Preparation Of Kits Research Articles

The eukaryotic expression of SAP-2 shows higher sensitivity than the prokaryotic expressed SAP-2 protein in the detection of antibodies against bovine fasciolosis.

Bovine fasciolosis, caused by Fasciola gigantica and Fasciola hepatica, represents a major economic burden to the livestock industry. Developing a reliable diagnostic antigen is crucial for advancing diagnostic kits for bovine fasciolosis, which could effectively mitigate these economic losses. FgSAP-2 has demonstrated​​​ considerable potential as a diagnostic antigen when expressed in prokaryotic systems. Computational analyses suggest that FgSAP-2 undergoes glycosylation, promoting the investigation of whether eukaryotic expression systems--capable of performing post-translational modifications--might enhance its antigenic properties and improve its suitability for diagnostic kit development. To explore this possibility, FgSAP-2 was expressed in Pichia pastoris (reFgSAP-2), purified and used to establish an indirect ELISA. The sensitivity, specificity, and stability of the ELISA were subsequently evaluated. Field serum samples from Guangxi were tested using the reFgSAP-2-based ELISA and compared to results from ELISAs employing prokaryotically expressed FgSAP-2 (rpFgSAP-2) and Excretory-Secretory Products (FgESP). The reFgSAP-2 ELISA exhibited positive detection at a serum dilution of 1:1600, with a coefficient of variation (CV) below 10 % in both intra-batch and inter-batch repeatability tests. Furthermore, no cross-reactivity was observed with sera positive for Schistosoma japonicum and Toxoplasma gondii. The positive detection rates of the reFgSAP-2-ELISA were comparable to those of the FgESP-ELISA, both surpassing the detection performance of the rpFgSAP-2-ELISA. CONCLUSION: An indirect ELISA detection method based on eukaryotically expressed FgSAP-2 was successfully developed, demonstrating high sensitivity, specificity, and repeatability. This approach shows promise for further development in the preparation of diagnostic kits.

Construction of fluorescent biosensor based on aptamer recognition and enzyme-free nucleic acid signal amplification reaction and its application in cocaine detection

Cocaine is a white crystalline alkaloid extracted from coca leaf. It can block human nerve conduction, produce local anesthetic effect, and has strong addiction. Therefore, the qualitative and quantitative analysis of cocaine is of great significance in forensic toxicology, pharmacy and metabolomics. Cocaine aptamer probe is expected to become a powerful tool for on-site detection of cocaine due to its good stability, fast response speed, and easy preparation of kits for on-site application. In our work, we construct a fluorescent biosensor for the detection of cocaine by using the characteristics of specific binding of nucleic acid aptamers to targets and combining with enzyme-free nucleic acid signal amplification reaction. First, streptavidin-modified magnetic beads (MBs) can specifically bind to biotin-modified cocaine aptamer probe (Aptamer) to form MB-DNA. The nucleic acid aptamer complementary probe (tDNA) can hybridize with the aptamer through base complementary pairing to form a double strand, thereby obtaining the MB-DNA-tDNA complex. When cocaine is present in the system, cocaine specifically binds to aptamer, thereby replacing the probe tDNA. The probe tDNA can undergo hybridization chain reaction with the hairpin probe 1 (HP1) and hairpin probe 2 (HP2), resulting in the opening of the hairpin structure, and the fluorescence signal response of the fluorophore 6-carboxyfluorescein (FAM) modified on the HP1 is turned on, so as to achieve rapid, low-cost and efficient cocaine detection. The establishment of this method can enrich and develop the basic theory of drug analysis and detection, and provide theoretical guidance and technical support for the accurate, efficient and convenient detection of drug analysis methods on site.

АВТОМАТИЗИРОВАННАЯ СИСТЕМА ПОДГОТОВКИ ИНСТРУМЕНТАЛЬНЫХ КОМПЛЕКТОВ ДЛЯ ОБРАБАТЫВАЮЩЕГО ОБОРУДОВАНИЯ С ЧПУ

The article describes an automated system for the preparation of tool kits for CNC machining equipment, designed to improve the efficiency of solving a number of tasks related to the organization of information and material flows at an automated site of CNC machining equipment.

The current status and factors related to the preparation of home first-aid kits in China.

