Presence Of Helicobacter Pylori Infection Research Articles

Detection of Helicobacter pylori infection in children using rapid urease and histologic methods of diagnosis.

The study aimed to detect the presence of Helicobacter pylori infection in children using two investigative methods: the rapid urease test and histological methods. It also examined the relationship between socioeconomic status and Helicobacter pylori infection. This was a cross-sectional study conducted in the paediatric theatre at Korle Bu Teaching Hospital in Accra, Ghana. Children who were scheduled for upper gastrointestinal endoscopy were recruited into the study. The presence of Helicobacter pylori in gastric biopsies was measured using a rapid urease test and histology. Seventy-three children aged 2 years to 16 years were seen during the period. Both tests were positive at the same time in 36 (49.3%) out of the 73 children (p<0.0001). The positivity rates for the rapid urease test and histology were 57.5% and 53.4 %, respectively. Significant predictors of the histology presence of H. pylori were a large household size of at least 6 members (AOR: 4.03; p<0.013) and the presence of pets at home (AOR: 3.23; p<0.044). Substantial agreement was found between the rapid urease test and histology examination of gastric biopsies for the presence of H. pylori. Children from large households and those with pets at home appear to have increased odds of having H. pylori infection of the gastric mucosa. None declared.

Non-alcoholic fatty liver disease and Helicobacter pylori infection: is there a relationship? Review

The article outlines the relevance of finding a connection between non‑alcoholic fatty liver disease (NAFLD) and Helicobacter pylori infection. The presented data of the majority of studies indicate the presence of Helicobacter pylori infection as a risk factor for the development of NAFLD. In addition, a number of researchers point to the possibility of the influence of Helicobacter pylori on important pathogenetic chains of NAFLD: insulin resistance, the state of the intestinal microbiota, the level of such hormones as ghrelin, leptin, adiponectin, and also that the effect of Helicobacter pylori (H. pylori) toxins can cause direct damage to the liver and that Helicobacter pylori invasion of the small intestine mucosa can increase the permeability of the latter, disrupting the functioning of the gut—liver axis. The article emphasizes the relationship between H. pylori infection and an unfavorable metabolic profile: dyslipidemia, body mass index, blood pressure, fasting blood glucose, and insulin resistance. In addition, research data on the novelty of higher levels of aspartate and alanine aminotransferase in Helicobacter‑positive patients compared to Helicobacter‑negative patients, in the absence of differences in liver fibrosis, are given. Separately, the research data are given, which indicate that the successful eradication of Helicobacter infection does not have a significant effect on liver steatosis, but probably reduces the severity of insulin resistance. Also, the authors draw attention to the fact that in a number of studies, the connection of H. pylori with NAFLD was not found, and no significant differences were found in the levels of high and low‑density lipoprotein cholesterol in people with Helicobacter infection compared to Helicobacter‑negative ones. The authors conclude that today there is no clear understanding of the role of Helicobacter pylori infection in the NAFLD pathogenesis, which requires further research because, after all, most researchers testify in favor of the fact that H. pylori infection can be one of the risk factors, contributing to the emergence and progression of NAFLD, and eradication of H. pylori may be important in the treatment of this disease.

Endoscopic diagnosis and prevalence of early gastric cancer in India: A prospective study.

Although countries like Japan and South Korea have implemented nationwide endoscopic screening programs, there is limited evidence on the effectiveness of endoscopy for diagnosing early gastric cancer (EGC) in developing countries such as India. In the present study, we aimed to determine the feasibility of endoscopic detection of EGC from India. The data was prospectively collected for all patients ≥40 years who underwent a diagnostic upper endoscopy from April to September 2021. A single endoscopist who performed the endoscopic procedures completed 1-month training in advanced endoscopy in Japan. Following the training, the endoscopist continued to engage in internet-based discussions regarding his cases encountered. Prior to this training, the endoscopist had not detected any EGC cases during his 12-year gastroenterology practice. A total of 1033 patients were included in the study, with males accounting for 65.4% and a mean age 52 years. The average procedural time was 7.13±4.8min. A total of 25 patients (2.4%) were found to have GC, including 6 patients (0.6%) with EGC. Two patients had synchronous EGC lesions. All EGC patients were males, with an average age of 66 years. All EGCs were detected in the distal stomach in the presence of Helicobacter pylori infection and severe atrophic gastritis. Our findings showed that the endoscopic detection of EGC is feasible in India. Optimal training on endoscopic diagnosis of EGC can improve the detection of such lesion. Further studies are warranted to assess the optimization and implementation of an endoscopic screening program for EGC in India.

