Storage Preservation Research Articles

A method to enhance privacy preservation in cloud storage through a three-layer scheme for computational intelligence in fog computing

Recent advancements in cloud computing have heightened concerns about data control and privacy due to vulnerabilities in traditional encryption methods, which may not withstand internal attacks from cloud servers. To overcome these issues about the data privacy and control of transfer on cloud, a novel three-tier storage model incorporating fog computing method has been proposed. This framework leverages the advantages of cloud storage while enhancing data privacy. The approach uses the Hash-Solomon code algorithm to partition data into distinct segments, distributing a portion of it across local machines and fog servers, in addition to cloud storage. This distribution not only increases data privacy but also optimises storage efficiency. Computational intelligence plays a crucial role by calculating the optimal data distribution across cloud, fog, and local servers, ensuring balanced and secure data storage.•Experimental analysis of this mathematical mode has demonstrated a significant improvement in storage efficiency, with increases ranging from 30 % to 40 % as the volume of data blocks grows.•This innovative framework based on Hash Solomon code method effectively addresses privacy concerns while maintaining the benefits of cloud computing, offering a robust solution for secure and efficient data management.

Antimicrobial Potential of Cedrus deodara Essential Oil to Preservative Effect for the Vegetables and Fruits

Abstract A unique species of pine, Cedrus deodara is known for its wood oil. Its traditional therapeutic use is mainly antibacterial and anti-inflammatory. The aim of this study was to investigate the antibacterial properties of Cedrus deodara essential oil (CDEO) obtained from the crushed wood. The antimicrobial activity of CDEO was evaluated against Gram-negative (G-) bacteria which included Pseudomonas aeruginosa CCM 1595, Salmonella enterica subs. enterica CCM 3807 and Gram-positive (G+) bacteria Yersinia enterocolitica CCM 5671. Listeria monocytogenes CCM 4699, Staphylococcus aureus subs. aureus CCM 2461 and Streptococcus consellatus CCM 4043 in vitro and in situ. The best antimicrobial activity for the disc diffusion method ranged from 4.67 to 9.67 mm and the minimum inhibitory concentration ranged from 1.48 to 5.44 mg.mL-1. The most effective antimicrobial effect was found against S. aureus and L. monocytogenes. The vapour phase used showed the best antimicrobial effect against P. aeruginosa in the kiwifruit model and L. monocytogenes in the banana model at a lower CDEO concentration of 62.5 µg.L-1 and against P. aeruginosa in the potato model and Y. enterocolitica in the cucumber model at a higher CDEO concentration of 500 µg.L-1. CDEO showed good antimicrobial activity against bacteria on vegetable and fruit model and may be a new preservative for storage of vegetables and fruits.

Preservation of coagulation function by normothermic machine perfusion in liver transplant as evidenced by thromboelastography parameters.

The use of normothermic machine perfusion (NMP) over static cold storage in liver transplantation has been shown to reduce posttransplant risks of early allograft dysfunction, primary nonfunction, and ischemic cholangiopathy, and its increasing use has played a role in the expanded utilization of marginal livers. While studies have demonstrated improved clinical outcomes using NMP over static cold storage preservation, real-time intraoperative data reflecting the quality and viability of NMP livers is limited. This retrospective, single-center study compared NMP versus static cold storage livers in first-time recipients of liver transplants through the evaluation of synthetic coagulation function as measured by thromboelastography and conventional coagulation testing. Secondarily, transfusion utilization between the 2 cohorts was reviewed. One hundred eighty-six recipients of liver transplants receiving allografts from donors after circulatory death were included in the study, of which 99 (53%) allografts were preserved in static cold storage, and 87 (47%) allografts were placed on the TransMedics Organ Care System. Study findings showed NMP livers supported with the TransMedics Organ Care System were associated with increased synthetic coagulation function and less excess fibrinolysis in the postreperfusion period compared to static cold storage livers, and that these findings were better reflected in real-time with thromboelastography monitoring versus conventional coagulation testing. Following reperfusion, there was a significant decrease in the transfusion of blood products in the NMP group compared with that in the static cold storage group. Overall, we determined that the use of intraoperative thromboelastography can provide real-time data to assess one aspect of reperfusion liver quality and viability.

