Preservation Period Research Articles

Appeals to God in the documents of the law enforcement system of Russia (from ancient times to 1917)

Documents of the domestic law enforcement system from the time of Kievan Rus to 1917 are considered for turning to God – the source of immutable moral values from the standpoint of the Orthodox religious tradition. The study of this topic is of particular importance for understanding Russian history, taking into account the factor that Orthodoxy occupied in the life of the state and the individual. It is noted that the origins of the Russian law enforcement sphere coincide with the emergence of Christianity and statehood in Russia. On the basis of the anthology, published on the occasion of the 300th anniversary of the Russian police, and through the study of documents from the 10th to the 19th centuries, the nature of such appeals is investigated, and their comparative analysis is carried out. Three periods are distinguished: the first – from the first treaty between Russia and the Greeks until the introduction of the post of St. Petersburg General Police Chief; the second – from the introduction of the post of St. Petersburg General Police Chief to the establishment of the Ministry of Internal Affairs; the third is from the establishment of the Ministry of Internal Affairs to the disbandment of the Gendarme Corps in 1917. The documents of each of the periods analyze the forms and context of appeals to God, which made it possible to identify the trend of changes taking place in society in relation to the highest value for the Orthodox faith – the Triune God. The conclusion is made about the period of preservation of the traditional for the Orthodox faith concepts of God in the documents of the law enforcement system of Russia, as well as the time of the beginning of the loss of such concepts. A hypothesis has been put forward about the possibility of reviving the highest traditional religious values in Russia at the present stage.

Development of an estimation-experimental method for estimating the preservation indicators of single-use articles

To manage the operation of modern single-use products, it is necessary to evaluate their preservation indicators as uncontrolled, non-repairable, and maintenance-free objects. Data for assessing its parameters are considered as one-time censored samples with continuous monitoring, which does not correspond to the mode of storage of products during operation. Under the conditions of limited volumes of censored samples, it is problematic to identify the parametric model of persistence. To solve this problem, a non-parametric estimation-experimental method has been devised, which is a set of models for data generation, estimation of the function of the distribution of the preservation period and preservation indicators. The data generation model is represented by a scheme of operational tests and analytical relationships between the quantities of tested and failed articles. The model of estimating the distribution function describes the process of its construction on the generated data. Models for estimating preservation indicators are represented by ratios for their point and interval estimates, as functionals from the restored distribution function. Unlike the well-known ones, the developed method implements the assessment of indicators under the conditions of combined censorship. The method can be used to assess the preservation indicators of single-use articles with an error of at least 7 %. At the same time, their lower confidence limits are estimated at 0.9 with an error not worse than 14 % with a censorship degree of not more than 0.23. The restored distribution function agrees well (reliability 0.9, error 0.1) with the actual persistence of articles with censorship degrees of not more than 0.73, which is acceptable for solving the problems of managing their operation.

Ball milling and hot-melt extrusion of indomethacin–l-arginine–vinylpyrrolidone-vinyl acetate copolymer: Solid-state properties and dissolution performance

Commonly applied approaches to enhance the dissolution properties of low water-soluble crystalline active pharmaceutical ingredients (APIs) include their amorphization by incorporation into a polymeric matrix and the formation of amorphous solid dispersions, or blending APIs with low-molecular-weight excipients and the formation of a co-amorphous system. This study focused on the preparation and characterization of binary (consisting of indomethacin (IND) and polymer – copovidone (PVP VA 64), as a carrier, or amino acid – L-arginine (ARG), as a co-former) and ternary (comprising the same API, polymer, and amino acid) formulations. Formulations were produced by ball milling (BM) and/or hot-melt extrusion (HME), and extensive physicochemical characterization was performed. Specifically, the physicochemical and solid-state properties of a model IND–ARG system incorporated into a polymeric matrix of PVP VA 64 by HME and BM as well as by combined BM/HME method together with the impact of the preparation strategy on the dissolution profiles and long-term physical stability were investigated. Ball-milled binary and ternary formulations were found to be amorphous. The residual crystals corresponding to IND–ARG salt were identified in the ternary formulations produced via HME. Despite the presence of a crystalline phase, dissolution tests showed that ternary systems prepared by HME exhibited improved IND solubility when compared to pure crystalline IND and their corresponding physical mixture. None of the binary and ternary formulations that were initially fully amorphous did undergo recrystallization during the entire period of preservation (minimum of 12 months) in dry conditions at 25 °C.

