Event Abstract Back to Event Kisspeptin neurons in the mouse hypothalamus: phenotypical characteristics and afferentation to GnRH neurons Barbara Vida1*, Erik Hrabovszky1, Csilla S. Molnár1, Alain Caraty2, Philippe Ciofi3, Clive W. Coen4, Zsolt Liposits1, 5 and Imre Kalló1, 5 1 Hungarian Academy of Sciences, Laboratory of Endocrine Neurobiology, Institute of Experimental Medicine, Hungary 2 INRA//CNRS/Univ Tours/Haras Nationaux, France 3 INSERM U862 Neurocentre Magendie, France 4 King’s College London, School of Biomedical & Health Sciences, United Kingdom 5 Pázmány Péter Catholic University, Department of Neuroscience, Faculty of Information Technology, Hungary In rodents, neurons of the rostral periventricular area of the third ventricle (RP3V) convey the positive feedback action of estrogen onto gonadotropin releasing hormone (GnRH) neurons, whereas neurons in the arcuate nucleus (ARC) have been implicated in the negative feedback effect of this steroid. Neuropeptides released in these areas play significant roles in the regulation of GnRH secretion. Kisspeptin (KP), neurokinin B (NKB) and galanin modulate the episodic release of GnRH, in addition KP and NKB proved to be indispensable in the generation of the preovulatory surge. The aim of our confocal microscopic study was to investigate whether KP neurons of the mouse RP3V and ARC contain galanin and/or NKB. The perikaryal analysis revealed that regardless of the gender the majority of KP neurons in the RP3V are immunoreactive (IR) for galanin. NKB immunoreactive cell bodies were not observed in this brain region. In turn, in the ARC, most of the KP-IR neurons were IR for NKB (females 98%, males 81%, p<0.05). Galanin immunoreactivity was detected in relatively few KP-IR neurons (females 13%, males 5%, p<0.05). The level of colocalisation was determined also in the KP-IR appositions on GnRH neurons. Confocal microscopic analysis of sections from female mice revealed that 30% of KP afferents contained galanin, whereas only 2% of KP-IR varicosities were IR for NKB. These results indicate that either only a small proportion of KP neurons innervate the GnRH neurons from the RPV3 and/or ARC or the amount of co-localizing neuropeptides in the KP-IR afferents of GnRH neurons remained under the detection limit. Further experiments are needed to reveal the background of single-labeled KP appositions and to determine the functional significance of co-existence of galanin and/or NKB with KP in the afferents of GnRH neurons. Supported by OTKA T43407 and T46574, European Union FP6 funding (LSHM-CT-2003-503041), NKFP (1A/002/2004) BBSRC (BBD5231861), Wellcome Trust (060202). Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Vida B, Hrabovszky E, Molnár CS, Caraty A, Ciofi P, Coen CW, Liposits Z and Kalló I (2010). Kisspeptin neurons in the mouse hypothalamus: phenotypical characteristics and afferentation to GnRH neurons. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00139 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Apr 2010; Published Online: 29 Apr 2010. * Correspondence: Barbara Vida, Hungarian Academy of Sciences, Laboratory of Endocrine Neurobiology, Institute of Experimental Medicine, Budapest, Hungary, vidab@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Barbara Vida Erik Hrabovszky Csilla S Molnár Alain Caraty Philippe Ciofi Clive W Coen Zsolt Liposits Imre Kalló Google Barbara Vida Erik Hrabovszky Csilla S Molnár Alain Caraty Philippe Ciofi Clive W Coen Zsolt Liposits Imre Kalló Google Scholar Barbara Vida Erik Hrabovszky Csilla S Molnár Alain Caraty Philippe Ciofi Clive W Coen Zsolt Liposits Imre Kalló PubMed Barbara Vida Erik Hrabovszky Csilla S Molnár Alain Caraty Philippe Ciofi Clive W Coen Zsolt Liposits Imre Kalló Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.