Preservation Of Food Products Research Articles

Application of water soluble chitosan for vannamei shrimp (Litopenaeus vannamei) preservation at chilling temperature

Active biomolecules such as chitosan and its derivatives have a role in food products preservation due to its antimicrobial properties. The aim of this study is to determine the ability of water soluble chitosan to extend shelf life of peeled and deveined shrimp stored at the chilling temperature. This study used completely randomized design using three treatments (control, 2% chitosan solution, and 2% water soluble chitosan solution) with six replications. Samples were tested for aerobic plate count, pH, total volatile base nitrogen, and sensory (appearance, odor, and texture). Experiments on water soluble chitosan indicated number of microbes was still within the safe limit until the 6th day. This could be identified from the number of microbes which still could be accepted by consumers (< 5 x 104 colonies/gram) at the 6th day, while in the control treatment the microbial amount within the safe limit until the 3rd day. Total Volatile Base Nitrogen of water soluble chitosan treatment was 3.69 mgN/100 grams. The pH value of shrimp treated with water soluble chitosan was 6.8, while in the organoleptic test, water soluble chitosan treatment had significant difference from control (P <0.05). This research conclude that water soluble chitosan have properties that can preserve food products specially vanamei shrimp.

Development of biodegradable coatings by the incorporation of essential oils derived from food waste: A new sustainable packaging approach

AbstractPackaging serves as a pivotal component in addressing food losses by extending the shelf life and preserving the quality of both raw and processed food products. Nevertheless, conventional plastic packaging has substantial environmental drawbacks due to its non‐biodegradable nature and the inclusion of potentially harmful chemical components. As a result, there is a global shift toward biodegradable packaging obtained from natural sources as a more environmentally friendly and sustainable alternative. In this regard, biodegradable coatings made up of natural sources are very efficient for food packaging purposes because of various significant properties. Moreover, huge amounts of food waste are produced globally, leading to environmental pollution. Utilization of this waste is crucial, with one significant application being the extraction of essential oils (EOs). These oils are incorporated into different coatings materials to enhance their functional properties for better preservation of food products. So, this review covers the potential of EOs derived from food waste materials as bioactive compounds incorporated in biodegradable coatings for enhancing the storage quality and shelf life of food products. The biodegradable coatings enriched with these EOs thereby reduce microbial spoilage, stop moisture loss and act as a barrier against gases that ultimately enhance the product's shelf life. These coatings with EOs also reduce weight loss and stabilize other parameters such as pH, colour, acidity and firmness of food commodities. Different packaging systems using waste‐derived EOs have been developed to preserve both plant and animal‐origin foods such as fruits, meat, fish, shrimp, mushrooms and cheese. Developing biodegradable packaging from natural polymers along with the incorporation of EOs from food waste is an innovative way of food preservation and waste management.

Effect of alkaline pH values on physical and functional properties of edible films made from calcium caseinates

Films are an important tool in food packaging, delivery, and preservation of food products. Milk based films have shown the potential to be renewable, edible, and provide a lower cost option to replace plastic films, but there is still a need to find formulations that have superior physical properties and can be made with cheaper materials. Research has suggested that pH modification of the film solution into alkaline conditions could improve film properties. Calcium caseinate based films were made with increased pH to study their physical qualities. Film strength for CaCN films reached a relative maximum at pH 8. CaCN films also showed increasing strength and decreasing elongation with aging. Increasing pH above 10 yielded the lowest oxygen permeabilities for both recipes. Calcium caseinate films with modified pH have many beneficial properties, performing better than previously reported milk-based films in a number of categories and are promising candidates for food applications.

The Extraction and Impact of Essential Oils on Bioactive Films and Food Preservation, with Emphasis on Antioxidant and Antibacterial Activities-A Review.

Essential oils, consisting of volatile compounds, are derived from various plant parts and possess antibacterial and antioxidant properties. Certain essential oils are utilized for medicinal purposes and can serve as natural preservatives in food products, replacing synthetic ones. This review describes how essential oils can promote the performance of bioactive films and preserve food through their antioxidant and antibacterial properties. Further, this article emphasizes the antibacterial efficacy of essential oil composite films for food preservation and analyzes their manufacturing processes. These films could be an attractive delivery strategy for improving phenolic stability in foods and the shelf-life of consumable food items. Moreover, this article presents an overview of current knowledge of the extraction of essential oils, their effects on bioactive films and food preservation, as well as the benefits and drawbacks of using them to preserve food products.

