza and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of Cryptosporidium. This cross-sectional study examined the molecular prevalence and subtypes of Cryptosporidium spp. from 185 fecal samples of Chlorocebus aethiops and Colobus guereza in rural and urban areas in Ethiopia. Samples were tested for Cryptosporidium infection using nested polymerase chain reaction (PCR), and subtypes were determined by sequencing a fragment of the 60-kDa glycoprotein gene (gp60). Of the 185 samples, fifty-one (27.56%) tested positive for Cryptosporidium infection. The species detected were C. parvum (n = 34), C. hominis (n = 12), and C. cuniculus (n = 3). Mixed infection with C. parvum and C. hominis were detected in 2 samples. Four C. hominis family subtypes (Ia, Ib, Id, and Ie) and one C. parvum family subtype (IIa) were identified. C. hominis IaA20 (n = 7) and C. parvum IIaA17G1R1 (n = 6) were the most prevalent subtypes detected. These results confirm that Chlorocebus aethiops and Colobus guereza can be infected with diverse C. parvum and C. hominis subtypes that can also potentially infect humans. Additional studies could help to understand the role of NHPs in the zoonotic transmission of Cryptosporidium in Ethiopia.

Prevalence of Cryptosporidium spp. in Horses from communities of the Mapuche native people, Araucanía Region, Chile.

There are no studies of potential zoonotic diseases in Mapuche communities' horses. The objective of this study was to determine the prevalence of Cryptosporidium in horses of the Mapuche communities. This was a cross-sectional study. Faecal samples from 100 randomly selected horses (n=100) were taken from rural Mapuche communities from four municipalities from the Araucanía Region. These samples were processed with the modified Ziehl-Neelsen staining technique and grouped by sex, age and municipality. The general prevalence of Cryptosporidium spp. was 67.0% (n=67). The prevalence was 51.0% (n=51) in males and 49.0% (n=49) in females, and there is no gender association to the presentation of Cryptosporidium spp. The prevalence by municipality was 60.0%, 80.0%, 64.0% and 64.0% in Curarrehue, Lonquimay, Padre las Casas and Teodoro Schmidt, respectively. The above shows no significant association between the sector and the presence of Cryptosporidium spp. The prevalence by age was 95.4% of horses tested positive for Cryptosporidium between birth and 6years of age. About 27.3% of horses were tested positive in the age group between 7 and 10years. There was no presence of Cryptosporidium spp. in the age group older than 11years, showing a significant relationship between the age of the animal and the presence of Cryptosporidium (P˂0.05). The sample analysis did not specifically identify the type of Cryptosporidium, and it was not possible to evaluate the zoonotic risk in the Mapuche communities. Cryptosporidium spp. is present in working horses in Mapuche communities, with a 67.7% general prevalence, and there is a significant association between this parasite and the age of the horses, being higher in the age group between 0 and 6years, with a prevalence of 95.4%. There may be a potential zoonotic risk in the Mapuche communities.

Survey of zoonotic parasites and bacteria in faeces of Canada geese (Branta canadensis) in North-Central Oklahoma.

BackgroundAs a population of non‐migratory Canada geese (Branta canadensis) has been growing in residential and recreational areas, public concerns on potential acquisition of zoonotic pathogens from Canada geese and their faecal deposits have been increasing.ObjectivesThe main study objective was to evaluate the prevalence of zoonotic microorganisms, Campylobacter spp., Cryptosporidium spp., Giardia spp. and Salmonella spp. and antimicrobial resistant Escherichia coli in faeces of Canada geese residing in North‐Central Oklahoma, United States.MethodsA total of 204 faecal samples were collected from 11 locations in North‐Central Oklahoma, where public recreational areas such as lakes and ponds were located, and Canada geese were commonly inhabited. Faecal samples were examined by a centrifugal flotation to evaluate the prevalence of Cryptosporidium spp. and Giardia spp. infections. A total of 180 faecal samples were grouped into 36 pooled samples and cultured using standard culture methods to detect the prevalence of Campylobacter spp. and Salmonella spp. infections. The antimicrobial resistance profile was determined on 32 E. coli isolates recovered from the 36 sample pools, using the Kirby Bauer Disk Diffusion method.ResultsThe targeted zoonotic pathogens were not identified by the faecal examinations performed. Of the 32 E. coli isolates, 17 isolates (53.1%) demonstrated resistance to ≥1 antimicrobial agent.ConclusionsTargeted zoonotic pathogens were not detected among the examined resident Canada geese in North‐Central Oklahoma. The findings of multiple‐antimicrobial resistant E. coli infections are potentially a public health concern although the prevalence was low in this study. Further, larger scale surveys are recommended.

