A novel glucose sensor was fabricated using the Langmuir–Blodgett (LB) technique with a phospholipid analogous vinyl polymer, 2-[2-methacryloyloxy)ethyldimethyl-ammonium]ethyl-5β-cholesten-3-yl phosphate polymer (PCP). The ordered monolayers of PCP were easily prepared at the interface of air–aqueous solution containing glucose oxidase (GOD), and could be transferred onto the platinum wire electrode. The response current for the observed electrode was constant for 50 days, after an initial change of signal over the first few days.