Presence Of Lupeol Research Articles

Lupeol a predisposing factor in grey mould (Amphobotrys ricini [N.F. Buchw.]) Hennebert disease pathogenesis in castor (Ricinus communis L.)

AbstractTo understand the castor wax layer's multifaceted contribution to Amphobotrys ricini pathogenesis, the hydrophobic components of cuticular wax were analysed from waxy and non‐waxy castor genotypes. Gas chromatography–mass spectrometry (GC–MS) technology enabled the stable detection and quantitative determination of various fatty acids and terpenoids. The investigation revealed a significant presence of triterpenoid compound ‘lupeol’, accounting for approximately 53.6% of the wax composition in the waxy genotype (DCH‐519), which was absent in non‐waxy genotype (ICS‐324). On exposure to lupeol, about 93.3% of conidia germinated leading to rapid mycelium growth and sporulation of A. ricini. SEM analysis of waxy and non‐waxy genotypes infected with A. ricini confirmed faster germination and production of longer germ tubes on waxy genotype compared with non‐waxy genotype, which may likely due to early recognition of the suitable host in the presence of lupeol, ultimately aiding in speedy germination and growth of the pathogen setting pace for pathogenesis.

Isolation, characterization, and encapsulation of a lupeol-rich fraction obtained from the hexanic extract of Coccoloba uvifera L. leaves

Aim: This study aimed to isolate, characterize, and encapsulate a lupeol-rich fraction obtained from the hexanic extract of Coccoloba uvifera L. leaves to evaluate its potential use in nutraceutical or pharmaceutical applications. Methods: The C. uvifera leaf extract was fractionated by column chromatography and the presence of lupeol was assessed by thin layer chromatography, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Additionally, the lupeol-rich fraction was characterized according to its antioxidant capacity and cytotoxicity. Finally, this fraction was encapsulated into electrospun nanofibers made of high degree of polymerization agave fructans (HDPAF) combined with polyethylene oxide (PEO). The obtained nanofibers were characterized in terms of morphology, chemical composition, and in vitro permeability using the Caco-2 cell line. Results: Fraction 6 showed a 77% of lupeol, quantified by chromatography, and presented a 7.3% inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH). 100 µg/mL of fraction 6 showed a decrease in Caco-2 cell viability. Finally, fraction 6 was encapsulated into electrospun nanofibers, which showed an increase in the apparent permeability of the lupeol present in fraction 6 in Caco-2 cells in comparison to neat fraction 6. Conclusions: It was possible to isolate and encapsulate a lupeol-rich fraction from C. uvifera into electrospun nanofibers, which allows the increasing the apparent permeability of lupeol, and consequently, they could be used for nutraceutical or pharmaceutical applications.

LUPEOL AND LAURIC ACID ISOLATED FROM ETHYL ACETATE STEM EXTRACT OF Justicia secunda AND THEIR ANTIMICROBIAL ACTIVITY

The method of cold maceration was used in the extraction of Justicia secunda starting with a non-polar (hexane) to a more polar solvent. The crude extracts of hexane, ethyl acetate, acetone, and methanol from the stem of were obtained using the polarity guided cold extraction method. Lupeol and Lauric acid are two well-known compounds that were discovered when a portion of the ethyl acetate extract of J. secunda was subjected to spectroscopic (1H NMR) structural elucidation. Based on the presence of Lupeol and Lauric acid in J. secunda stem, the plant could be a viable source of antimicrobial agents in the near future. The results of the antimicrobial activity obtained from the fractions of the Stem inhibited or exhibited activity against Methicillin resist Staph aureus, Vancomycin resistant enterococci, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Helicobacter pylori, Campylobacter fetus, Proteus mirabilis, Pseudomonas aeruginosa, Candida tropicalis, and Candida krusei. The plant J. secunda traditionally employed in the treatment of anemic circumstance, blood boost, wound healing, abdominal pain and fertility issues. The overall results confirm the significance of the use of the plant in traditional medicinal treatment of anamic circumstances, blood boost, wound healing etc, in line with reported claims because of the presence of isolated compounds.