Spraying glycine betaine on fresh pistachio fruits (Pistacia vera L.) for preservation in cold storage

Oxidative stress occurs during storage, often leading to a decrease in fruit quality and subsequently reducing its marketability. In this investigation, the ability of glycine betaine (GB) to extend the quality and storage of fresh pistachios stored in cold conditions was evaluated. The independent variables in this study were the concentration of GB (0, 5, 10 and 15 mM) and storage period (at harvest, 25, 50, and 75 days after harvest). Over time, the chlorophyll and carotenoid content of both kernels and hulls, as well as phenolics, flavonoids, antioxidant activity, taste, aroma, appearance of shells and hulls, firmness, and overall weight, tend to decrease. However, glycine betaine (GB) significantly prevents these reductions. GB effectively countered the rise in malondialdehyde, ion leakage, and hydrogen peroxide levels in kernels over time. In GB-treated samples, anthocyanin levels initially increased when comparing harvest time in both kernels and hulls, but subsequently decreased. GB enhanced peroxidase activity and suppressed polyphenol oxidase activity. However, contrary to expectation, GB reduces phenylalanine ammonia lyase activity. Overall, higher GB concentrations lead to a more pronounced effect. Based on the results, GB, particularly at a concentration of 15 mM, is recommended for preserving fresh pistachios during cold storage.

FGF21 mediating the Sex-dependent Response to Dietary Macronutrients.

Sex is key variable influencing body composition and substrate utilization. At rest, females maintain greater adiposity than males and resist the mobilization of fat. Males maintain greater lean muscle mass and mobilize fat readily. Determining the mechanisms that direct these sex-dependent effects is important for both reproductive and metabolic health. Here, we highlight the fundamental importance of sex in shaping metabolic physiology and assess growing evidence that the hepatokine fibroblast growth factor-21 (FGF21) plays a mechanistic role to facilitate sex-dependent responses to a changing nutritional environment. First, we examine the importance of sex in modulating body composition and substrate utilization. We summarize new data that point toward sex-biased effects of pharmacologic FGF21 administration on these endpoints. When energy is not limited, metabolic responses to FGF21 mirror broader sex differences; FGF21-treated males conserve lean mass at the expense of increased lipid catabolism, whereas FGF21-treated females conserve fat mass at the expense of reduced lean mass. Next, we examine the importance of sex in modulating the endogenous secretion of FGF21 in response to changing macronutrient and energy availability. During the resting state when energy is not limited, macronutrient imbalance increases the secretion of FGF21 more so in males than females. When energy is limited, the effect of sex on both the secretion of FGF21 and its metabolic actions may be reversed. Altogether, we argue that a growing literature supports FGF21 as a plausible mechanism contributing to the sex-dependent mobilization vs preservation of lipid storage and highlight the need for further research.

Active Discovery of the Allosteric Inhibitor Targeting Botrytis cinerea Chitinase Based on Neural Relational Inference for Food Preservation.

Currently, allosteric inhibitors have emerged as an effective strategy in the development of preservatives against the drug-resistant Botrytis cinerea (B. cinerea). However, their passively driven development efficiency has proven challenging to meet the practical demands. Here, leveraging the deep learning Neural Relational Inference (NRI) framework, we actively identified an allosteric inhibitor targeting B. cinerea Chitinase, namely, 2-acetonaphthone. 2-Acetonaphthone binds to the crucial domain of Chitinase, forming the strong interaction with the allosteric sites. Throughout the interaction process, 2-acetonaphthone diminished the overall connectivity of the protein, inducing conformational changes. These findings align with the results obtained from Chitinase activity experiments, revealing an IC50 value of 67.6 μg/mL. Moreover, 2-acetonaphthone exhibited outstanding anti-B. cinerea activity by inhibiting Chitinase. In the gray mold infection model, 2-acetonaphthone significantly extended the preservation time of cherry tomatoes, positioning it as a promising preservative for fruit storage.

Spraying zinc oxide nanoparticles on pistachios (Pistacia vera L.) for preservation in cold storage

