Preliminary Evaluation Research Articles

Usability of fractometer for the purpose of a practical preliminary assessment tool for wood density of Pinus brutia

The fractometer is a device that breaks increment cores to measure fracture strength. The advantages of the device are that it is relatively fast, easy to use in the field, and it can perform direct strength measurements on increment cores. The main purpose of the study was to evaluate the usability of the fractometer as a preliminary evaluation tool for wood density traits of standing Turkish Pinus brutia (red pine) trees. Fracture strength was measured on 5 mm diameter increment cores, and X-ray densitometry was used for density measurements. Due to the high correlation between the two traits (fracture and density), a model was built using linear regression. Fifty trees were sampled to build the statistical model (r2: 0,74), and an equal sample size was used to test the model. The density value obtained from the model was 0,546 gcm-3, while the density value averaged by the X-ray method for the same group was 0,543 gcm-3. When considering mean values, it can be said that the model provides a good prediction. Based on personal experience and research results, some trees exhibited better growth and wood quality traits than others. Breeding from these trees could offer improvement in timber production and performance for Pinus brutia (red pine). As a general consequence, the fractometer and increment core sampling can be used for pine tree breeding programs for the preliminary assessment of wood density.

Global 500 m seamless dataset (2000–2022) of land surface reflectance generated from MODIS products

Abstract. The Moderate Resolution Imaging Spectroradiometer (MODIS) is widely utilized for retrieving land surface reflectance to reflect plant conditions, detect ecosystem phenology, monitor forest fires, and constrain terrestrial energy budgets. However, the state-of-the-art MODIS surface reflectance products suffer from temporal and spatial gaps due to atmospheric conditions (e.g. clouds and aerosols), limiting their use in ecological, agricultural, and environmental studies. Therefore, there is a need for reconstructing spatiotemporally seamless (i.e. gap-filled) surface reflectance data from MODIS products, which is difficult due to the intrinsic inconsistency of observations resulting from various sun/view geometry and the prolonged missing values resulting from polar night or heavy cloud coverage, especially in monsoon season. We built a framework for generating the global 500 m daily seamless data cubes (SDC500) based on MODIS surface reflectance dataset, which contains the generation of a land-cover-based a priori database, bidirectional reflectance distribution function (BRDF) correction, outlier detection, gap filling, and smoothing. The first global spatiotemporally seamless land surface reflectance at 500 m resolution was produced, covering the period from 2000 to 2022. Preliminary evaluation of the dataset at 12 sites worldwide with different land cover demonstrated its robust performance. The quantitative assessment shows that the SDC500 gap-filling results have a root-mean-square error (RMSE) of 0.0496 and a mean absolute error (MAE) of 0.0430. The SDC500 BRDF correction results showed an RMSE of 0.056 and a bias of −0.0085 when compared with MODIS nadir BRDF-adjusted reflectance (NBAR) products, indicating the acceptable accuracy of both products. From a temporal perspective, the SDC500 eliminates abnormal fluctuations while retaining the useful localized feature of rapid disturbances. From a spatial perspective, the SDC500 shows satisfactory spatial continuity. In conclusion, the SDC500 is a well-processed global daily surface reflectance product, which can serve as the fundamental input for large-scale ecological, agricultural, and environmental applications and quantitative remote sensing studies. The SDC500 is available at http://data.starcloud.pcl.ac.cn/resource/27 (Liang et al., 2023b) or https://doi.org/10.12436/SDC500.27.20230701 (Liang et al., 2023a).

Potential oral VEGFR2 inhibitors: Treatment of wet age-related macular degeneration

Wet age-related macular degeneration (w-AMD) is one of the leading causes of vision loss in industrialized countries. A large body of evidence suggests that inhibitors targeting VEGFR2 may be effective in the treatment of w-AMD. The identification of an oral VEGFR2 inhibitor for the treatment of w-AMD provides an opportunity for a route of administration other than intravitreal injection. While screening potent VEGFR2 inhibitors at the enzyme and cellular levels, ensuring the safety of the compounds was our primary strategy for screening optimal compounds. Finally, compound 16 was identified, exhibiting enhanced inhibition of VEGFR2 enzyme and proliferation of BaF3-TEL-VEGFR2 cells compared to Vorolanib. Compound 16 had a weak inhibitory effect on human Ether-a-go-go-related gene (hERG) channel currents, showing a cardiac safety profile similar to Vorolanib. Compound 16 showed no significant toxicity to human liver cell LX-2, indicating a liver safety profile similar to Vorolanib. The water solubility of compound 16 was found to be higher than that of Vorolanib when tested at pH = 7.4. In addition, compound 16 was found to inhibit VEGFR2 phosphorylation in human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner by WB assay. Furthermore, the in vitro preliminary evaluation of the drug-like properties of compound 16 showed remarkable plasma stability and moderate liver microsomal stability. Based on in vivo pharmacokinetic studies in ICR mice, compound 16 exhibited acceptable oral bioavailability (F = 20.2 %). Overall, these findings provide evidence that compound 16 is a leading potential oral drug candidate for w-AMD.

