Preparation Method Research Articles

A comparison of extraction methods for preparation of glucosinolate-containing extracts from Brassica juncea antagonistic toward Ditylenchus dipsaci

Summary The stem and bulb nematode, a globally distributed pest, primarily affects onions and garlic, causing significant yield losses. Chemical treatments are a practical and effective method for controlling plant-parasitic nematodes to protect high-value crops. However, to minimise environmental damage, chemicals of natural origin need to be investigated. Brown mustard (Brassica juncea) plant is an annual plant in the Brassicaceae family, which is rich in bioactive compounds called glucosinolates. These bioactive compounds play a major role in the defence of Brassica plants against biotic stress factors in nature. Based on this point, efforts to develop plant protection products from the brown mustard plant are ongoing. In this study, the most suitable pre-extraction and extraction conditions were determined to ensure the maximum preservation of glucosinolate content and biological activity, to process brown mustard on an industrial scale. For this purpose, steam treatment was applied before extraction at 3 bar (=3 × 105 Pa) pressure for 1 and 3 min. Four different temperatures (room temperature, 40, 55 and 70°C) and two different methanol concentrations (70% and 80%) were tested to optimise glucosinolates extraction from brown mustard. It was found that sinigrin was the most abundant glucosinolate, with extraction yields 1184.4 ± 14.4 mg kg−1 at 55°C for 3 min, and 2624.2 ± 98.5 mg kg−1 at 70°C/70% MeOH for 1 min. However, nematode immobility was higher at 55°C/70% MeOH for 3 min. It was determined that the application of hot steam for 3 min at 3 bar pressure as a pre-extraction process provided optimal conditions for methanolic extraction. The results may contribute significantly to the development of potential plant protection products with further studies.

Repetitive ultramicrotome trimming and SEM imaging for characterizing printed multilayer structures

Ultramicrotomy is a well-established technique that has been applied in biology and medical research to produce thin sections or a blockface of an embedded sample for microscopy. Recently, this technique has also been applied in materials science or micro- and nanotechnology as a sample preparation method for subsequent characterization. In this work, an application of ultramicrotomy for the cross-section preparation of an inkjet-printed multilayer structure is demonstrated. The investigated device is a capacitor consisting of three layers. The top and bottom electrodes are printed with silver nanoparticle ink and the dielectric layer with a ceramic nanoparticle/polymer ink. A 3D profilometer is initially used to study the surface morphology of the printed multilayer. The measurements show that both electrodes exhibit a coffee-ring effect, which results in an inhomogeneous layer structure of the device. To obtain precise 3D information on the multilayer, cross-sections must be prepared. Argon ion beam milling is the current gold standard to produce a single cross-section in good quality, however, the cross-section position within the multilayer volume is poorly defined. Moreover, the milling process requires a significant investment of time and resources. Herein, we develop an efficient method to realize repetitive cross-section preparation at well-defined positions in the multilayer volume. Repetitive cross-sections are exposed by trimming with an ultramicrotome (UM) and this blockface is subsequently transferred into a scanning electron microscope (SEM) for imaging. A combination of custom-modified UM and SEM specimen holders allows repeated transfer of the clamped multilayer sample between instruments without damage and with high positioning accuracy. This novel approach enhances the combination of an established ultramicrotome and a SEM for multilayer sample volume investigation. Thus, a comprehensive understanding of printed multilayer structures can be gained, to derive insights for optimization of device architecture and printing process.

FraMiTrACR: A Sustainable and Economical Technology for Analytical Sample Preparation.

