Premature Graying Of Hair Research Articles

Role of autophagy in the Title aetiopathogenesis of premature graying of hair: a narrative review of literature

Role of autophagy in the Title aetiopathogenesis of premature graying of hair: a narrative review of literature

Shayb (Premature Graying Hair): Risk factors and its Herbal Management - A Systematic Review

Graying of the hair before the age of 20 in Caucasians and before the age of 30 in African Americans is known as Premature Graying Hair (PGH) or Canities. It might have a negative impact on someone's Quality of Life. According to unani system it may be because of weakness of Hazm, poor nutrition of the hair and predominance of Burudat (coldness) in the body. The precise etiopathogenesis is yet unclear. When a patient has PGH, they should be evaluated for metabolic disorders and syndromes. According to Unani System the cause of premature greying of hair is excess production of Balgham (Phlegm) is increased greying of hair. Hair Colors and Colorants can be a transitory treatment choice however aggravation and unfavorably susceptible impacts to certain people are accounted for, it likewise expands the financial burdan. Treatment choices for PGH is calcium pantothenate, PABA, or both in blend and treating the going with obsessive circumstances. Natural Drugs, Nutritional supplements, Unani herbs for both oral and local hair dyes continue to be the primary method of treating cosmetic issues. This review paper explores the detail etiopathogenesis and management of PGH.

Prevalence and Spectrum of Autoimmune Diseases Among Patients with Telomere Biology Disorder

Introduction Telomeres are the protective ends of linear chromosomes that prevent DNA degradation with each replication. Premature attrition or dysfunction of telomeres due to an inherited defect in a telomere maintenance gene results in a spectrum of phenotypes collectively known as telomere biology disorders (TBD). To date, 18 genes have been implicated in TBD. Disease phenotypes are variable including bone marrow failure, immunodeficiency, myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), idiopathic pulmonary fibrosis (IPF), liver cirrhosis, nail dystrophy, and premature graying of hair. Short telomeres have been observed in patients with autoimmune disorders (AID) and have been shown to increase the risk of AID. Furthermore, TBD have been associated with T cell dysfunction and exhaustion, mechanisms which may predispose to autoimmune phenotypes. Given the potential relationship between short telomeres and immune dysregulation, we hypothesize that the prevalence of AID may be increased in patients with a diagnosis of TBD. The aim of this study is to describe the prevalence and spectrum of AID in a cohort of patients with TBD. Methods Health record data were reviewed for 34 adult patients with confirmed TBD treated at the University of Wisconsin-Madison. A TBD diagnosis was established for patients who had a compatible clinical history plus either critically short lymphocyte telomere lengths (<1 st percentile for age as measured by FlowFISH) or one or more pathogenic/likely pathogenic variant(s) in a gene known to cause TBD. Demographics, rheumatologic history, serologies, and family history were collected. AID were included if diagnoses were made by a rheumatologist or other appropriate specialist with clinical features, physical exam findings, and/or serologies meeting diagnostic criteria per subspecialty guidelines. Results Eight of 34 patients (23.5%) with TBD had a concomitant diagnosis of a primary autoimmune condition. Specific AID included inflammatory bowel disease, Graves' disease, Hashimoto's thyroiditis, rheumatoid arthritis, scleroderma, and Sjogren's syndrome (Table 1). Two patients had autoimmune cytopenias, including steroid-responsive immune thrombocytopenic purpura and pure red cell aplasia (PRCA). Patient 8 was diagnosed with PRCA mixed with spur cell anemia prior to transplant, and bone marrow biopsy showed pure erythroid hypoplasia without evidence of malignancy. He responded to treatment with steroids and immunosuppression. Of the eight patients with a primary autoimmune condition, two had pathogenic/like pathogenic variants, two had rare variants of uncertain significance, and four had no detectable variants in a telomere associated gene. Twenty-three patients (67.6%) had a first- or second-degree relative with an autoimmune disorder. Conclusions An AID, as defined by strict diagnostic criteria per subspecialty guidelines, was present in 23.5% of patients with TBD defined by conservative diagnostic criteria. The spectrum of AID was diverse, including two patients with autoimmune hematologic disorders, and the majority had a family history of various AID. Thus, TBD may be associated with an increased prevalence of AID, though confirmation in a larger cohort of genetically defined TBD patients is needed. In the absence of a causative genetic lesion, diagnosis of a TBD based on critically short telomeres in those with multisystem autoimmune disorders with features that overlap TBD phenotypes can be challenging. Screening for AID as part of routine TBD comprehensive assessments as well as offering germline genetic testing for individuals with primary autoimmune disease with a compatible TBD phenotypes and studies to determine the mechanistic relationship between telomere length and autoimmunity are warranted.