Structural Identification of Chemical Constituents Isolated from Root Extracts of Jurinea Dolomiaea Boiss (Astraceae) by Chromatographic and Spectroscopic Methods

Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in Jurinea dolomiaea root extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography. Present research studies encompasses identification of chemical constituents in Jurinea dolomiaea roots of methanol extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents. The components were identified by matching mass spectra with MS libraries. There were 5 different compounds analyzed from Jurinea dolomiaea roots. The identified components are (2,4-ditert-butylphenyl)-5-hydroxypentanoate, 2‑ethylhexylheptadecyl sulphite, 6-methyltridecane, (9E, 12E)-9,12-Octadecadienoyl chloride, Linoleic acid chloride, linoleoyl chloride, Lup-20 (29)-en-3-ol Lup‑20(29)‑en‑3β‑ol Lup‑20(29)‑en‑3α‑ol Lupeol, (3α)‑isomer Lupeol, (3β,18β,19β)‑isomer, in Jurinea dolomiaea root extracts.

Osteogenic Activity of Lupeol Isolated from Clinacanthus nutans Lindau: Activity and Mode of Action

Clinacanthus nutans Lindau has been traditionally used for healing of bone fragility, but the mechanism of actions has not been clarified yet. In this study, the bone regeneration activity of lupeol derived from C. nutans was assessed using an in vitro model of osteoblast cells MC3T3-E1. The finding revealed that the compound was not significantly toxic to osteoblast cells at concentration of ≤40 μg/mL. Lupeol demonstrated the osteogenic activity through enhancement of alkaline phosphatase (ALP) of osteoblast cells up to 31.2%, 21%, and 12% at concentrations of 5, 10, and 20 µg/mL, respectively ( p < 0.05). Besides, the mineralization activity was increased up to 170, 230, 185, and 117% at concentration of 5, 10, 20, and 40 μg/mL, respectively ( p < 0.05). The marker genes related to osteoblast differentiation evaluated on the expression level in the presence of lupeol, including collagen I (col 1), osteopontin (opn), osterix (osx), and runx2, showed upregulated expression in all the test genes ( p < 0.05). The Western blot analysis demonstrated a clear effect of lupeol on expression of p38/p-p38, and ERK/p-ERK proteins involved in the MAPK signaling pathway. Thus, lupeol isolated from C. nutans exhibited the osteogenic activity by enhancing expression of important markers of osteogenesis, as well as affected the MAPK signaling pathway relating to osteoblast differentiation. This is the first report on the detailed mechanism of action of lupeol on bone regeneration and also explains for the traditional use of this medicinal plant for bone healing.

Isolation of Lupeol and Gallic acid with cytotoxic activity of two different extracts from the leaves of Iraqi Conocarpus erectus L.

Conocarpus erectus L. is a perennial, evergreen shrub belonging to Combretaceae family. Conocarpus plant reported to contain phenolic acid, flavonoids, lignan, terpenes and tannins. Aim of study was to isolate lupeol from hexane fraction and gallic acid from ethyl acetate fraction and investigate the effects of (hexane and ethyl acetate) fractions on viability of pancreatic AsPC-1 and breast MCF-7 cell lines by MTT assay. The presence of lupeol in the hexane and gallic acid in the ethyl acetate extracts was detected by TLC. The identification of isolated lupeol and gallic acid by HPTLC and HPLC comparing with standard lupeol and gallic acid. Structural elucidation of isolated compounds done by FTIR and UV spectrophotometer. The cytotoxic activity showed more at high concentration (30µg/ml) in both ethyl acetate and hexane fractions against MCF-7 cell line, the percentage of cellular inhibition for ethyl acetate at 30mg/ml was (73% and 79%) more than the hexane fraction in which the inhibition was (60% and 76%) at 48hr and 72 hr respectively. Furthermore, the cytotoxic activity more at high concentration (30µg/ml) in both fractions against AsPC-1 cell line with cellular inhibition (58% and 70%) for ethyl acetate fraction and (50% and 66%) for hexane fraction in compared with Cisplatin.

