Levels of residual prestress forces are key parameters when assessing the structural behaviour of existing prestressed concrete bridges. However, these parameters are often unknown and not easy to determine. To explore them, two existing non-destructive and destructive approaches have been further developed for practical application and demonstrated on a multi-span continuous girder bridge. The evaluation of the prestress forces was part of an extensive experimental programme aimed to calibrate and develop assessment methods. Due to the pursuit of practical applications for existing bridges, the main focus was on non-destructive methodology, combining experimental data and finite element modelling to obtain the residual prestress forces. Assuming that the initial prestress force corresponded to 85% of the characteristic 0.2% proof strength of the reinforcing steel, estimated losses in investigated sections ranged between 5 and 70%. However, determined residual prestress forces were generally higher than theoretically based estimates accounting for friction and time-dependent losses in the prestressing system. In addition to describing in detail the methods for prestress evaluation, this paper presents suggestions for improvements and further studies, based on experiences from the field tests.