There are several globally recognized methods for preparing laboratory samples. Of these, the QuEChERS and QuPPe methods are commonly used for food laboratory sample preparation. As an alternative, we developed the fractionation method using FraMiTrACR. We present a life cycle assessment for the QuEChERS-, QuPPe- and FraMiTrACR methods. Our objective was to collect data to evaluate the carbon footprint of each method. However, as the ecological factors alone do not inform suitability of any given method, we also evaluated economic factors. Our life cycle assessments followed ISO 14040/44 to determine the carbon footprint of each method. Also, we have analyzed existing data to support our comparison of all three methods. The mass of consumables and packaging for our FraMiTrACR method was observed to decrease by 45% and 34% from those required for the QuPPe and QuEChERS methods, respectively. Furthermore, we calculated a 43% reduction in carbon footprint when using FraMiTrACR compared to QuPPe and a 31% reduction compared to QuEChERS. In addition, we determined that our method offers time savings >87% and >71% compared to QuEChERS and QuPPe, respectively. The main economic benefit of FraMiTrACR comes from 84% and 70% labor cost savings compared to QuEChERS and QuPPe, respectively. The laboratory using fractionation method can process 320 samples with FraMiTrACR within 8 hours, an 87% increase in potential compared to QuEChERS and a 71% increase compared to QuPPe. Fractionation using FraMiTrACR is a more sustainable method for analytical sample preparation, offering the same quality of results and far-reaching economic advantages. In comparison, FraMiTrACR uses up to 45% less consumables and packaging by weight and a reduction in kg CO2eq of up to 43%. In addition, the fractionation method offers up to 85% time savings and up to an 84% reduction in labor cost per sample.

Detection of volatile organic compounds in soils (literature review)

Volatile organic compounds (VOC) are major environmental pollutants. Due to their high mobility, they penetrate into all environmental objects, pose an environmental threat and health risks. Getting into the soil, they deteriorate its quality. VOC content requires reliable control. There is presented a review of the literature, including methods of the US Environmental Protection Agency, and regulatory and methodological documents of the Russian Federation regulating methods of selection, storage, preparation and analysis of soil samples for VOC content. The dominant place among methods for monitoring for VOCs belongs to gas chromatography with various types of detectors. For multicomponent analysis of complex objects, gas chromatography with mass selective detection is used due to the wide capabilities provided by the mass detector. A universal mass spectrometry method used in analytical laboratories is electron impact ionization mass spectrometry. For research purposes, modern highly sensitive methods are used – mass spectrometry based on the proton transfer reaction PTR-MS, ion trap mass spectrometry PIT-MS, negative ion ionization mass spectrometry NI-PT-CIMS, time-of-flight mass spectrometry of the transfer reaction proton PTR-TOF-MS. The collection and storage of samples for VOC analysis requires compliance with regulations to prevent both loss of analytes and sample contamination. Sample preparation includes methods such as vacuum and azeotropic distillation, thermal desorption, liquid extraction, various options for static and dynamic headspace analysis.

Polysaccharide-Based Composite Films: Promising Biodegradable Food Packaging Materials.

With growing concerns about environmental protection and sustainable development, the development of new biodegradable food packaging materials has become a significant focus for the future of food packaging. Polysaccharides, such as cellulose, chitosan, and starch, are considered ideal biodegradable packaging materials due to their wide availability, good biocompatibility, and biodegradability. These materials have garnered extensive attention from researchers in food packaging, leading to considerable advancements in the application of polysaccharide-based food packaging films, coatings, aerogels, and other forms. Therefore, this review focuses on the application of polysaccharide-based packaging films in food storage and preservation and discusses their preparation methods, application progress, challenges, and future development directions. Through an in-depth analysis of the existing literature, this review aims to provide sustainable and environmentally friendly solutions for the food packaging industry.

Effectively regulating Ni distribution on CeO2 through formation of Ce1−xNixO2−y solid solution to enhance the selectivity in RWGS reaction