English

The proper use of plants and their extracts, whether for medicine or as a food additive, requires the use of extraction and characterization methods that allow the identification and quantification of the active principles in each extract, associating them with the effect produced. With this objective, the present work was developed to study the ultrasonic extracts of mangabeira (Hancornia speciosa Gomes), obtained from bark, leaves, and fruits of mangabeira in search of compounds with phytotherapeutic potential, particularly, anti-inflammatory activity. This plant is native to Brazil, belonging to the Apocinaceae family and is used in folk medicine to treat various diseases. The extracts were analyzed by gas chromatography and liquid chromatography, to test their antioxidant activity without toxicity. It was found that the extracts did not present toxicity as they have great antioxidant power. Identification of the compounds by GC/MS indicated the presence of Lupeol and Lupeol acetate as major compounds. The general characterization of extracts showed high contents of total phenolic compounds, flavonoids and flavonol in leaves and tannins (i€9 mg g-1) in the fruits. Some compounds such as Chlorogenic acid (i€ 35 mg g-1 in leaves and fruits), Catechin (i€ 35 mg g-1 in leaves, peels and fruits), Rutin (i€ 35 mg g-1 in leaves, peels and fruits) and Isoquercetin (35 mg g-1 only in the leaves) were also identified and quantified by HPLC/UV-DAD. Key words: Hancornia speciosa Gomes; medicinal plants; Gas chromatography; Lupeol; Antioxidants; flavonoids.

Composition of lipids from the First Lusatian lignite seam of the Konin Basin (Poland): Relationships with vegetation, climate and carbon cycling during the mid-Miocene Climatic Optimum

Samples of detrital lignite have been collected for detailed organic geochemical and carbon isotope analyses from the First Lusatian lignite seam at the Adamów, Jóźwin IIB and Tomisławice opencast mines, deposited after the last peak of the Mid-Miocene Climatic Optimum. Carbon isotopic compositions of biomarkers from Polish lignite are reported for the first time. The aim of the study is to improve the chemotaxonomic value of biomarkers by relating the results to existing paleobotanical data, and to gain information about the influencing factors on δ13C of lignite and lipids. Furthermore, biomarker and isotopic proxies are tested for their applicability in paleoclimate studies.The molecular composition of the extracted lipids is highly variable, including leaf-wax n-alkanes in the C23 to C31 range, diterpenoids, hopanoids, and angiosperm-derived triterpenoids, as well as saturated fatty acids, long-chain n-alkanols and n-alkan-2-ones. The relative abundances of mid-chain (C23, C25) n-alkanes and their 1–2‰ higher δ13C values compared to long-chain n-alkanes (C29, C31) argue for a minor contribution of macrophytes (graminoids, etc.) to peat formation, enhanced during periods of raised water level. The presence of ferruginol and dehydroferruginol testifies the contribution of Taxodiaceae. The abundances of pimarane-type diterpenoids and the presence of non-aromatic abietane-derivatives argue for the contribution of Pinaceae. Based on the presence of lupeol and lupane-type triterpenoids, an input of Betulaceae can be concluded. The contribution of further angiosperms cannot be specified based on the composition of pentacyclic triterpenoids. However, the results indicate mixed vegetation, and are in agreement with paleobotanical data highlighting abundant conifers of the Taxodiaceae/Cupressaceae and Pinaceae families, as well as angiosperms of various families (e.g., Nyssa, Quercus, Fagus), including Betulaceae (e.g., Alnus, Betula, Corylus). Based on the relationship between the carbon preference index of n-alkanes and mean annual air temperatures, obtained from a global database of peatlands, an average temperature of 24.5 °C is obtained. This value is significantly higher as estimated from paleobotanical data (15.7–19.7 °C), probably due to the influence of changes in vegetation on carbon preference index.The relative abundances of diterpenoids versus di- plus angiosperm-derived triterpenoids in detrital lignite samples revealed variable contributions of gymnosperms and angiosperms during the middle Miocene. Consistent with these results, a positive relationship exists between the di-/(di- + tri-) terpenoid biomarker ratios and δ13C of lignite samples, indicating the dominating role of varying gymnosperm/angiosperm contributions on the carbon isotopic composition of lignite. The C-isotope data of long-chain n-alkanes, diterpenoids, and angiosperm-derived triterpenoids co-vary within the profiles, arguing for an overall control of changes in δ13C of atmospheric CO2 on δ13C of plant lipids. Fluctuations in δ13C of individual compounds may also be related to changes in carbon cycling within the peat, humidity and air temperature. The influence of local variations in ambient CO2 (e.g., the canopy effect) cannot be excluded.