Effect of different plants’ aromatic essential oils on frozen Awassi lamb meat’s chemical and physical characteristics

The effect of drenching Awassi lambs with three aromatic essential oils from sage (Salvia officinalis L.), clove (Syzygium aromaticum L.), and laurel (Laurus nobilis L.) was investigated on meat chemical and physical characteristics, and oxidative and deterioration measurements. Twenty-four Awassi lambs, five to six months old, were divided into four groups. A concentrated diet was provided to the lambs at a rate of 3% of the body weight. The treatments were as follows: T1 was served as the untreated control, while T2, T3, and T4 were drenched with oils of sage, clove, and laurel, respectively. Drenching was carried out using water-soluble capsules containing 500 mg oil/capsule/day. Treatments lasted 90 days. At the end of the treatment period, the animals were fasted overnight and slaughtered. The carcasses were cleaned and kept at 4°C for 24 h. The longissimus dorsi (LD) muscle was then separated and preserved in a plastic bag for three preservation periods: no freezing and 30 days and 60 days freezing at −18°C. Several physical, fat, and protein stability analyses of meat were done after the preservation periods. The results indicated no significant effect of drenching Awassi lambs with different aromatic essential oils on the meat’s physical and chemical characteristics. However, these oils, especially clove oil, affected fat and protein stability with increasing preservation period by freezing.

Study of The Inhibitory Activity of The Alcoholic Extract of Licorice Plant Glycyrrhiza Glabral and Its Effect on Prolonging The Storage Period of Manufactured Biscuit

The study was conducted to obtain the alcoholic extract of the licorice roots plant and to test its effectiveness against diagnosed bacterial isolates. This research was conducted at the Food Contamination Research Center/Department of Environment and Water/Ministry of Science and Technology. The results showed the following: 1-80% ethyl alcohol was used to obtain the alcoholic extract using a saxolite device, and the activity of the extract was tested against four bacterial isolates diagnosed in the Food Contamination Research Center, which included two gram-negative isolates of Escherichia Coli and Pseudomonas aeruginosa, and two gram-positive isolates of Bacillus Cereus and Staphylococcus aeastureus by a method, in addition to yeast by Diffusion method. The study included two treatments of biscuits with alcoholic extract (A) at a concentration of 10% and compared to the control treatment (B) without any adding and for preservation periods (1, 4, 8, 15, 22) days, and microbial tests were conducted for the biscuits. The alcoholic extract showed a lethal activity to microorganisms and no bacterial cell appeared in the biscuit samples except for the incubation period (22) days for storing biscuits, which recorded the presence of fungi (2) cells/ml. Conducting sensory evaluation of the treatments (A, B) for biscuits where there were no significant differences (P<0.05) for sensory attributes except for flavor and flakes of biscuits.

Ex vivo lung perfusion.

Lung transplantation is a life-saving treatment for patients with end stage lung disease. The imbalance between lung graft supply and recipients has been a serious issue and barrier to successful lung transplantation. Ex vivo lung perfusion is a strategy wherein lungs are perfused and ventilated outside of the body. This technology has emerged as a safe preservation method that also enables the reassessment and reconditioning of marginal lung grafts. Ex vivo lung perfusion has successfully expanded the donor pool and led to greater lung transplant activity worldwide. Furthermore, ex vivo lung perfusion can be used as a platform for advanced diagnostics that enable specific targeted or personalized treatments that can be developed along a bench to bedside pathway leading to safe ex vivo intervention. Recent findings have shown that ex vivo lung perfusion could significantly and safely extend the preservation period, which enables transplant programs further optimization of the logistics around transplantation surgeries, and create a new paradigm whereby donor lungs are assessed at a centralized ex vivo lung perfusion center prior to delivery to a transplant clinic in need. The introduction of ex vivo lung perfusion to clinical lung transplantation has been a major step in the evolution and practice of lung transplantation.