.Cinnamaldehyde-enriched Pickering emulsions stabilized by modified cellulose I and II nanocrystals recycled from maple leaves for shrimp preservation

The utilization of biomass waste has attracted much interest, but such attention hasn't been paid to the abundant fallen maple leaves in Canada. Herein, we aim to obtain cellulose nanocrystals (CNCs) from maple leaves and explore their potential applications as sustainable stabilizers of Pickering emulsions for the preservation of food products with complicated structures. The results reveal that two types of CNCs were extracted from maple leaves at different alkaline conditions. Octenyl succinic anhydride was selected to modify rod-like CNCs, and the CNC-stabilized oil-in-water Pickering emulsions showed excellent stability. Cinnamaldehyde, a model antibacterial compound, was incorporated in the Pickering emulsions, which exhibited the improved storage stability and sustained antibacterial capacity towards both Gram-positive and Gram-negative bacteria. Shrimp was chosen as an example that has complicated surface structure and is hard to disinfect, and the CNC-stabilized Pickering emulsions could be easily sprayed on the surface of shrimp to inhibit the proliferation of bacteria and inactivate the psychrophilic bacteria responsible for shrimp spoilage at refrigerated condition, so as to preserve the quality of shrimp. Therefore, the current work suggests the possibility to utilize fallen maple leaves as a promising source of CNCs and the applications of CNC-stabilized Pickering emulsions in seafood preservation.

Spices as Sustainable Food Preservatives: A Comprehensive Review of Their Antimicrobial Potential.

Throughout history, spices have been employed for their pharmaceutical attributes and as a culinary enhancement. The food industry widely employs artificial preservatives to retard the deterioration induced by microbial proliferation, enzymatic processes, and oxidative reactions. Nevertheless, the utilization of these synthetic preservatives in food products has given rise to significant apprehension among consumers, primarily stemming from the potential health risks that they pose. These risks encompass a spectrum of adverse effects, including but not limited to gastrointestinal disorders, the disruption of gut microbiota, allergic reactions, respiratory complications, and concerns regarding their carcinogenic properties. Consequently, consumers are displaying an increasing reluctance to purchase preserved food items that contain such additives. Spices, known for their antimicrobial value, are investigated for their potential as food preservatives. The review assesses 25 spice types for their inherent antimicrobial properties and their applicability in inhibiting various foodborne microorganisms and suggests further future investigations regarding their use as possible natural food preservatives that could offer safer, more sustainable methods for extending shelf life. Future research should delve deeper into the use of natural antimicrobials, such as spices, to not only replace synthetic preservatives but also optimize their application in food safety and shelf-life extension. Moreover, there is a need for continuous innovation in encapsulation technologies for antimicrobial agents. Developing cost-effective and efficient methods, along with scaling up production processes, will be crucial to competing with traditional antimicrobial options in terms of both efficacy and affordability.

Insight on Incorporation of Essential Oils as Antimicrobial Substances in Biopolymer-Based Active Packaging.

The increasing interest in microbiological food safety requires the development of sensitive and reliable analyses and technologies for preserving food products' freshness and quality. Different types of packaging systems are one of the solutions for controlling microbiological activity in foods. During the last decades, the development of biopolymer-based active packaging with essential oil incorporation systems has resulted in technologies with exceptional application potential, primarily in the food industry. There is no doubt that this principle can facilitate food status monitoring, reduce food waste, extend the shelf life, improve the overall quality of food, or indicate a larger problem during the storage, production, and distribution of foodstuffs. On the other hand, most antimicrobial packaging systems are in the development phase, while the sensitivity, selectivity, complexity, and, above all, safety of these materials are just some of the essential questions that need to be answered before they can be widely used. The incorporation of essential oils as antimicrobial substances in biopolymer-based active packaging holds significant promise for enhancing food safety, extending shelf life, and offering more sustainable packaging solutions. While challenges exist, ongoing research and innovation in this field are likely to lead to the development of effective and environmentally friendly packaging systems with enhanced antimicrobial properties.

