α-Tetralone, β-tetralone, and 1-benzosuberone were asymmetrically reduced to their corresponding alcohols by whole cells of Candida viswanathii. The cells had the maximum ketoreductase activity when grown aerobically in shake flasks (200 rpm) for 36 h in a medium consisting of fructose (30 g/L), malt extract (20 g/L), CaCl2 (1 mM), and β-tetralone inducer (1 mM) at 25°C and pH 7. The optimal bioreduction conditions with the washed cells were a pH of 6.5 (phosphate buffer, 50 mM), a temperature of 30°C, and in the presence of isopropanol (4%, v/v) as a cosubstrate. A 100% reduction with an enantiomeric excess of > 99% was achieved for β-tetralone (90 mM) and 6-bromo-β-tetralone (90 mM) using 160 g/L fresh cell concentration in 30 h. Use of a higher fresh cell concentration (250 g/L) afforded > 80% conversion of 15 mM α-tetralone in 30 h and 100% conversion of 1-benzosuberone (15 mM) in 30 h.