Mitochondrial respiratory chain and Krebs cycle enzyme function in human donor livers subjected to end-ischaemic hypothermic machine perfusion.

IntroductionMarginal human donor livers are highly susceptible to ischaemia reperfusion injury and mitochondrial dysfunction. Oxygenation during hypothermic machine perfusion (HMP) was proposed to protect the mitochondria but the mechanism is unclear. Additionally, the distribution and uptake of perfusate oxygen during HMP are unknown. This study aimed to examine the feasibility of mitochondrial function analysis during end-ischaemic HMP, assess potential mitochondrial viability biomarkers, and record oxygenation kinetics.MethodsThis was a randomised pilot study using human livers retrieved for transplant but not utilised. Livers (n = 38) were randomised at stage 1 into static cold storage (n = 6), hepatic artery HMP (n = 7), and non-oxygen supplemented portal vein HMP (n = 7) and at stage 2 into oxygen supplemented and non-oxygen supplemented portal vein HMP (n = 11 and 7, respectively). Mitochondrial parameters were compared between the groups and between low- and high-risk marginal livers based on donor history, organ steatosis and preservation period. The oxygen delivery efficiency was assessed in additional 6 livers using real-time measurements of perfusate and parenchymal oxygen.ResultsThe change in mitochondrial respiratory chain (complex I, II, III, IV) and Krebs cycle enzyme activity (aconitase, citrate synthase) before and after 4-hour preservation was not different between groups in both study stages (p > 0.05). Low-risk livers that could have been used clinically (n = 8) had lower complex II-III activities after 4-hour perfusion, compared with high-risk livers (73 nmol/mg/min vs. 113 nmol/mg/min, p = 0.01). Parenchymal pO2 was consistently lower than perfusate pO2 (p ≤ 0.001), stabilised in 28 minutes compared to 3 minutes in perfusate (p = 0.003), and decreased faster upon oxygen cessation (75 vs. 36 minutes, p = 0.003).ConclusionsActively oxygenated and air-equilibrated end-ischaemic HMP did not induce oxidative damage of aconitase, and respiratory chain complexes remained intact. Mitochondria likely respond to variable perfusate oxygen levels by adapting their respiratory function during end-ischaemic HMP. Complex II-III activities should be further investigated as viability biomarkers.

One-Pot Synthesis of Bright Blue Luminescent N-Doped GQDs: Optical Properties and Cell Imaging.

High fluorescent graphene quantum dots (GQDs) are promising in bioimaging and optoelectronics. In this paper, bright blue fluorescent N-doped GQDs were synthesized using a ultrasonic-assisted hydrothermal method. The morphology, structure, surface chemistry, optical properties, and stability subject to photo-bleaching, temperature, pH and preservation period for the N-GQDs were investigated in detail using various microscopy and spectroscopy techniques. The results showed that the N-GQDs possessed an average size of 2.65 nm, 3.57% N doping, and up to 54% quantum yield (QY). The photoluminescence (PL) spectra of the N-GQDs are excitation dependent when excited in the range of 300–370 nm and excitation independent in the range of 380–500 nm for the core and surface states emission. The N-GQDs showed excellent photo-bleaching resistance and superior photo-stability. At room temperature and in the pH range of 3–8, the fluorescence of the N-GQDs was almost invariable. The N-GQDs can be stably preserved for at least 40 days. The average decay lifetime of the N-GQDs was 2.653 ns, and the radiative and nonradiative decay rate constants were calculated to be 2.04 × 108 s−1 and 1.73 × 108 s−1, respectively. The PL mechanism was qualitatively explained. The N-GQDs was used for cell imaging, and it showed good results, implying great potential applications for bioimaging or biomarking.