Association between dietary zinc intake and Helicobacter pylori seropositivity in US adults: National Health and Nutrition Examination Survey.

Helicobacter pylori infection is a well-established etiological factor for gastric inflammation and a significant risk factor for the development of gastric cancer. However, the precise relationship between dietary zinc intake and seropositivity for Helicobacter pylori remains uncertain. This cross-sectional observational study utilized data from the United States National Health and Nutrition Examination Survey conducted between 1999 and 2000. The study cohort comprised 2,884 adults aged 20 years or older who provided comprehensive 24-h dietary recall data. The presence of Helicobacter pylori infection was confirmed using serum analysis and lgG protein enzyme-linked immunosorbent assay (ELISA). Multivariable logistic regression models and generalized additive model (GAM) were employed to explore the potential association between dietary zinc intake and Helicobacter pylori seropositivity. Additionally, subgroup analysis was performed to evaluate the robustness of the primary findings. Of the 1,281 participants, 47.8% were male and the average age was 49.5 years. In the fully adjusted model, a statistically significant inverse association between dietary zinc intake and Helicobacter pylori seropositivity was observed [quartile variable, Q4 vs. Q1, odds ratio (OR): 0.72, 95% confidence interval (CI): 0.57-0.91, p = 0.007]. Furthermore, the relationship between dietary zinc intake and Helicobacter pylori seropositivity exhibited an L-shaped pattern, indicating a saturation effect. The results of sensitivity analysis remained consistent and reliable. Therefore, this study suggests that higher dietary zinc intake may be associated with a lower prevalence of Helicobacter pylori seropositivity. Notably, this association follows an L-shaped pattern, with a threshold point estimated at 24.925 mg/day.

ПРОФИЛАКТИКА АНАСТОМОЗИТА ПОСЛЕ РЕЗЕКЦИИ ЖЕЛУДКА

The clinical, immunological and endoscopic features of anastomositis were studied in patients &#x0D; after the stomach resection according to Billroth II in various modifications because of peptic ulcer disease.&#x0D; The study revealed the relationship in development of anastomositis with the level of populations T-lympho cytes, immunoregulatory index, circulating immune complexes, R-proteins in the early postoperative period &#x0D; as well as before the operation. Disturbances in the immune system may lead to decrease in stability of mucosa &#x0D; and to the increase of aggressive factors. The severity of disturbances in the functioning of the immune system &#x0D; is interrelated with the presence of Helicobacter pylori infection. The higher the antibody titers to the antigen &#x0D; CagA Helicobacter pylori, the more severe there may be the manifestations of immunopathological reactions &#x0D; and the more severe the clinical signs of anastomositis. Immunological indicators and their dynamics may be &#x0D; used both for the early diagnosis of anastomositis, and for the prognosis and prophylaxis of this postoperative &#x0D; complication.

Prospects of using probiotics containing &lt;i&gt;Lactobacillus acidophilus&lt;/i&gt; in the prevention and treatment of gastrointestinal diseases

Aim theoretical analysis and systematization of the knowledge on using probiotics containingLactobacillus acidophilusin the prevention and treatment of diseases of the gastrointestinal tract.&#x0D; The bibliographic databases of the scientific libraries eLIBRARY.RU, Cochrane Library and CyberLeninka, the electronic resources of the Global Health platform, the sites of scientific publications ScienceDirect and Elsevier, MEDLINE and PubMed-NCBI were searched for the relevant information. Next, the full-text sources were analysed. We used structural-logical and analytical methods in this work.&#x0D; The probiotics containingLactobacillus acidophiluswere reported to enhance eradication therapy in the presence ofHelicobacter pyloriinfection, reduce pain and normalize bowel function in irritable bowel syndrome, normalize liver function, reduce liver enzymes, prevent the development of hepatic encephalopathy in non-alcoholic fatty liver disease, support clinical remission in ulcerative colitis. Also, they demonstrated the effectiveness for the prevention and treatment of antibiotic-associated diarrhea, including clostridial infection, and the improvement of intestinal microbiota. In many gastrointestinal diseases, the effect of probiotics had a high level of evidence. The beneficial properties of probiotics can be used in the development of functional food products for therapeutic and prophylactic purposes.