A novel active packaging film containing citronella oil: Preparation, characterization, antimicrobial activity and application in grape preservation

In this study, citronella oil (CO) was incorporated into soy protein isolate (SPI) to prepare antimicrobial protein films capable of inhibiting the growth of spoilage microorganisms on food packaging and providing effective preservation of food products. The morphological, physical and functional properties of the antimicrobial protein films were characterized and investigated by different characterization tools. The SEM results showed that the surface of the films was relatively smooth and flat. FTIR and XRD analyses indicated that CO was uniformly distributed in SPI with good compatibility. Under UV light, the films containing CO showed moderate light transmission and better UV-blocking properties than the SPI-based films. The addition of CO improves the hydrophobic property of the films, and the water content of the film containing 3 % CO is reduced to 20 % compared with that of the SPI film with 27.5 %. The DMA showed that the prepared films had good tensile, swelling and relaxation properties. Antimicrobial protein films containing 3 % CO showed high antimicrobial activity against S. aureus and E. coli. It was also found that the film with 3 % CO had a survival rate of more than 70 % and an apoptosis rate of 5.3 % for BEAS-2B cells after 48 h, indicating that such films are safe. Moreover, the film with 3 % CO showed good freshness and storage capacity with a weight loss of 44.16 % after the grapes had been packed for 7 days. These good physical properties and functional properties suggest that films containing CO have great potential for food packaging applications.

Natural preservatives for hams: Essential oil mixtures and major compounds' efficacy against Clostridium sporogenes

AbstractThe demand for healthier products by consumers has prompted research into the use of essential oils (EOs), which are known for their antimicrobial properties. This study investigated the antimicrobial properties of EOs as a potential alternative to synthetic preservatives, specifically, cinnamon, clove, and oregano EOs, as well as their majority compounds (cinnamaldehyde, eugenol, and carvacrol) on Clostridium sporogenes inoculated in hams. The findings of the study revealed that cinnamon EO and its major compound cinnamaldehyde were the most efficient in inhibiting the growth of C. sporogenes, demonstrating the lowest minimum bactericidal concentration MBC (0.1%). The combination of oregano EO, cinnamon EO, cinnamaldehyde, and carvacrol led to a significant decrease in bacterial growth (approximately 3 log) after 28 days in hams. Furthermore, the presence of spores was not observed for 14 days (at 14°C) and 21 days (at 7°C) of storage, indicating a delay in sporulation. The treatments using the combination of EOs, and their major compounds had minimal impact on the color of the hams while maintaining the physicochemical characteristics of the product. This study demonstrates that EOs and their major compounds can be applied as natural preservatives in ham, offering a potential alternative for reducing the use of nitrites in various food types. The research emphasizes the antibacterial efficacy of cinnamon, clove, and oregano EOs, along with their major compounds, in inhibiting C. sporogenes in ham. The findings indicate that these natural alternatives could be valuable in preserving food products and reducing the reliance on synthetic preservatives.

The Occurrence and Characteristics of Methicillin-Resistant Staphylococcal Isolates from Foods and Containers.

Antimicrobial resistance (AMR) has emerged as an urgent global public health issue that requires immediate attention. Methicillin-resistant staphylococci (MRS) is a major problem, as it may cause serious human and animal infections, eventually resulting in death. This study determined the proportional distribution, genetic characteristics, and antimicrobial susceptibility of mecA- or mecC-carrying staphylococci isolated from food chain products. A total of 230 samples were taken from meat, food, fermented food, and food containers. Overall, 13.9% (32/230) of the samples were identified to have Staphylococcus aureus isolates; of those, 3.9% (9/230) were MRS, with eight mecA-positive and one mecC-positive samples, and 1.3% (3/230) methicillin-resistant Staphylococcus aureus (MRSA). MRSA strains belonging to three sequence types (ST9, ST22, and a newly identified ST), three different spa types (T005, t526, and a newly identified type), and three different SCCmec types (IV, V, and an unidentified SCCmec) were detected. Additionally, eight mecA-positive staphylococcal isolates were identified as S. haemolyticus, S. sciuri, S. simulans, and S. warneri, while the mecC-harboring isolate was S. xylosus. The enterotoxin gene, SEm, was detected at 1.56% in S. aureus, whereas SEq was detected at 0.31%, and SEi was also found in MRSA. Our study emphasizes the importance of enhanced hygiene standards in reducing the risk of occupational and foodborne MRSA infections associated with the handling or consumption of meat, food, and preserved food products.