ABSTRACT The ability of zinc oxide nanoparticles to extend the shelf life of fresh pistachios in polyethylene packages in cold storage was evaluated. Independent variables were the type and concentration of the disinfectant (0.5%, 1%, 1.5%, and 2% nano-ZnO and 2% peracetic acid) and storage time (at harvest, 25, 50 and 75 days after harvest). Aflatoxin was not detected in the kernels of the disinfectant treatments after storage. A decrease occurred in firmness, chlorophylls and carotenoids in hulls and kernels over time. Phenolics and flavonoids of kernels decreased, parallel to a decrease in weight. Nano-ZnO (2%) prevented this reduction well. In hulls and kernels, nano-ZnO (2%) revealed the highest anthocyanin. Antioxidant activity in kernels remained stable or even increased in response to ZnO (1.5%) on day 75. All ZnO treatments prevented ion leakage while limiting hydrogen peroxide and malondialdehyde production in hulls. Peracetic acid reduced polyphenol oxidase activity on 75th day. ZnO (1% and 1.5%) and peracetic acid increased phenylalanine ammonia lyase activity. On 75th day, ZnO-treated samples had higher peroxidase activity. All ZnO treatments, especially 1.5% and 2%, prevented the loss of quality in hulls, shells, taste, and aroma. Nano-ZnO (2%) can be recommended for fresh pistachio preservation in cold storage.

Reversible Nucleic Acid Storage in Deconstructable Glassy Polymer Networks.

The rapid decline in DNA sequencing costs has fueled the demand for nucleic acid collection to unravel genomic information, develop treatments for genetic diseases, and track emerging biological threats. Current approaches to maintaining these nucleic acid collections hinge on continuous electricity for maintaining low-temperature and intricate cold-chain logistics. Inspired by the millennia-long preservation of fossilized biological specimens in calcified minerals or glassy amber, we present Thermoset-REinforced Xeropreservation (T-REX): a method for storing DNA in deconstructable glassy polymer networks. Key to T-REX is the development of polyplexes for nucleic acid encapsulation, streamlining the transfer of DNA from aqueous to organic phases, replete with initiators, monomers, cross-linkers, and thionolactone-based cleavable comonomers required to form the polymer networks. This process successfully encapsulates DNA that spans different length scales, from tens of bases to gigabases, in a matter of hours compared to days with traditional silica-based encapsulation. Further, T-REX permits the extraction of DNA using comparatively benign reagents, unlike the hazardous hydrofluoric acid required for recovery from silica. T-REX provides a path toward low-cost, time-efficient, and long-term nucleic acid preservation for synthetic biology, genomics, and digital information storage, potentially overcoming traditional low-temperature storage challenges.

Quantifying the teleconnections of carbon storage in construction land and its socioeconomic driving forces in Guangdong Province

The development of regional trade has fostered teleconnections in land resource across various regions, emerging as a pivotal influence on the expansion of construction land. However, the potential consequences of regional trade on carbon storage associated with construction land remain largely unquantified. Therefore, this study zeroes in on Guangdong Province as the focal point, employing China's multiregional input-output model to quantify interregional teleconnections and delineate changes in carbon storage. Through meticulous analysis, it seeks to pinpoint the catalysts driving these alterations in teleconnections. By delving into the latest teleconnections within Guangdong, the study unveils the ramifications of the province's trade scale and structure with other provinces on carbon storage, thereby identifying imperative avenues for carbon storage preservation. The findings underscore that during the study period, the expansion of construction land in Guangdong Province precipitated a notable loss of land carbon storage, amounting to 2.6 Tg C, primarily sourced from forests and arable land. Concurrently, the total carbon flow surged by 20.77 Tg C, propelled by a 4.63 Tg C influx and a 16.05 Tg C outflow, resulting in a net inflow decrease of 11.51 Tg C. This delineates the influence of external forces on carbon storage within Guangdong Province, with the surge primarily attributed to heightened external demand, notably for construction land. Nevertheless, technological advancements have partially alleviated the impact of this demand surge on carbon storage. The outcomes of this study underscore the pivotal role of external linkages in shaping carbon storage dynamics and furnish invaluable insights for steering future regional endeavors towards carbon neutrality objectives.

Hsp70 Knockdown in the Brine Shrimp Artemia franciscana: Implication on Reproduction, Immune Response and Embryonic Cuticular Structure.

