Use Of Preparations Research Articles

COMPLEX METHOD FOR AUTOMATIC RECOGNITION OF NATURAL LANGUAGE AND EMOTIONAL STATE

Current trends in NLP emphasize universal models and learning from pre-trained models. This article explores these trends and advanced models of pre-service learning. Inputs are converted into words or contextual embeddings that serve as inputs to encoders and decoders. The corpus of the author's publications over the past six years is used as the object of the research. The main methods of research are the analysis of scientific literature, prototyping, and experimental use of systems in the direction of research. Speech recognition players are divided into players with huge computing resources for whom training on large unlabeled data is a common procedure and players who are focused on training small local speech recognition models on pre-labeled audio data due to a lack of resources. Approaches and frameworks for working with unlabeled data and limited computing resources are almost not present, and methods based on iterative training are not developed and require scientific efforts for development. The research aims to develop methods of iterative training on unlabeled audio data to obtain productively ready speech recognition models with greater accuracy and limited resources. A separate block proposes methods of data preparation for use in training speech recognition systems and a pipeline for automatic training of speech recognition systems using pseudo marking of audio data. The prototype and solution of a real business problem of emotion detection demonstrate the capabilities and limitations of owl recognition systems and emotional states. With the use of the proposed methods of pseudo-labeling, it is possible to obtain recognition accuracy close to the market leaders without significant investment in computing resources, and for languages with a small amount of open data, it can even be surpassed.

Geological characteristics of the limestone deposit "Dobrilovići"-Loznica and its preparation for use in agriculture

Geological characteristics of the limestone deposit "Dobrilovići"-Loznica and its preparation for use in agriculture

ОБҐРУНТУВАННЯ НЕОБХІДНОСТІ СТВОРЕННЯ МОБІЛЬНОГО КОМПЛЕКСУ УПРАВЛІННЯ БЕЗПІЛОТНИМИ ЛІТАЛЬНИМИ АПАРАТАМИ НА БАЗІ ШАСІ ВАНТАЖНОГО АВТОМОБІЛЯ

The conducted SWOT analysis made it possible to develop problematic issues that are proposed during the operation of unmanned aerial vehicles, proposals were made for the creation of a mobile complex of the use of unmanned aerial vehicles based on the chassis of a truck. Formulation of the problem. During hostilities, unmanned aerial vehicles (UAVs) were widely used. The massive use of the latest robotic (automated) weapons and military equipment on the battlefield is changing the nature of units' actions. Currently, almost every combat unit has a UAV application group. The high efficiency of their use is confirmed by high results on the battlefield. The security and defense forces of Ukraine are armed with dozens of types of UAVs for various purposes and with various capabilities for lifting cargo and carrying explosives. The use of UAV combat units in the field. Launches of lethal vehicles are carried out by hand or with the help of launch devices. With this mobile application group, the BPLA, although it is at a distance from the line of the next combat encounter, does not have sufficient security and protection from enemy fire. Preparation for use, maintenance of UAVs and charging of batteries in stationary conditions the day before, which reduces the efficiency of actions.The deployment of mobile groups of UAV operators is carried out on vehicles that are available to the military unit at the time, and which are not adapted for the high-quality performance of the combat mission by the group. UAV operators and equipment are not protected from exposure to low ambient temperatures and have no protection from small arms and shrapnel damage. Time indicators of deployment on the terrain of the complex for launching UAVs and folding after the completion of a combat mission need improvement. Analysis of recent research and publications. Scientific studies on improving the actions of units equipped with UAVs were carried out in the National Guard even before the beginning of the full-scale invasion of the Russian Federation. For example, in [1-2], data are given regarding the need for intelligence information and the development of a rational procedure for the use of intelligence unmanned aerial vehicles. In [3], the methodological apparatus for researching the problems of using information technologies and telecommunication systems in the process of military management is presented. The purpose of the article is to justify the need to create a mobile complex for the use of unmanned aerial vehicles based on a truck chassis. Provide recommendations on the creation of a mobile complex for the use of unmanned aerial vehicles.