Edukasi Penggunaan dan Identifikasi Bahan Pengawet pada Produk Pangan di Manunggal Jaya Kecamatan Tenggarong Seberang

Food preservatives have been used in general by the public. The addition of food preservatives intends and intends to prevent the growth of spoilage bacteria in materials and products. The use of preservatives must be in small quantities or within the acceptable threshold. If the amount of preservatives used exceeds the threshold limit, it can trigger health problems. However, many producers abuse the addition of toxic preservatives, which are still found in food, so it is necessary to provide education and practice on detecting preservatives in food in the community, especially in Manunggal Jaya Village, Tenggarong opposite District. This activity begins with outreach and counseling about the types of preservatives and continues with analyzing the detection of dangerous preservatives in food products. The activity results show that the importance of education on preservatives in food products can increase the knowledge and understanding of the community in Manunggal Jaya Village, Tenggarong opposite District, and be able to carry out analyses using a simple method of detecting dangerous preservatives such as formaldehyde and borax.

Emerging Trends and Advancements in the Biopreservation of Fruits

Abstract Fruits are natural, healthy, economically feasible, ready to eat, and provide essential nutrients such as vitamins and minerals, making them a fascinating food. Deterioration of fruits during transportation can cause food security concerns and financial losses. Globally, about 45% of horticultural crops are spoiled and wasted for numerous reasons, such as environmental contamination during growth, harvesting under unsuitable conditions, and improper storage, handling, and display. There are three groups of factors affecting the spoilage of food: physical, chemical, and microbial, which damage the size, color, taste, and texture of fruits. Conventional methods of preserving food products comprise chemical preservation, freezing, drying, and pasteurization, which can result in the loss of nutrients and the addition of unwanted chemicals produced during processing. Therefore, “green” technology is required to preserve fresh produce, which protects and enhances nutritional value in equal measure. This review will present emerging trends and advancements in the biopreservation of fruits, such as lactic acid bacteria, essential oils, herbal extracts, nanoparticles, microcapsules, edible films and coatings, bacteriocins, and bacteriophages. These biopreservative techniques should be easy, inexpensive, eco-friendly, and generally recognized as safe (GRAS) by the World Health Organization (WHO).

Application of Inorganic Anion Sensitive Indicator Electrodes for the Analysis of Food Additives and Preservatives in Food Products

Food chemicals and stabilisers are extensively employed in the food business to improve the nutritional value, safety, and shelf life. However, unnecessary intake can be hazardous to human health. As a result, developing precise and effective techniques for detecting these chemicals in food items is critical. Because of their great choice, sensitivity, and ease of use, artificial anion sensitive indicator electrodes have attracted interest in recent years as a potential technique for the study of food additives and stabilisers. The concepts and uses of inorganic anion sensitive indicator electrodes in the study of preservatives and additives to food in diverse food items are discussed in this research. We also compared the findings acquired with these kinds of electrodes to those generated with other approaches, emphasising their benefits and limits. Finally, we discuss possible uses of this approach in other domains.

Exploring the Power of Thermosonication: A Comprehensive Review of Its Applications and Impact in the Food Industry

Thermosonication (TS) has been identified as a smart remedy for the shortcomings of heat treatment, which typically requires prolonged exposure to high temperatures. This technique combines moderate heat treatment with acoustic energy to eliminate harmful microorganisms and enzymes in food products. Unlike conventional heat treatment, thermosonication utilizes short holding times, allowing for the preservation of food products' phytochemical compounds and sensory characteristics. The benefits and challenges of this emerging technology, such as equipment cost, limited availability of data, inconsistent results, high energy consumption, and scale-up challenges, have been assessed, and the design process for using ultrasound in combination with mild thermal treatment has been discussed. TS has proven to be a promising technique for eliminating microorganisms and enzymes without compromising the nutritional or sensory quality of food products. Utilizing natural antimicrobial agents such as ascorbic acid, Nisin, and ε-polylysine (ε-PL) in combination with thermosonication is a promising approach to enhancing the safety and shelf life of food products. Further research is required to enhance the utilization of natural antimicrobial agents and to acquire a more comprehensive comprehension of their impact on the safety and quality of food products.