The potential functional role(s) of heat shock protein 70 (Hsp70) in the brine shrimp, Artemia franciscana, a crucial crustacean species for aquaculture and stress response studies, was investigated in this study. Though we have previously reported that Hsp70 knockdown may have little or no impact on Artemia development, the gestational survival and number of offspring released by adult females were impaired by obscuring Hsp70 synthesis. Transcriptomic analysis revealed that several cuticle and chitin synthetic genes were downregulated, and carbohydrate metabolic genes were differentially expressed in Hsp70-knockdown individuals. A more comprehensive microscopic examination performed in this study revealed exoskeleton structural destruction and abnormal eye lenses featured in Hsp70-deficient adult females 48h after Hsp70 dsRNA injection. Cysts produced by these Hsp70-deficient broods, instead, had a defective shell and were smaller in size, whereas nauplii had shorter first antennae and a rougher body epicuticle surface. Changes in carbohydrate metabolism caused by Hsp70 knockdown affected glycogen levels in adult Artemia females, as well as trehalose in cysts released from these broods, indicating that Hsp70 may play a role in energy storage preservation. Outcomes from this work provided novel insights into the roles of Hsp70 in Artemia reproduction performance, cyst formation, and exoskeleton structure preservation. The findings also support our previous observation that Hsp70 knockdown reduced Artemia nauplius tolerance to bacterial pathogens, which could be explained by the fact that loss of Hsp70 downregulated several Toll receptor genes (NT1 and Spaetzle) and reduced the integrity of the exoskeleton, allowing pathogens to enter and cause infection, ultimately resulting in mortality.

High performance poly(L-lactic acid)-based film by one-step synthesis of poly (L-lactic acid-co-butylene itaconate-co-glycolic acid) for efficient preservation of yogurt storage

Biodegradable poly(L-lactic acid) (PLLA) has seldom used for dairy packaging due to medium permeability and brittleness. Novel PLLA copolymers, poly (L-lactic acid-co-butylene itaconate-co-glycolic acid) (PLBIGA), were developed by integrating glycolic acid (GA) and poly(butylene itaconate) (PBI) into PLLA's structure using low molecular weight PLLA as a key initiator. Then, packaging materials with better barrier and mechanical properties were obtained by blended PLBIGA with PLLA. Both PLLA/PLBIGA films and polyethylene nylon composite film (PE/NY) were used for stirred yogurt packaging and storage at 4 °C for 25 days. Results revealed that yogurt packed by PLLA/PLBIGA films maintained stabler water-holding capacity, color, and viscosity over the storage period. Moreover, the integrity of the gel structure and the total viable count of lactic acid bacteria in yogurt packaged in PLLA/40-PLBIGA8 were also found to be superior to those in PE/NY packages, highlighting its eco-friendly advantages in dairy packaging.

Blockchain-enabled infrastructural security solution for serverless consortium fog and edge computing.

The robust development of the blockchain distributed ledger, the Internet of Things (IoT), and fog computing-enabled connected devices and nodes has changed our lifestyle nowadays. Due to this, the increased rate of device sales and utilization increases the demand for edge computing technology with collaborative procedures. However, there is a well-established paradigm designed to optimize various distinct quality-of-service requirements, including bandwidth, latency, transmission power, delay, duty cycle, throughput, response, and edge sense, and bring computation and data storage closer to the devices and edges, along with ledger security and privacy during transmission. In this article, we present a systematic review of blockchain Hyperledger enabling fog and edge computing, which integrates as an outsourcing computation over the serverless consortium network environment. The main objective of this article is to classify recently published articles and survey reports on the current status in the domain of edge distributed computing and outsourcing computation, such as fog and edge. In addition, we proposed a blockchain-Hyperledger Sawtooth-enabled serverless edge-based distributed outsourcing computation architecture. This theoretical architecture-based solution delivers robust data security in terms of integrity, transparency, provenance, and privacy-protected preservation in the immutable storage to store the outsourcing computational ledgers. This article also highlights the changes between the proposed taxonomy and the current system based on distinct parameters, such as system security and privacy. Finally, a few open research issues and limitations with promising future directions are listed for future research work.

The impact of preservation solutions for static cold storage on kidney transplantation outcomes: Results of a Brazilian nationwide multicenter study.

This study evaluated the current practices of selecting cold storage preservation solutions in Brazil and their impact on delayed graft function (DGF) incidence and 1-year outcomes in kidney transplant recipients. A retrospective cohort study was conducted, including 3,134 brain-dead deceased donor kidney transplants performed between 2014 and 2015 in 18 Brazilian centers. The most commonly used preservation solution was Euro-collins (EC, 55.4%), followed by Histidine-tryptophan-ketoglutarate (HTK, 30%) and Institut Georges Lopez (IGL-1, 14.6%). The incidence of DGF was 54.4%, with 11.7% of patients requiring dialysis for more than 14 days, indicating prolonged DGF. Upon adjusting for confounding variables, HTK demonstrated a significantly lower risk of DGF than EC (OR 0.7350.82500.926), as did IGL-1 (OR 0.6050.7120.837). Similar protective effects were observed for prolonged DGF when comparing HTK (OR 0.4780.5990.749) and IGL-1 (OR 0.4780.6810.749) against EC. No significant association was found between preservation solutions and 1-year death-censored graft survival. In conclusion, EC was the most frequently used cold storage perfusion solution, demonstrating a higher incidence and duration of DGF compared with HTK and IGL-1, but with no impact on 1-year graft survival.

