Applying manure to alfalfa (Medicago sativa L.) poses a potential risk for overloading the soil system with N. The objective of this study was to evaluate the effect of preplant nutrient treatments on alfalfa herbage and crown root N and soil nitrate-N. The effects of three preplant manure rates (3 000, 6 000, and 12 000 gal/acre) and three equivalent P and K fertilizer rates were investigated at Rosemount and Waseca, MN, on a Waukegan silt loam (fine-silty over sandy or sandy skeletal, mixed mesic Typic Hapludolls) and on a Nicollet clay loam (fine-loamy, mixed, mesic Aquic Hapludolls), respectively. Alfalfa herbage N removal was highly correlated to herbage dry matter yield. Herbage N removal in the establishment year was greater for the manure treatments than the P and K fertilizer treatments at the Rosemount sites. The average herbage N removal in the seeding year was 229 lb N/acre for Rosemount-North and in the production year was 278, 341, and 233 lb N/acre for Rosemount-South, Rosemount-North, and Waseca, respectively. Measured soil nitrate-N in plots receiving manure peaked 30 to 50 d after manure application, averaging 328 lb/acre, and decreased throughout the growing season. Peak nitrate-N amounts were proportional to the amount of manure applied. After the second alfalfa growing season, soil nitrate-N amounts were not significantly different among treatments. This study's data indicate that preplant manure applications do not result in consistently more N in the soil or in the plant after 2 yr of alfalfa. Research QuestionDairy producers in the Upper Midwest have traditionally applied manure to corn ground. When manure storage capacity or handling systems necessitate, manure is often topdressed on alfalfa. Because topdress manure is often detrimental to alfalfa, preplant applications warrant consideration. The primary objective of this research was to evaluate the effect of preplant manure and fertilizer P and K treatments on N removal by alfalfa and soil nitrate-N amounts during 2 yr of alfalfa production. Literature SummaryNumerous surveys have documented the overapplication of N on corn fields in traditional dairy livestock enterprises. This, along with the necessity of summer manure applications, has prompted some farmers to apply manure on alfalfa. While alfalfa has been shown to use residual soil N, alfalfa herbage production and N removal in association with soil N levels created by manure additions are important due to the environmental concerns of excess N in the soil system. The effects of manure-N on plant and soil N accumulation need further investigation. Study DescriptionOur study was conducted in southeast Minnesota on one site that tested “medium” and two sites that tested “very high” for P and K. Three rates of manure (3 000, 6 000, and 12 000 gal/acre) and three equivalent rates of P and K fertilizer, along with a control, were applied preplant for a 2-yr stand of alfalfa. Applied QuestionsIs N removal by alfalfa or accumulation in the soil affected by manure application rate? Nitrogen removal by alfalfa was positively correlated with forage yield. In the season following the seeding year, manure rate did not have an effect on herbage N removal at the Rosemount sites, which had high P and K soil tests (Fig. 1). The lack of herbage N removal differences indicates that plant N fixation may have varied among plots. At Waseca, with the medium-testing soils for P and K, both manure and fertilizer treatments increased herbage N removal in proportion to the application rate in the production year. Soil nitrate-N amounts decreased rapidly the first summer after appliction for all sites (Fig. 2). After an increase in soil nitrate-N for the control and 3 000 gal/acre treatment in the spring of the second season, nitrate-N amounts decreased and by the end of the second season the soil nitrate-N amounts for the manure treatments and the control were similar. In the establishment year, the soil nitrate-N increases combined with herbage N removal could not account for all of the applied N in manure—total or inorganic. This indicates that appreciable amounts of N can be immobilized, denitrified, or volatilized after application. Fig. 1Herbage N removal in the year following seeding as affected by preplant manure or fertilizer treatments. Fig. 2Soil nitrate-N amounts (0–2 ft) averaged across all three sites as influenced by rate of manure application and time after application.