Analysis of metamorphism and tendency of coal seams and their hazardous properties

The object of this study is coals of different stages of metamorphism. Currently, a situation has arisen when indicators developed to establish the consumer qualities of coal are used to predict the manifestation of hazardous properties of mine seams during mining operations. The need to consider the fuel for its working condition is due to different end goals between the establishment of consumer qualities of coal and the manifestation of the hazardous properties of mine seams. The condition and quality of coal after its preparation for use is significantly different from the condition in the mining area. Appropriate sample preparation changes the physical and chemical properties of coals, which determine the manifestation of the hazardous properties of mine layers during mining. To eliminate such a discrepancy, the quality indicators of coals were recalculated for their working condition, taking into account the yield of ash and moisture content for the mined mine seams, followed by an analysis of changes in the correlations be-tween the indicators. The indicators of the manifestation of the hazardous proper-ties of mine layers are borrowed from the characteristics of the quality of the fuel, reduced to a dry, ash-free state. Using these indicators, additional errors are introduced in advance into the accuracy of the prediction of the manifestation of hazardous properties during mining operations. The values of the indicators of the organic (combustible) part of the fuel serve as general reliable characteristics of its quality for the entire set of mine seams, but they cannot be used to predict the hazardous properties of a particular mine seam due to a decrease in the accuracy of their determination due to the unpredictable content of mineral impurities and moisture. The initial experimental data, which have been accumulated over several decades based on the experience of using coal for industrial purposes, are analyzed. On the basis of the conducted researches the peculiarities of the choice of indicators of metamorphic transformations of coal, which are used in parallel respectively to establish the quality of fuel and forecast the dangerous properties of coal seams, have been established. The discrepancies between the indicators of the degree of metamorphism used in the current regulatory framework for the safe conduct of mining operations, the state of fuel during mining operations in underground conditions.

Эффективность влияния микроудобрений на ростовые процессы и декоративные качества бархатцев отклоненных махровых сорта Дюранго

The use of micro-fertilizing preparations Energoshance and Polishance on marigolds of rejected terry Durango varieties in the conditions of vegetation experience was investigated. Experimental data indicate an improvement in germination and germination energy, decorative qualities of marigolds under the influence of organomineral micronutrients. The growth-stimulating effect of Energy balance and Polishance on the studied qualities of marigolds is shown, which allows us to recommend these preparations for use in landscaping urban areas of Yelets in order to achieve higher decorative qualities of plants, as well as to enhance growth processes. Keywords: ORGANOMINERAL MICRO FERTILIZERS, ENERGY BALANCE, POLISHANCE, MARIGOLDS, DECORATIVE QUALITIES, GROWTH PROCESSES, GROWTH-STIMULATING EFFECT, YELETS

TO THE QUESTION OF SCIENTIFIC SUBSTANTIATION OF THE CHOICE OF INDICATORS OF MANIFESTATION OF HAZARDOUS PROPERTIES OF COAL SEAMS

The article recalculates the quality indicators of coal for their working condition, taking into account the yield of ash and moisture content for coal seams, followed by an analysis of changes in the correlations between the indicators. The need to consider the fuel for its working condition is due to different end goals between establishing the consumer qualities of coal and the manifestation of the hazardous properties of coal seams. Different ultimate goals of the problems under consideration imply different ways of solving them, including differences between the influencing factors and their parameters. The methodology provides for the achievement of the goal to use the initial experimental data that have been accumulated over several decades based on the experience of using coal for industrial purposes. The condition and quality of coal after its preparation for use differs significantly from the condition in the mining area. These differences are associated with the preliminary enrichment of coal and the removal of a certain amount of mineral impurities. Based on conducted research out, the features of the choice of indicators of metamorphic transformations of coals were established, which are used in parallel, respectively, to establish the quality of fuel and predict the hazardous properties of coal seams. Discrepancies between the indicators of the degree of metamorphism used in the current regulatory framework for the safe conduct of mining operations and the state of fuel during mining operations in underground conditions are revealed. To improve normative documents on safe mining operations, it is necessary to consider indicators that characterize not only the organic (combustible) part of the fuel, but also the presence of mineral impurities and moisture in coals in the mining area. Keywords: coal, metamorphism, indicators, justification, mineral impurities, ratio, condition, dry, ashless, working, coal seams, mining, safety, regulatory framework, improvement.