Molecular prevalence and subtyping of Cryptosporidium spp. in fecal samples collected from stray cats in \u0130zmir, Turkey

BackgroundCryptosporidium spp. are obligate intracellular apicomplexan parasites transmitted to humans and other animals by contaminated water, food, or direct contact. They mainly cause gastrointestinal symptoms, although subclinical infections are also common. Cats are primarily infected by host-adapted Cryptosporidium felis while C. parvum and C. muris have also been detected in some cases. In this study, the molecular prevalence of Cryptosporidium spp. was investigated by screening 399 fecal samples collected from stray cats using nested PCR targeting the 18S rRNA gene for the first time in Turkey. Additionally, Cryptosporidium PCR-positive samples were genotyped by nested PCR- restriction fragment length polymorphism (RFLP), and subsequently, amplicons of 18S SSU rRNA were sequenced. They were further subtyped by amplification and sequencing of the gp60 gene.ResultsAmong fecal samples screened, 12 of them (3%) were found to be Cryptosporidium-positive, and according to RFLP and sequencing of 18S rRNA gene, all positive samples were identified as C. felis. Subtyping analyses at the gp60 gene showed that C. felis isolates belonged to the XIXa subtype family, which are closely related to human subtypes of the parasite.ConclusionsThe results of this study are important in terms of indicating the potential role of stray cats for transmission of Cryptosporidium spp. to humans or other animals. Also, the presence of XIXa, which is the dominant subtype family of C. felis in cats and humans was shown for the first time in stray cats of İzmir, Turkey.

Prevalence and molecular characterization of Cryptosporidium sp. in ruminant livestock in South Sinai, Egypt

Prevalence and molecular characterization of Cryptosporidium sp. in ruminant livestock in South Sinai, Egypt

High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms.

Cryptosporidium parvum is one of the major causes of neonatal calf diarrhoea resulting in reduced farm productivity and compromised animal welfare worldwide. Livestock act as a major reservoir of this parasite, which can be transmitted to humans directly and/or indirectly, posing a public health risk. Research reports on the prevalence of Cryptosporidium in ruminants from east Mediterranean countries, including Cyprus, are limited. This study is the first to explore the occurrence of Cryptosporidium spp. in cattle up to 24 months old on the island of Cyprus. A total of 242 faecal samples were collected from 10 dairy cattle farms in Cyprus, all of which were screened for Cryptosporidium spp. using nested-PCR amplification targeting the small subunit of the ribosomal RNA (18S rRNA) gene. The 60 kDa glycoprotein (gp60) gene was also sequenced for the samples identified as Cryptosporidium parvum-positive to determine the subtypes present. The occurrence of Cryptosporidium was 43.8% (106/242) with at least one positive isolate in each farm sampled. Cryptosporidium bovis, Cryptosporidium ryanae and C. parvum were the only species identified, while the prevalence per farm ranged from 20–64%. Amongst these, the latter was the predominant species, representing 51.8% of all positive samples, followed by C. bovis (21.7%) and C. ryanae (31.1%). Five C. parvum subtypes were identified, four of which are zoonotic—IIaA14G1R1, IIaA15G1R1, IIaA15G2R1 and IIaA18G2R1. IIaA14G1R1 was the most abundant, representing 48.2% of all C. parvum positive samples, and was also the most widespread. This is the first report of zoonotic subtypes of C. parvum circulating in Cyprus. These results highlight the need for further research into the parasite focusing on its diversity, prevalence, host range and transmission dynamics on the island.