Preliminary evaluation of NEG materials in view of CMSB replacement in HCPB TER architecture

CMSB (Cryogenic Molecular Sieve Beds) operated at 77 K is used for tritium recovery in the reference design of HCPB (Helium Cooled Pebble Bed) TER (Tritium Extraction and Removal) system. However, this technology has two main drawbacks: large consumption of liquid nitrogen and the controlling of tritium stream sent to the Tritium Plant, during the regeneration of the CMSB, in such way to avoid large variations in the gas flow rate.This paper reports on a preliminary evaluation for CMSB replacement by non-evaporable getter materials for tritium recovery. The advantages of NEG technology are the high specific pumping speed and capacity and the ability to withstand a large number of absorption/desorption cycles. In addition, NEG do not release hydrogen unless power is supplied to heat them, addressing an important safety issue related to uncontrolled release in case of power outage or subsystem failures.

Qualitative Evaluation of a Pilot Physician Training Program in Long-Term Care Facilities in Switzerland

Increasing demand for long-term care facilities (LTCFs) and increasingly complex medical needs of LTCF residents necessitate recruiting new physician graduates and developing programs which provide them with experience and specific skills in geriatric and palliative medicine. We developed an educational program for physicians in training, which combines 1 year of immersion at 20% full-time equivalent with (1) theoretical teaching, (2) clinical exposure under the supervision of a senior LTCF physician, and (3) structured case presentations and debriefing with experienced geriatricians. This article presents a preliminary qualitative evaluation of this program from the perspectives of the trainees, supervising physicians, and public health stakeholders. The program was well accepted by all parties and showed positive effects on trainees’ interest in long-term care medicine. Suggestions for improving future programs and their implementation are provided.

Faster Refill in an Urban EMS System Saves Lives: A Prospective Preliminary Evaluation of a Prehospital Advanced Resuscitative Care Bundle.

Military experience has demonstrated mortality improvement when advanced resuscitative care (ARC) is provided for trauma patients with severe hemorrhage. The benefits of ARC for trauma in civilian EMS systems with short transport intervals are still unknown. We hypothesized that ARC implementation in an urban EMS system would reduce in-hospital mortality. This was a prospective analysis of ARC bundle administration between 2021 and 2023 in an urban EMS system with 70,000 annual responses. The ARC bundle consisted of calcium, tranexamic acid (TXA), and PRBCs via a rapid infuser. ARC patients were compared to trauma registry controls from 2016 to 2019. Included were patients with a penetrating injury and SBP < 90 mmHg. Excluded were isolated head trauma or prehospital cardiac arrest. In-hospital mortality was the primary outcome of interest. A total of 210 patients (ARC = 61, controls = 149) met criteria. Median age was 32 years, with no difference in demographics, initial SBP or heart rate recorded by EMS, or new injury severity score (NISS) between groups. At hospital arrival, ARC patients had lower median heart rate and shock index than controls (p < 0.03). Fewer patients in the ARC group required prehospital advanced airway placement (p < 0.001). 24-hour and total in-hospital mortality were lower in the ARC group (p < 0.04). Multivariable regression revealed an independent reduction in in-hospital mortality with ARC (OR 0.19, 95%CI 0.05-0.68, p = 0.01). Early ARC in a fast-paced urban EMS system is achievable and may improve physiologic derangements while decreasing patient mortality. ARC closer to the point of injury warrants consideration. Level IV, Prospective.

Exploring the impact of a co-designed shared book reading environment for families in a community hub.