The Hydrophobic Extract of Sorghum bicolor (L. Moench) Enriched in Apigenin-Protected Rats against Aflatoxin B1-Associated Hepatorenal Derangement.

Aflatoxin B1 (AFB1) is a recalcitrant metabolite produced by fungi species, and due to its intoxications in animals and humans, it has been classified as a Group 1 carcinogen in humans. Preserving food products with Sorghum bicolor sheath can minimise the contamination of agricultural products and avert ill health occasioned by exposure to AFB1. The current study investigated the ameliorating effect of Sorghum bicolor sheath hydrophobic extract (SBE-HP) enriched in Apigenin (API) on the hepatorenal tissues of rats exposed to AFB1. The SBE-HP was characterised using TLC and LC-MS and was found to be enriched in Apigenin and its methylated analogues. The study used adult male rats divided into four experimental cohorts co-treated with AFB1 (50 µg/kg) and SBE-HP (5 and 10 mg/kg) for 28 days. Biochemical, enzyme-linked immunosorbent assays (ELISA) and histological staining were used to examine biomarkers of hepatorenal function, oxidative status, inflammation and apoptosis, and hepatorenal tissue histo-architectural alterations. Data were analysed using GraphPad Prism 8.3.0, an independent t-test, and a one-way analysis of variance. Co-treatment with SBE-HP ameliorated an upsurge in the biomarkers of hepatorenal functionality in the sera of rats, reduced the alterations in redox balance, resolved inflammation, inhibited apoptosis, and preserved the histological features of the liver and kidney of rats exposed to AFB1. SBE-HP-containing API is an excellent antioxidant regiment. It can amply prevent the induction of oxidative stress, inflammation, and apoptosis in the hepatorenal system of rats. Therefore, supplementing animal feeds and human foods with SBE-HP enriched in Apigenin may reduce the burden of AFB1 intoxication in developing countries with a shortage of effective antifungal agents.

Mānuka Oil vs. Rosemary Oil: Antimicrobial Efficacies in Wagyu and Commercial Beef against Selected Pathogenic Microbes

Essential oils possessing antimicrobial characteristics have acquired considerable interest as an alternative to chemical preservatives in food products. This research hypothesizes that mānuka (MO) and kānuka (KO) oils may possess antimicrobial characteristics and have the potential to be used as natural preservatives for food applications. Initial experimentation was conducted to characterize MOs (with 5, 25, and 40% triketone contents), rosemary oil (RO) along with kanuka oil (KO) for their antibacterial efficacy against selected Gram-negative (Salmonella spp. and Escherichia coli), and Gram-positive (Listeria monocytogenes and Staphylococcus aureus) bacteria through disc diffusion and broth dilution assays. All MOs showed a higher antimicrobial effect against L. monocytogenes and S. aureus with a minimum inhibitory concentration below 0.04%, compared with KO (0.63%) and RO (2.5%). In chemical composition, α-pinene in KO, 1, 8 cineole in RO, calamenene, and leptospermone in MO were the major compounds, confirmed through Gas-chromatography-mass spectrometry analysis. Further, the antimicrobial effect of MO and RO in vacuum-packed beef pastes prepared from New Zealand commercial breed (3% fat) and wagyu (12% fat) beef tenderloins during 16 days of refrigerated storage was compared with sodium nitrate (SN) and control (without added oil). In both meat types, compared with the SN-treated and control samples, lower growth of L. monocytogenes and S. aureus in MO- and RO- treated samples was observed. However, for Salmonella and E. coli, RO treatment inhibited microbial growth most effectively. The results suggest the potential use of MO as a partial replacement for synthetic preservatives like sodium nitrate in meats, especially against L. monocytogenes and S. aureus.