Home first-aid kits can play an important role when residents are injured, suddenly become seriously ill or suffer from disasters. To explore the home first-aid kit preparedness of Chinese residents and the relationship between demographic sociological characteristics, self-efficacy, Big Five personality, health literacy and home first-aid kit preparation behavior. A cross-sectional study was conducted. Information was collected through a self-designed questionnaire consisting of sociological characteristics, the New General Self-Efficacy Scale (NGSES), the Health Literacy Scale Short-Form (HLS-SF12), and the 10-item short version of Big Five Inventory (BFI-10). Rank sum test, Chi-square test, and logistic regression were used to explore the relationship between independent variables and home first-aid kit preparation behavior. A total of 9,344 respondents were included, and 2,156 (23.07%) prepared home first-aid kits. Among the respondents who had prepared their home first-aid kits, disinfection supplies (85.20%), medical masks (84.51%), commonly used drugs (82.79%) were the most frequently available. The respondents whose geographic area was Central and Western China, permanent residence in the urban area, secondary education or above, monthly income of 3,000 RMB or above, health care cost-bearing method mainly resident health insurance, high subgroup of self-efficacy, high subgroup of health care dimension in health literacy, and whose openness and conscientiousness was high subgroup were more likely to prepare home first-aid kits (P < 0.05). The preparation rate for home first-aid kits in China is less than a quarter. The public's home first-aid kit preparation behavior is related to demographic characteristics, self-efficacy, health literacy, and the openness of the Big Five personality. A multi-level linked national emergency literacy education system should be established to enhance the residents' attention to home first-aid kits and improve the residents' ability to prevent emergencies.

The presentation of clinical results of radiolabelling of 99mTc-MIBI complex using ultrasound technique for myocardial perfusion SPECT scanning

BackgroundThe aim of this study was to introduce a new clinical procedure (ultrasound irradiation technique) as an alternative method for the preparation of sestamibi kits as opposed to the boiling water bath technique. The main proposal of this study was to evaluate the clinical application of ultrasound method, preparation of 99mTc-MIBI complex and biodistribution of the heart in comparison with 99mTc-MIBI complex, which was prepared using the boiling water technique. Materials and methodsForty MIBI kits were supplied for twenty patient volunteers. 740–3700MBq (20–100mCi) freshly eluted solution of Na+99mTcO4− was added to freeze-dry MIBI Kits (The first day up to 20 kits and the second day another 20 kits with the same amount was used). Moreover, twenty kits were labeled applying the ultrasound radiation method as an alternative approach (The vials were sonicated in thermo noted bath (Elma, P=50W, Germany) at 65±0.5̊C and for 60±1seconds), and another twenty kits were provided applying boiling water bath method as a standard approach. Twenty patient volunteers (8 men and 12 women; age range 30–72, median 52.45 years) took part in this research. These volunteers had originally been referred to the hospital for myocardial perfusion imaging. The twenty volunteer patients were divided into two groups (each group contained ten people): group A (consisted of 3 men and 7 women with an age range from 36 to 67 years and a mean age of 51.7 years), and group B (involving 5 men and 5 women with an age range from 30 to 72 years and an average age of 50.3 years). The 99mTc-MIBI complex samples provided by ultrasound method were injected into group A subjects intravenously and group B received 99mTc-MIBI vials provided by boiling water bath approach (intravenous injection). In this research, the myocardial perfusion-SPECT was performed applying a rest-stress protocol which is carried-out between two isolated days for all volunteer patients. ResultsThe results obtained in this research applying radio-HPLC and ITLC, suggest that radio-labeling of MIBI vial with 99mTc can be achieved with high efficacy and appropriate acceptance applying the ultrasound method as a novel approach. The imaging research applying SPECT indicated that the 99mTc-MIBI complex samples that were prepared applying an ultrasound irradiation approach indicate very similar biodistribution in the heart, thyroid, lung, liver, gallbladder, kidneys, stomach, GI and bladder of the volunteer patients. Non-habitual aggregation of 99mTc-MIBI samples were not observed in our approach. ConclusionsThe results of this research suggest that the sonication approach can be proposed for preparation of 99mTc-MIBI radio-complex samples. The reaction time to provide radio-complex can be considerably decreased compared to the boiling water bath approach. This main development could decrease the potential risk to the patient to inhibit any delay in emergency situations i.e. acute therapy, especially for myocardial infarction patients in clinical practice.