Holocene coastal evolution, past tsunamis, and extreme wave event reconstructions using sediment cores obtained from the central coast of Hidaka, Hokkaido, Japan

Investigating coastal areas far from tsunami sources can improve the understanding of the magnitude and origin of giant tsunamis. However, distinguishing tsunami deposits from extreme storm deposits is more challenging since the layers are less prominent compared to the those in the locations near a tsunami source. This study reconstructed the Holocene coastal environments on the central coast of Hidaka, Hokkaido, ~250 km from the Kuril Trench. Sand layers induced by extreme waves were identified by examining the sea-level index points in the late to mid-Holocene by diatom-assemblage analysis through the combination of chemical analyses with the investigation of sediment cores. Detailed facies analyses on sediment cores obtained from several transects showed that sediment preservation differs according to the location of the cores relative to the ambient coastline. In the zone behind the beach ridge, the paleoenvironments changed from a saltmarsh formed by transgression to a swamp closed by a beach ridge formed by sea regression in the mid-Holocene. Conversely, the seaward zone indicated various environmental changes due to meandering rivers and the development of the beach ridge system due to sea regression. We found four volcanic ash layers and seven sand layers with clear basal contact with peat or organic mud. According to their sedimentological features, these sand layers were formed by tsunamis or storm surges; at least four of the sand layers are likely to be tsunami deposits, considering the ages of tsunami deposits in the surrounding region. Dramatic changes in the depositional environment associated with relative sea-level changes resulted in a short period of preservation of the sand layers. In addition, their distributions were limited, ranging from a few tens of meters to hundreds of meters from the beach ridge. Thus, in an area far from tsunami sources, the traces of extreme waves may be overlooked if the coastal evolutional process is not considered.

Comparison of the Effects of Temperature and Dehydration Mode on Glycerin-Based Approaches to SMILE-Derived Lenticule Preservation

The aim of this study was to explore the optimal method of small-incision lenticule extraction (SMILE)-derived lenticules, subjected to long-term preservation using glycerol, under a range of temperatures, and using an array of dehydration agents. In total, 108 myopic lenticules were collected from patients undergoing the SMILE procedure. Fresh lenticules served as a control group for this study, whereas all other lenticules were separated into 8 groups, which were preserved at 4 different temperatures (room temperature [RT], 4, -20, and -80°C) with or without silica gel in anhydrous glycerol. Evaluated parameters included thickness, transmittance, hematoxylin and eosin staining, transmission electron microscopy, and immunohistochemistry analyses. After a 3-month preservation period, lenticular thickness in these different groups was significantly increased, particularly for samples stored at RT. The mean percentage transmittance of lenticules stored at -80°C with or without silica gel was closest to that of fresh lenticules. Hematoxylin and eosin staining revealed sparsely arranged collagen fibers that were more scattered in preserved lenticules relative to fresh lenticules, particularly in RT samples. Transmission electron microscopy revealed that the fibril bundles densities in lenticules stored at RT were significantly less than those stored at other temperatures. Immunohistochemistry analyses revealed reductions in or loss of CD45 and human leukocyte antigens in all preserved lenticules relative to control samples. Of the tested approaches, the preservation of SMILE-derived lenticules over a 3-month period was optimal at -80°C with or without silica gel in anhydrous glycerol.

Persufflation—Current State of Play

With the ever-increasing disparity between the number of patients waiting for organ transplants and the number organs available, some patients are unable to receive life-saving transplantation in time. The present, widely-used form of preservation is proving to be incapable of maintaining organ quality during long periods of preservation and meeting the needs of an ever-changing legislative and transplantation landscape. This has led to the need for improved preservation techniques. One such technique that has been extensively researched is gaseous oxygen perfusion or Persufflation (PSF). This method discovered in the early 20th century has shown promise in providing both longer term preservation and organ reconditioning capabilities for multiple organs including the liver, kidneys, and pancreas. PSF utilises the organs own vascular network to provide oxygen to the organ tissue and maintain metabolism during preservation to avoid hypoxic damage. This review delves into the history of this technique, its multiple different approaches and uses, as well as in-depth discussion of work published in the past 15 years. Finally, we discuss exciting commercial developments which may help unlock the potential for this technique to be applied at scale.