Endoscopic Findings and Histopathological Patterns of Gastric Mucosal Biopsies in Functional Dyspepsia: A Clinicopathological Study

Background: Endoscopic examination of the gastrointestinal tract through macroscopic and histopathological evaluation provides a tool to differentiate the major causes of functional dyspepsia. The distinction is not always clear. This study aimed to assess the frequency and type of the macroscopic and histopathological changes in gastrointestinal tract endoscopy in patients with symptoms of functional dyspepsia. Methods: A cross-sectional study was performed on 97 patients aged 10–85 years who underwent gastroscopy due to functional dyspepsia symptoms. The patients had no history of weight loss, major comorbidities like diabetes or cirrhosis, non-steroidal anti-inflammatory drug (NSAID) consumption, peptic ulcer, or any other confounding causes. Biopsy specimens were taken from the stomach and duodenum for histopathological examination. The presence of Helicobacter pylori infection was established based on histopathological examination and a positive rapid urease test. Results: Gastric biopsies of 97 patients with functional dyspepsia were studied. In histological examination of gastric mucosal biopsies, chronic inflammation was present in 94 (96.9%), activity was seen in 47 (48.5%), glandular atrophy was seen in 3 (3.1%), and intestinal metaplasia was seen in 9 (9.2%) patients. H. pylori was identified on gastric mucosal biopsies in 46 (47.4%) patients based on sections stained with H&amp;E and Giemsa. Conclusion: According to the obtained results, it is concluded that patients with functional dyspepsia have a higher frequency of gastric mucosal inflammation and H. pylori infection.

Assessment of the Possible Correlation between the Presence of Helicobacter Pylori Infection and Hairy Tongue Lesion in a Group of Patients in Syria: A Cross-Sectional and Pilot Study.

This study aimed to evaluate the correlation between the presence of hairy tongue and H. pylori infection in patients referring to their blood test based on the serum levels of anti-H pylori IgG antibodies. This cross-sectional study was conducted in the Department of Oral Medicine, University of Damascus Dental School, between February 2021 and January 2022. The sample size of 40 patients (23 males, 17 females), whose ages ranged from 20-79 years with a mean age of 41.5 ± 12 years, was calculated using the G*power 3.1.3, with a statistical power of 80% and a significance level of 0.05. The hairy tongue index was assessed by a visual method based on observing the dorsum tongue appearance. Then, a blood test was performed to detect the presence of H. pylori by Immulite 2000 XPi. Statistical analysis was performed using SPSS software 22.0, Chi-square. The prevalence of hairy tongue was higher among males (75%) as compared to females (25%) and was found to be statistically significant (p = 0.026). The hairy tongue lesions were found to be least in the 20-39 age group and most prevalent in the 40-59 age group, without statistically significant correlation. H. pylori infection was detected positive in 70% and negative in 30% of hairy tongue patients, compared to the control group, where the rates were 15% and 85%, respectively, with a statistically significant correlation between infection with H. pylori and hairy tongue (p = 0.001). Our results strongly suggest that the hairy tongue might be considered an indicator of H. pylori infection.

Is there any relation between the presence of Helicobacter Pylori infection and duodenogastric reflux in children?

Is there any relation between the presence of Helicobacter Pylori infection and duodenogastric reflux in children?

Outcome Prospective of Late Duodenal Perforation Repairs in Elderly

Introduction: Perforated duodenal ulcer is the communal emergency seen in surgical department, and postoperative complications of this emergency surgeries are very high which requires the related factors identification with this incidence. Aim: This study was designed to evaluate duodenal ulcer in older patients by revealing the patient's health status, early and late repair, choice of surgical procedure, and differences between older and younger duodenal ulcer patients. Study Design: A Retrospective study. Place and duration: In the Surgical Unit 2 of, Sheikh Zayed Medical College/ Hospital Rahim Yar Khan for two-year duration from January 2020 to December 2021. Methods: This study included 140 patients were alienated into 2 age groups as geriatric patients’ group (A) and adult age group (B). Patient age, gender, co-morbidities, drug use, presence of Helicobacter pylori infection, medical conditions that may have been affected by perforation, such as characteristic pain (severe upper abdominal pain or vague abdominal pain), extent of symptoms at presentation, smoking, outcomes of late repair versus early repair was recorded. Results: 140 patients were registered in the analysis. Group I (n = 70) consisted of geriatric patients (age&gt; 65) and Group-II (n = 70) adults’ patients (age &lt;65). Both groups have male dominance in this study. In both groups, the most common comorbidities were diabetes (25.7% and 8.6%, respectively) and arterial hypertension (42.8% and 41.4%, respectively), hypertension (p = 0.002) and coronary artery disease (p = 0.035) was much high in group I, while morbidity and mortality were higher in the group of elderly patients. Conclusion: Duodenal perforation is a serious disease that requires urgent surgical intervention, especially in the geriatric age. Keywords: Duodenal ulcer perforation, acute abdomen, geriatric surgery.