TCF7L2 Polymorphisms in Type 2 Diabetes, Insight from HRM and ARMS Techniques

IntroductionDiabetes is a biological problem of life in a new way in human societies, especially in developing countries. Environmental and genetic factors are mutually influential in the incidence and exacerbation of this disease. One of the genes is the transcription factor TCF7L2 that has been proven in many studies in different communities to play a role in diabetes and is located on chromosome 10. Two SNPs for this gene, rs12255372 and rs7903146, have been recorded on the NCBI site and have a direct and significant correlation with type 2 diabetes. In this study, the genotypic frequency of these two SNPs was studied using ARMS and HRM techniques.MaterialsThis study was performed on 100 patients with type 2 diabetes and 100 healthy individuals as non-diabetic controls. Diabetics were selected from patients referred to Ali Asghar Diabetes Clinic in Zahedan. The control group consisted of individuals who did not meet the criteria for diabetes or had no family history of diabetes in first- or second-degree relatives. DNA extraction was performed using the phenol-chloroform method and finally, PCR was performed for a specific primer.ResultsARMS results showed that the number of people with SNP rs7903146 ​​in diabetic patients is significantly higher than those in the control. However, the difference was not significant for rs12255372. HRM results were also highly correlated with ARMS and showed very precise allelic differentiation in the studied population for both positions.ConclusionIn general, since HRM is a relatively inexpensive technique and a large number of samples can be analyzed in a few hours, the results of this study can be used in the preparation of diagnostic kits based on this method in these two and other sites related to diabetes.

Transferability of a two-strip method for the quality control of technetium-99m mercaptoacetyltriglycine ([99mTc]Tc-MAG3)

ObjectivesTechnetium-99m mercapto-acetyl-triglycine ([99mTc]Tc-MAG3) is a radiopharmaceutical diagnostic agent used in nuclear medicine intended for the exploration of nephrological and urological disorders. Patient safety and reliability of this imaging procedure especially...

Improvement of the Pre-Analytical Phase of Nasopharyngeal Samples During the COVID 19 Pandemic: Experience of the Institute Pasteur De Côte d’Ivoire in 2020

The clinical laboratory is playing an increasingly important role in the patient-centered approach to the delivery of health services. Early detection of the importation of COVID-19 is a crucial challenge for all countries at risk of importation from areas of active transmission. This article aims to share the experience of the IPCI in the optimum management of the pre-analytical phase related to diagnostic tests for COVID 19. From the start of the pandemic the state and the IPCI made several organizational decisions to respond effectively to all trends of the COVID 19 epidemic in our country. Outsourcing of pre-analysis with creation of several sections: preparation of tube kits, aliquoting of samples from patients suspected of COVID19, reception, sorting and decontamination. Exemplary use of administrative and laboratory support staff. Our findings should help establish urgent priorities for intensified preparedness and response support in specific African countries with moderate or high risk of COVID-19 importation and whose capacity to manage the health emergency is low relatively low.

EVALUATION OF THE DOMINANT PROTEIN FROM WHOLE-CELL HEATING METHOD USING MALDI-TOF/TOF MASS SPECTROMETRY

Immunoglobulin G (IgG) are the essential tools in diagnostic kits. The whole-cell is still considered to be used for animal‘s immunization to produce IgG. The goal of this study was to determine dominant protein on the Shiga toxin-producing Escherichia coli (STEC) O157:H7-induced typical antibody response also determined by MALDI-TOF/TOF mass spectrometry. The heating method was conducted at 71 °C and 83 °C for antigen preparation. The proteins were characterized using SDS-PAGE and evaluated by MALDI-TOF/TOF mass spectrometry. The present study reported that the dominant polyclonal antibody inducing protein was ompC E. coli. The evaluation of mass spectra by MALDI-TOF/TOF might help to identify the dominant polyclonal antibody inducing protein in future preparation of diagnostic kits.

Ten Myths about Medical Emergencies and Medical Kits

ABSTRACTWilderness medicine is plagued by myths and dogmatic teachings not supported by evidence. This article focuses particularly on those teachings and tools that would be most likely used in archaeological fieldwork. It lays out 10 of the most common and concerning myths taught in wilderness medicine and wilderness emergency medical services, both in terms of first aid and preparation of medical kits. The myths described are provide a structure for the main purpose of the article: to explain interventions and medical kit contents that are more evidence based and supported by modern understandings of wilderness medicine and fieldwork risk management. The list of top 10 myths includes (1) the use of medications other than epinephrine for anaphylaxis and (2) the availability and proper use of epinephrine auto-injectors, (3) the use of suction devices and tourniquets for snakebites, (4) the use of spinal immobilization for neck injuries, (5) the identification and treatment of heat illnesses, (6) the use of CPR in remote areas, (7) the appropriateness of dislocation reduction in remote areas, (8) the use and choice of tourniquets for arterial bleeding, (9) the initial definition and management of drowning patients, and (10) wound management myths.