Evidence supports the effectiveness of shared book reading for promoting language and literacy development, but it is known that families experiencing vulnerability may have reduced access to books and are less likely to share books regularly at home. Community hubs often provide support to families experiencing vulnerability and may provide an opportunity to create environments that support families to engage in shared book reading, especially if families are invited into the creation of these environments through co-design. However, there is currently little evidence regarding the impact of co-designing shared book reading environments with families in community settings. The current study is part of a broader project which used co-design to develop a shared book reading environment in collaboration with stakeholders in a community hub. This small-scale study aimed to provide a preliminary evaluation of the impact of this co-designed shared book reading environment at the community hub on (1) the frequency of shared book reading at the hub; (2) participants' confidence and enjoyment of reading with children at the hub; and (3) participants' experiences of shared book reading at the hub. The co-design project was carried out in four phases with families, staff and community partners at the community hub. A convergent mixed-methods approach was used to collect and analyse quantitative and qualitative data in the first and last phase of the project to evaluate project outcomes. Quantitative data (environmental observations of reading at the hub and participant ratings of confidence and enjoyment of reading with children at the hub) were analysed using descriptive statistics and nonparametric statistical tests. Qualitative data (participants' responses regarding their experiences of changes implemented in the community hub) were analysed using inductive content analysis. An increased frequency of shared book reading was observed within the community hub after changes were implemented, and positive changes in enjoyment of book reading were recorded. Qualitative responses from participants following conclusion of the project were organised into three main categories, which showed that (1) children and families were reading more and enjoying reading activities that were happening; (2) children and families were borrowing more books; and (3) families had changed the way they were reading at home with their children. A co-designed shared book reading environment within a community hub had a positive impact on shared book reading experiences for families in areas with high levels of vulnerability. What is already known on this subject Community hubs provide a unique opportunity to support early language and literacy development in partnership with families in areas of vulnerability; however, there is limited literature investigating the impacts of these supports. A previous study utilised co-design to develop a shared book reading environment with stakeholders at a community hub in an area where a high proportion of children are considered to be 'vulnerable' in terms of language and literacy development. It was found that co-design enabled the development of collaborative changes to support shared book reading that were valued and owned by families, staff and community partners at the community hub. What this study adds The current study focused on evaluating the impact of a co-design project on the frequency of shared book reading at the hub and participants' experiences, confidence and enjoyment of reading with children at the hub. Analysis of pre- and post-data provide preliminary evidence that a co-designed shared book reading environment in a community hub can have a positive impact on increasing families' awareness and engagement with book borrowing, engagement in shared book reading activities in the community and at home, and participants' confidence and enjoyment of reading with children. What are the clinical implications of this work? Speech pathologists have an important role to play in prevention and promotion. This study has shown that collaboration with families experiencing vulnerability can support the implementation of strategies to promote shared reading in a community hub and demonstrate increased quality and frequency of shared book reading. The utilisation of a community hub also highlights the opportunities for the speech pathology profession to deliver community-based promotion and prevention initiatives as a strategy to address equitable language outcomes.

Using human-centered design to develop and implement a pediatric mental health care access program.

In 2019, the University of California at San Francisco (UCSF) launched the Child and Adolescent Psychiatry Portal (CAPP), a pediatric mental health care access (PMHCA) program providing remote mental health consultation services to pediatric primary care providers (PCPs) throughout Northern and Central California. The development and implementation of CAPP was guided by Human-Centered Design (HCD), an iterative, rapid-paced innovation process focusing on stakeholders' needs and experiences, which shaped the development of CAPP's programs. The resulting key programmatic elements are designed for pediatric workforce development: (1) PCP consultation with a child and adolescent psychiatrist via a telephone warmline; and (2) training and education for providers. CAPP has grown rapidly since its launch, having enrolled 1,714 providers from 257 practices spread across 36 counties and provided 3,288 consults on 2,703 unique lives as of August 2023. Preliminary evaluation findings indicate high PCP satisfaction with CAPP's services, despite continued challenges of integrating behavioral health into primary care. Throughout the HCD and implementation process, multidisciplinary partnerships have proven critical in providing end-user input to inform and improve program design. This growing network of partnerships, developed through the cultivation of personal relationships and trust over time, has also proven essential for CAPP's rapid growth and sustainability. Overall, this Community Case Study highlights the critical role of partnerships and the importance of taking a people-centered approach, as captured in CAPP's motto, "Connecting for Care."

