The presence of arsenic in water poses a serious threat to both human health and natural systems, and is a global chronic issue. A comprehensive study on the water sample of Taptapani Hot Spring Odisha, India was carried out to isolate Arsenic (III) bioremediating microbial strains. 42 different bacterial isolates were screened and among them, phylogenetic analysis revealed the strains showing improved metal tolerance test adaptivity at various concentrations of Arsenic (III) to be closely related to Exiguobacterium sp. (SSB11), Alcaligenes faecalis DZ2 (SSB17) and Lysinibacillus sphaericus SI-3 (SSB58) and hence selected. The findings revealed that the consortium of thermophilic isolates was more effective at adsorbing Arsenic (III) than single isolates. The analysis of variance (ANOVA) revealed a significant coefficient of determination value and accurately forecast second order regression. The isotherm model had been applied for the first time to evaluate the arsenic adsorption efficacy of hot spring bacterial isolates, and the data were en suite to pseudo-first and second-order models. The kinetic data were fitted to Langmuir and Freundlich models. The most favorable elimination circumstances have been resolute and under optimal conditions, the adsorption of Arsenic (III) was found to be 50.28%.