This study assessed the understanding of dentists regarding the appropriate prescription of aminoglycosides and macrolides in Aden, Yemen. We carried out a cross-sectional study, enlisting a total of 150 licensed dentists from both public and commercial dental clinics. The data was gathered throughout the period of October to December 2023. Out of the 150 participants, 57% were men, and 47% had less than 5 years of experience. According to the poll, a large majority of dentists, 72%, use macrolides on a regular basis in their practice. In addition, azithromycin was the most commonly prescribed macrolide, accounting for 18% of total prescriptions. By comparison, aminoglycosides account for only 21% of all prescriptions, and neomycin is quite rare. 13% of persons with penicillin allergies were administered streptomycin, underscoring the necessity for safer alternatives. Dentists typically follow the prescribed time frame of 3-5 days to ensure the appropriate administration of macrolides. The findings indicated that individuals should get instruction on the proper utilization of aminoglycosides and macrolides, be provided with comprehensive information to enable them to make well-informed decisions on their treatment alternatives, and be obligated to adhere to guidelines grounded in empirical evidence from real-life scenarios. Effective antibiotic stewardship practices in Yemeni dentistry require collaboration among dentists, microbiologists, and public health officials. It is recommended to undertake additional research to expand the study's scope and include observational studies and interviews with dentists.