Effect of Diabetea tea ™ consumption on inflammatory cytokines and metabolic biomarkers in type 2 diabetes patients

Ethnopharmacological relevanceDiabetea tea™ (DT) is an anti-diabetic alternative medicine in some Asian countries. The main constituent of DT is black tea originating from Camellia sinensis that is supplemented by 12 other medicinal plants. Black tea contains a large amount of the flavonoids catechins especially epigallocatechin gallate (EGCG) which has anti-inflammatory and antioxidative capacity. This study was undertaken to evaluate the possible effects of DT intake on inflammatory cytokines, regulatory T cells (Tregs) and metabolic biomarkers in T2DM. Materials and methodsThe study included 50 patients with T2DM. The patients had received 3 cups (600ml) of DT extract or placebo (PL) extract per day during a period of 12 weeks. Intracellular cytokine expression in peripheral blood mononuclear cell (PBMC) as well as the glycemic and lipid profiles were measured at baseline and after the treatment period. The active constituents of the medicinal plants included in DT were investigated by gas chromatography-mass spectrometry (GC/MS). ResultsIngestion of DT suppressed CD4+ T cell expression of IL-1β and Il-8 (p<0.05) and up-regulated the expression of IL-10 and the Treg/IL-17 ratio (p<0.05) which was not shown in PL. A significant decrease in HbA1c and LDL was observed at the end of the study period (p<0.05) in DT. The GC/MS analyses of DT indicated the presence of lupeol, β-Amyrin and β-sitosterol. Also analyses of individual herbs showed the presence of higher levels of lupeol and β-Amyrin in Nuga Ficus bengalensis and β-sitosterol in the Attikka Ficus racemosa, indicating that the active ingredients of DT are concentrated in these two herbs. ConclusionThe present study provides evidence that DT has hypoglycemic and antihyperlipidemic properties. Interestingly, DT has anti-inflammatory effects. These properties are attributed to the flavonoids, triterpenes and phytosterol contents of the tea. We suggest that DT protects against diabetes complications in the long term.

Comparative Pharmacognostical Studies of Blue and White Flower Varieties of Clitoria ternatea l.

Objective: To establish the quality control parameters for two varieties of Clitoria ternatea among which one is medicinally important whereas second is ornamental. Method: The pharmacognostic studies were focused on macroscopy, qualitative and quantitative microscopy, physicochemical parameters, quantitative estimation of phenolics, flavonoid and also the TLC profiling with two phenolics (caffeic and ferulic acid) and two terpenoid markers (lupeol and β-sitostrol). Results: The distinguishing macroscopical feature is white and blue colour of corolla while the microscopy showed more starch grains in root; variations in pericyclic fibers, xylem vessels and pith in stem and quantitative parameters of leaf. Some variations were also observed in physicochemical parameters. However, TLC fingerprint profiles of both the varieties showed similarities in the presence of lupeol, β-sitostrol, caffeic acid and ferulic acid. However, characteristic additional bands e.g. blue fluorescent band at Rf 0.38 under UV 366 nm and greyish blue band at Rf 0.58 under visible light after derivatization were observed only in white variety. Conclusion: Present study provided the scientific data for the proper identification and establishment of standards for the two varieties of C. ternatea and blue variety may be used as substitute of white variety with less therapeutic activity.

The pentacyclic triterpene Lupeol switches M1 macrophages to M2 and ameliorates experimental inflammatory bowel disease

BackgroundInflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is a chronic inflammatory disease in the lower gastrointestinal tract. Mounting evidence suggests that the predominance of the classically activated (M1) macrophages versus the alternatively activated (M2) macrophages plays a role in the progression of IBD. Thus, agents able to shift pro-inflammatory M1 macrophages to anti-inflammatory M2 macrophages may be beneficial to IBD. The pentacyclic triterpene Lup-20(29)-en-3β-ol (Lupeol), a potent anti-inflammatory natural product, has been shown to inhibit pro-inflammatory cytokine production, suggesting it is potentially able to modulate macrophage polarization, thereby beneficial to IBD. MethodsCD4+ monocytes were differentiated to M1 or M2 macrophages, which were cocultured with epithelial cell lines, T84 and Caco-2, in the absence or presence of Lupeol (10μM). Experimental colitis was induced with dextran sodium sulfate (DSS), with or without oral administration of Lupeol (50mg/kg, q.d.). Cytokines were measured with Luminex kits. M1/M2 genes were measured with real-time polymerase chain reaction. Macrophage phenotypes were defined by measuring M1 and M2 markers with confocal microscopy. Proteins were measured with Western blotting, while cell surface markers were measured with confocal microscopy or flow cytometry. Histology was evaluated with H&E staining. ResultsTreatment of M1 macrophages with Lupeol resulted in a marked decrease in the production of pro-inflammatory cytokines, including IL-12, IL6, IL-1β and TNFα, and a marked increase in the production of IL-10, an anti-inflammatory cytokine. This was associated with a down-regulation of CD86, a typical marker of M1 macrophages, and an up-regulation of CD206, a typical M2 macrophage marker. IRF5, a transcription factor that is critically involved in M1 polarization, was down-regulated in M1 macrophages after being incubated with Lupeol, associated with a marked decrease in the phosphorylation of p38 mitogen activated protein kinase. Coculture of epithelial cells with M1 macrophages resulted in down-regulation of the tight junction protein ZO-1 and disruption of epithelial integrity, which were blocked by Lupeol treatment of the M1 macrophages. Moreover, oral administration of Lupeol to dextran sulfate sodium (DSS)-induced colitis mice resulted in mitigated intestinal inflammation and increased survival from lethal colitis, associated with decreased expression of M1-related genes and increased expression of M2-related genes. ConclusionLupeol ameliorates experimental inflammatory bowel disease through, at least in part, inhibiting M1 and promoting M2 macrophages.

Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by gas chromatography: Mass spectroscopy.

Background:Benincasa hispida (BH) and Carissa congesta (CC) are regarded as ethnopharmacological imperative plants in Asian countries.Objective:Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in the BH and CC extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography.Materials and Methods:Present research studies encompasses identification of chemical constituents in BH seeds and CC roots petroleum ether extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents.Results:The components were identified by matching mass spectra with MS libraries. There were 13 and 10 different compounds analyzed from CC and BH, respectively. The components present were Pentanoic acid, 5-hydroxy, 2,4-butylphenyl; n-Hexadecanoic acid (Palmitic acid); Sulfurous acid, 2-ethylhexylhepatdecyl ester; n-Tridecane; 6-methyltridecane; (9E, 12E)-9,12-Octadecadienyl chloride, Hexadecanoic acid, 3-(trimethylsilyl)-oxy] propyl ester; 9,12-Octadecadenoic acid, 2 hydroxy-1-(hyroxymethylethyl) ester; 9,12-Octadecadienoic acid, 2,3 dihydroxypropyl ester; n-Propyl-9,12-Octadecadienoate, Lupeol; Taraxasterol; 6a, 14a-Methanopicene, perhydro-12,4a, 61a, 9,9,12a-hepatmethyl-10-hydoxy and 9-Octadecene; 2-Isoprpenyl-5-methyl-6-hepten-1-ol; n-Hexadecanoic acid, 2-hyroxy-1-(hydroxymethyl) ethyl ether; Butyl-9,12-Octadecadieonate; Friedoolean-8-en-3-one; friedours-7-en-3-one; 13,27-Cyclosuran-3-one; Stigmaste-7,25-dien-3-ol (3β, 5α); Stigmasta-7,16-dien-3-ol; chrondrillasterol in BH seeds and CC roots extracts respectively.Conclusion:Eluted components from the extracts could provide further researchers to work with various pharmacological activities related models and studies.

Phytochemical investigation of Vernonia cinerea(Family: Asteraceae)

Phytochemical screening of Vernonia cinerea (Family: Asteraceae) showed the presence of steroids, glycosides, triterpinoids & esters in the methanolic extract of stem bark and leaves of the plant. The presence of these compounds clearly indicates different medicinal properties of V. cinerea. NMR data also confirmed the presence of Lupeol, 12-oleanen-3-ol-3s-acetate, Stigmasterol, s-sitosterol in n-hexane portion.