Loss of Dicer in Newborn Melanocytes Leads to Premature Hair Graying and Changes in Integrin Expression

Premature hair graying occurs owing to the depletion of melanocyte stem cells in the hair follicle, which can be accelerated by stress caused by genetic or environmental factors. However, the connection between stress and melanocyte stem cell loss is not fully understood. MicroRNAs are molecules that control gene expression by regulating mRNA stability and translation and are produced by the enzyme Dicer, which is repressed under stress. In this study, using 2 mouse genetic models and human and mouse cell lines, we found that the inactivation of Dicer in melanocytes leads to misplacement of these cells within the hair follicle, resulting in a lack of melanin transfer to keratinocytes in the growing hair and the exhaustion of the melanocyte stem cell pool. We also show that miR-92b, which regulates ItgaV mRNA and protein levels, plays a role in altering melanocyte migration. Overall, our findings suggest that the Dicer-miR92b-ItgaV pathway serves as a major signaling pathway linking stress to premature hair greying.

Identification of Critical Biomarkers and Mechanisms of Fructus Ligustri Lucidi on Vitiligo Using Integrated Bioinformatics Analysis.

Vitiligo is an autoimmune disease of the skin that targets pigment-producing melanocytes and results in patches of depigmentation that are visible as white spots. Recent research studies have yielded a strong mechanistic understanding of this disease. Fructus Ligustri Lucidi (FLL) has been used for premature graying of hair since ancient China and is currently used to treat vitiligo. However, the key biomarkers and mechanisms underlying FLL in vitiligo remain unclear. This study aimed to identify the potential biomarkers and mechanisms of FLL in vitiligo using network pharmacology analysis. The expression profiles of GSE65127 and GSE75819 were downloaded from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) between the vitiligo and healthy samples. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs were performed using R analyses. We performed R to further understand the functions of the critical targets. Cytoscape tools have facilitated network topology analysis. Molecular docking was performed using Auto Dock Vina software. The results showed that 13 DEGs were screened in vitiligo. Based on bioinformatics, network pharmacology and Western blot, we found that the critical targets of melanoma antigen recognized by 5,6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1) may be related to the mechanism of action of FLL in the treatment of vitiligo. TYRP1, as a melanocyte molecular biomarker, may be closely related to the underlying mechanism of FLL in the treatment of vitiligo via the inhibition of melanocyte death.

EFFICACY OF MADHUKA TAILA NASYA AND JAPAAKUSUMA LEPA IN AKAALAPALITYA-A SINGLE CASE STUDY

Hair is an aesthetic tool. The visible sign of ageing is hair graying. Premature graying of hair is a burning problem faced by youth today. According to WHO, the incidence is high in the age group of 20-30 years. The depletion of melanocytes leads to premature hair graying. In Ayurveda, it is termed Akaalapalitya and is mainly due to the vitiation of Vata and Pitta. Contemporary medical science does not offer satisfactory treatment. Hair dyes cause damage to hair due to harsh chemicals. Hence there is a need for Ayurvedic intervention for finding safe and effective remedies for the management of Akaalapalitya. Nasya is one of Panchakarma and is indicated in the treatment of Urdhwajatrugata vyadhi. Lepa application externally on hair has been mentioned in our classics for the treatment of Akaalapalitya. The holistic approach of Ayurveda is the need of the hour in the treatment of Akaalapalitya without any adverse effects. In this single case study patient fulfilling inclusion criteria and diagnosed with Akaalapalitya has been treated and benefitted by Madhuka taila nasya and Japaakusumadi lepa.