More from less: Study on increasing throughput of COVID-19 screening and testing facility at an apex tertiary care hospital in New Delhi using discrete-event simulation software.

BackgroundOne of the challenges has been coping with an increasing need for COVID-19 testing. A COVID-19 screening and testing facility was created. There was a need for increasing throughput of the facility within the existing space and limited resources. Discrete event simulation was used to address this challenge.MethodologyA cross-sectional interventional study was done from September 2020 to October 2020. Detailed process mapping with all micro-processes was done. Patient arrival patterns and time taken at each step were measured by two independent observers at random intervals over two weeks. The existing system was simulated and a bottleneck was identified. Two possible alternatives to the problem were simulated and evaluated.ResultsScenario 1 showed a maximum throughput of 316. The average milestone times of all the processes after the step of “Preparation of sampling kits” jumped 62%; from 82 to 133 min. Staff state times also showed that staff at this step was stretched and medical lab technicians were underutilized. Scenario 2 simulated the alternative with lesser time spent on sampling kit preparation with a 22.4% increase in throughput, but could have led to impaired quality check. Scenario 3 simulated with increased manpower at the stage of bottleneck with 26.5% increase in throughput and was implemented on-ground.ConclusionDiscrete event simulation helped to identify the bottleneck, simulate possible alternative solutions without disturbing the ongoing work, and finally choose the most suitable intervention to increase throughput, without the need for additional space allocation. It therefore helped to optimally utilize resources and get “more from less.”

Improvement of reproducibility and thermal stability of surface-enhanced Raman scattering-based lateral flow assay strips using silica-encapsulated gold nanoparticles

A surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) strip using silica-encapsulated gold nanoparticles (Si-AuNPs) was developed for the diagnosis of mosquito-borne diseases—dengue fever, Zika virus, or malaria—which are endemic to tropical regions. To investigate the thermal stability of the SERS nanoprobes, Raman peak intensity variations for AuNPs and Si-AuNPs were measured and compared at 45°C. SERS mapping images of target human immunoglobulin (IgG) on the test line of the Si-AuNP-loaded LFA strip were also evaluated at the same temperature to test the reproducibility of detection. In this case, the distribution of Raman mapping points on the test line was relatively uniform compared with the AuNP-loaded LFA strips at 45°C. This indicates that the silica encapsulation on the surface of the AuNPs improves the reproducibility of the SERS nanoprobes at high temperatures. The limit of detection (LOD) value of the AuNP-loaded LFA strips was significantly increased when the temperature was increased from 25°C to 45°C. In the case of the Si-AuNP-loaded LFA strips, however, the LOD value at 45°C was almost the same as that at 25°C. This result also shows that the silica encapsulation of the AuNPs improves the thermal stability of the LFA strips at high temperatures. Thus, Si-AuNPs are reproducible and thermally stable labeling agents for use in LFA strips at high temperatures and are suitable for the preparation of rapid kits, which can be used in tropical areas.

Preparation of ELISA and Lateral Flow Kits for rapid Diagnosis of Mycoplasma gallisepticum in Poultry

Avian mycoplasmas were mainly the cause of poultry industry economic losses; reduced meat and egg production and increases the antibiotic treatment cost. Mycoplasma gallisepticum (MG) infection is designated as infectious sinusitis of turkeys and chronic respiratory disease of chickens (gasping, depression, semi closed eyes, infraorbital sinuses edema and decrease in egg production). This study aimed to prepare, evaluate and Compare in-house ELISA kits and lateral flow assay (LFA) from a local strain of MG with commercial ELISA kits and PCR consequently. A total of 54 samples (27 tracheal swabs, 10 trachea and 17 lung) and 50 serum samples collected from birds suffering from chronic respiratory disease were tested by prepared in-house ELISA, commercial ELISA kits, PCR and LFA; a high correlation coefficient between in-house ELISA using whole antigen or sonicated antigen and commercial kit was recorded. Lateral Flow assay (LFA) performance indicate a low sensitivity (77.5%) but maintain a high specificity (92%) compared to PCR. The in-house ELISA kits and LFA prepared could be used as a fast diagnostic technique for detection of MG in Egypt. According to the available knowledge the prepared LFA for diagnosis of MG infection in chickens was developed for the first time in Egypt.