The infection and molecular characterization of Cryptosporidium spp. in diarrheic pigs in southern China

Cryptosporidium spp. is recognized as an opportunistic zoonotic parasite that infects humans, wild and domestic animals, and is also a major cause of diarrhea-related disease in immunocompromised individuals, considered a global public health concern. Pig is considered as one of the reservoir hosts of Cryptosporidium spp. can transmit cryptosporidiosis to humans and other animals. However, limited studies on the distribution of Cryptosporidium spp. in diarrheic pigs have been published. Objective of the current study was to investigate the infection and species/genotypes of Cryptosporidium spp. from feces of diarrheic pigs in southern China. A total of 1254 fresh fecal samples were collected from 37 intensive pig farms in Jiangxi, Hunan and Fujian provinces, and were screened for Cryptosporidium spp. infection using a nested PCR assay targeted the small subunit rRNA (SSU rRNA) genes. The overall infection rate of Cryptosporidium spp. was 4.5% (57/1254), including 5.5% (17/307) in suckling piglets, 2.7% (8/299) in weaned piglets, 7.4% (7/95) in fattening pigs and 4.5% (25/553) in sows, respectively. In addition, two human-pathogenic species Cryptosporidium scrofarum (80.7%, 46/57) and Cryptosporidium suis (19.3%, 11/57) were identified. C. scrofarum and C. suis were observed in pigs tested in all age groups. Interestingly, a high colonization incidence of C. scrofarum (16/57) was observed in suckling piglets. This study revealed the prevalence and species of Cryptosporidium spp. in diarrheic pigs in three provinces of southern China, which suggested that diarrhea maybe not a direct factor affecting the prevalence of Cryptosporidium spp. in pigs. More prevention and control of this parasite in pigs should receive greater attention from farmers in investigated provinces.

Burdens of Ascaris spp. and Cryptosporidium spp. parasites in farm pigs in Ghana.

BackgroundWorldwide, intestinal parasites significantly affect the health and production of pigs.ObjectiveThis study assessed the prevalence of Ascaris and Cryptosporidium infection in pigs in the Ejisu‐Juaben Municipality of Ghana.MethodFaecal samples from two hundred (200) pigs on four different farms (labelled A, B, C, D) were processed using the Kinyoun modified Ziehl‐Neelsen method for Cryptosporidium and the Formol‐ether sedimentation method for Ascaris and microscopically examined to identify parasites to the genus level.ResultsThe prevalence of Ascaris and Cryptosporidium in the pigs was 76% and 77%, respectively. The weaners had the highest Ascaris prevalence (96.15%) with the piglets recording the least (59.25%). On the other hand, the piglets had the highest prevalence (88.89%) for Cryptosporidium with the boars, sows and weaners recording 75.86%, 75.42% and 73.08% respectively. The prevalence of Ascaris was high in farm D (78.57%) while Cryptosporidium was highest in farm C (86.11%). Generally, there was a significant difference (p = 0.044) in the mean distribution of Cryptosporidium in the pigs.ConclusionThe high burden of Ascaris and Cryptosporidium infections in the pigs suggest the need to adopt and implement effective control measures.

Prevalence, risk factor and diversity of Cryptosporidium in cattle in Latvia.

The epidemiology of Cryptosporidium spp. in Latvia was investigated by testing fecal samples from 926 animals aged from one day to 24years for the presence of Cryptosporidium spp. oocysts. The samples were collected from 87 cattle farms and from four slaughterhouses, and analyzed by conventional and fluorescent microscopy, followed by Cryptosporidium species and C. parvum subtype differentiation. Moreover, using a questionnaire, we surveyed factors that could be relevant as risk factors of Cryptosporidium spp. infection on the farms. Cryptosporidium spp. were shed by 33.8% of the investigated cattle and at least one shedding animal was found on 77.8% of the farms. In the present study, all four Cryptosporidium species reported to commonly infect cattle and two additional Cryptosporidium species (C. scrofarum and C. ubiquitum) were identified. In addition, mix infections of C. parvum/C. bovis, C. bovis/C. ryanae, C. parvum/C. ryanae, C. parvum/C. andersoni and C. bovis/C. andersoni were observed. C. parvum and C. bovis was mostly prevalent in young animals (0-3months old) and in addition, diarrhea associated with C. parvum infection was observed only in very young animals. Cryptosporidium andersoni and C. ryanae in age group 0-3months was observed in low prevalence, while a higher proportion of animals with diarrhea associated with C. andersoni infection was observed in very young animals and with C. ryanae in animals age group 4-24months. Eight previously described C. parvum subtypes were observed. The majority of the subtypes were in the IIa subtype family, while one subtype was identified from the IId subtype family. The most common subtype was IIaA15G2R1, which was found in 34.2% of the C. parvum successfully subtyped samples. The probability of Cryptosporidium spp. associated diarrhea in cattle decreased significantly with the age of the animals and a prolonged period during which calves were fed with milk.