A Population-Based Cross-sectional Study on the Prevalence and Associated Risk Factors of Helicobacter pylori Infection in Rafsanjan, a Low Gastric Cancer Area in Southeast Iran.

Background: Despite all improvements in sanitation and exposure to antibiotics over time, Helicobacter pylori (HP) prevalence remains high, affecting the lives of about half of the world population, which can gradually lead to serious upper gastrointestinal disorders. Understanding HP's epidemiologic patterns help us to better manage the burden of this infection and to plan more effectively regarding potential screening or eradication strategies. We, therefore, aimed to report the crude and age- and sex-standardized prevalence rate of HP infection, its trend, and possible associated factors among asymptomatic healthy individuals in Rafsanjan city, a low-incidence area of gastric cancer (GC) in the southeast of Iran, from July 2018 to December 2021. Methods: This population-based cross-sectional descriptive study included 2,046 male and female subjects between 3 to 72 years who were in good health condition. Study participants were randomly selected from the Health Houses and each underwent a questionnaire-based interview and provided blood and feces samples. The presence of HP infection was detected by serum IgG antibodies and stool antigen test. Results: The overall and age- and sex-standardized prevalence rates of HP infection were 50.9% and 43%, respectively. The prevalence rate has notably decreased in all age groups compared with 2007. Also, it was significantly higher among men (P=0.033) and increased with advancing age (P<0.001). A higher population density living in a specific room area (P=0.012) increased the likelihood of HP infection. Conclusion: To conclude, the prevalence of HP infection is decreasing over time in Rafsanjan city, which may be due to improvements in living standards in this area. A healthy lifestyle and adherence to hygienic principles, especially during childhood, may be required for a reduction in the prevalence of HP infection.

Impact of serum sodium levels on Helicobacter pylori infection

Objective: Excessive salt consumption could play a role for developing gastric cancer as well as Helicobacter pylori (HP) infection. However, there is no report about the connection between serum sodium levels and HP infection. This study aimed to investigate the relationship between serum sodium disorders and HP infection. Material and Methods: In this single-center, retrospective, descriptive study, we evaluated the presence of HP infection by enzyme-linked immunosorbent assay (ELISA) among patient with serum sodium disturbances. Patients were divided into two groups as to their serum sodium levels (hypernatremia: Sodium level above 145 mmol/l, and hyponatremia: Sodium level below 135 mmol/l). Results: In total, 54 patients, half of them were hypernatremic (27), included in the study. At total, 15 (55.6%) patients tested positive for HP immunoglobulin G (Ig G) by ELISA method in hyponatremic patients, 17 (63%) patients tested positive for HP Ig G in hypernatremic patients. There was no difference between groups in terms of HP Ig G seropositivity (p=0.58). Other hand, 9 (33%) patients tested positive for HP Ig A among hyponatremic patients, 19 (70%) patients tested positive for HP Ig A in hypernatremic patients (p=0.029). Conclusion: According to our results, Hypernatremic patients have high risk for HP infection. Other hand, the presence of HP infection could be a driven-factor in the development of hypernatremia among elderly patients. Larger-scale studies are needed to reveal the relationship between hypernatremia and gastroenteritis.

Role of acid-peptic factor in the pathogenesis of the development of acid-related gastric diseasesRole of acid-peptic factor in the pathogenesis of the development of acid-related gastric diseases

Hyperacidity of the stomach plays a major role in the development of acid-related diseases of the upper gastrointestinal tract. Observance of a certain balance between reflex (nerve) and humoral factors regulating the process of acid formation contributes to the preservation of a compensatory sanogenic reaction from factors of aggression and protection of the gastric mucosa. It is shown that in the initial stage of the development of pathology, the reflex link of the regulation process of acid formation prevails. Later, with the further development of pathology, the prevalence of the humoral link already takes place. With the excessive prevalence of humoral regulation of the acid-forming function, damage to the mucous membrane occurs in the form of erosions (prevalence of 50%). In pathogenetic therapy, it is necessary to influence the reflex (nerve) link of the regulation process of acid formation at the initial stage. At the second stage, it is necessary to conduct a more selective therapy to block the humoral. In the presence of Helicobacter pylori infection in the body, it is necessary to carry out an adequate eradication therapy.

