Presence Of Phosphate Buffer Solution Research Articles

Effects of the presence of phosphate buffer solution on removal efficiency of Pb and Zn in soil by solid phase microbial fuel cells.

Simple, effective and environment-friendly ways for remediating toxic metal pollution are necessary. In this study, the effect of different concentrations phosphate buffer solution (PBS) on removal efficiency of Pb and Zn in soil by solid phase microbial fuel cell (SMFC) was investigated. During 100days of operation, the SMFC with 150mM PBS generated the highest power density of 21.7 mW m-2 and the lowest internal resistance of 161 Ω. The addition of PBS can also increase soil conductivity and maintain a suitable pH for microbial activity. Furthermore, the removal rate of Pb and Zn in the SMFC with 150mM PBS can reach 14.7% and 22.3%, respectively. The microbial community analyses demonstrated that Anditalea as an exoelectrogen in alkaline-saline conditions was significantly enriched in the SMFC with 150mM PBS. This study provides an effective strategy for strengthening SMFC to remove toxic metals in soil.

Li2TiO3-MWCNT nanocomposite electrodes for determination of dopamine in electrochemical sensing platform

The Li2TiO3-multiwalled carbon nanotube nanocomposite (LTO-MWCNT) prepared by a one-step hydrothermal route is investigated as an electrochemical sensor for pharmaceutical formulations. X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy and transmission electron microscopy show that MWCNTs are wrapped in well-crystallized LTO cubical-shaped nanoparticles (monoclinic structure, C2/c space group, 53 nm in size) forming an electrode material with a well-connected conductive network. BET analyses evidence the high specific surface area (SSA) of 32 m2 g−1 with an average pore diameter of 16 nm for LTO-MWCNT nanocomposite, which offer numerous electroactive sites, while the pore diameter prevents structural instability resulting in exemplary electrochemical performance. The electrochemical properties of a glassy carbon (GC) electrode modified by the composite LTO-MWCNT are carried out by cyclic voltammetry and differential pulse voltammetry for the test of electrocatalytic activity of dopamine (DA) in the presence of phosphate buffer solution (PBS) as an electrolyte at pH 6.5. The LTO-MWCNT/GC electrode thus demonstrates excellent sensitivity and selectivity for acetaminophen (AC) and tyrosine (TY) sensing. The limit of detection (LOD) and the limit of quantification (LOQ) are found to be 1.71 and 5.7 μmol L−1, respectively, with the dynamic range 5.0–1000 μmol L−1. The newly fabricated LTO-MWCNT/GC sensor shows good accuracy and precision with satisfactory recoveries of 98% in dopamine injection formulation. These results suggest that the LTO-MWCNT/GC sensor has great potential for successful application towards the determination of DA and pharmaceutical formulations.

Electro-sensing base for hazardous pesticide 2, 4-DCP and its quantification in real samples at ZnO@Cu core-shell nanoparticles in the presence of cationic surfactant

We developed a novel sensor using carbon paste electrode (CPE) modified with cationic surfactant cetyltrimethylammonium bromide (CTAB) and the core-shell nanoparticles (NPs) of zinc oxide at copper (ZnO@Cu) to study an electrochemical behavior of 2, 4 - dichlorophenol (2, 4 - DCP), a harmful molecule. The core-shell ZnO@Cu NPs/CTAB/CPE is proved to be sensitive because of its sensing features for 2, 4-DCP. The sensor showed excellent electro-catalytic behavior and elevation in peak current in the presence of phosphate buffer solution (PBS) of 0.2 M strength. Advanced techniques like transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDS), and scanning electron microscope (SEM) were employed to ZnO@Cu core-shell NPs characterization. The cyclic voltammetry (CV), as well as square wave voltammetry (SWV) techniques, were applied to study electro-kinetic parameters, including specific heterogeneous rate constant, charge transfer coefficient, accumulation time, scan rate, the effect of temperature, pH, the energy of activation, enthalpy, entropy, Gibb's free energy, and the number of electrons transferred in the 2, 4 - DCP electro-oxidation. By SWV technique, the 2, 4 - DCP molecule was analyzed in vegetables, fruits, water, and soil samples.