TLC-DENSITOMETRIC QUANTIFICATION OF NEGUNDOSIDE, URSOLIC ACID, EUGENOL, LUPEOL, AND β-SITOSTEROL USING HPTLC FROM VITEX NEGUNDO LEAVES

Vitex negundo Linn (Verberaceae) is an important drug in Indian, Chinese, and Malaysian Systems of medicine. The leaves are reported to show anti-inflammatory, anti-ulcer, anti-implantation, and analgesic activities. They contain negundoside, nishindaside, agnuside, luteolin, vitexicarpin, ursolic acid, and β-sitosterol. Lupeol, an important marker compound has not been reported from V. negundo until recently. Our preliminary TLC experiments indicated the presence of lupeol in the leaves of V. negundo. Subsequently, lupeol was isolated by preparative TLC. The identity of lupeol was confirmed by comparing the mass spectra [m/z value 427.8 (M+1) and 425.8 (M−1)]; melting point (215°C, using DSC) and co-TLC with the standard compound. A High Performance Thin Layer Chromatography (HPTLC) method for the quantification of five marker compounds, namely, negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol from the leaves of V. negundo was developed and validated as per ICH guidelines. The amounts of negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol were found to be 1.048, 0.013, 0.115, 0.116, and 0.043% w/w, respectively. The developed methods were found to be accurate, precise, and reproducible. They can be used for routine quality control of herbal material and formulations containing these compounds.

Suppression of cFLIP by Lupeol, a Dietary Triterpene, Is Sufficient to Overcome Resistance to TRAIL-Mediated Apoptosis in Chemoresistant Human Pancreatic Cancer Cells

Overexpression of cellular FLICE-like inhibitory protein (cFLIP) is reported to confer chemoresistance in pancreatic cancer (PaC) cells. This study was designed to investigate the effect of lupeol, a dietary triterpene, on (a) apoptosis of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) therapy-resistant PaC cells overexpressing cFLIP and (b) growth of human pancreatic tumor xenografts in vivo. The effect of lupeol treatment on proliferation and TRAIL/caspase-8/cFLIP machinery in PaC cells was investigated. Next, cFLIP-overexpressing and cFLIP-suppressed cells were tested for sensitivity to recombinant TRAIL therapy in the presence of lupeol. Further, athymic nude mice implanted with AsPC-1 cells were treated with lupeol (40 mg/kg) thrice a week and surrogate biomarkers were evaluated in tumors. Lupeol alone treatment of cells caused (a) decrease in proliferation, (b) induction of caspase-8 and poly(ADP-ribose) polymerase cleavage, and (c) down-regulation of transcriptional activation and expression of cFLIP. Lupeol was observed to increase the TRAIL protein level in cells. Lupeol significantly decreased the viability of AsPC-1 cells both in cFLIP-suppressed cells and in cFLIP-overexpressing cells. Lupeol significantly sensitized chemoresistant PaC cells to undergo apoptosis by recombinant TRAIL. Finally, lupeol significantly reduced the growth of human PaC tumors propagated in athymic nude mice and caused modulation of cFLIP and TRAIL protein levels in tumors. Our findings showed the anticancer efficacy of lupeol with mechanistic rationale against highly chemoresistant human PaC cells. We suggest that lupeol, alone or as an adjuvant to current therapies, could be useful for the management of human PaC.

Composés stéroliques des spores d'Anthurus muellerianus (Basidiomycète)

We have shown the presence of lupeol, ψ-taraxasterol, germanicol, and β-amyrin in the sterol fraction isolated from the spores of Anthurus muellerianus. Besides these pentacyclic triterpenes, known up to now to be present in higher plants, we have also characterized ergosterol, methyl-24 cholesta-7, 22 diene ol-3β, and methyl-24 cholestene 7 ol-3β.[Traduit par le journal]

Preliminary Phytochemical Investigation of Euphorbia millii

Euphorbia millii (Des Moulins) was investigated. β-Sitosterol, cycloartenol, and β-amyrin acetate were isolated from the petroleum ether extract. The presence of lupeol and euphol as minor constituents was demonstrated by GC, and the existence of flavonoids in the methanol extract was shown.