Cultural and ethnobotanical legacy of native potatoes in Colombia

BackgroundNative potatoes are Andean tubers of great historical, social, food, genetic and nutritional importance, and they contribute significantly to food security by supplementing the household diet and also providing alternative income. Even when their cultivation and consumption imply great benefits, their use and local preservation depend to a large extent on the recognition of their ethnobotanical and cultural importance. In this context, this study consolidates an important ethnobotanical research bases for native potatoes in Colombia.MethodsThe study collected data through semi-structured interviews and dialogues (130) in the municipality of Chiscas, department of Boyacá, central-eastern Colombia. The questionnaire was focused on native potatoes and sought to investigate the knowledge related to cultivation, diversity, patterns and forms of preparation for use and consumption. Likewise, knowledge heritability mechanisms were investigated and ethnobotanical indices of relative importance, use and culture were estimated.ResultsDocumentation of ethnobotanical knowledge included aspects such as seed care and availability, cultural management of the crop, patterns of use and consumption, as well as ways of preparing the tubers. In total, 23 vernacular names of native potato and 360 reports of use (commercial, domestic or ritual-magical) were recorded for the 15 main genotypes. Quantitative estimates included the importance index: (a) cultural, for which values ranged between 0.059 and 0.812; (b) relative, with records between 0.04 and 0.43; and (c) use, which ranged between 0.06 and 0.63. The ethnobotanical importance index (d) for native potatoes was 57.26, which corresponds to a “very high” ethnobotanical value. This allowed us to identify that Criollas were the most recognized and used potatoes within the community. In addition, it was shown that vertical transmission is the main way in which traditional knowledge about native potatoes is inherited. Finally, an artificial intelligence tool was preliminarily implemented to identify the polarity generated in the interviewees by the questions.ConclusionThe results of this research provide valuable information on the ethnobotany of native potatoes in Colombia. The genotypes used by the community of the municipality of Chiscas were recognized for their high gastronomic and nutritional potential, as well as for their great ethnobotanical and cultural importance. These data can be considered as a valuable tool to support any action aimed at the conservation and revaluation of these tubers.

313.5: Meeting Packed Red Blood Cell Requirement for the Transmedics Organ Care System (OCS) Liver Transplantation Trial (PROTECT Trial) At Henry Ford Hospital

Introduction: In January 2016, HFHS Transplant Institute began the PROSPECT trial, an interventional trial studying normothermic preservation of the donated liver before transplant using the Liver Organ Care System (OCSTM). The Henry Ford Hospital Transfusion Service (TS) provided the required packed red blood cells (PRBC). Background: The TransMedics (OCSTM) liver trial was an international, randomized trial studying the intervention of a portable liver system to preserve donor livers for transplant. The system utilizes an oxygenated blood-based perfusate mixed with nutrient solution in normothermic perfusion for organ preservation. The TS provided PRBC needed for the study, final PRBC used dictated by the condition and size of the retrieved liver. TS study goal was to secure proper transfusion needs for liver perfusion while also adhering to blood inventory management. Methods: Organ procurement candidates for the PROTECT trial were selected based on pre-determined conditions and consented according to that protocol. Once the patient was consented and the liver transplantation was scheduled, the PROTECT trial team notified the TS,and 5 units of group O Positive, leukoreduced PRBC less than 10 days old were selected, and placed inside a temperature-controlled packaging container along with a remote temperature monitoring device. The container was then transported with the procurement team to the harvesting location. Once the insulated container was returned to the TS, the temperature-monitoring device information was uploaded to verify that the returned PRBC have been stored within their proper temperature limits. Results: The TS support for the PROTECT trial at HFHS occurred from January 2016 until September 2021. The table below shows the activity related to this trial. - Issued Utilized Returned Wasted PRBC units 240 132 104 4 In summary, 29/48 study events used 4 units; 3/48 study events used 5 units; on 15/48 events the organ harvesting was cancelled; all units returned had an acceptable temperature range and returned to the available inventory. For one study event, 4 PRBC were spiked in preparation for use in the perfusate prior to the event being cancelled. These PRBC were discarded and deemed wasted. Conclusions: During the PROTECT trial no PRBCs were wasted due to inappropriate storage conditions. The joint use of a temperature-controlled container and remote temperature monitoring device allowed for the appropriate return of unused PRBCs to the TS blood inventory, thus avoiding any wastage. Only 3/48 events used 5 units, the\us issuing 4 PRBC to meet the OCS™ support may suffice, further decreasing the risk for wastage of any PRBC units. After receiving FDA in September 2021, alternatives to maintain the required PRBC temperature should be addressed to minimize wastage at this time of limited blood availability for the support of the TransMedics Organ Care System (OCSTM) for liver organ perfusion.