Ultra-high sensitive, selective, non-enzymatic dopamine sensor based on electrochemically active graphene decorated Polydiphenylamine-SiO2 nanohybrid composite

In neurotransmission process, dopamine (DA) is the key molecule. Hence, the detection of dopamine plays a vital role in the primary diagnosis of diseases which is related to the irregular levels of dopamine. In this study, we proposed the electrochemically deposited Graphene Nanosheets-Polydiphenylamine-Silica (GRNS-PDPA-SiO2) modified nanohybrid electrode for an amperometric detection of dopamine. The electrochemical oxidation of DA ensures the catalytic activity of the prepared nanohybrid composites by chronoamperometry (CA) method. The nanohybrid electrode shows linear response towards DA (1–5 μM) in the presence of phosphate buffer solution (PBS at pH 7.0) with the regression co-efficient (R2) of 0.98. Low detection limit with superior sensitivity of dopamine was achieved as 0.1 μM and 0.66 μA μM−1cm−2 respectively. The structural morphology and the chemical homogeneity of the materials were confirmed by scanning electron microscope with EDX analysis. The molecular vibration and electrochemical performances were recorded through Fourier-transform infrared spectroscopy (FTIR) and cyclic voltammetry (CV). The adopted GRNS-PDPA-SiO2 modified electrode resulted in excellent sensitivity, high selectivity claims its realistic applications in the highly clinical diagnostics.

The effect of PBS on methane production in combined MEC-AD system fed with alkaline pretreated sewage sludge

Batch experiments were conducted to study bioelectrochemical enhancement of the anaerobic digestion (AD) of alkaline-pretreated sewage sludge in microbial electrolysis cell (MEC) in the presence or absence of phosphate buffer solution (PBS). Experimental results showed that the maximum methane production rate was increased from 0.18 mL-CH4/(mLreactor·d) (control) to 0.2 mL-CH4/(mLreactor·d) in the presence of PBS and cumulative methane production was 1.4-fold higher than that of control. The initial concentrations of SCOD, protein, polysaccharide and VFAs in the presence of PBS were slightly higher than those of control, suggesting that PBS might facilitate the release of organics into mixed liquor. The microbial community analysis results showed that the microbial community with PBS had higher diversity and Bacteroidetes and Firmicutes were the two most abundant phyla in the communities. Moreover, the PBS addition could enhance the growth of aceticlastic methanogens (Methanosaeta) and inhibit a portion of hydrogenotrophic methanogens (Methanobacterium), possibly due to the different cell wall composition.

A polydopamine-mediated biomimetic facile synthesis of molybdenum carbide-phosphide nanodots encapsulated in carbon shell for electrochemical hydrogen evolution reaction with long-term durability

Biomimetic environmental benign synthesis of electrocatalysts with inexpensive transition metal compounds is a promising approach to achieve clean hydrogen production with high sustainability via water splitting. Herein, a mussel-inspired facile synthesis of N-carbon encapsulated molybdenum carbide-phosphide (Mo2C–MoP) hybrid nanodots using a polydopamine biopolymer for efficient hydrogen evolution reactions (HERs), and long-term durability is demonstrated. The unique properties of polydopamine facilitate the transition metal ions (i.e., molybdenum) chelation without the use of additional toxic chelating agents. In the presence of phosphate buffer solution, the anchoring properties of polydopamine induce the formation of molybdenum-based complexes with the carbon and phosphide species, resulting in the synthesis of Mo2C–MoP hybrid nanodots encapsulated in nitrogen-doped carbon shell. Consequently, the Mo2C–MoP hybrid nanodots exhibit considerable electrocatalytic HER performance and long-term stability. The low overpotential of 147 mV is obtained at the high current density of 20 mA cm−2 along with the low Tafel value of 64.92 mV dec−1 in alkaline medium. Furthermore, the long-term stability over 120 h is achieved using Mo2C–MoP@CP. Therefore, the use of an environment-friendly biopolymer for the fabrication of noble-metal-free, efficient, and stable HER electrocatalysts can be a promising strategy to achieve sustainable and clean H2 production.

