Several cultural and historical objects are made up of wood and leather that are prone to the attack of wood- and leather-degrading microorganisms. Lignolytic fungi have a huge potential to degrade such historical objects that are made up of wood and leather. To protect these types of objects from lignolytic fungi, several chemical agents are being used which have a significant impact on the environment that necessitates sustainable approaches to control these microorganisms. In the present study, seven lignolytic fungi (four from wood and three from leather) were isolated and were identified as Colletotrichum capsici, Phoma spp., Aspergillus niger, Fusarium oxysporum, Aspergillus flavus, Aspergillus paraciticus and Colletotrichum capsici-1. All these fungi were able to produce a significant quantity of lignin peroxidase enzyme which confirms that these do have the potential to degrade wood and leather objects. 1200 mg/L was found to be the minimum inhibitory concentration for Aspergillus flavus, Colletotrichum capsici, Fusarium oxysporum, Colletotrichum capsici-1 and Aspergillus parasiticus. However, growth of Aspergillus niger and Phoma spp. was invisible at a concentration of 1400 mg/L, which is the minimum inhibitory concentration of Eucalyptus oil for Aspergillus niger and Phoma spp. At a concentration of 1200 mg/L of Eucalyptus oil, growth of Aspergillus flavus, Colletotrichum capsici-1, Fusarium oxysporum, Colletotrichum capsici and Aspergillus parasiticus was completely inhibited, while 100% growth inhibition was found for Aspergillus niger and Phoma spp. at 1400 mg/L of Eucalyptus oil. This study revealed that these lignolytic fungi may causes degradation of wood and leather made historical objects due to the presence of several organic compounds in Eucalyptus oil that have high anti-fungal activity. It was found that Eucalyptus oil has huge potential to control growth of lignolytic fungi including C. capsici, Phoma spp., A. niger, F. oxysporum, A. flavus, A. paraciticus and C. capsici-1 to protect wood and leather made historical objects in a sustainable and environmentally friendly way.