Recently, flexible wearable electronics for human running posture monitoring and human energy harvesting have attracted widespread attention. Hence, we design a mixed type conductive hydrogel based on polyvinyl alcohol, cotton paper, graphite oxide, and MXene, named PCGM hydrogel. Furthermore, the PCGM hydrogel can act as the PCGM-based strain sensor and triboelectric nanogenerator (P-TENG) for running posture monitoring and mechanical energy harvesting. The PCGM-based strain sensor has two sensing linear regions: The pressure sensitivity is 0.0164 kPa−1 in the low pressure region (0–16 kPa), whereas it is 0.002 86 kPa−1 in the high pressure region (16–120 kPa). To achieve comprehensive health monitoring of runners, the PCGM-based strain sensors can be installed on human joints and facial skin to monitor human posture and facial expressions. The PCGM hydrogel can be combined with a polytetrafluoroethylene film to form a P-TENG device for mechanical energy harvesting. The P-TENG maximum output power can reach 135 µW with a 30 MΩ load. The short-circuit current (Isc), open-circuit voltage (Voc), and transfer charge (Qsc) of P-TENG can reach 10.36 µA, 229.85 V, and 49.24 nC, respectively. This research provides an effective approach for human-running motion monitoring by using multifunctional flexible devices.