Nickel-based catalysts frequently encounter significant challenges in achieving both high CO2 conversion and superior CO selectivity during reverse water gas shift (RWGS) reactions. To address this issue, we developed a facile strategy to prepare highly dispersed Ni/CeO2 catalysts by forming Ce1−xNixO2−y solid solutions. Under conditions of 300–600 °C, 0.1 MPa, and a gas hourly space velocity (GHSV) of 40,000 mL gcat−1 h−1, the 5Ni/CeO2-A catalyst exhibited outstanding selectivity, approaching 100 %. The RWGS reaction rate reached 1332 μmolCO gNi−1 s−1 and maintained stable for 50 h almost without deactivation. The formation of Ce1−xNixO2−y solid solution uniformly disperses numerous Ni species in the CeO2 lattice, effectively inhibiting Ni aggregation. This highly dispersed Ni weakens CO adsorption and facilitates its direct desorption without excessive hydrogenation. Moreover, the catalyst remains well-dispersed state even after reduction. The formed Ce1−xNixO2−y solid solution generates abundant oxygen vacancies on the catalyst surface, enhancing H2 dissociation. The catalyst’s pore size distribution is also modified, creating stacked pores that favor reactant diffusion and expose more active centers. In-situ DRIFTS experiments demonstrate that Ce-OH plays a pivotal role in CO2 adsorption on the 5Ni/CeO2-A catalyst, while the stabilization of bicarbonate and formate intermediates by the highly dispersed Ni further promotes catalytic performance. This study introduces a novel preparation method and provides valuable insights for designing Ni-based catalysts with exceptional CO selectivity.

Liposomes – metabolically active drug transport systems: visualization and pharmacokinetic. Part 2 (review)

Introduction. In the second part of the review we discussed aspects of visualization, pharmacokinetics and biodistribution of liposomes.Text. Many different methodsh as been proposed for the visualization of liposoms morphology and quality such as light microscopy, ESEM, TEM, AFM, etc. Each method have own advantages and limitations which are discussed in the article: In general, the selection of method depends on the specific morphological characteristics and level of details. It is important to understand the specificity of the liposomes and the visualization method for correct preparation of samples. Adequately performed pharmacokinetic and biodistribution studies can also be used as a tool for liposome visualization. The nature of active pharmaceutical ingredients, dose, lipid components, size of liposomes, charge, coating of liposomes with excipients and route of administration significantly affects the pharmacokinetics of liposomal forms. Additionally, the interaction of liposomal forms with the immune system, reticuloendothelial system and blood components play an important role in their absorption, distribution and elimination.Conclusion. The better understanding of the absorption, biodistribution, metabolism and clearance of liposomal formulations is essential for the development of modern drugs.

Strategies for Achieving Carbon Neutrality: Dual-Atom Catalysts in Focus.

Carbon neutrality is a fundamental strategy for achieving the sustainable development of human society. Catalyzing CO2 reduction into various high-value-added fuels serves as an effective pathway to achieve this strategic objective. Atom-dispersed catalysts have received extensive attention due to their maximum atomic utilization, high catalytic selectivity, and exceptional catalytic performance. Dual-atom catalysts (DACs), as an extension of single-atom catalysts (SACs), not only retain the advantages of SACs, but also produce many new properties. This review initiates its exploration by elucidating the mechanism of CO2 reduction reaction (CO2RR) from CO2 adsorption and CO2 activation. Then, a comprehensive summary of recently developed preparation methods of DACs is presented. Importantly, the mechanisms underlying the promoted catalytic performance of DACs in comparison to SACs are subjected to a comprehensive analysis from adjustable adsorption capacity, tunable electronic structure, strong synergistic effect, and enhanced spacing effect, elucidating their respective superiorities in CO2RR. Subsequently, the application of DACs in CO2RR is discussed in detail. Conclusively, the prospective trajectories and inherent challenges of CO2RR are expounded upon concerning the continued advancement of DACs. This thorough review not only enhances the comprehension of DACs within CO2RR but also accentuates the prospective developments in the design of sophisticated catalytic materials.

Hybrid Particle Size Template Method for Controllable Synthesis of Nitrogen-Doped Multilevel Porous Carbon as High-Rate Zn-Ion Hybrid Supercapacitor Cathode Materials.