Preliminary Evaluation of Methods for Continuous Carbon Removal from a Molten Catalyst Bubbling Methane Pyrolysis Reactor

Methane pyrolysis in molten catalyst bubble (MCB) column reactors is an emerging technology that enables the simultaneous production of hydrogen and solid carbon, together with a mechanism for separating the two coproducts. In this process, methane is dispersed as bubbles into a high temperature molten catalyst bath producing hydrogen and low-density carbon, which floats to the surface of the bath from providing a means for them to be separated. However, the removal of carbon particulates from a bubbling column reactor is technically challenging due to the corrosive nature of the molten catalysts, contamination of the product carbon with the molten catalysts, high temperatures and lack of understanding of the technology options. Four potential concepts for the removal of carbon particulate from a methane pyrolysis molten metal bubble column reactor are presented, based on the pneumatic removal of the particles or their overflow from the reactor. The concepts are evaluated using a cold prototype reactor model. To simulate the operation of a high-temperature reactor at low temperatures, the dominant dimensionless numbers are identified and matched between a reference high-temperature reactor and the developed cold prototype using water, air and hollow glass microsphere particles as the representatives of the molten catalyst, gaseous phases and solid carbon particulates, respectively. The concepts are tested in the cold prototype. High rates of particle removal are achieved, but with different tradeoffs. The applicability of each method together with their advantages and disadvantages are discussed.

Educational effects of and satisfaction with mixed-reality-based major trauma care simulator: A preliminary evaluation.

Mixed reality (MR) is a hybrid system that projects virtual elements into reality. MR technology provides immersive learning using various real-world tools. However, studies on educational programs using MR are scarce. This study aimed to investigate the educational effects of and satisfaction with an MR-based trauma decision-making simulator. A total 40 of trainees self-selected to participate in this study. All of them participated in the MR trauma simulator for approximately 30 minutes and conducted voluntary learning without any external help. Declarative knowledge, measured using 20 multiple-choice questions, was assessed before and after MR trauma training. To confirm the educational effect, test scores before and after MR trauma training were compared using a paired t-test. Student satisfaction after training was measured using a ten-item questionnaire rated on a five-point Likert scale. A pretest-posttest comparison yielded a significant increase in declarative knowledge. The percentage of correct answers to multiple choice questions increased (from a mean of 42.3, SD 12.4-54.8, SD 13) after the MR-based trauma assessment and treatment training (P < .001). Of the participants, 79.45% were satisfied with the overall experience of using the MR simulator. This study demonstrated a meaningful educational effect of the MR-based trauma training system even after a short training time.

Formulation, Development, and Characterization of Carboxymethyl Chitosan Triggered Calcium Carbonate Hybrid Microspheres for Sustained Delivery of Quetiapine: In-vitro and Ex-vivo Studies

Quetiapine (QTP) is a moderately water-soluble biopharmaceutical classification system (BCS) class II antipsychotic drug used to treat schizophrenia. Carboxymethyl chitosan (CMC) microspheres were prepared using a co-precipitation approach. The Quetiapine was translocated to the brain through a nasal route using carboxymethyl chitosan-calcium carbonate (CMC-CaCO3) hybrid microspheres. During this process, optimization controlled the size of the microspheres by controlling the volume of solution, precipitating agent, and concentration ratios of the precursors. The optimized batch showed a narrow size distribution with high stability in the dispersed phase as characterized by dynamic light scattering. Preliminary characterization revealed crosslinking formation between the carboxymethyl groups of chitosan and the CaCO3 may be responsible for the spherical structure. The preliminary evaluations were done with Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and UV visible spectroscopy (UV-Vis). The surface morphology was assessed by scanning electron microscopy that showed the microscale range of the spherical-shaped particles. The ex-vivo mucoadhesive studies suggested a high binding affinity toward mucin, having a sustained release effect for up to 8 h.

Preliminary Psychometric Evaluation of Novel Measures of Therapist Practice Related to LGBTQ+ Clients.