A facile synthesis of Fe3O4-Gr nanocomposite and its effective use as electrochemical sensor for the determination of dopamine and as anode material in lithium ion batteries

The Fe3O4-Graphene (Fe3O4-Gr) nanocomposite has been synthesized by hydrothermal method and demonstrated its application towards electrochemical sensor and anode for high performance lithium ion batteries. The nanocomposite exhibited cubic face centred Fe3O4 structure with an estimated crystallite size of 25 nm. The additional peak at 2θ = 26.4° from XRD spectrum confirmed the inculcation of graphene in the composite. The embedment of Fe3O4 nanoparticles in graphene sheets was further confirmed from TEM analysis. The FESEM analysis revealed that the cubical shaped Fe3O4 particles were strongly anchored with graphene nanosheets with an estimated grain size of 40 nm. The electrochemical properties of Fe3O4-Gr were studied with the help of cyclic voltammetry (CV), Differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and chronopotentiometry (CP). The sensor performance was studied by testing the electrochemical activity of dopamine (DA) in presence of phosphate buffer solution (PBS) as an electrolyte at pH 6 using CV and DPV. The Fe3O4-Gr/Glassy carbon electrode (GCE) sensor showed ultimate sensitivity and selectivity in the presence of uric acid (UA) and acetaminophen (AC). It demonstrated utmost low limit of detection of 0.78 μM towards the detection of DA and satisfactory recoveries in dopamine injection formulation. Moreover, as an anode material for Li-ion batteries, the Fe3O4-Gr nanocomposite delivered high discharge capacity of 920 mA h g−1 and retained 96% capacity even after 30 discharge cycles.

Efficient electrochemical detection of guanine, uric acid and their mixture by composite of nano-particles of lanthanides ortho-ferrite XFeO3 (X = La, Gd, Pr, Dy, Sm, Ce and Tb)

Nanoparticles of lanthanides orthoferrites were found to be efficient electrodes for detection of guanine (GU) and uric acid (UA).These perovskite type lanthanide orthoferrite nano particles of XFeO3 (where X = La, Ce, Pr, Sm, Gd, Tb and Dy) were synthesized by combustion techniques using lanthanides nitrate and ferric nitrate as precursor materials in the presence of ethanolamine and sugar as fuel. The powders were characterized by FESEM, TEM and XRD. The synthesized nano particles have orthorhombic crystal structure and have average crystallite sizes ranging from 40 to 45 nm as calculated by Debye-Scherrer equation. The electrochemical activities of these oxides with graphite powder as sensor electrodes were studied for the detection of guanine and uric acid individually as well as in mixture by cyclic voltammetery (CV) and also by differential pulse voltammetry (DPV) techniques and found to be better than reported literatures. The efficient detection of bio-molecules may be due to the unique band gap of these ortho-ferrite semiconductors having the bad-gap value around 2.0 eV. The detection limit varies from 100 to 400 nM for guanine and 200 to 500 nM for uric acid at the surface of XFeO3 electrodes. The best electrode was obtained from LaFeO3 with lowest detection limit 100 nM for GU & 200 nM for UA maintaining the linear range from 0.5 μM to 120 μM. All electrochemical experiments have been carried out in the presence of Phosphate buffer solution of pH 5.0 as a supporting electrolyte with scan rate 100 mV s−1 and 50 mV s−1 for CV and DPV respectively.

Amperometry detection of nitrite in food samples using tetrasulfonated copper phthalocyanine modified glassy carbon electrode

A stable chitosan protected tetrasulfonated copper phthalocyanine (Chit-TsCuPc) modified glassy carbon electrode (GCE) was used to aid the electrochemical detection of nitrite (NO2−) in food samples. The electrochemical oxidation of NO2− was investigated on bare, chitosan and TsCuPc modified GCE in presence of phosphate buffer solution with an acidic medium. The elevated oxidation peak current was noticed at less oxidation potential for + 0.55 V vs. Ag/AgCl at Chit-TsCuPc modified electrode under the optimized experimental conditions. The plot displays the peak current against NO2− with a good linearity in the concentration rangefrom 6.5 × 10−8 M to 5.0 × 10−10M. The linear regression equation is calculated to be Ip (μA) = 0.5291 + 1.4894C (NO2−), has a good correlation coefficient of 0.9949 and the detection limit of 22 nM (S/N = 3) with the current sensitivity of 4.964 μA nM−1 cm−2. The standard addition method was adopted to measure the unknown NO2− concentration in food samples.

