The developing sarcomeres in the lateral musculature of 60-somite trout embryos have been examined with special reference to the ultrastructure and sequence of events accompanying sarcomere formation. Myogenesis begins at the first somites in the head region and progresses towards the tail of the embryo. The tail somites are composed of undifferentiated presumptive myoblasts, myoblasts and mesenchyme cells. The very tip of the tail contains a mass of undifferentiated cells. Myofibrils with fully developed sarcomeres and well-organized sarcoplasmic reticulum are present in the midbody somites. Microtubules are found in muscle cells throughout the period preceding the sarcomere assembly. They may represent a cytoskeletal network which contributes directly to the shape of myoblasts. Thick and thin filaments appear mostly near the periphery of the cell. In successive stages of the sarcomere development "Z-bodies" appear, which then coalesce to form Z-bands. The assembly of the thick and thin filaments into sarcomeres seems to occur at that stage of myogenesis when the "Z-bodies" develop a certain amount of bonding sites for thin filaments, which interact with thick filaments to form A-bands and I-bands.