Influence of selected mineral additives on properties of recycled aggregate concrete (RAC) considering eco-efficiency coefficients

The paper is focused on the eco-efficiency calculations of recycled aggregate concrete (RAC) consisted of ordinary Portland and blast-furnace slag cements with three fine-grained wastes: quartz powder (Q), limestone powder (L) and fly ash (F). The characteristics of concrete mixes (slump and air content) and hardened concrete properties: 28- and 90-day compressive strength, water absorption as well as the depth of the carbonation (after the accelerated carbonation in the chamber) were examined. In order to parameterize the third feature of contemporary concrete, which is (next to the strength and durability) its sustainability potential, the binder intensity (bi) and carbon intensity (ci) indexes were calculated, taking into account CO2 emissions from cement production and technological processes related to preparation for use of other ingredients. The comparison of the effect of limestone powder and fly ash showed some advantages of the fly ash (taking into account the results of strength tests). However, limestone powder turned out to be more favorable in influencing other properties (especially resistance to carbonation). Nevertheless, the use of fly ash proved to be the most effective in terms of eco-efficiency factors. The pozzolanic properties of F increased the strength of the concrete, reducing both bi and ci values. Furthermore, the presence of F canceled out the negative effect of quartz powder. The use of F together with cement containing ground-granulated blast-furnace slag (GGBS) was most beneficial. Although quartz powder due to technological reasons occurred to be the worst option, its use may be beneficial when CO2 sequestration by concrete carbonation is taken into account. Lower strength and resistance to carbonation of concrete with Q allow CO2 to penetrate deeper into the structure, especially in concrete made of GGBS cement. In this way, the later uptake of CO2 from the atmosphere allows for a partial reduction of the ci value, making RAC more eco-efficient.

Safety and efficacy of a feed additive consisting of Solanum glaucophyllum leaf extract for dairy cows and other dairy ruminants (Herbonis Animal Health Gmbh).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of Solanum glaucophyllum leaf extract (SGE) as a nutritional additive for dairy cows and other dairy ruminants. However, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) considered the glycosides of 1,25‐dihydroxycholecalciferol (1,25[OH]2D3) as the active substance and the bolus containing SGE‐derived 1,25[OH]2D3 as the preparation of the additive. The product is intended to be administered to dairy ruminants during the pre‐parturient (period from 9 days before calving to immediately before calving). The FEEDAP Panel concluded that the administration of one bolus, the preparation of the additive as applied in the animal studies evaluated, containing 500 μg of SGE‐derived 1,25[OH]2D3 during the pre‐parturient period is safe for cows. Owing to the lack of data, the Panel could not conclude on the safety for of a subsequent administration of a second bolus or on the safety of another SGE‐derived 1,25[OH]2D3 preparation for use in dairy ruminants other than cows (Bos taurus). The Panel considered that, under the specified conditions of use, the product is safe for the consumer and the environment. The bolus, a preparation containing SGE, as a source of the active substance, is not irritating to skin and eyes and it is not a sensitiser. Exposure via inhalation is unlikely. The Panel concluded that the administration of the bolus, the preparation of the additive as applied in the animal studies evaluated, containing 500 μg of SGE‐derived 1,25[OH]2D3 in a period from 9 days before calving to immediately before calving has the potential to prevent hypocalcaemia in dairy cows. Owing to the lack of data with another preparation, the Panel could not conclude on the efficacy in other dairy ruminants.