A Highly Efficient Nanomaterial with Molecular Imprinting Polymer: Carbon Nitride Nanotubes Decorated with Graphene Quantum Dots for Sensitive Electrochemical Determination of Chlorpyrifos

Chlorpyrifos (CHL) is organophosphate insecticide and has low water solubility (1.39 mg/L). CHL is known to produce toxic effects by inhibiting the acetylcholinesterase enzyme activity. Because of this, the important health problems occur worldwide. Hence, it is important to detect the concentration of CHL at the sensitive levels in environmental waters. In this study, a novel molecular imprinted voltammetric sensor based on carbon nitride nanotubes (C3N4 NTs) decorated with graphene quantum dots (GQDs) modified glassy carbon electrode (GCE) was developed for determination of CHL. The unique C3N4 NTs@GQDs nanohybrid was synthesized by hydrothermal treatment. CHL imprinted GCE based on C3N4 NTs@GQDs nanohybrid was prepared via electropolymerization process of 100 mM pyrrole as monomer in the presence of phosphate buffer solution (PBS) (pH 7.0) containing 25 mM CHL. The prepared nanomaterials were characterized using scanning electron microscope (SEM), transmission electron microscope (TEM), raman spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS). The linearity range and the detection limit of the method were calculated as 1.0 × 10−11–1.0 × 10−9 M and 2.0 × 10−12 M, respectively. The sensor was applied to wastewater samples with good selectivity and recovery. The stability and selectivity of the voltammetric sensor were also reported.

Preparation of alanine and tyrosine functionalized graphene oxide nanoflakes and their modified carbon paste electrodes for the determination of dopamine

Herein, established the synthesis of graphene oxide (GO) by Hummers Method with addition of KMnO4 followed by thermal heating at 80°C. The obtained GO was further functionalized by alanine and tyrosine. The prepared GO, alanine functionalized GO nanoflakes (AGONF) and tyrosine functionalized GO nanoflakes (TGONF) were characterized by spectroscopic technique using energy-dispersive spectroscopy (EDS), quantitatively by scanning electron microscopy (SEM) and structural studies along with interlayer distance verified through X-ray diffraction technique. Afterwards, the prepared AGONF and TGONF were used as the modifier for the carbon paste electrode (CPE). The electrochemical behavior of the AGONF and TGONF modified carbon paste electrodes (MCPEs) towards dopamine (DA) in phosphate buffer solution (PBS) were examined by cyclic voltammetric (CV) technique and the obtained consequences showed good electrocatalytic activity of MCPEs by increasing the redox peak current with a lower potential difference compared to the bare CPE (BCPE). The AGONF and TGONF MCPEs were further used for the optimization studies. From the pH studies, it was found that the equal number of proton and electron transfer reaction involved in both the modified electrodes. The scan rate studies demonstrate the adsorption controlled electrode process at AGONF MCPE and diffusion controlled at TGONF MCPE. The oxidation peak current increased linearly with two concentration interval of DA at a range of 2–7μM and 10–30μM in presence of PBS (pH 7.4) at MCPEs and the limit of detection (LOD) were found to be 0.84μM and 0.96μM for first interval DA concentration range (2–7μM) at AGONF and TGONF MCPE. The stability, repeatability and reproducibility of functionalized GO nanoflakes MCPEs at DA were studied and established excellent characteristics. The newly developed functionalized GO nanoflake electrodes were successfully tested in DA injection sample. Furthermore the functionalized GO and surfactant (Sodium Alpha Olefin Sulphonate (SAOS)) immobilized functionalized GO MCPEs were examined for simultaneous determination of DA and ascorbic acid (AA) by differential pulse voltammetric technique.