&lt;i&gt;Helicobacter pylori&lt;/i&gt; infection reduces the risk of gastroesophageal reflux disease

The aim is to study the effect of Helicobacter pylori infection on risk of developing gastroesophageal reflux disease. Materials and methods - cross-sectional observational study of 1007 patients with dyspepsia syndrome who underwent videoesophagogastroduodenoscopy with biopsy and histological examination of biopsy specimens of the gastric mucosa by OLGA-system. The age, gender, overweight, cigarette smoking, presence of Helicobacter pylori infection and gastritis stage were assessed. Results - the study showed a significant decrease in the incidence of gastroesophageal reflux disease in patients with positive H. Pylori status by 4% (RR 0,68; 95% CI, 0.49-0.94, p=0,041). The risk of developing gastroesophageal reflux disease significantly higher in overweight (RR 2,62; 95% CI 2,0-3,56; р&lt;0,001) men (RR 1,76; 95% CI 1,33-2,32; р=0,0046) who smoked cigarettes (RR 3,23; 95% CI 2,45-4,24; р&lt;0,001) and was not associated with the patient’s age and the stage of gastritis (р&gt;0,05). Conclusion - a significant reduction in the frequency and risk of developing gastroesophageal reflux disease in patients with Helicobacter pylori infection is demonstrated.

The effect of presence of Helicobacter pylori on the severity and clinical course of Rosacea

Introduction: Rosacea is manifested by erythema and telangiectasias limited to the regions of cheeks, nose, chin and forehead. Data from the literature indicate a possible causal relationship between Helicobacter pylori infection in the gastrointestinal tract and rosacea, which is confirmed by the improvement of symptoms and clinical picture of rosacea after administered Helicobacter pylori eradication therapy. The aim of the paper was to determine the frequency of Helicobacter pylori infection in patients with rosacea and to examine the effect of the therapy for eradication of infection of this microorganism on the clinical course of rosacea. Methods: Sixty patients with a diagnosis of rosacea who were treated in 2018 at the Clinic for Skin and Venereal Diseases of the University Clinical Centre of Republic of Srpska were analysed. Patients were examined during their visit to a dermatologist (first week) and after applied therapy (sixth week). Subjects were divided into two groups-group I consisted of subjects in which, in addition to rosacea, the presence of Helicobacter pylori was registered and for whom the topical therapy and triple therapy for eradication of Helicobacter pylori were administered, and group II, which included subjects in which, in addition to rosacea, the presence of Helicobacter pylori was not registered and for whom only topical therapy was administered. Statistical processing was performed in the IBM SPSS Statistics 21 software package. Results: Helicobacter pylori infection was registered in 45 % of patients. Statistical significance was determined between the presence of Helicobacter pylori infection and the presence of pustules in patients with rosacea (p = 0.027), with an occurrence of pustules significantly more frequent in patients belonging to group I (55.6 %) than in patients belonging to group II (24.2 %). Conclusion: Therapy for eradicating Helicobacter pylori infection improves the clinical course of rosacea, especially in the stage of papules and pustules.