Dual Robot Kit Preparation in Batch Preparation of Component Kits for Mixed Model Assembly

Kitting is a materials supply principle that plays a vital role for performance in mixed model assembly systems. The kit preparation process, whereby component kits are created, is central when kitting is applied. Kit preparation is a form of materials handling and is associated with several ergonomic and quality related issues. Robotics holds a great potential for decreasing the need for human labour, but literature on the topic is scarce. The purpose of this paper is to identify the time efficiency potential of a dual robot application for kit preparation. To address the purpose, a mathematical model is developed that allows dual robot kit preparation to be analysed and compared with manual kit preparation. Furthermore, the model supports identification of a suitable batch size given a lead time requirement from the assembly system. A numerical example shows dual robot kit preparation to be slightly more efficient than its manual ditto for preparation of 2, 3 and 4 kit batch sizes. The paper makes a theoretical contribution in terms of the time efficiency model of dual robot kit preparation. This model is also useful for practitioners when evaluating the potential of dual robot arm kit preparation in their own processes.

Modelling time efficiency of cobot-supported kit preparation

Kitting – meaning to supply assembly with components in presorted kits – is widely seen as beneficial for assembly quality and efficiency when there is a multitude of component variants. However, the process by which kits are prepared – the kit preparation – is labour-intensive, and kit errors are problematic at assembly processes. The use of robotics to support kit preparation has received some attention by researchers, but literature is lacking with respect to how collaborative robots – cobots – can support kit preparation activities. The purpose of this paper is to identify the potential of a cobot to support time-efficient batch preparation of kits. To address the purpose, the paper presents a mathematical model for estimation of the cycle time associated with cobot-supported kit preparation. The model is applied in a numerical example with experimental data from laboratory experiments, and cobot-supported kit preparation is compared with manual kit preparation. The findings suggest that cobot-supported kit preparation is beneficial with diverse kits and smaller components quantities per SKU (Stock Keeping Unit) and provides less variability of the outcome, when compared to manual kit preparation. The paper reveals several insights about cobot-supported kit preparation that can be valuable for both academics and practitioners. The model developed can be used by practitioners to assess the potential of cobots to support kit-batch preparation in association with assembly, spare parts, repair and maintenance, or business to business industry.

Pediatric Pharmacists' Participation in Cardiopulmonary Resuscitation Events.

The Pediatric Pharmacy Advocacy Group (PPAG) understands the dilemma and varying factors that many institutions face concerning the routine participation of pharmacists in emergency resuscitation events. Acknowledging these obstacles, the PPAG encourages all institutions to strongly consider creating, adopting, and upholding policies to address pharmacists' participation in cardiopulmonary resuscitation (CPR) as evidenced by the impact pharmacist participation has shown on the reduction of hospital medication error and mortality rates in children. The PPAG advocates that pharmacists be actively involved in the institution's CPR, medical emergency team committees, and preparation of emergency drug kits and resuscitation trays. The PPAG advocates that all institutions requiring a pharmacist's participation in CPR events consider adoption of preparatory training programs. Although the PPAG does not advocate any one specific program, consideration should be taken to ensure that pharmacists are educated on the pharmacotherapy of drugs used in the CPR process, including but not limited to basic life support, Advanced Cardiac Life Support, and Pediatric Advanced Life Support algorithms; medication preparation and administration guidelines; medication compatibility; recommended dosing for emergency medications; and familiarity with the institutional emergency cart.

Pembuatan Training Kit Pneumatik untuk SMKN I Ngawen Kabupaten Gunungkidul

Community Service Activities (PKM) of Department of Mechanical Engineering (DTM), Vocational College, Universitas Gadjah Mada 2015 is entitled the development of pneumatic training kit for SMK N I Ngawen, Gunungkidul Regency. The school located in Jono Hamlet, Tancep Village, Ngawen District, Gunungkidul Regency, has a land area of 15,300 m2. There are four competency expertise held in this school, namely: Light Vehicle Technique, Heavy Equipment, Computer and Network Engineering, and Batik Clothing. SMK N I Ngawen and Vocational College (in this case Mechanical Engineering Department as a PKM team directly involved) have established cooperation in the field of heavy equipment education. SMK N I Ngawen actively has planning and effort of developing PLC control technique as a tool of practice to support the competence of machine expertise. After conducting surveys and coordination with the Principal and its staff, the PLC's control engineering development activities are planned to include scheduling, coordination of PLC training for educator staff, procurement of PLC units, the creation of pneumatic training kit simulators and completeness, the administration of activities and technical details . The procurement of PLC control techniques and pneumatic training kits was successfully carried out for 6 (six) months smoothly from April to October 2015. This activity was conducted on campus of DTM SV - UGM and in SMK N I Ngawen. Implementation of this training kit was conducted by PKM team and education staffs of SMK N I Ngawen Gunungkidul. The preparation of pneumatic training kits begins with the purchase and preparation of training components to the preparation of layouts and testing of training kits. The delivery of pneumatic training kits was done after the teaching staffs of SMK N I Ngawen Gunungkidul attended training kit training.