Graphene quantum dot modified glassy carbon electrode for the determination of doxorubicin hydrochloride in human plasma

Low toxic graphene quantum dot (GQD) was synthesized by pyrolyzing citric acid in alkaline solution and characterized by ultraviolet--visible (UV–vis) spectroscopy, X-ray diffraction (XRD), atomic force microscopy (AFM), spectrofluorimetery and dynamic light scattering (DLS) techniques. GQD was used for electrode modification and electro-oxidation of doxorubicin (DOX) at low potential. A substantial decrease in the overvoltage (−0.56V) of the DOX oxidation reaction (compared to ordinary electrodes) was observed using GQD as coating of glassy carbon electrode (GCE). Differential pulse voltammetry was used to evaluate the analytical performance of DOX in the presence of phosphate buffer solution (pH 4.0) and good limit of detection was obtained by the proposed sensor. Such ability of GQD to promote the DOX electron-transfer reaction suggests great promise for its application as an electrochemical sensor.

Kinetics and Mechanism of Electron Transfer to Manganese(VII) by Fluorene and Its Halogenated Derivatives in Neutral Organic Medium

The kinetics of oxidation of fluorene (Fl) and its halogenated derivatives, namely, 2,7-dichlorofluorene (Fl-Cl), 2,7-dibromofluorene (Fl-Br) and 2,7-diiodofluorene (Fl-I), by permanganate ion in neutral organic medium in the presence of phosphate buffer solution has been investigated at a constant temperature of 25°C. The progresses of the reactions were followed spectrophotometrically. The stoichiometry of the reactions was found to be 3:4 (fluorene: permanganate). The oxidation reactions exhibited a first order dependence in [permanganate] and less than unit order dependences with respect to fluorenes concentrations. Under comparable experimental conditions, the order of the oxidation rate of the fluorene derivatives was: Fl-Cl > Fl > Fl-I > Fl-Br. The final oxidation products of fluorene derivatives were identified by GC/MS, FT-IR and chemical tools as the corresponding ketones (9H-fluorenone derivatives). The proposed oxidation mechanism involves formation of 1:1 intermediate complexes between fluorene derivatives and permanganate ion in pre-equilibrium step. The rate-law expression was deduced. The reactions constants involved in the different steps of the mechanism were evaluated. The activation parameters associated with the second order rate constants were calculated and discussed.

A Molecular Imprinted Voltammetric Sensor Based on Carbon Nitride Nanotubes: Application to Determination of Melamine

Melamine (MEL) has high nitrogen rich content. Because of this situation, it has been illegally added to dairy products by producers to obtain a higher readout of apparent protein content. This has been a cause of serious diseases and many infants have been intoxicated because the addition of MEL into food products can cause death. In this report, a novel molecular imprinted voltammetric sensor based on carbon nitride nanotubes (C3N4 NTs) modified glassy carbon electrode (GCE) was presented for determination of MEL. The developed surfaces were characterized using scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). MEL imprinted GCE was prepared via electropolymerization process of 100 mM phenol as monomer in the presence of phosphate buffer solution (PBS) (pH 7.0) containing 25 nM MEL. The linearity range and the detection limit of the method were calculated as 1.0 × 10−10 – 5.0 × 10−9 M and 1.0 × 10−11 M, respectively. The voltammetric sensor was applied to milk samples with good selectivity and recovery. The stability and reproducibility of the voltammetric sensor were also reported.

New molecular imprinted voltammetric sensor for determination of ochratoxin A

In this report, a novel molecular imprinted voltammetric sensor based on silver nanoparticles (AgNPs) involved in a polyoxometalate (H3PW12O40, POM) functionalized reduced graphene oxide (rGO) modified glassy carbon electrode (GCE) was presented for determination of ochrattoxin A (OCH). The developed surfaces were characterized using scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) method. OCH imprinted GCE was prepared via electropolymerization process of 100mM phenol as monomer in the presence of phosphate buffer solution (pH6.0) containing 25mM OCH. The linearity range and the detection limit of the method were calculated as 5.0×10−11−1.5×10−9M and 1.6×10−11M, respectively. The voltammetric sensor was applied to grape juice and wine samples with good selectivity and recovery. The stability of the voltammetric sensor was also reported.