A comparative study between different preservation methods on the viability of some yeast cultures

The influence of chilling, freezing, and dry-ing preservation methods on morphological properties and viability of four yeast cultures, isolated Saccharomyces boulardii (SB1 and SB2), Saccharomyces cerevisiae (SC), and Kluyveromyces lactis (KL), was studied. chilling preservation had no effect on yeast colony morphology parameters till the end of the preservation period in contrast with freez-ing and drying preservation methods. Drying preservation resulted in the highest viability decrement % of yeast cultures ranged between 59.38 to 40.21% as compared with other preservation methods. There is a noticeable re-duction in D values of preserved yeast cultures by drying, which varied from (33 to 26 day) in comparison to chilling and freezing preserva-tion that recorded (119 to 83 and 180 to 57 day), respectively. The obtained results re-vealed that chilling preservation under proper conditions, as well as freezing, is more prefer-able for the preservation of studied yeast cul-tures than that preserved yeast by the drying method.

Влияние замораживания семян кенафа (Hibiscus cannabinus L.) на всхожесть, рост и развитие растений

Abstract A comparative study of the effect of different storage regimes of seeds (at + 5 ° С, -20 ° С and under deep freezing conditions at -196 ° С) of a valuable bast fiber culture - kenaf - Hibiscus cannabinus L. on their viability and the development of plants from them in field conditions. Freezing increases the laboratory and field germination of kenaf seeds, and also increases the energy of their germination. It did not cause the appearance of non-viable, ugly seedlings and plants, as well as their death. Plants went through a full development cycle and set full-fledged seeds. Biometric parameters of plants obtained from thawed seeds and control ones are very close. Cryopreservation is a promising way of storing seeds of the studied species, since it ensures longer periods of preservation of their viability. Keywords: KENAF, SEED BANKS, LONG-TERM SEED STORAGE REGIMES, CRYOCONSERVATION

Characterization and application of dried neem leaf powder as a bio-additive for salt less animal skin preservation for tanneries.

Sodium chloride (NaCl) is commonly used as a curing/preservative agent for raw hides and skins in tanneries and is removed through a soaking process with total dissolved solids (TDS) and other organic pollutants in effluent, causing significant pollution load to the environment. Hence, the present study evaluated to apply dried neem leaf powder (DNL) as an additive to reduce the usage of salt in skin processing and preservation. To make certain of DNL antimicrobial properties, solvent extracts were performed against proteolytic bacteria isolated from raw skins. Initial characterization of DNL revealed the presence of bioactive compounds nimbolide and dehydro salannol and acetone extract with 16.9-mm, 10-mm and 8-mm zone of inhibition against Salmonella sp., E. coli sp. and Bacillus sp. identified using phenotypic conventional biochemical screening method. Further, skin curing experiments were carried out using four different treatments of DNL (10% 15%, 20% and 25% w/w) along with 15% w/w of conventional salt to obtain an optimum concentration for pilot-scale studies. Thus, the application of optimal DNL (15%) and salt (15%) resulted in no physical changes such as smell and hair slip and was taken for further studies for hydroxyproline activity, pollution load and organoleptic properties along compared with control 40% salt. DNL-aided salt less preservation of freshly flayed goat skins at ambient condition showed no hair slip or putrefaction during the preservation period with significant reduction of TDS (86%) and chloride (71%) in soak liquors compared to conventional salt preservation and enhanced organic load requiring additional treatment. However, the application of the organoleptic, physical and hydrothermal properties of resulting leathers produced from the DNL applied skins was on par with results of leather obtained from conventional salt. Thus, our results demonstrate DNL-aided salt less preservation method is able to reduce the amount of salt for preservation of goat skins significantly, leading to reduced salinity issues during leather processing.