Stochastic process design kits for photonic circuits based on polynomial chaos augmented macro-modelling

Fabrication tolerances can significantly degrade the performance of fabricated photonic circuits and process yield. It is essential to include these stochastic uncertainties in the design phase in order to predict the statistical behaviour of a device before the final fabrication. This paper presents a method to build a novel class of stochastic-based building blocks for the preparation of Process Design Kits for the analysis and design of photonic circuits. The proposed design kits directly store the information on the stochastic behaviour of each building block in the form of a generalized-polynomial-chaos-based augmented macro-model obtained by properly exploiting stochastic collocation and Galerkin methods. Using these macro-models, only a single deterministic simulation is required to compute the stochastic moments of any arbitrary photonic circuit, without the need of running a large number of time-consuming circuit simulations thereby dramatically improving simulation efficiency. The effectiveness of the proposed approach is verified by means of classical photonic circuit examples with multiple uncertain variables.

Prevalence of &amp;lt;i&amp;gt;Plasmodium falciparum&amp;lt;/i&amp;gt; Among New Intakes in the Isaac Jasper Boro College of Education, Sagbama, Bayelsa State, Nigeria

This study investigated the prevalence of <i>Plasmodium falciparum</i> among new intake of the Isaac Jasper Boro College of Education, Sagbama during January-March, 2017. Blood samples were collected through venal puncture. A 2.5ml part of blood was collected from each student and transferred into an anti- coagulant bottle. Malaria parasites were determined using Pf malaria rapid test kitsanda standard microscopy. The preparation of the test kits and microscopy followed standard procedures. Determination of positive slides followed standard techniques. Data were analyzed using descriptive and inferential statistics. Malaria was positive slides were 15.49% of the total slides. <i>Plasmodium falciparum</i> accounted for 100% of all the positive cases. Male (18.75%) were more infected than female (12.82%). The difference was significant (χ²cal = 16.68, p<0.05). The prevalence of <i>P. falciparum</i> varies with the age across age group. The age bracket29-31(25.0%) had more prevalence, followed by age bracket 17-19 (17.65%). Least prevalence (9.09%) was recorded among age bracket 23-25. Differences were not significant (χ²cal = 7.96, p>0.05). Prevalence of <i>P. falciparum</i> varied across departments. The differences were statistically significant (χ²cal = 57.71, p>0.05). The result is a public health concern and call for prompt intervention.

Improving treatment times for patients with in-hospital stroke using a standardized protocol

BackgroundPrevious reports have shown significant delays in treatment of in-hospital stroke (IHS). We developed and implemented our IHS alert protocol in April 2014. We aimed to determine the influence of implementation of our IHS alert protocol. MethodsOur implementation processes comprise the following four main steps: IHS protocol development, workshops for hospital staff to learn about the protocol, preparation of standardized IHS treatment kits, and obtaining feedback in a monthly hospital staff conference. We retrospectively compared protocol metrics and clinical outcomes of patients with IHS treated with intravenous thrombolysis and/or endovascular therapy between before (January 2008–March 2014) and after implementation (April 2014–December 2016). ResultsFifty-five patients were included (pre, 25; post, 30). After the implementation, significant reductions occurred in the median time from stroke recognition to evaluation by a neurologist (30 vs. 13.5min, p<0.01) and to first neuroimaging (50 vs. 26.5min, p<0.01) and in the median time from first neuroimaging to intravenous thrombolysis (45 vs. 16min, p=0.02). The median time from first neuroimaging to endovascular therapy had a tendency to decrease (75 vs. 53min, p=0.08). There were no differences in the favorable outcomes (modified Rankin scale score of 0–2) at discharge or the incidence of symptomatic intracranial hemorrhage between the two periods. ConclusionOur IHS alert protocol implementation saved time in treating patients with IHS without compromising safety.