Culturally competent and equitable mental healthcare for LGBTQ+ people is critical for addressing mental health inequities for this population. Tools to assess therapists' practice with LGBTQ+ clients are needed for research and clinical efforts related to mental healthcare equity goals. We conducted a preliminary assessment of the reliability and validity of a novel 28-item self-report measure assessing therapist practice with LGBTQ+ clients. We examined the construct validity using factor analyses, the convergent and criterion validity using intercorrelations with LGBTQ-affirming knowledge, self-efficacy, and attitudes, and the internal consistency using Cronbach alpha. Our overall total LGBTQ+ practice measure demonstrated excellent internal consistency (Cronbach's alpha = 0.91) and was strongly associated with LGBTQ+ knowledge (rho = 0.377), self-efficacy (rho = 0.633), and LGBTQ+ attitudes (rho = 0.305). We also identified two subscales: "Commitment to Continued Learning" and "Affirmative Practices", which demonstrated similarly strong internal consistency and tests of validity. Our novel measure of overall LGBTQ+ practice, including two subscales, demonstrated strong reliability and validity. These findings have important implications for practice and research in mental healthcare for LGBTQ+ clients. Future research exploring these measures in relationship to mental healthcare outcomes is recommended.

Phytochemical Composition and Toxicological Profiling of Curcuma longa (Turmeric) Root Extract in Rats

Background: Curcuma longa Linn (turmeric) is a common medicinal plant used in Africa for the treatment of diseases. In this study, its extract was evaluated to ascertain its phytochemical composition and toxicity in wistar rats.&#x0D; Methods: The prepared rhizome ethanol extract was first subjected to preliminary phytochemical evaluation and then lethal dose (LD50) test. In the sub-acute toxicity test, 20 adult wistar rats assigned to 4 groups of 5 rats each were administered oral graded doses of the extract. Group 1 served as control while groups 2, 3 and 4 received 250, 500 and 1000 mg/kg body weight of the extract over a period of 28 days, after which blood samples were collected for haematological and serum biochemical analyses.&#x0D; Results: Results obtained from the phytochemical analysis revealed the presence of saponins, tannins, phenolics, flavonoids, steroids, terpenoids, cardiac glycosides and alkaloids in extract, with alkaloids being most abundant (41.20+0.53 mg/100g) and terpenoids, the least (3.98+0.03 mg/100g). Lethal dose value for the extract was found to be &gt;5000mg/kg body weight. Results of sub-acute toxicity evaluation of the extract showed no deleterious effect on liver and kidney function parameters with these parameters being not significantly different from their control values (p&lt;0.05). Treatment with the extract also caused significant hypolipidemia (p&lt;0.05) but increased the values of high-density lipoprotein cholesterol. The activities of antioxidant enzymes and red blood cell counts were also significantly increased following treatment with the extract, especially at higher dose levels.&#x0D; Conclusion: Therefore, we conclude that C. longa extract may be safe for use in the treatment of diseases when administered via the oral route.

Correlation Analysis and Prediction of the Physical and Mechanical Properties of Coastal Soft Soil in the Jiangdong New District, Haikou, China

The compressibility and shear strength of soil play a crucial role in engineering design and construction. For this study, samples were collected from the indoor geotechnical tests conducted on the fourth layer of the third series of the Haikou Formation. By conducting a correlation analysis of various physical properties of soil and utilizing the random forest algorithm, we developed a predictive model for the compressibility and shear strength of coastal soft soil. Initially, we proposed an empirical formula that utilizes mathematical statistical analysis methods to characterize the correlation between the indicators of this soil. Subsequently, we employed the feature selection guided by the aforementioned data analysis results to establish a random forest model. This model predicts the compressive modulus, compressibility coefficient, cohesion, and internal friction angle of the soil. The results indicate that the established model exhibits strong predictive capabilities, with the mean squared error values of compression modulus (0.012), compression coefficient (1.21× 10−6), cohesion (0.081), and internal friction angle (0.003). The data analysis methods, fitting parameters, empirical formulas, and random forest model employed in this study hold substantial value in guiding the preliminary evaluation stage of engineering projects with limited data. This study helps to save time and cost of geotechnical investigation for soft soils in the area.