A review of Kesha as diagnostic and prognostic tool in Ayurveda

Hair is one of the essential characteristic features which not only adds beauty to the person but also helps maintain normal physiological functions, especially by excreting sweat through the Lomakupa, which is considered one of the roots of Swedavaha Strotas. It also helps in thermoregulation, protection etc. It is Pitruja Bhava and is the Mala of Asthidhatu. Depending upon the Hair conditions, one can assess some of the underlying pathological status of the body. Aims & Objectives: Review the literature concerned with Prakruta and Vikruta Lakshnas of hairs in Ayurvedic classical texts for diagnosis and prognosis of the disease. Material and Methods: Material related to Hair has been collected from Charaka Samhita, Sushruta Samhita, Astanga Samgraha, and Ashtanga Hridaya along with respective commentaries available and data associated with a topic general at different journals and books. Observations: As Kesha is one of the factors which build up a person's personality, Ayurvedic classics state the advantages of hair maintenance. In the present era, changes in lifestyle, food habits, excess use of shampoos, and hair dyes, lack of oil massage to Hair, pollution, stress and strain etc., may be the cause of different types of hair problems like Khalitya (Alopecia totalis), Palitya (premature greying of Hair) etc. Therefore a proper understanding of Prakruta Lakshanas of Kesha will help in understanding the Vaikrutha Avastha which will thus ease the line of treatment. Results: Thus, Kesha and Loma play an essential role in diagnosing the Prakruta and Vaikrutha Lakshana and help in the prognosis of the disease.

A REVIEW ON PALITYA AND DHATRYADI RASAYANA

Hair is a valuable anatomical structure of the body, having cosmetological and sociological importance in drafting the beauty and personality of an individual. Greying of hair represents the sign of ageing known as Palitya in Ayurveda. But when hair starts greying before the usual age of onset, known as premature greying of hair. Premature hair greying is called Akalaja Palitya, which occurs due to various factors resulting in the vitiation of Pitta dosha. Due to mankind’s hectic, sedentary and busy schedule, the current scenario is witnessing several challenges, and premature greying of hair is one among them. Premature hair greying has multifactorial aetiology, including oxidative stress, nutritional deficiency, smoking, and dysfunctional thyroid hormones, but the exact aetiology is still unknown. Acharyas have mentioned various rasayana for preventing and treating premature hair greying, i.e., Akalaja Palitya. Rasayana therapy aims primarily to promote the strength and vitality of the body. It also helps maintain the integrity of sapta dhatus (fundamental structural component) by improving digestive power resulting in the sound production of successive dhatus and their malas. Dhatryadi rasayana is a polyherbal formulation consisting of three ingredients, i.e., Dhatri (Emblica officinalis Gaertn.), Bhringraj (Eclipta alba Hassk.) and Tila (Sesamum indicum Linn.). All three plants have the keshya property. Emblica officinalis Gaertn. and Sesamum indicum Linn. both act as a 5α-reductase inhibitor. Eclipta alba Hassk. stimulates the proliferation of follicular keratinocytes by downregulating the TGF-β1 (Transforming Growth Factor Beta 1) expression. Furthermore, all ingredients of Dhatryadi rasayana have antioxidant properties and contribute to essential nutritional supplements required for proper hair development and growth.

BMI1 is required for melanocyte stem cell maintenance and hair pigmentation.

The epigenetic repressor BMI1 plays an integral role in promoting the self-renewal and proliferation of many adult stem cell populations, and also tumor types, primarily through silencing the Cdkn2a locus, which encodes the tumor suppressors p16Ink4a and p19Arf . However, in cutaneous melanoma, BMI1 drives epithelial-mesenchymal transition programs, and thus metastasis, while having little impact on proliferation or primary tumor growth. This raised questions about the requirement and role for BMI1 in melanocyte stem cell (McSC) biology. Here, we demonstrate that murine melanocyte-specific Bmi1 deletion causes premature hair greying and gradual loss of melanocyte lineage cells. Depilation enhances this hair greying defect, accelerating depletion of McSCs in early hair cycles, suggesting that BMI1 acts to protect McSCs against stress. RNA-seq of McSCs, harvested before onset of detectable phenotypic defects, revealed that Bmi1 deletion derepresses p16Ink4a and p19Arf , as observed in many other stem cell contexts. Additionally, BMI1 loss downregulated the glutathione S-transferase enzymes, Gsta1 and Gsta2, which can suppress oxidative stress. Accordingly, treatment with the antioxidant N-acetyl cysteine (NAC) partially rescued melanocyte expansion. Together, our data establish a critical function for BMI1 in McSC maintenance that reflects a partial role for suppression of oxidative stress, and likely transcriptional repression of Cdkn2a.