A roadmap for hydrogel-based quasi-solid electrolyte preparation for use in dye-sensitized solar cell

Although the use of quasi-solid electrolytes in dye-sensitized solar cells (DSSCs) is viewed as a solution to the stability problem of DSSCs, the network structure effect on the DSSCs’ performance has not been studied in detail. Structurally different hydrogels poly (2-methacryloyloxyethyl trimethylammonium chloride-co-acrylic acid) were prepared and used as quasi-solid gel electrolytes in DSSCs to investigate the network structure effect of hydrogels on DSSC performance. The hydrogel's network structure was changed using different amounts of cross-linkers during synthesis. Five quasi-solid electrolytes were prepared from the hydrogels. The increased cross-linking of quasi-solid electrolytes during synthesis raised conductivity from 0.102 to 0.144 S m−1. Despite expectations, it was observed that the energy conversion efficiency of DSSCs does not follow the rising trend in conductivity, as it rises from 6.57% to 6.62% and then decreases to 5.17%, with increased cross-linking. Moreover, cross-linking during hydrogel synthesis defines not only the electrical properties of quasi-solid electrolytes but also the swelling, wetting, and morphological properties of the structure. Therefore, this study aimed to investigate how and which parameters affect the energy conversion efficiency of DSSCs that are prepared with quasi-solid electrolytes.

Formation Mechanism of Carbon-Supported Hollow PtNi Nanoparticles via One-Step Preparations for Use in the Oxygen Reduction Reaction

Hollow Pt-based nanoparticles are known to possess the properties of high electrocatalytic activity and durability. Nonetheless, their practical applications as catalytic materials are limited because of the requirement for exhaustive preparation. In this study, we prepared carbon-supported hollow PtNix (x = the moles of the Ni precursor to the Pt precursor in the catalyst preparation step) catalysts using a one-step preparation method, which substantially reduced the complexity of the conventional method for preparing hollow Pt-based catalysts. In particular, this hollow structure formation mechanism was proposed based on extensive characterizations. The prepared catalysts were examined to determine if they could be used as electrocatalysts for the oxygen reduction reaction (ORR). Among the investigated catalysts, the acid-treated hollow PtNi3/C catalyst demonstrated the best ORR activity, which was 3 times higher and 2.3 times higher than those of the commercial Pt/C and acid-treated particulate PtNi3/C catalysts, respectively.

International collaborative study to evaluate and calibrate two recombinant L chain Ferritin preparations for use as a WHO International Standard.

To evaluate and calibrate two candidate preparations for the 4th International Standard for Ferritin (Human, Recombinant) (codes: 19/118 and 19/162) against the 3rd International Standard for Ferritin (Human, Recombinant) (code: 94/572), and three serum commutability samples in an international collaborative study involving 12 laboratories in nine countries. Eleven of the 12 participating laboratories performed Ferritin quantitation using automated assay platforms and one laboratory used a manual ELISA kit. There was better overall agreement between all laboratories and between assay methods for the potency of preparation 19/118 than for preparation 19/162. The overall geometric mean potency (from all methods) of the candidate 4th International Standard, 19/118, was 10.5 µg/ampoule, with inter-laboratory variability, expressed as % geometric coefficient of variation (GCV), of 4.7%. Accelerated stability studies have predicted both 19/118 and 19/162 to be very stable for long term storage at-20°C. The candidate 4th International Standard for Ferritin (Human, Recombinant) (19/118) has been shown to be immunologically similar to the 3rd International Standard for Ferritin (Human, Recombinant) (94/572). It was recommended to and accepted by the WHO Expert Committee on Biological Standardization that 19/118 be established as the 4th International Standard for Ferritin (Human, Recombinant) with an assigned potency of 10.5µg/ampoule and expanded uncertainty limits 10.2-10.8µg/ampoule (95% confidence; k=2.23).

“It’ll Never Be Safe, But You Can Limit the Harms”. Exploring Adolescents’ Strategies to Reduce Harms Associated with Psychoactive Substance Use

Introduction: Harm reduction strategies are behaviors that may be applied in order to reduce adverse consequences associated with psychoactive substance use. Adolescents who use substances make up a large population that may benefit from applying such strategies. However, previous research in this area has focused on adults. Therefore, the purpose of this qualitative study was to describe and examine harm reduction strategies among adolescents who use psychoactive substances. Methods: Semi-structured individual interviews were conducted with 72 adolescents of equal sex distribution (aged 17–18 years) who had used various substances at least three times in the past 12 months and had no history of substance use disorders (SUD) treatment. Data were transcribed and then analyzed thematically based on the Consensual Qualitative Research approach. Findings: Adolescents who use substances described the harm reduction strategies they use. According to the meaning youth attributed to these strategies, five themes were developed, that included approaches to risk, substance use safety conditions, preparation for use, manner of use, and controlled use. Most of the identified strategies correspond to those found in studies on adults. The main source of adolescents’ knowledge about these strategies was the Internet. Peers appeared to play an important role in reducing substance-related risks. Conclusions: Despite risk-taking, adolescents in our study showed active engagement in reducing their substance-related risks and harms. Practical implications are provided, including supporting harm reduction strategies, enhancing the active role of peers and the Internet as communication channels in preventing SUDs as well as other substance-related problems among adolescents.

