Background: Professional nurses are saddled with a unique role in patient's skincare among hospitalized patients. Inadequate nurses’ knowledge of pressure ulcer risk assessment (PURA) and prevention as a major contributory factor to development of pressure ulcer among “at risk” patients has been identified by previous studies. Aim: To evaluate the impact of an educational intervention on nurses’ knowledge of pressure ulcer risk assessment and prevention at a tertiary hospital, Ibadan, Nigeria. Methods: A pilot study with sample size of 26 nurses selected through simple random sampling technique from selected nursing units of the facility. Data collection was done through a structured questionnaire. Hypotheses were tested with inferential statistics using t-test. Results were presented in descriptive statistics and charts. Level of significance was p-value <0.05. Results: Almost all participants’ knowledge mean score of PURA increased from 4.92 ±1.26 at pre-test to 14.00 ± 0.71 at post-test (p<0.001). Also, knowledge mean score of pressure ulcer prevention improved among intervention group (14.00 ± 0.707) post educational intervention as compared to their counterparts in control group (11.85±1.41). Educational intervention was statistically significant with participants’ knowledge of PURA and prevention (p<0.001). Conclusion: The results of this study confirmed that educational intervention was impactful positively on nurses’ knowledge regarding PURA and prevention strategies at UCH, Ibadan, Nigeria. Thus, a structured educational program on the subject matter is needed to promote quality nursing care delivery according to international best practices among Nigerian nurses.