Effect of atmospheric cold plasma treatment modes on the quality of red shrimp (Solenocera crassicornis) during cold chain storage

An increasingly popular non-thermal approach for food preservation is atmospheric cold plasma (ACP), which can efficiently inactivate various microorganisms. This study explored the impact of different ACP treatment modes on the preservation of red shrimp in cold chain storage. A novel treatment mode, ACP cyclical, was introduced as an alternative to ACP one-time treatment. The results indicated that the ACP cyclical treatment extended the ozone and shrimp exposure time to 35 min compared to one-time treatment, resulting in a notable enhancement in preservation effectiveness. While ACP treatment significantly reduced the total viable count (TVC) of red shrimp at day 0, it does not affect total volatile basic-nitrogen (TVB-N), pH value, or color. At day 8, the ACP cyclical group showed a 17.38% reduction in TVB-N, 36.90% decrease in TVC, and 32.39% decline in sulfur-producing bacteria compared to the ACP one-time treatment group. Electronic nose analysis indicated improved odor stability in both ACP treatment modes. Moreover, correlation and partial least squares discriminant analysis revealed that ACP cyclical treatment delayed shrimp’ color deterioration and TVB-N increase during prolonged storage. This study highlighted the potential of ACP cyclical treatment for enhancing red shrimp preservation in cold chain storage.

The Technology in Cryotechnology

The process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or assisted fertilization. In this work we provide an overview of various technologies in the field of cryotechnology with regard to the freezing, storage and thawing of living cells. The first part covers the freezing process, starting with cryoprotective agents regarding their protection mechanisms and compositions, passing by cryoimaging, micro-fluidic systems, and the currently available freezing and biobanking equipment. The second part focusses on the thawing process as well as the hypothermic preservation for the short-term storage of biological materials and constructs.

Hypothermic oxygenated perfusion (HOPE) safely and effectively extends acceptable donor heart preservation times: Results of the Australian and New Zealand trial

BackgroundCold static storage (CSS) preservation of donor hearts for periods longer than 4 hours increases the risk of primary graft dysfunction (PGD). The aim of the study was to determine if hypothermic oxygenated perfusion (HOPE) could safely prolong the preservation time of donor hearts. MethodsWe conducted a non-randomised, single arm, multi-center investigation of the effect of HOPE using the XVIVO Heart Preservation System on donor hearts with a projected preservation time of 6-8 hours on 30-day recipient survival and allograft function post-transplant. Each center completed 1 or 2 short preservation time followed by long preservation time cases. PGD was classified as occurring in the first 24 hours after transplantation or secondary (SGD) occurring at any time with a clearly defined cause. Trial survival was compared with a comparator group based on data from the International Society of Heart and Lung Transplantation Registry. ResultsWe performed heart transplants using 7 short and 29 long preservation time donor hearts placed on the HOPE system. The mean preservation time for the long preservation time cases was 414 minutes, the longest being 8 hours and 47 minutes. There was 100% survival at 30 days. One long preservation time recipient developed PGD, and 1 developed SGD. One short preservation time patient developed SGD. 30-day survival was superior to the ISHLT comparator group despite substantially longer preservation times in the trial patients. ConclusionsHOPE provides effective preservation out to preservation times of nearly 9 hours allowing retrieval from remote geographic locations.

Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability

Intestinal transplantation (IT) is the final treatment option for intestinal failure. Static cold storage (CS) is the standard preservation method used for intestinal allografts. However, CS and subsequent transplantation induce ischemia-reperfusion injury (IRI). Severe IRI impairs epithelial barrier function, including loss of intestinal stem cells (ISC), critical to epithelial regeneration. Normothermic machine perfusion (NMP) preservation of kidney and liver allografts minimizes CS-associated IRI; however, it has not been used clinically for IT. We hypothesized that intestine NMP would induce less epithelial injury and better protect the intestine’s regenerative ability when compared with CS. Full-length porcine jejunum and ileum were procured, stored at 4 °C, or perfused at 34 °C for 6 hours (T6), and transplanted. Histology was assessed following procurement (T0), T6, and 1 hour after reperfusion. Real-time quantitative reverse transcription polymerase chain reaction, immunofluorescence, and crypt culture measured ISC viability and proliferative potential. A greater number of NMP-preserved intestine recipients survived posttransplant, which correlated with significantly decreased tissue injury following 1-hour reperfusion in NMP compared with CS samples. Additionally, ISC gene expression, spheroid area, and cellular proliferation were significantly increased in NMP-T6 compared with CS-T6 intestine. NMP appears to reduce IRI and improve graft regeneration with improved ISC viability and proliferation.