Achieving high rate performance without compromising energy density has always been a critical objective for zinc-ion hybrid supercapacitors (ZHSCs). The pore structure and surface properties of carbon cathode materials play a crucial role. We propose utilizing a hybrid particle size (20 and 40 nm) magnesium oxide templates to regulate the pore structure of nitrogen-doped porous carbon derived from the soybean isolate. The multilevel pore structure enhanced ion transport efficiency while also improving the utilization of micropores. Nitrogen doping and oxygen-containing functional groups enhanced the wettability of carbon materials with aqueous electrolytes and facilitated the chemisorption of Zn2+ on the carbon material surface. The nitrogen-doped multilevel porous carbon material (HT-NMPC-1/1) prepared with a 1 : 1 mass ratio of the two templates exhibited a specific capacity of 146.65 mAh g-1 at 0.2 A g-1. Moreover, the Swagelok cells assembled with HT-NMPC-1/1 and Zn foil achieved a high energy density of 121.5 W h kg-1, high power output of 166 W kg-1, and 93.09 % capacity retention after 8000 cycles at 2 A g-1. Therefore, HT-NMPC-1/1 is a highly promising candidate for ZHSCs cathode materials. Furthermore, the novel pore regulation strategy and straightforward preparation method offer valuable reference points for other porous carbon-based functional materials.

Platan seeds-derived PSC/Ni/Co composite with multi-morphologies as tunable microwave absorber

Biomass and its derived carbon materials, due to their glorious application value in the field of microwave absorption, have sparked a new round of research boom. Hereinto, platan seeds-derived carbon-based materials (PSC), offspring from direct carbonization of platan seeds, were ulteriorly processed and subjected to high-temperature carbonization treatment to synthesis PSC/Ni/Co composite with diversified morphology and tunable microwave absorption performance. The effect of nickel and cobalt ion concentrations on the absorption performance of composite materials was investigated in depth. The optimum reflection loss value (RLmin) of PSC/Ni can reach −47.36 dB, while its effective absorption broadband (EAB) is 5.8 GHz, ranging from 12–17.8 GHz, covering almost the whole Ku band. Compared with PSC/Ni composites, PSC/Co fulfils greater advantages in thickness and bandwidth. As for PSC/Ni/Co, the sample exhibits the optimal performance when Ni and Co ions are at equal concentrations, with an RLmin of −48.3 dB and an EAB of 5 GHz (13–18 GHz), which congealed the edges of both. Unequivocally, the morphological polymorphism of carbon-based-derived microwave absorbing materials with platan seeds as the carbon source is crucial to harvset lightweight, efficient and adjustable absorption properties, let alone its relatively simple and feasible preparation method. This may provide a new approach for engineering light-weight, cost effective and eco-friendly absorbing materials.

CoFe2O4 nanoparticles encapsulated in rGO by the facile impregnation method as an efficient bifunctional electrocatalyst for alkaline water splitting

Water splitting is a promising approach for the sustainable production of green hydrogen. However, large-scale green hydrogen generation through water splitting requires highly robust electrocatalysts for the oxygen evolution reaction (OER) and hydrogen evolution reaction (HER). Herein, CoFe2O4 nanoparticles anchored on reduced graphene oxide (rGO) hybrid (CoFe2O4/rGO) was synthesized using a facile impregnation method and evaluated as a bifunctional electrocatalyst for water splitting. CoFe2O4/rGO exhibits a low overpotential of only 421 mV at 50 mA cm−2 for OER and the overpotential of 343 mV at 50 mA cm−2 for HER in alkaline electrolyte. The homogeneous distribution of CoFe2O4 nanoparticles on rGO in the CoFe2O4/rGO hybrid promotes the full exposure of plentiful active sites and facilitates rapid electron transfer during both the OER and HER. CoFe2O4 nanoparticles tightly anchored on rGO in the CoFe2O4/rGO hybrid is beneficial for improving stability for both the OER and HER. Furthermore, water splitting measurements with CoFe2O4/rGO as the cathode and anode demonstrated excellent durability for 100 h during the overall water splitting process, indicating the superior stability of CoFe2O4/rGO for both OER and HER. CoFe2O4/rGO exhibits great potential as a bifunctional electrocatalyst for green hydrogen generation through water splitting. This study provides a facile preparation method for spinel-type oxides modified graphene hybrid for water splitting.