Properties of cheese and ground beef in the presence of staghorn sumac

AbstractCost‐effective and under‐exploited plant species as functional food ingredients represent alternatives to chemicals with safety concerns and potent toxicity. Staghorn sumac (Rhus typhina) is considered a good source of natural polyphenols, which can be introduced into food products to carry out some specific technological functions (e.g., antioxidation and preservation). However, sumac fruit as a spice is under‐explored for its diversified uses in various food products as a novel functional ingredient. This study explored the technological feasibility and sensory acceptability of two representative sumac‐fortified food systems, namely Cheddar cheese and ground beef. Extracts of sumac (0.5%−5%) dose‐dependently increased the total phenolic and anthocyanin contents, while reducing thiobarbituric acid reactive substance (up to 46%), total plate counts of bacteria (up to 4.5 log colony‐forming units [CFU]/g) and pH of the cheese. In fresh ground beef, sumac extract at concentrations of 1%−5% was effective in reducing the total aerobic counts of Escherichia coli inoculated fresh ground beef by 0.16−1.14 log CFU/g, while improving meat color and oxidative stability during a storage period of 7 days at 4°C. Phytochemical profiling by high‐performance liquid chromatography showed the presence of gallic acid, caffeic acid, ellagic acid, quercetin and anthocyanins in the sumac extracts. These compounds were possibly responsible for the antimicrobial and antioxidant activities in the evaluated food systems. Sensory acceptability of Cheddar cheese or cooked beef meatballs with sumac extract addition (0.5%−3%) were similar to their respective controls. This study provides a feasible approach to satisfy health‐conscious consumers who favor a natural multifunctional ingredient to preserve food products.

Fabrication, characterisation and antimicrobial activity of electrospun Plantago psyllium L. seed gum/gelatine nanofibres incorporated with Cuminum cyminum essential oil nanoemulsion

SummaryPlantago psyllium L. seed gum (PPSG)/gelatine nanocomposites containing Cuminum cyminum essential oil (CCEO) were prepared via electrospinning, and the antibacterial properties of the electrospun nanofibres were assessed against Staphylococcus aureus. The nanoemulsion was prepared by adding CCEO to the PPSG/gelatine mixture and sonicated. Uniform nanofibres resulted from the nanoemulsion containing 1.5% PPSG, 8% gelatine and 3% CCEO, and no chemical reactions between the components of the electrospun nanofibres were detected. Growth inhibition zone diameter indicated that the electrospun nanofibres containing at least 3% CCEO had the most significant inhibitory effect on the growth of S. aureus. The electrospun PPSG/gelatine‐CCEO nanocomposites are capable of being used as a biodegradable material in food packaging as well as in edible coatings for the preservation of food products.

A comprehensive review on the application of ultrasound for the preservation of fruits and vegetables

AbstractUltrasound technology is a green and environment‐friendly technology that rarely causes thermal damage to food products. In this review, we present an overview of the application of ultrasound technology for the dehydration of food products, including pre‐treatments such as ultrasound, osmotic dehydration, and ultrasound‐assisted osmotic dehydration. Dehydration promotes the preservation of food products and increases the shelf life of the food products by reducing water activity. In addition, the ultrasound‐assisted drying of food products is highlighted. The influence of ultrasound and ultrasound‐assisted osmotic dehydration parameters on drying kinetics and food quality is also discussed. The application of pre‐treatments enhances the mass transfer rate, increases the drying rate and drying efficiency, reduces drying time, and helps to retain food properties such as texture and nutrition value. Generally, ultrasound and osmotic dehydration application decreases water activity, increases rehydration ability, improves product color, and reduces nutrient losses. To conclude, we have discussed the use of ultrasound applications for improving the quality and safety of produce before and after the drying process.

A Concise Profile of Gallic Acid-From Its Natural Sources through Biological Properties and Chemical Methods of Determination.

Nature is a valuable source of anti-oxidants that have a health-promoting effect by inhibiting various undesirable changes leading to cell degradation and, consequently, potential disease ailments. One of them is gallic acid which has been used as a healing agent since ancient times. Currently, due to various beneficial properties, this compound is considered to be one of the main phenolic acids of great importance in numerous industries. It is commonly used as a substance protecting against the harmful effects of UV radiation, an astringent in cosmetic preparations, and a preservative in food products. Therefore, gallic acid is now deemed essential for both human health and industry. Increasingly better methods of its isolation and analysis are being developed, and new solutions are being sought to increase its production. This review, presenting a concise characterization of gallic acid, updates the knowledge about its various biological activities and methods used for its isolation and determination, including chromatographic and non-chromatographic methods.