The Spectrophotometric Determination of Famotidine Drug via Coupling with Diazotized Metochlopramide Hydrochloride

A new, simple and sensitive spectrophotometric method was described for the determination of famotidine (FAM) as a pure material and in pharmaceutical formulation. This method was based on diazotization and coupling reaction between famotidine and diazotized solution of metochlopramide hydrochloride (DMPH) in the presence of phosphate buffer solution to give a compound of azo dye having orange color soluble in water with high absorptivity at a wave length of 478 nm. The data shows that FAM and DMPH combine in the molar ratio of 1:1 at PH 7.0 .The method obeys Beer's law over concentration range of 1-40 ?g.ml-1 of famotidine with a correlation coefficient of 0.9955 and a detection limit of 0.10 ?g.ml-1. The apparent molar absorptivity referred to famotidine has been found to be 2.0 x 104 L. mol-1cm. -1. The proposed method was applied successfully to the assay of famotidine in pharmaceutical preparation.

The Spectrophotometric Determination of Famotidine Drug via Coupling with Diazotized Metochlopramide Hydrochloride

A new, simple and sensitive spectrophotometric method was described for the determination of famotidine (FAM) as a pure material and in pharmaceutical formulation. This method was based on diazotization and coupling reaction between famotidine and diazotized solution of metochlopramide hydrochloride (DMPH) in the presence of phosphate buffer solution to give a compound of azo dye having orange color soluble in water with high absorptivity at a wave length of 478 nm. The data shows that FAM and DMPH combine in the molar ratio of 1:1 at PH 7.0 .The method obeys Beer's law over concentration range of 1-40 ?g.ml-1 of famotidine with a correlation coefficient of 0.9955 and a detection limit of 0.10 ?g.ml-1. The apparent molar absorptivity referred to famotidine has been found to be 2.0 x 104 L. mol-1cm. -1. The proposed method was applied successfully to the assay of famotidine in pharmaceutical preparation.

Sensitive determination of citrinin based on molecular imprinted electrochemical sensor

In this report, a novel molecular imprinted voltammetric sensor based on glassy carbon electrode (GCE) modified with platinum nanoparticles (PtNPs) involved in a polyoxometalate (H3PW12O40, POM) functionalized reduced graphene oxide (rGO) was prepared for the determination of citrinin (CIT). The developed surfaces were characterized by using scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) method. CIT imprinted GCE was prepared via electropolymerization process of 80.0mM pyrrole as monomer in the presence of phosphate buffer solution (pH 6.0) containing 20.0mM CIT. The linearity range and the detection limit of the developed method were calculated as 1.0×10−12–1.0×10−10M and 2.0×10−13M, respectively. In addition, the voltammetric sensor was applied to rye samples. The stability and selectivity of the voltammetric sensor were also reported.

Electropolymerization of taurine on gold surface and its sensory application for determination of captopril in undiluted human serum

Polytaurine film was electrodeposited on gold (Au) electrode through cyclic voltammetry from taurine and phosphate buffer solution. The electrocatalytic effect of polytaurine modified Au (PT/Au) electrode was investigated for electro-oxidation of captopril (CAP). Electrocatalytical activity of PT/Au electrode was studied using cyclic voltammetry (CV), chronoamperometry and differential pulse voltammetry (DPV). DPV was used to evaluate the analytical performance of CAP in the presence of phosphate buffer solution and good limit of detection was obtained by this sensor. The experimental conditions influencing the determination of CAP were optimized and under optimal conditions, the oxidation peak current was proportional to CAP concentration in the range of 0.06–0.2μM, while the detection limit was 0.03μM (S/N=3). The results revealed that PT promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the method to direct assay of human serum is described. The proposed sensor was successfully applied to determine cadaverine in fish samples, yielding satisfactory results. The spiked recoveries were in the range of 96.0–105.0%.

Studies on the complexes of dialkyltin(IV) with dithizone

Abstract The absorption spectra of the dialkyltin(IV)-dithizone complexes extracted into carbon tetrachloride from aqueous solution are different with buffer solution used and pH in aqueous solution. Alkyl groups combining with tin have very little effect upon these changes of absorption spectra. By the method of the molar ratios, it is found that the 1:2 complex of dialkyltin(IV) with dithizone is formed in the presence of phosphate buffer solution, but the mixture of the 1:1 and the 1:2 complexes in acetate and formate buffers, and the 1:1 complex in monochloroacetate buffer. The anion of each buffer component, formate, acetate and monochloroacetate anions, coordinates with tin when the 1:1 complex of dialkyltin(IV) with dithizone is formed.