A biomimetic phosphor that can build a rigid microenvironment for its long-lived afterglow in aqueous medium

Organic phosphorescent materials have great prospects for application, whose performance particularly depends on the preparation method. Inspired by nature’s wisdom, we report a phosphor that can utilize monomers in its environment by polymerization to construct a rigid microenvironment under light illumination, leading to a glow-in-the-dark emulsion with a phosphorescence lifetime of 1 s in water. This phosphor can achieve active growth of the aqueous emulsion with the introduction of more monomers. In the presence of trace amounts of oxygen (which has adverse effects on both polymerization and afterglow), this phosphor can still undergo photo-induced polymerization, removing the influence of oxygen and obtaining afterglow emulsion, demonstrating its adaptability to the environment. This phosphor can also catalyze the polymerization of monomers containing yellow fluorophore, obtaining long-lifetime yellow afterglow emulsion through excited state energy transfer. We have also conducted in-depth studies on the photo-catalytic and phosphorescent properties of this phosphor in model systems. This biomimetic intelligent manufacturing provides a new approach for organic phosphorescent materials and is significant for future applications.

Towards valorization of glycerol and molasses: Carbon-based catalysts from molasses for the synthesis of acetins

Crude sugarcane molasses (SCM) was successfully applied for the first time as a bio-feedstock for producing biochar catalysts for glycerol upgrading. Preparation methods were developed, including partial or hydrothermal carbonization (abbr. PC and HTC) and chemical activation. After functionalization with −SO3H groups, the catalysts were tested for the esterification of glycerol to acetins. The materials varied in their textural and chemical features, particularly in the −SO3H content, giving the single-step PC method a distinct advantage. The best catalyst yielded approximately 74 % of di- and tri-acetins with 97 % glycerol conversion within only 2 h of the reaction and demonstrated great stability over three consecutive cycles. The formation of the desired TA product was correlated with the concentration of −SO3H groups. Despite being non-porous, the most active PC catalyst possessed a compact structure with a high abundance and easy accessibility of −SO3H, –COOH, and –OH groups on its surface.

Mushroom production on digestate: Mineral composition of cultivation compost, mushrooms, spent mushroom compost and spent casing

Produced in the process of anaerobic digestion, the effluent called digestate is rich in nutrients and can be used as a growing media for mushrooms. However, it can also be rich in non-essential and trace elements, heavy metals, various organic pollutants, pharmaceuticals, and other unwanted compounds with potential negative effects. Therefore, two button mushroom species, Agaricus bisporus (brown cultivar) and Agaricus subrufescens, were cultivated on digestate based substrate. The mineral composition of the experimental mushroom compost (EMC), mushrooms (M), spent mushroom compost (SMC) and spent casing (SC) was evaluated by means of ICP OES. Mineral distribution and quantity were substrate dependent, digestate origin was determined for most of investigated elements, excluding Ca, Mo, Pb, Ce and Nd, where the source was straw. However, content of elements with high mobility such as Cd, Cu, Pb and Zn for EMC was low. Short composting method for mushroom compost preparation used in this study could be suitable method for reducing available Cd, Cr, Ni, Pb, Zn and total As. For the casing material, bark was richer in major essential elements (MEE's) and essential trace elements (ETE's), besides Ca, where peat indicated higher content (1490 mg kg-1). From the trace elements with detrimental health effects (TEWDHE) group, bark was richer in Ba and Pb, but peat contained significant content of As (3.92 mg kg-1). The results clearly indicated both the studied mushrooms are valuable source of K, Na and Se, while A. subrufescens provided higher amounts of Cu and Zn. No threat for human consumption for Ni, Pb, As, and Cd, their content is under the limits and decreases with each subsequent mushroom yield. SMC and SC were nutrient rich especially for Fe, Mg, Mn, Si and Zn, giving them added value as biobased product for boosting vegetable crop yield. However, Cr and Ni, ETS's for plants in lower amounts, were elevated in SMC/SC, therefore the mineral composition should be monitored. Low concentration of hazardous elements in the spent substrates allows for subsequent use.