Early Canities among Undergraduate Medical Students of a Medical College: A Descriptive Cross-sectional Study

Early canities are the premature greying of hair before the age of 25 years in Asians. The condition is a matter of concern for young adults aesthetically. This study aimed to find out the prevalence of early canities among undergraduate medical students of a medical college. A descriptive cross-sectional study was carried out from 1 December 2021 to 30 June 2022 among undergraduate medical students at a medical college. The study was conducted after receiving ethical approval from the Institutional Review Committee [Reference number: 146(6-11)C-2 078/079]. The participants with ages less than 25 years without a history of vitiligo, intake of chemotherapeutic drugs, progeria, pangeria and recent dyeing of hair were enrolled. A convenience sampling method was used. Point estimate and 95% Confidence Interval were calculated. Out of 235 students, 95 (40.42%) (34.15-46.69, 95% Confidence Interval) had early canities. The most prevalent premature greying was grade I early canities i.e.79 (83.15%) of participants. Among the participants with early canities, 56 (58.94%) were male, 41 (43.15%) had a positive family history for early canities, 67 (70.52%) had normal body mass index and 38 (40%) had O+ve blood group. The prevalence of early canities among undergraduate medical students was lower than in other studies done in similar settings. The grade I early canities was seen more among the participants with premature greying of hair. epidemiology; hair colour; medical students; physiology.

Evaluation of Serum Paraoxonase and Prolidase Levels in Patients with Premature Graying Of Hair

Evaluation of Serum Paraoxonase and Prolidase Levels in Patients with Premature Graying Of Hair

Tell me more – telomere biology and understanding telomere defects

Telomeres are DNA-protein structures at the ends of chromosomes that maintain chromosome stability and protects them from damage during mitosis and prevents abnormal fusion with adjacent chromosomes. They are non-coding repeats of the (TTAGGG)n nucleotide sequence. Telomere length affects cell replication potential and cell senescence. There are numerous proteins and enzymes that protect and maintain telomere length. Telomere disorders are a heterogenous group of diseases caused by germline pathogenic variants in genes involved in telomere maintenance. The clinical features are variable but often include bone marrow failure, idiopathic pulmonary fibrosis (IPF), liver cirrhosis, premature greying of hair, and dysplastic nails. However, incomplete penetrance and variable expressivity influence the clinical phenotype in individuals, which results is underdiagnosis or late diagnosis for many patients. At birth, telomere length on average in white blood cells (WBCs) is estimated to be 10Kb and the rate of attrition ranges from 27–41 base pairs each year. There are many factors that affect telomere length but genetics plays a significant role. Also, telomere length can vary in different cells in the same individual based on variable local gene expression. Idiopathic pulmonary fibrosis (IPF) is the most common telomere defect and approximately 30% of patients with sporadic or familial IPF have genetic defects in telomere-related genes. This talk will cover fundamental concepts of telomeres and discuss short and long telomere defects and measurement of telomere length in the clinical laboratory.

A brief overview on the concept of Palityaw.s.r. premature hair greying

Introduction: The traditional Indian medical system of Ayurveda employs the term Khalitya (~Hair fall) to describe hair loss, while the premature greying of hair is denoted by the term Palitya (~ Hair greying). Khalitya and Palitya are classified as Paitik (~dosha regulating body temperature and metabolic activities) disorders, which originate from the Dosha (~the regulatory functional factors of the body formed by the synchronized interplay between the five basic elements within any living system) known as Pitta. According to Ayurvedic principles, consistent disruption of the Pittadosha (~dosha regulating body temperature and metabolic activities), which represents the body’s heat and metabolism, may result in premature greying of hair. As per Ayurveda principles, the consumption of substances that enhance Pitta can lead to an aggravation of Pittadosha (~dosha regulating body temperature and metabolic activities), which is believed to be a contributing factor in the manifestation of grey hair. Materials and methods: Palitya (~premature greying of hair) information is gathered from many Ayurvedic and modern medical textbooks, magazines, journals, and the internet. It is a review paper with data gathered from all relevant sources. Result: All of the aforementioned formulations were acquired as a result of premature greying of hair. Discussion & Conclusion: Ayurveda has been identified as a viable alternative therapeutic approach for the condition of premature greying that was natural and harmless.