AN ASSOCIATION BETWEEN HELICOBACTER PYLORI INFECTION AND DYSMETABOLIC SYNDROME IN ADULT EGYPTIAN POPULATION

Background: Helicobacter pylori (H. pylori) infection is considered to be one of the most popular infections worldwide, affecting more than 50% of the world's population, principally in the developing countries. Objective: To determine the possible association between Helicobacter pylori infection and dysmetabolic syndrome in adult Egyptian population, principally cardio-metabolic components. Patients and Methods: Adult patients aged more than 18 years and presented with dysmetabolic syndrome or being metabolically healthy obese were enrolled in the study. All participants were subjected to meticulous history assessment and clinical evaluation. Results: An overall 60 patients who fulfilled the inclusion criteria were enrolled in the present study. Of them, 30 patients had metabolic syndrome, whereas the remaining 30 patients were metabolically healthy obese. There was a statistically significant difference between both groups regarding the levels of systolic and diastolic blood pressure, lipid profile, and glycemic status. There was an equal proportion of patients had positive Helicobacter Pylori infection among patients with metabolic syndrome and metabolically healthy obese patients. Furthermore, among patients with metabolic syndrome, correlation analysis revealed that; BMI showed a statistically significant positive correlation with Helicobacter Pylori infection. Conclusion: Helicobacter Pylori infection was highly prevalent infection in patients with metabolic syndrome. Metabolic syndrome patients with Helicobacter Pylori infection have higher blood glucose levels together with disturbed lipid profile. Being positively correlated with H. Pylori infection, patients with high BMI should be laboratory evaluated to role out the presence of Helicobacter Pylori infection.

Infection by CagA-Positive Helicobacter pylori Strains and Bone Fragility: A Prospective Cohort Study.

Helicobacter pylori (HP) infection is a common and persistent disorder acting as a major cofactor for the development of upper gastrointestinal diseases and several extraintestinal disorders including osteoporosis. However, no prospective study assessed the effects of HP on bone health and fracture risk. We performed a HP screening in a population-based cohort of 1149 adults followed prospectively for up to 11 years. The presence of HP infection was assessed by serologic testing for serum antibodies to HP and the cytotoxin associated gene-A (CagA). The prevalence of HP infection did not differ among individuals with normal bone mineral density (BMD), osteoporosis, and osteopenia. However, HP infection by CagA-positive strains was significantly increased in osteoporotic (30%) and osteopenic (26%) patients respect to subjects with normal BMD (21%). Moreover, anti-CagA antibody levels were significantly and negatively associated with lumbar and femoral BMD. Consistent with these associations, patients affected by CagA-positive strains had a more than fivefold increased risk to sustain a clinical vertebral fracture (HR 5.27; 95% CI, 2.23-12.63; p < .0001) and a double risk to sustain a nonvertebral incident fracture (HR 2.09; 95% CI, 1.27-2.46; p < .005). Reduced estrogen and ghrelin levels, together with an impaired bone turnover balance after the meal were also observed in carriers of CagA-positive HP infection. HP infection by strains expressing CagA may be considered a risk factor for osteoporosis and fractures. Further studies are required to clarify in more detail the underlying pathogenetic mechanisms of this association. © 2020 American Society for Bone and Mineral Research (ASBMR).

Prevalence of subtypes of gastric intestinal metaplasia and its relationship with Helicobacter pylori infection

Background: Gastric intestinal metaplasia is seen chronic gastritis associated with Helicobacter pylori. The objective of this study was to determine the prevalence of subtypes of intestinal metaplasia and presence of Helicobacter pylori infection.&#x0D; Materials and Methods: This was a cross sectional study done at Kathmandu Medical College teaching Hospital in Pathology department from December 2018 to August 2019. The endoscopic biopsies were evaluated for intestinal metaplasia and Helicobacter pylori with the help of Hematoxylin and Eosin stains as well as Giemsa stain. Subtypes of intestinal metaplasia were classified with the help of periodic acid- Schiff/Alcian Blue stain combination and High Iron Diamine- Alcian Blue stain at pH 2.5. The relationship between Helicobacter pylori and subtypes of intestinal metaplasia were compared. Fisher’s exact test was used for statistical evaluation. A p value of ˂0.05 was considered as statistically significant.&#x0D; Results: The prevalence of intestinal metaplasia was found in 57 (12.2%) biopsies. Type I intestinal metaplasia was found in 23 (40.4%) biopsies, type II in 10 (17.5%) biopsies and type III in 24 (42.1%) biopsies. Helicobacter pylori was positive in 28(49.1%) and it was negative in 29(50.9%) biopsies. No statistical significant correlation was seen in the subtypes of intestinal metaplasia with Helicobacter pylori status (p˃0.05).&#x0D; Conclusion: Intestinal metaplasia is frequently observed in endoscopic biopsies, most common being type III subtype.