AACE2021-A-1022: Management of Adrenal Insufficiency: Survey of Physicians’ from the Middle East and North Africa

Treatment of adrenal insufficiency (AI) requires correct lifelong use of glucocorticoids with early dose adjustments to cover the increased demand in stress to avoid life-threatening emergencies. We aimed to determine physicians' knowledge on the pathophysiological and clinical aspects of AI in the two regions of North Africa and the Middle East. An electronic questionnaire on physiology, pharmacology, and clinical management of AI of 96 participants. Half of the participants were attending physicians, and 44,.2% practiced adult endocrinology. Knowledge of physiology and pharmacology of GC hormones (e.g., normal, daily cortisol production and half-life of hydrocortisone (HC) was variable. Physicians' patterns in prescribing and monitoring the adequacy of glucocorticoid (GC) replacement therapy revealed that the majority (81.3%) identified HC preparations for use in GC replacement therapy, followed by prednisone/prednisolone (46.9%), longer-acting prednisolone formulations (13.5%), and Dexamethasone (13.5%). The most frequently reported sign for GC under-replacement was “nausea” (84.4%), followed by “weight loss” (81.3%), “headaches” (44.8%) and “sleep disturbances” (36.5%). The most frequently reported for GC over-replacement was “high blood pressure” (91.6%), followed closely by “weight gain” (90.5%) and “increased blood glucose” (86.3%). A few, 6.3% and 3.2% of respondents considered “weight loss” and “low blood pressure” as symptoms of GC over-replacement, respectively. The situations most frequently identified as necessary for adjustments of GC dosages were "acute severe disease" (92.7%) and "fever more than 38°C" (79.2%) during acute severe disease, dose increase 2-5-fold by 77.1% of respondents. Strangely, some respondents reported dose reduction or even omission. The majority (80.9%) identified the need for emergency, standby medications for patients with AI. Additionally, 94.7% reported the need to issue an emergency card, and 80.9% reported the need for relative and friends' education regarding AI. When traveling, packing adequate GC preparations would be recommended by 90.5%, emergency card (89.5%), and emergency medications (70.5%). In the investigated settings, physicians’ knowledge of physiology and pharmacology of glucocorticoids, medical replacement strategies in AI, and prevention of adrenal crisis may be insufficient. There is a need for more education and training on AI in both medical and endocrine forums.

Sugar transporters of the SWEET family and their role in arbuscular mycorrhiza.

Plant sugar transporters play an essential role in the organism’s productivity by carrying out carbohydrate transportation from source cells in the leaves to sink cells in the cortex. In addition, they aid in the regulation of a substantial part of the exchange of nutrients with microorganisms in the rhizosphere (bacteria and fungi), an ty essential to the formation of symbiotic relationships. This review pays special attention to carbohydrate nutrition during the development of arbuscular mycorrhiza (AM), a symbiosis of plants with fungi from the Glomeromycotina subdivision. This relationship results in the host plant receiving micronutrients from the mycosymbiont, mainly phosphorus, and the fungus receiving carbon assimilation products in return. While the eff icient nutrient transport pathways in AM symbiosis are yet to be discovered, SWEET sugar transporters are one of the three key families of plant carbohydrate transporters. Specif ic AM symbiosis transporters can be identif ied among the SWEET proteins. The survey provides data on the study history, structure and localization, phylogeny and functions of the SWEET proteins. A high variability of both the SWEET proteins themselves and their functions is noted along with the fact that the same proteins may perform different functions in different plants. A special role is given to the SWEET transporters in AM development. SWEET transporters can also play a key role in abiotic stress tolerance, thus allowing plants to adapt to adverse environmental conditions. The development of knowledge about symbiotic systems will contribute to the creation of microbial preparations for use in agriculture in the Russian Federation.