Effect of devitrification on the survival and resistance of dried Saccharomyces cerevisiae yeast.

Yeasts are anhydrobiotes that accumulate large amounts of trehalose, which is involved in the vitrification of the cytoplasm during drastic desiccation. The effect of devitrification, which can be induced by the transient exposure of desiccated yeasts to increased humidity or elevated temperature, on the survival of yeast has been studied. A glass transition temperature (Tg)/water activity (aw) diagram of yeast was constructed based on differential scanning calorimetry analysis. The survival rate of yeasts that were equilibrated at different relative humidities (RHs) and temperature values over their Tg range was measured. The results revealed a long period of cell preservation at an intermediate RH (55%), with 100% survival observed after 3 months, a loss of 1.24 log colony-forming units/g recorded after 1 year at 25 °C and full preservation of viability at 75 °C for 60 min and at 100 °C and 12% RH for up to 10 min. These findings led us to conclude that dried yeast can resist low or intermediate RH values and elevated temperatures in the devitrified state. Considering the thermal and humidity fluctuations occurring in the yeast environments, we hypothesized that the supercooled state, which occurs immediately above the Tg after rehydration or heating, is a protective state that is involved in the persistence of yeasts at intermediate humidity levels. KEY POINTS: • Yeast survival for months in a supercooled state is observed at room temperature. • Dried yeasts survive a 10-min exposure to 100 °C in the supercooled state. • The supercooled state is suitable for yeast preservation.

Protective effect of estradiol copreservation against kidney ischemia-reperfusion injury.

Ischemia-reperfusion injury (IRI) is the major cause of delayed graft function (DGF) during the posttransplantation period. Estradiol (E2) prevents IRI-induced kidney dysfunction and tissue injury. However, many side effects limit E2's in vivo application. Recent evidence uncovers E2's expanded use in the field of transplantation. We aimed to study if and how E2 exerts protective activity during the period of kidney organ preservation. The autologous kidney transplant model in rats was first established. Rats were divided into 5 groups: normal group (N), sham group (sham), static cold storage (SCS) 4hours group (control), SCS 4hours+ethanol (1µL/mL) group (solvent), and SCS 4hours+ethanol (1µL/mL)+E2 (1000ng/mL) group (E2). ERα expression under hypothermia was measured by western blotting. Moreover, biochemical analyses of plasma levels of creatinine, BUN, estradiol, and testosterone were examined. Among all groups, kidney tissues were collected and processed for further western blot analysis about ERα, eNOS, Bcl-2, and Bax expression, histological analyses such as H&E staining to evaluate pathological severity. Inaddition, a TUNEL assay is performed to evaluate apoptosis. E2 copreservation upregulated ERα expression under hypothermia. Moreover, E2 copreservation reduced levels of creatinine and BUN in plasma but without affecting estradiol and testosterone. Further, E2 copreservation increased expression of eNOS and antiapoptotic Bcl-2 and decreases expression of proapoptotic Bax. E2 copreservation significantly inhibited IRI-induced apoptosis and evidently improved pathological severity in the kidney of rats. E2 copreservation exerts protective activity against IRI-induced pro-inflammatory and proapoptotic effects in kidneys during organ preservation time and improves transplanted kidney function.

Влияние замораживания семян Abutilon theophrasti Medik. и Eruca vesicaria (L.) Cav. на всхожесть, рост и развитие растений

A comparative study of the effect of different storage regimes of seeds (at + 5 ° С, -20 ° С and under deep freezing conditions at -196 ° С) of two cultivated plants of the Abutilon theophrasti Medik. and Eruca vesicaria (L.) Cav. on their viability and the development of plants from them in the field was carried out. Freezing increases the laboratory and field germination of the seeds of the A. theophrasti and does not significantly affect the germination of the seeds of the A. theophrasti, and also increases the germination energy of the seeds of both plant species. It did not cause the appearance of non-viable, ugly seedlings and plants. Plants went through a full development cycle and set full-value seeds. Biometric parameters of plants obtained from thawed seeds and control ones are very close. Cryopreservation is a promising way of storing seeds of the studied species, since it ensures longer periods of preservation of their viability. Keywords: ABUTILON THEOPHRASTI; ERUCA VESICARIA; SEED BANKS; LONG-TERM SEED STORAGE REGIMES; CRYOPRESERVATION