Prevalence of Premature Hair Greying, its Associated Factors and Impact on Quality of Life in Medical Students from Rajasthan, India

Background: Considering the paramount significance of hair in life, greying hair at a young age can be extremely distressing. In addition, an incompletely understood aetiology and scarcity of treatment options make premature hair greying (PHG) noteworthy. Aims: We aimed to estimate the prevalence of PHG in medical college students from Rajasthan, India, and any sociodemographic and lifestyle correlates of PHG and to determine its effect on the quality of life (QOL). Patients and Methods: A cross-sectional study was conducted amongst 295 students of a medical college in western Rajasthan, India, under the age of 25 years. A scalp examination was done to count grey hair. All factors were investigated using structured, pre-validated questionnaires. Results: The prevalence of premature greying of hair was 41.4%. Sociodemographic factors such as older age, rural residence and positive family history were significantly associated with PHG. The number of meals, fruit consumption and irregularity of meals were the lifestyle factors associated with PHG. The QoL of 54.1% of students with grey hair was poor; males and rural residents were more affected. Conclusions: Premature greying of hair is a fairly prevalent condition in medical college students affecting their QoL. Factors such as family history, age, residence and eating habits may predispose students to PHG.

Generation of Pmel-dependent conditional and inducible Cre-driver mouse line for melanocytic-targeted gene manipulation.

Conditional and inducible gene targeting using Cre/loxP-mediated recombination is a powerful reverse genetics approach used to study spatiotemporal gene functions in specified cell types. To enable temporal gene manipulation in the melanocyte lineage, we established a novel inducible Cre-driver mouse line by targeting an all-in-one tetracycline/doxycycline (Dox)-inducible Cre expression cassette into the Pmel locus (PmelP2A-TetON3G-TRE3G-iCre ), a gene locus preferentially expressed in pigment cells. By crossing these Cre-driver mice with a strong Cre-reporter mouse line, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze , we show the effectiveness of the PmelP2A-TetON3G-TRE3G-iCre mouse line in facilitating Dox-inducible Cre/loxP recombination in a wide variety of pigment cell lineages including hair follicle melanocytes and their stem cells. Furthermore, to demonstrate proof of concept, we ablated Notch signaling postnatally in the PmelP2A-TetON3G-TRE3G-iCre mice. In agreement with the previously reported phenotype, induced ablation of Notch signaling in the melanocyte lineage resulted in premature hair graying, demonstrating the utility of the PmelP2A-TetON3G-TRE3G-iCre allele. Therefore, the PmelP2A-TetON3G-TRE3G-iCre mouse line is suitable for assessing gene functions in melanocytes using an in vivo inducible reverse genetics approach. Furthermore, we unexpectedly identified previously unrecognized PMEL-expressing cells in non-pigmentary organs in the mice, suggesting unanticipated functions of PMEL other than melanosome formation.

Palitya w.s.r to Premature Graying of Hair -An Ayurvedic Overview

Premature graying of hair is referred to as "Palitya" in the Ayurvedic method and falls under the general category of "Shiroroga."Palitya mainly shows Dosha’s imbalance of our body. This premature graying of hair refers to the graying of hair in young age or before the age of 35 years. In Ayurveda this is known as “Palitya”. Grey Hair is caused by gradual reduction of melanin production overtime in your hair follicles. Melanin a pigment that gives colour and smoothness to your hair. Eventually the hair begins to lose its colour. Ayurveda says, the “Bhrajaka Pitta a pigment responsible for imparting natural colour to our skin as well as to hair also seated in superficial layer of skin (Avabhasini). Soft, lustrous, silky, black hair are the sign of long healthy life span as per said by Acharya Charaka. In the present era sedentary life style, imbalanced diet, tense, mental conditions and polluted hair, water and food have led to lot of complications which are difficult to remove. Therefore global interest these days is developing towards Ayurveda to face the Challenge be it preventive aspect or curative treatment method of pacification, management of disease in its initial stage or final stage. To gather all information related to Palitya at one place which is availed in different Ayurvedic texts and for elucidation of this knowledge in a proper manner the present study has been undertaken.

A Pilot Study on Evaluation of Formulated Herbal Hair Oil

Hair follicles are surrounded by the dermis, but the cells are part of the epidermis and are separated from the dermis by the basal lamina layer. The main aim of the study is to design and evaluation of versatile Herbal Hair Oil to prevent and control hair-related problems. The main objectives of the study are Provides hair conditioning, Prevention & control of hair fall, Reduce scalp irritation, Prevents premature greying of hair, Treats Dandruff, Relieves all types of headaches especially migraine & Hangover headaches gives a shiny and glossy appearance.

