According to the European Regulation on cosmetic products, the presence of acrylamide in these consumer products is not allowed due to its mutagenic and potentially carcinogenic effects. Despite this ban, acrylamide might be present in those cosmetic products containing acrylamide-based polymers. However, there is no analytical method for its determination in this type of matrices. Based on this, the development of analytical methods for the determination of acrylamide in cosmetic products is required to guarantee consumer safety. In this work, an analytical method for determining traces of prohibited acrylamide in cosmetic products is presented for the first time. The method is based on dispersive liquid-liquid microextraction (DLLME), followed by liquid chromatography–ultraviolet (LC-UV) detection. A previous derivatization of acrylamide with 2-naphthalenethiol was performed to obtain a more lipophilic compound, and therefore, more extractable in the DLLME step, in addition to the bathochromic displacement of the measurement wavelength. The main variables involved in both derivatization and DLLME step were studied to provide the best enrichment factors. Under the optimized conditions, 6.5 mL of aqueous sample solution was cleaned-up from lipophilic matrix components by liquid-liquid extraction (LLE) using 3.5 mL of hexane. Then, 5 mL were taken and subjected to microwave derivatization with a mixture of 250 μL of 2-naphthalenethiol (0.5 mg mL−1) and 250 μL of di-sodium tetraborate (4 mg mL−1). Afterwards, it was subjected to DLLME by injecting a mixture of 250 µL of acetone (as disperser solvent) and 80 µL of chloroform (as extraction solvent). The extracts were evaporated to dryness, reconstituted in mobile phase and injected into the LC-UV system. The method was successfully validated showing good linearity (from 10 ng mL−1 to 20 µg mL−1), an enrichment factor of 103 ± 2, instrumental limits of detection and quantification of 3.0 and 9.8 ng mL−1, which, according to sample treatment, correspond to 0.7 and 2.4 µg kg−1 in the sample, respectively, and a good repeatability (RSD < 14%). Finally, the proposed analytical method was applied to the determination of acrylamide in commercial cosmetic samples of different nature with good relative recovery values (85 – 112%), which shows that the matrix effect is negligible. This method could help the cosmetic industry to control the cosmetic products containing potential acrylamide-releasing ingredients.