HPV infection is currently one of the most prevalent sexually transmitted disease in the world. In Brazil, according to data from the Ministry of Health, it corresponds to 23.4% of these STD. Considered a pre-neoplastic condition, it has been a focus of attention in health policies because most patients are asymptomatic carriers. HPV detection can be performed by anuscopy associated with anal colposcopy or by evaluating the material obtained from the anal brush by the hybrid capture method. Evaluate the rate of HPV infection with hybrid capture in asymptomatic patients undergoing anuscopy and flexible rectosigmoidoscopy. Prospective study involving 252 patients without related symptons to HPV, with no suspicion at the time of the exam or prior history of HPV. The patients underwent HPV screening using hybrid capture in microplate to HPV from Group A-low-risk (6,11,42,43,44) and high-risk group B (16,18,31,33,3539,45,51,52,58,59,68) with the ability to detect 0.1 virus / cell copy during the examination. Patients with a previous history or diagnosis of HPV were excluded. From the 252 patients evaluated, 136 (53.96%) were male and 116 female, aged 16 to 71 years (mean: 37.4 years). HPV was detected in 50 patients (19.84%): HPV-A in 26 (10.32%) and HPV-B in 24 (9.52%). Of these (48%) presented pathologies at anuscopy, the most frequent being anal microfissures and hemorrhoidal disease. There was no significant difference in HPV prevalence in different age groups. Endoscopic exams may be an opportunity for HPV research in the anal region through this hybrid capture method. In a prospective study, our sample showed a prevalence of asymptomatic HPV infection in the anal region of 19.84%, of which 9.52% presented high-risk strains for cancer (HPV-B).