Ethnopharmacological study about Glycyrrhiza glabra L. (Licorice) based on Ayurveda, An Indian System of Traditional Medicine- A Review

Glycyrrhiza glabra L. (GG), also known as Yashtimadhu, is a traditional plant which is widely used in Ayurveda. It is used to treat a variety of ailments including cough, asthma, bronchitis, diabetes, gastric ulcers, skin problems, inflammation, anaemia, premature greying of hair and others. Clinical and experimental research suggests that it possesses anti-inflammatory, antiviral, immunomodulatory, hepatoprotective, antimicrobial, antioxidant, anticancer, and cardioprotective activities. We gathered material on the phytochemistry, pharmacology, and toxicity of Glycyrrhiza glabra published before March 2022 from PubMed, Springer, SCOPUS, Google Scholar and Science Direct. To compile this study, we conducted a thorough analysis of the classical Ayurvedic treatises in order to obtain a full account of the qualities, actions, and applications stated in Ayurveda about yashtimadhu in order to demonstrate ancient-modern concordance. GG has been studied for a variety of pharmacological properties, including ulcer healing, anti-ulcerogenic, anti-bacterial, antioxidant potential activity, anti-asthmatic activity, hair growth stimulating activity, and memory enhancing action. Licorice in Ayurveda is generally used in Ayurveda as a rejuvenator and aphrodisiac, as well as for boosting memory, complexion, sound quality, dental health, vision, lactation, and healing cancer, ulcers and wounds. Even though GG is a widely used Ayurveda medicine, there is a lack of evidence regarding the true medicinal potential of the drug. There are innumerable actions mentioned in the Ayurveda classical textbooks. Here, an effort is made to find substantiation for the ancient wisdom through a thorough search with the modern available literature.

A Study of Micronutrient Levels in Premature Canities in Children

Abstract Background: Premature canities refer to the loss of hair pigment, at an earlier age than normal. Vitamins and minerals are involved in the various steps of melanogenesis. There is a paucity in the current literature on possible correlation between the serum levels of micronutrients and premature graying of hair. Materials and Methods: A case − control study was conducted on 56 patients of premature canities and 50 age- and sex-matched healthy controls between 12 and 18 years of age. After taking detailed history and physical examination, serum was analyzed for copper, zinc, iron, magnesium, Vitamin B12, folic acid, and Vitamin D levels in both cases and controls, and the results were compared. Results: Mean serum levels of zinc, magnesium, and Vitamin B12 were significantly lower in cases (P < 0.01). No significant difference between mean serum copper, iron, Vitamin D, and folic acid levels between cases and controls was found. Patients having deficiency of copper, zinc, iron, magnesium, and Vitamin B12 were significantly higher in cases compared to controls (P < 0.05). No significant association between the serum levels of micronutrients with severity of canities was found. Conclusion: On basis of our study, we conclude that patients of premature graying can have micronutrient deficiencies such as copper, zinc, iron, magnesium, and Vitamin B12. These micronutrients should be analyzed in patients of premature canities if facilities are available and if patients are found deficient, they should be supplemented for the same. However, further studies on a larger population and effect of supplementation of these micronutrients on premature graying are required.

In Vitro hair growth and hair tanning activities of mangosteen pericarp extract on hair dermal papilla cells

Premature hair graying and hair loss in men and women can cause a serious aesthetic problem. However, not much effective treatment is available, especially from natural sources. In this study, the efficacy of mangosteen pericarp water extract in promoting hair growth and tanning activities on hair dermal papilla cells was studied by using in vitro assays. The proliferation and cytotoxicity study on hair dermal papilla cells treated with mangosteen pericarp extract were measured by 3-(4,5-dimethylthiazol-2- yl)− 2,5-diphenyltetrazolium bromide (MTT) and Sulforhodamine B assay. The degree of hair dermal papilla cells proliferation increased with increasing concentration of the extract up to 500 μg/ml of extract treatment. The cell proliferation significantly increased by 157.56 % compared to the control with 500 μg/ml of extract treatment. The mangosteen pericarp extract was found to stimulate melanin synthesis and tyrosinase activity of hair dermal papilla cells in a concentration-dependent manner. The melanin synthesis in hair dermal papilla cells was upregulated more than four-fold with mangosteen pericarp extract as compared to the control group. The mangosteen pericarp extract was more potent and better in comparison with known effective melanogenic agents such as α-melanocyte stimulating hormone and forskolin in inducing the melanogenic effects of hair dermal papilla cells.