The effects of dobutamine (DOB) on myocardial performance, systemic hemodynamics, and oxygen delivery during acute normovolemic hemodilution in anesthetized rats were studied. Forty-two Sprague Dawley rats (body weight 375 to 425 g) were divided into six equal groups. Hemodynamic and cardiac indices were measured or calculated at baseline, 30 minutes after the initiation of hemodilution (HD), and 15 minutes after DOB or saline infusion. Myocardial performance in response to acute pressure or volume loads was studied in all groups of animals. HD to a hematocrit (Hct) value of 20% resulted in no change in heart rate (HR), increased CI, SVI, and LV dP/dt, and decreased MAP, SVRI, and oxygen delivery index (O 2DI). HD increased peak SV and Cl after preload stress while the left ventricular developed pressure (LVDP) was unchanged. Infusion of DOB as 7.5 or 15 μg/kg/min increased HR, CI, and LV dP/dt as well as LVDP. At the same time, DOB decreased MAP and SVR, whereas the SVI remained unchanged. In non-HD animals both doses of DOB increased LVDP, but only the larger dose increased CI, whereas peak SV decreased with the smaller dose. Arterial carbon dioxide tension (PaCO 2) increased, whereas pH and arterial oxygen tension (PaO 2) decreased; however, O 2DI remained unchanged. Concomitant hemodilution and DOB infusion resulted in attenuation of HR response to DOB, exaggerated the drop in MAP and SVR, and increased LV dP/ dt. Only the larger dose of DOB increased the CI, whereas neither dose could alter the SVI in HD animals. Whereas HD increased the peak CI and SVI, administration of DOB to HD animals did not change LVDP, peak Cl, or peak SVI. Administration of DOB to HD animals decreased pH and increased PaCO 2)but O 2DI was not changed. The study demonstrated that the use of DOB to increase the physiologic compensatory mechanisms induced by HD was not effective, and the hemodynamic profile of DOB obtained in HD rats was significantly different from that observed in the non-HD groups. Therefore, it could be suggested that in normovolemic HD, DOB may fail to increase myocardial performance against sudden increase in afterload or preload although DOB can increase the CI in the resting or unstressed state.
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