SUN-655 Helicobacter Pylori Infection Is Not Associated with Diabetes Among a Cohort of Diabetic Patients in Alexandria City, Egypt

: Helicobacter Pylori infection is one of the most common bacterial infections in Egypt. A mounting body of evidence suggests the association of H Pylori infection with diabetes. H.Pylori is implicated in increasing insulin resistance and promoting chronic inflammation, resulting in the development of diabetes. This study aimed to estimate the prevalence of H.Pylori infection among a cohort of patients with diabetes in Alexandria city, Egypt and the possible role of this condition in the control of the glycemic profile. We also investigated the correlation between H.pylori infection and the presence of diabetes-related complications (diabetic nephropathy and retinopathy). The study was conducted on 300 subjects classified into three groups; Group (I): 100 patients with type 2 diabetes, Group (II): 100 subjects with type 1 diabetes, Group (III): 100 non-diabetic control subjects. Participants were subjected to detailed history taking and thorough clinical examination. Routine laboratory investigations were done, including HbA1c and fasting plasma glucose. Stool antigen test, on- site Helicobacter Pylori Ag Rapid Test-cassette was done. The mean duration of diabetes in type 2 diabetes was 8.18±5.87 years, while the mean duration in type 1 was 4.88± 3.02 years, which was statistically significante (p< 0.05). The results of the presented study showed that there was no significant difference in the prevalence of Helicobacter Pylori infection between type 1 and type 2 patients with diabetes (31% Vs 38%, p=0.298), moreover; after adjustment for age, there was no significant difference in the prevalence of Helicobacter Pylori infection among either group with diabetes (Group 1 and group 2) compared to the control group, (p= 0.756 and 0.066) respectively. There was no statistically significant association between the presence of Helicobacter Pylori infection in both type 1 and type 2 diabetes, and an elevated HbA1c level above 6.5%.(p= 0.772 and p=0.524) respectively. The prevalence of diabetic nephropathy in patients with type 2 and type 1 diabetes was 5% and 3% respectively, which was non-statistically significant (p=0.721). While the prevalence of diabetic retinopathy was 11% among patients with type 2 compared to 1% among patients with type 1 diabetes, which was statistically significant (p=0.003). There was no statistically significant correlation between Helicobacter Pylori infection and the presence of diabetic nephropathy or diabetic retinopathy. Helicobacter Pylori infection was not associated with diabetes and did not affect the HbA1c level. Helicobacter Pylori infection was not correlated to the presence of either diabetic nephropathy or diabetic retinopathy among both patients with type 2 and type 1 diabetes.Nothing to Disclose: MA, MM, AHNo Sources of Research Support

The impact of DNA demethylation on the upregulation of the NRN1 and TNFAIP3 genes associated with advanced gastric cancer.

Gastric cancer (GC) is the third leading cause of cancer-related death worldwide. Very few therapeutic options are currently available in this neoplasia. The use of 5-Aza-2'-deoxycytidine (5-AZAdC) was approved for the treatment of myelodysplastic syndromes, and this drug can treat solid tumours at low doses. Epigenetic manipulation of GC cell lines is a useful tool to better understand gene expression regulatory mechanisms for clinical applications. Therefore, we compared the gene expression profile of 5-AZAdC-treated and untreated GC cell lines by a microarray assay. Among the genes identified in this analysis, we selected NRN1 and TNFAIP3 to be evaluated for gene expression by RT-qPCR and DNA methylation by bisulfite DNA next-generation sequencing in 43 and 52 pairs of GC and adjacent non-neoplastic tissue samples, respectively. We identified 83 candidate genes modulated by DNA methylation in GC cell lines. Increased expression of NRN1 and TNFAIP3 was associated with advanced tumours (P< 0.05). We showed that increased NRN1 and TNFAIP3 expression seems to be regulated by DNA demethylation in GC samples: inverse correlations between the mRNA and DNA methylation levels in the promoter of NRN1 (P< 0.05) and the intron of TNFAIP3 (P< 0.05) were detected. Reduced NRN1 promoter methylation was associated with III/IV TNM stage tumours (P= 0.03) and the presence of Helicobacter pylori infection (P= 0.02). The identification of demethylated activated genes in GC may be useful in clinical practice, stratifying patients who are less likely to benefit from 5-AZAdC-based therapies. KEY MESSAGES: Higher expression of NRN1 and TNFAIP3 is associated with advanced gastric cancer (GC). NRN1 promoter hypomethylation contributes to gene upregulation in advanced GC. TNFAIP3 intronic-specific CpG site demethylation contributes to gene upregulation in GC. These findings may be useful to stratify GC patients who are less likely to benefit from DNA demethylating-based therapies.