Tobacco (N. tabacum L.) is an annual plant belonging to the family Solanaceae, genus Nicotiana. The first information of the use of tobacco as a medicinal plant data back to 1492. After the isolation of the alkaloid nicotine from tobacco leaves in 1828 and the establishment of its addictive effect, the medical world ceased to use tobacco as a treatment. Several centuries later, scientific research has resumed and it is again directed towards a detailed study of the biologically active substances in tobacco and its alternative use in medicine, bio-engineering and biotechnology. Organic tobacco is a new industrial plant product that is grown on certified bio fields, without using conventional fertilizers and plant protection preparations. The nicotine content is significantly lower than that of tobacco grown under conventional conditions. It is of interest to study the quantitative and qualitative composition of some secondary metabolites in organic tobacco extracts, like total phenolic content, obtained by maceration with different polar solvents, and the comparison with selected medicine plants extracts - thyme, hawthorn, horsetail, nettle, and dandelion. Our research found that bio tobacco has a high content of phenolic acids close to the content of phenolic acids in thyme and is higher than that of the medicinal plants horsetail, dandelion, hawthorn and nettle. Bio tobacco extracts have high antioxidant activity determined by ABTS, DPPH, HPSA, FRAP, CUPRAC methods, similar to that of thyme extracts activty. Bio tobacco extracts have significantly higher antioxidant activity than hawthorn, nettle, horsetail and dandelion extracts.