Quasi‐identifier recognition with echo chamber optimization‐based anonymization for privacy preservation of cloud storage

SummaryThe publication of data raises several security concerns, implying that when a reputable company offers data to a third party, personal information is not needed to be revealed. The fundamental issue that led to identity leakage is the connection of quasi‐identifiers (QIDs); however, the majority of researchers overlook the identification of accurate QIDs. This study developed a privacy preservation and clustering‐based quasi‐identification model based on the Echo Chamber optimization with a Z‐mixture parameter for privacy‐preserving combined data publishing to maintain the privacy of the data. The Echo chamber optimization is used to conduct a clustering‐based quasi‐identification to find the significant attributes especially, in determining the dimension of the solutions as well as the precise QIDs using the re‐identification risk rate as the fitness function. The loss of information is greatly reduced in terms of the metrics such as normalized certainty penalty metric, average equivalent class size metric, and discernibility metric. The developed optimized clustering‐based algorithm with the privacy preservation model extensively minimizes the leakage of private information and the utilization of data is well‐maintained compared with other existing algorithms.

Development of a Chicken Gastrointestinal Tract (GIT) Simulation Model: Impact of Cecal Inoculum Storage Preservation Conditions

A chicken gastrointestinal tract (GIT) simulation model was developed to help predict the potential effects of feed additives supplementation on chicken’ microbiota. The chemical and enzymatic conditions for oral, gastric, intestinal, and cecum fermentation phases were designed to closely resemble the chicken GIT conditions. For cecum fermentation, the inoculum was obtained from the cecal contents of 18 38-day broiler chickens. The impact of inoculum preservation on bacteria viability was assessed by comparing two methods of preservation with fresh inoculum: (1) 5% dimethyl sulfoxide (DMSO) at −80 °C and (2) 30% glycerol at −20 °C. The fermentation with fresh and frozen (DMSO method) inoculums was performed and compared using standard chicken feed (SCF) and SCF with 1% fructooligosaccharides (FOS), and inoculum control (IC) condition without feed matrix was used as a baseline. Inoculum’s viability was assessed throughout 90 days of storage by culture media platting, while bacterial growth and metabolites production during fermentation was evaluated by quantitative polymerase chain reaction (qPCR), high-performance liquid chromatography (HPLC), and total ammonia nitrogen quantification. The DMSO method was shown to be the most suitable for cecal inoculum storage. Higher growth of beneficial cecal bacteria for fresh inoculum was observed in SCF while for frozen inoculum, was the SCF + FOS condition. Also, frozen inoculum had lower activity of butyrate producers and proteolytic bacteria, showing different fermentation profiles. The GIT model developed showed to be useful to test the effect of feed additives supplementation.

European Society for Organ Transplantation (ESOT) Consensus Statement on the Role of Pancreas Machine Perfusion to Increase the Donor Pool for Beta Cell Replacement Therapy.

The advent of Machine Perfusion (MP) as a superior form of preservation and assessment for cold storage of both high-risk kidney's and the liver presents opportunities in the field of beta-cell replacement. It is yet unknown whether such techniques, when applied to the pancreas, can increase the pool of suitable donor organs as well as ameliorating the effects of ischemia incurred during the retrieval process. Recent experimental models of pancreatic MP appear promising. Applications of MP to the pancreas, needs refinement regarding perfusion protocols and organ viability assessment criteria. To address the "Role of pancreas machine perfusion to increase the donor pool for beta cell replacement," the European Society for Organ Transplantation (ESOT) assembled a dedicated working group comprising of experts to review literature pertaining to the role of MP as a method of improving donor pancreas quality as well as quantity available for transplant, and to develop guidelines founded on evidence-based reviews in experimental and clinical settings. These were subsequently refined during the Consensus Conference when this took place in Prague.