Application of PACz-Based Self-Assembled Monolayer Materials in Efficient Perovskite Solar Cells.

Due to the advantages of low interface resistance, high work function, and high stability, PACz family materials have developed rapidly in p-i-n structure perovskite solar cells (PSCs) in recent years. Numerous studies have shown that PSCs prepared on the basis of PACz family materials or their derivatives as hole transport layers (HTLs) generally exhibit superior performance compared to PSCs prepared on the basis of organic HTL PTAA and inorganic HTL NiOx, especially in terms of stability, demonstrating unparalleled charm. Since the application of PACz-like molecule V1036 as a HTL in PSCs in 2018, research reports on high-performance PSCs based on the PACz family HTL have been widely disseminated. Currently, PSCs based on the PACz HTL exhibit a world record value of 26.7% power conversion efficiency (PCE), breaking the long-standing world record held by n-i-p-structured PSCs, indicating its great potential for application in PSCs. The main problem with using PACz as a HTL is that it exhibits poor wettability toward perovskite precursor solutions, is sensitive to the thickness of the HTL and the roughness of the substrate, has poor thermal stability, and lacks preparation methods, making it difficult to achieve large-area device fabrication. This review summarizes the outstanding achievements of PACz to date, describes the mechanism and unique properties of PACz in PSCs, and looks forward to the future development prospects of PACz.

Hair and Saliva Endocannabinoid and Steroid Hormone Analysis by Liquid Chromatography Paired with Tandem Mass Spectrometry.

Endocannabinoids are lipid neurotransmitters that play an important part in human health. Recent methods have found that quantification of endocannabinoids in hair and saliva samples is possible using liquid chromatography paired with tandem mass spectrometry (LC-MS/MS). This chapter describes two simple sample preparation methods that can be used to prepare hair and saliva samples for analysis using LC-MS/MS. Our LC-MS/MS method can be applied to both hair and saliva samples and is sufficiently sensitive for endocannabinoid, as well as steroid hormone, quantification in both of these sample matrices. This chapter provides a comprehensive description of how this can be achieved and provides tips and tricks for troubleshooting problems users may experience.

Physicochemical and Structural Properties of Resistant Starch Prepared from Tacca Tuber Starch using Autoclaving-Cooling and Acid Hydrolysis Treatments

Tacca tuber (Tacca leontopetaloides) is a neglected and underutilized plant species. Recently, tacca tuber has been studied as a food plant source of high-amylose starch, so it has the potential to be developed into resistant starch (RS), especially type-3 RS (RS3). RS3 can be prepared through the autoclave-cooling (AC) process, but the RS produced is not optimal. Further increase in RS can be obtained through hydrolysis of citric acid (AH). Until now, systematic knowledge about the preparation method and characterization of RS3 produced from tacca tuber starch through the AC-AH process remains unavailable. This study aims to determine the physicochemical properties and structure of RS3 from tacca tuber starch made through the AC-AH process. Tacca tubers were obtained from the Garut coast, Indonesia. They were first extracted to obtain starch and then processed using the AC process to make RS3. The acquired RS3 was hydrolyzed using AH at various concentrations and hydrolysis times. The results showed that all treatments did not affect the proximate composition, except for water content. Significant effects were observed on amylose and RS levels. The swelling power, solubility, water holding capacity, oil holding capacity and color profile of RS3 also significantly changed. The morphology of RS3 was deformed, accompanied by a change in its crystal structure to a combination of B and V types. This alteration was followed by an increase in the relative crystallinity and thermal profile, while the paste profile decreased significantly. In conclusion, all preparation methods affected the physicochemical properties and structure of RS3 from yam starch, except for the proximate composition. AC-AH treatment with 0.3 M citric acid for 4 h is recommended for preparing RS3 because it produces the highest RS content. The acquired RS3 has the potential to be applied in products that require good paste stability, and have health benefits. HIGHLIGHTS Tacca tuber is a neglected and underutilized plant species, a potential source of high amylose starch to be developed into resistant starch type-3 (RS3). Autoclaving-cooling (AC) and citric acid hydrolysis (AH) treatments produced 32.40 % RS3. RS3 has an irregular, rough and agglomerate morphological structure. The crystal structure of RS3 is a combination of types B and V, with a relative crystallinity of 24.38 - 45.94 %. AC-AH treatment with 0.3 M citric acid for 4 h is recommended in preparing RS3 because it results in the highest RS content. GRAPHICAL ABSTRACT