A privacy-preserving scheme with multi-level regulation compliance for blockchain

With the increasing presence of blockchain-based distributed applications in various aspects of daily life, there has been a growing focus on the privacy protection of blockchain ledgers and the corresponding regulatory technologies. However, current mainstream solutions primarily concentrate on the verifiable encryption of blockchain transaction addresses and contents, neglecting the regulatory requirements for private transactions. Moreover, the few monitorable solutions suffer from issues such as excessive centralization and a single-minded approach to regulatory content. To address these deficiencies, this paper proposes a blockchain privacy-preserving scheme that supports multi-level regulation through the utilization of zero-knowledge proofs (zk-SNARKs) and attribute-based encryption (ABE). Firstly, by leveraging zk-SNARKs, this scheme achieves blockchain privacy-preserving within an account model, enabling the concealment of user transaction addresses and values. Secondly, by employing attribute-based encryption, a multi-level regulatory model is developed alongside the privacy protection measures, allowing for selective disclosure of transaction content. Finally, we analyze the security of the proposed scheme and compare it with other schemes, discussing its advantages in terms of privacy, security, and regulatory capabilities, we also provide a preliminary evaluation of the scheme's efficiency through experiments. In conclusion, the scheme demonstrates strong privacy by relying on mathematical proofs through zk-SNARKs to ensure security while comprehensively safeguarding content. It also achieves multi-level regulation on the foundation of privacy protection, with comprehensive regulatory coverage and decentralized regulatory authority.

A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Mesenchymal stem cell derived exosomes (MSC-Exos) demonstrate beneficial effects on wound healing via anti-inflammatory and angiogenic properties. Chitosan (CS) exhibits excellent biocompatibility and accelerates cellular migration, adhesion, and proliferation. The ions released from bioactive glass (BG) and titanium dioxide (TiO2) nanoparticles exhibit sustained angiogenic and antibacterial potency. In this study, CMCS-CEBT hydrogel was synthesized from exosomes encapsulated carboxymethyl chitosan (CMCS), chitosan nanoparticles (CS-NPs), BG, and TiO2 nanoparticles for a preliminary evaluation of its impacts on the treatment of full-thickness skin defects, diabetic wounds, and burn skin injury due to burns. In vitro analysis indicated that the hydrogel exhibits excellent cell compatibility, stimulates endothelial cell adhesion and proliferation, and presents anti-inflammatory, angiogenic, and antibacterial activities. In vivo, the composite hydrogel dressing accelerated a wound healing acceleration effect, stimulated angiogenesis, and increased collagen deposition and the expression of anti-inflammatory factors. This innovative composite hydrogel dressing as a potential clinical therapy, utilizing bioactive materials, holds promise as a potential clinical therapy that aims to facilitate the regeneration of acute and chronically damaged skin tissue.

Preliminary exploration of herbal tea products based on traditional knowledge and hypotheses concerning herbal tea selection: a case study in Southwest Guizhou, China.

Herbal tea usually refers to "beverage plants that do not belong to the genus Camellia", and it holds a significant historical legacy as a traditional beverage among specific regions and ethnic groups. In light of this, our research aims to investigate and analyze the traditional knowledge pertaining to herbal tea plants used by local people in the QianxinanBuyi and Miao Autonomous Prefecture, Guizhou Province. We also initiated preliminary efforts to create tea products from herbal tea leaves using various processing techniques. Additionally, we attempted to test hypotheses to elucidate how local people select herbal tea plants. Data related to the use of herbal tea plants in this study were collected through semi-structured interviews and participatory observations in four villages in Qianxinan. Quantitative indicators, including the relative frequency of citation (RFC) and the relative importance (RI) value, were calculated, and the availability of plants was also evaluated. General linear model was performed to examine the relationship between the frequency of citation and resource availability, as well as the correlation between the relative frequency of citation and the relative importance, to test both the resource availability hypothesis and the versatility hypothesis. Centella asiatica tea was processed using techniques from green tea, black tea and white tea, with a preliminary sensory evaluation conducted. A total of 114 plant species were documented as being used for herbal teas by local residents, representing 60 families and 104 genera. Of these, 61% of herbal tea plants were found growing in the wild, and 11 species were exotic plants. The family with the highest number of species was Asteraceae (20 species). The study identified 33 major medicinal functions of herbal tea, with clearing heat-toxin and diuresis being the most common functions. General linear model revealed a strong correlation (correlation coefficient of 0.72, p < 0.001) between the frequency of citation and plant availability, as well as a significant correlation (correlation coefficient of 0.63, p < 0.001) between RFC and RI. Under different processing conditions, the characteristics of Centella asiatica tea exhibited variations and were found to be suitable for consumption. The consumption of herbal tea serves as a preventive measure against common ailments for local residents. The resource availability hypothesis, diversification hypothesis and the versatility hypothesis were shown to provide some insight into "how and why local communities select plants for use." Exotic herbal tea plants in the study area also possess valuable therapeutic properties. The processing and production of Centella asiatica herbal tea products hold promising prospects.