ANTIBACTERIAL ACTIVITY OF PEPPERMINT, BASIL AND ROSEMARY ESSENTIAL OILS OBTAINED BY STEAM DISTILLATION

The aim of this paper is to present the antibacterial activity of peppermint, basil and rosemary essential oils obtained by steam distillation after the preservation period using various methods. The processing methods used for the plant materials were natural drying, freezing and gamma ray irradiation. The oils were extracted after certain preservation periods from Mentha piperita, Ocimum basilicum and Rosmarinus officinalis aromatic plant materials. The antibacterial activity of the studied essential oils was assessed against 8 bacterial strains (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus viridans, Enterococcus faecalis, Streptococcus pneumoniae, Escherichia coli, Klebsiella pneumoniae and Serratia marcescens). Antibacterial activity of the essential oils was investigated using disc diffusion method. The results showed that the antibacterial activity depends on the type of aromatic plant and the type of used preservation methods. The most active essential oils against the tested bacteria were, in descending order rosemary, peppermint and basil.

Effect of composite coating treatment and low-temperature storage on the quality and antioxidant capacity of Chinese jujube (Zizyphus jujuba cv. Junzao)

The purpose of this study was to explore the effects of coating treatment using a composite of 1-methylcyclopropene (1-MCP), chitosan and natamycin (NATA) on the quality and antioxidant capacity of Chinese jujube (Zizyphus jujuba cv. Junzao) stored at different temperatures (Room temperature and 0 ± 1 °C). Respiration, ethylene release, and decay rates; Malondialdehyde (MDA); total phenol, flavonoid, and Ascorbic Acid (AsA) contents; Peroxidase (POD), Polyphenol Oxidase (PPO), Superoxide Dismutase (SOD), and Ascorbate Peroxidase (APX) activities; and free-radical scavenging ability were considered. The results showed that the low-temperature composite coating treatment of Chinese jujube effectively inhibits its decay and MDA accumulation; delays its respiration and ethylene release peaks; increases the activities of the antioxidant enzymes POD, SOD, and APX; and inhibits its PPO activity. Furthermore, the jujube retains its high concentrations of total phenols, flavonoids, and AsA as well as its free-radical-scavenging rate into the later storage period. Thus, the low-temperature composite coating treatment is an effective method for improving the postharvest quality of Chinese jujube and extending its preservation period.

Perbandingan Teknik Penyimpanan Menggunakan Medium Yang Berbeda Terhadap Viabilitas Kapang Colletotrichum capsici dan Prycularia oryzae

Laboratory of Microbiology which is part of Basic Laboratory System in Faculty of Mathematics and Natural Sciences University of Lambung Mangkurat have commonly used as a place of various activities both science academic practice and research. This laboratory also provides analysis for samples and production. Microbial culture plays important rule in practical science of microorganism. Thus, continuous culture and preservation are highly required. Preservation and re-culture of microbes in the laboratory recently using short-term preservation and periodic re-culture to a new medium, so it is necessary to conduct research on microbial preservation, especially molds with certain preservation techniques using different media. Colletotrichum capsici and Prycularia oryzae isolates were used in this study. The two molds were stored using different mediums: tapioca, 50% PDB + glycerol, and 50% PDB + liquid paraffin with a preservation period up to 1-3 months. The preserved mold was then cultured onto PDA medium to see its viability. The results of preservation of Colletotrichum capsici for 1-3 months using 3 different types of medium showed that 50% of liquid PDB-paraffin medium had the best viability compared to tapioca medium and 50% PDB-glycerol. Prycularia oryzae preserved for 1-3 months using tapioca medium, 50% PDB-glycerol, and 50% liquid PDB-paraffin cannot grow back.