Sulfur dioxide gas sensor based on vanadium oxide doped TiO2 nanopaper

Abstract The gas sensor based on TiO2 nanomaterials is promising for both industry and daily life because of its simple fabrication, low cost, high sensitivity, and easy application to micro-devices. However, its selectivity is relatively low and strongly influenced by the environment, thus limiting its practical application. In this work, we prepared TiO2 nanopaper by methods of hydrothermal synthesis and conventional paper preparation. To improve the selectivity, we added V2O5 by immersing the as-prepared nanopaper in an ammonium metavanadate (NH4VO3) solution evenly dispersed in ethanol. Nanopaper is a random array of long nanowires and nanofibers, which retains its shape and structure at high temperature, unlike pure nanowires, due to its high porosity and mechanical stability. V2O5 leads to a selective sensing performance with high catalytic activity for SO2 gas. The surface structure of the sensing material and its porosity were characterized by SEM, XRD, XPS. The improved sensing material exhibited a high response of about 22.6 for 100 ppm SO2 and a fast response and recovery of 9 s and 14 s, respectively. It also exhibited good reproducibility and selectivity in other interfering gases. Furthermore, the long-term sensing performance in the atmospheric environment was maintained for about 50 days.

Integrative Multi-PTM Proteomics Reveals Dynamic Global, Redox, Phosphorylation, and Acetylation Regulation in Cytokine-Treated Pancreatic Beta Cells

Studying regulation of protein function at a systems level necessitates an understanding of the interplay among diverse post-translational modifications (PTMs). A variety of proteomics sample processing workflows are currently used to study specific PTMs but rarely characterize multiple types of PTMs from the same sample inputs. Method incompatibilities and laborious sample preparation steps complicate large-scale physiological investigations and can lead to variations in results. The single-pot, solid-phase-enhanced sample preparation (SP3) method for sample cleanup is compatible with different lysis buffers and amenable to automation, making it attractive for high-throughput multi-PTM profiling. Herein, we describe an integrative SP3 workflow for multiplexed quantification of protein abundance, cysteine thiol oxidation, phosphorylation, and acetylation. The broad applicability of this approach is demonstrated using cell and tissue samples, and its utility for studying interacting regulatory networks is highlighted in a time-course experiment of cytokine-treated β-cells. We observed a swift response in global regulation of protein abundances consistent with rapid activation of JAK-STAT and NF-κB signaling pathways. Regulators of these pathways as well as proteins involved in their target processes displayed multi-PTM dynamics indicative of a complex cellular response stages: acute, adaptation, and chronic (prolonged stress). PARP14, a negative regulator of JAK-STAT, had multiple co-localized PTMs that may be involved in intraprotein regulatory crosstalk. Our workflow provides a high-throughput platform that can profile multi-PTMomes from the same sample set, which is valuable in unraveling the functional roles of PTMs and their co-regulation.

Formulation and Evaluation of Herbal Lipstick

A considerable amount of literature has been published on several aspects of lipsticks Production. To date, there is no collation of studies related to lipsticks production that has been published. This review was conducted to examine information about the history of lipsticks; ingredients Used in the Preparation of lipsticks, focusing on the natural and chemical ingredients; methods of Preparation for the Lipsticks; and the characterization of the lipsticks. A literature search for English Language articles was Conducted by searching electronic databases including Web of Science, Scopus, PubMed, and Google Scholar. Overall, the evidence indicates that lipsticks have been used since Ancient times and are among The highest demand cosmetics. The findings of this review summarize Those of earlier studies that explained The use of different types of ingredients in the manufacturing Processes of lipsticks. It highlights the Importance of using green technology and ingredients..