Suspect screening analysis to improve untargeted and targeted UHPLC-qToF approaches: the biodegradability of a proton pump inhibitor medicine and a natural medical device

Suspect screening and untargeted analysis using UHPLC-qToF are two advanced analytical approaches now used to achieve an extensive chemical profile of samples, which are then typically confirmed through targeted analysis. These techniques can detect a large number of chemical features simultaneously and are currently being introduced into the study of contaminants of emerging concern (CECs) and into the study of the extent of human chemical exposure (the exposome). Here is described the use of these techniques to characterize chemical mixtures derived from the OECD 301F ready biodegradability test (RBT) of a chemical and natural formulation currently used to treat reflux disease and functional dyspepsia. Untargeted analysis clearly evidenced a different behavior between formulations containing only natural products with respect to that containing synthetic and non-naturally occurring substances. Suspect screening analysis improved the untargeted analysis of the omeprazole-based medicine, leading to the tentative identification of a number of omeprazole-derived transformation products, thereby enabling their preliminary quali-quantitative evaluation. Targeted analysis was then performed to confirm the preliminary data gained from the suspect screening approach. The validation of the analytical method for the quantitative determination of omeprazole and its major metabolite, omeprazole sulphide, has provided robust data to evaluate the behavior of omeprazole during the OECD 301F test. Using advanced analytical approaches, the RBT performed on the two products under investigation confirmed that omeprazole is not readily biodegradable, while the medical device made of natural substances has proven to be readily biodegradable.

ADVANCED IN VITRO 3D TISSUE CULTURE MODEL FOR THE ASSESSMENT OF TITANIUM IMPLANT OSTEOINTEGRATION

The use of implant biomaterials for prosthetic reconstructive surgery and osteosynthesis is consolidated in the orthopaedic field, improving the quality of life of patients and allowing for healthy and better ageing. However, there is the lack of advanced innovative methods to investigate the potentialities of smart biomaterials, particularly for the study of local effects of implant and osteointegration. Despite the complex process of osseointegration is difficult to recreate in vitro, the growing challenges in developing alternative models require to set-up and validate new approaches. Aim of the present study is to evaluate an advanced in vitro tissue culture model of osteointegration of titanium implants in human trabecular bone. Cubic samples (1.5×1.5 cm) of trabecular bone were harvested as waste material from hip arthroplasty surgery (CE AVEC 829/2019/Sper/IOR); cylindrical defects (2 mm Ø, 6 mm length) were created, and tissue specimens assigned to the following groups: 1) empty defects- CTR-; 2) defects implanted with a cytotoxic copper pin (Merck cod. 326429)- CTR+; 3) defects implanted with standard titanium pins of 6 µm-rough (ZARE S.r.l) -Ti6. Tissue specimens were cultured in mini rotating bioreactors in standard conditions, weekly assessing viability. At the 8-week-timepoint, immunoenzymatic, microtomographic, histological and histomorphometric analyses were performed. The model was able to simulate the effects of implantation of the materials, showing a drop in viability in CTR+, differently from Ti6 which appears to have a trophic effect on the bone. MicroCT and histological analysis supported the results, with lower BV/TV and Tb.Th values observed in CTR- compared to CTR+ and Ti6 and signs of matrix and bone deposition at the implant site. The collected data suggest the reliability of the tested model which can recreate the osseointegration process in vitro and can therefore be used for preliminary evaluations to reduce and refine in vivo preclinical models.Acknowledgment: This work was supported by Emilia-Romagna Region for the project “Sviluppo di modelli biologici in vitro ed in silico per la valutazione e predizione dell'osteointegrazione di dispositivi medici da impianto nel tessuto osseo”

Digital Evidence in Disputes Involving States

The use of digital evidence increases concurrently with increased digitalization of the larger world and of justice processes. This essay aims to address the use of digital evidence in interstate disputes and other disputes involving states. It focuses on the case law of two international arbitral bodies—the Permanent Court of Arbitration (PCA) and the International Centre for Settlement of Investment Disputes (ICSID)—and of the International Court of Justice (ICJ). The analysis discloses three main concerns when dealing with digital evidence: authorship, authenticity, and chain of custody. We propose that courts create permanent and ad hoc digital forensic expert committees to draft guidelines and perform a preliminary admissibility evaluation of digital evidence.