German Translation, Linguistic Validation, and Cultural Adaptation of the Environmental Audit Tool-High Care.

Background:In dementia-specific care, the design of the environment is regarded as an influential element in the support and maintenance of skills and can improve the quality of life of residents. To date, there is no valid instrument in the German-speaking countries with which the quality of the physical environment in residential long-term care facilities can be systematically assessed.Objective:To report the translation, linguistic validation, cultural adaptation, and content validity evaluation of the Australian Environmental Audit Tool—High Care in preparation for use in German nursing homes.Method:The procedure was guided by an adapted multistep process of the World Health Organization (1998) and included focus groups involving potential users of the new tool such as scientific experts and healthcare professionals (n = 40). Content validity indices were calculated following a two-step expert survey.Results:The final draft versions of the German Environmental Audit Tool (G-EAT) included 74 and 77 items, for non-secured units and secure units, respectively, divided into 10 key design principles according to the Australian original. The evaluation of content validity showed that cultural differences existed in several items.Conclusions:The G-EAT provides the means for conducting a valid assessment of the environmental quality of people with dementia in German nursing homes. However, its usability in healthcare research must be preceded by testing its interrater reliability.

Technologies and technical means of production of complete feed and PMVA for agricultural animals

Russian market of complete feed recent years shows a steady development. Currently the Kurgan region the state of base of feed and a shortage of specialized feed additives leads to a violation of maintenance conditions for livestock, reduced productivity. The increase in production of concentrates and complete feed currently is an actual scientific and technical problem whose solution is of great scientific and practical importance. To solve this problem, the technology of production of animal feed and PMVA on the basis of full-fat extruded soybeans produced using a shaft cooler and sapropel dehydrated with the use of filtering centrifuges continuous action has been proposed. The proposed technology of production of animal feed and PMVA completely solves the problem of removing excess moisture from the sapropel immediately after the production and delivery of product to the shore. The process of extraction and preparation for use of sapropel reduced from several days or months (winter freezing) up to several hours.

Single Segment Neo-Bandeau Fronto-Orbital Advancement in Children With Craniosynostosis: Technique Adaptation and Craniometric Analysis.

Fronto-orbital advancement (FOA) of the anterior skull and orbital bandeau is standard of care for craniosynostosis with anterior morphology. Fronto-orbital retrusion, temporal hollowing, and bony contour irregularities are commonly seen in long-term follow-up. In this study, we report several technical adaptations of a new FOA technique described in Fearon et al that help facilitate adaptation of the single-segment neo-bandeau FOA technique in preparation of use in younger patients, and perform a craniometric analysis of the technique. Five consecutive patients who underwent the single-segment neo-bandeau FOA in 2020 with available pre- and post-operative three-dimensional head computed tomography scans were studied. Using Materialise Mimics (Materialise, Ghent, Belgium), cranial length, cranial height, cranial widths, and intracranial volume were measured. Two (40%) patients were male and all were non-Hispanic White with a median age at surgery of 18.6 months (interquartile range 10.4-45.7). Three patients (60%) had bicoronal or other multi-suture craniosynostosis, and 1 each had metopic and sagittal craniosynostosis. Intraoperatively measured intracranial pressure decreased from 17.8 mmHg (R 13.0-20.0) before craniectomy to 4.8 mmHg (R 2.0-11.0; P = 0.038) after craniectomy. Anterior cranial width increased postoperatively (mean 92.6 mm; R 74.9-111.5 versus 117.6 mm; R 109.8-135.2, P = 0.005). Intracranial volume increased from preoperative (mean 1211 cm3; R 782-1949 cm3) to postoperative (1387 cm3; R 1022-2108 cm3; P = 0.009). The authors find in this small sample that a single-segment neo-bandeau FOA demonstrates volumetric expansion similar to conventional FOA techniques and is feasible in infants under 1 year of age.

Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods.

A vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. However, researchers in both basic and clinical settings often face the problem of selecting adequate methods to extract appropriate quality miRNA preparations for use in specific downstream analysis pipelines. This review outlines the variety of different methods of miRNA isolation from biofluids and examines the key determinants of their efficiency, including, but not limited to, the structural properties of miRNA and factors defining their stability in the extracellular environment.